Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,184 G→A 23.6% intergenic (‑160/+116) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1840G→A23.6% 82.9 ‑0.9 0.0135intergenic (‑160/+116)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/17):  ref base (40/63):  total (55/80)
Fisher's exact test strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

GTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTT  >  NC_014034/2640085‑2640283
                                                                                                       |                                                                                                   
gTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAgg                                                                                                     <  2:3356069/101‑1 (MQ=255)
    gCGACGCGGCGGGAGGCGTGTCAACCGGGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTAtt                                                                                                 <  1:1434330/101‑1 (MQ=25)
    gCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTAtt                                                                                                 <  2:756326/101‑1 (MQ=255)
             cggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTTGGG.C.TTCGGCCCATTCACCCCCCCAGGGATTTTGaggaag                                                                                        >  2:3682733/1‑96 (MQ=25)
              ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                       <  1:345314/101‑1 (MQ=37)
               gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                      <  2:3801667/101‑1 (MQ=37)
                cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                     <  2:1805999/101‑1 (MQ=37)
                  ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                   <  1:2184471/101‑1 (MQ=25)
                  ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCC.CTT.GG.CGCTTGGGCCATTTACCCCCCCAGGTATTTGGAGGAAGATGaa                                                                                   >  1:2089534/1‑101 (MQ=255)
                   gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                  <  1:1897055/101‑1 (MQ=25)
                    cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                 <  1:58641/101‑2 (MQ=25)
                       gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                              <  1:1109101/101‑5 (MQ=25)
                       gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                              <  2:4104802/101‑5 (MQ=25)
                          aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                          <  2:355009/101‑1 (MQ=21)
                           aCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                         <  1:1652240/101‑1 (MQ=25)
                               tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTTCGT.C.TTTGGCCAATTCAGCCTCCGAGGTATTTTTGCCAAGATG.CAAGAGCATGGGTTc                                                                     >  2:3141611/1‑101 (MQ=16)
                               tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                     <  2:553602/101‑1 (MQ=21)
                               gggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                     <  2:2139839/100‑1 (MQ=25)
                                ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                    <  2:2789973/101‑1 (MQ=25)
                                 gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                   <  2:898430/101‑1 (MQ=25)
                                  cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCATCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATc                                                                  >  1:1989832/1‑101 (MQ=21)
                                    cTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                <  1:2347939/101‑1 (MQ=25)
                                     ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                               <  2:2517352/101‑1 (MQ=25)
                                         gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                           <  1:2311517/101‑1 (MQ=21)
                                         gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                           <  2:2584354/101‑1 (MQ=25)
                                          cgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCa                                                          <  2:3073057/101‑1 (MQ=25)
                                           gcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaa                                                         <  2:843951/101‑1 (MQ=25)
                                            gccccgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                       <  2:2641040/95‑1 (MQ=14)
                                             agccAAAAGGGGGCCGCTGCCCCCCT.CTT.GGTC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                       <  2:746158/98‑1 (MQ=25)
                                              ggCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                      <  2:3575984/101‑1 (MQ=21)
                                              cccgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                     <  2:2473130/97‑1 (MQ=17)
                                                cgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                   >  1:1204884/3‑101 (MQ=17)
                                                cgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                   <  2:575675/99‑1 (MQ=17)
                                                cgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                   >  2:4119652/3‑101 (MQ=17)
                                                 gaaaAGGGGGCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTANCTTGCCCCAAATATccc                                                  >  2:1778669/2‑101 (MQ=17)
                                                  aaaGGGGGGC.CCGCCCCCCTCCTT.GG.C.CTCCGGCAAATCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                 <  1:3006725/101‑1 (MQ=21)
                                                  aaaAGGGGGCCCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                 <  1:258985/101‑1 (MQ=18)
                                                   aaagggggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                >  2:3705510/4‑101 (MQ=17)
                                                     agggggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                              >  1:1356112/2‑101 (MQ=18)
                                                      gggggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                              <  2:2731094/101‑1 (MQ=25)
                                                        gggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                            <  2:2321714/101‑1 (MQ=25)
                                                        gggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                           >  2:4392741/1‑99 (MQ=18)
                                                        gggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                           <  2:2966740/101‑3 (MQ=18)
                                                         ggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                          <  1:4092162/101‑4 (MQ=21)
                                                         ggCGCTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCggggggga                                          >  2:2151925/1‑99 (MQ=18)
                                                         gccgcTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                          <  1:1084325/99‑4 (MQ=21)
                                                          gcgcTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                         <  1:4293630/101‑5 (MQ=21)
                                                          gcgcTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                         <  1:3417906/101‑5 (MQ=21)
                                                           cgcTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                        <  2:240521/101‑6 (MQ=21)
                                                             ttGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                       <  1:2032567/100‑1 (MQ=25)
                                                             cTGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.TTCTTGCCCCCAATATCCCgggggggaggcg                                      >  1:1756701/1‑95 (MQ=18)
                                                              tGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGCGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCCAATATCCCGGGGGGGGGCCt                                      >  2:1749875/1‑101 (MQ=21)
                                                              tGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcca                                      >  2:2032427/1‑100 (MQ=25)
                                                              tGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                      <  1:3683008/101‑1 (MQ=25)
                                                              tGCCCCCCT.CTT.GGCC.TTCGGCCAATTCACCCCCCCGGAGATTTGCGGCAAGGTGAAAGGA..TGGGGTATATCTTGCCCCCAATATCCCgagggtgcggcga                                     >  2:317634/1‑93 (MQ=18)
                                                                ccccccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                    >  2:1899673/1‑101 (MQ=25)
                                                                ccccccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                    >  2:1541300/1‑101 (MQ=25)
                                                                 cccccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                   >  1:1261680/1‑101 (MQ=25)
                                                                   cccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                 >  1:2889481/1‑101 (MQ=37)
                                                                    ccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                >  1:462203/1‑101 (MQ=37)
                                                                    ccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                <  1:1402267/101‑1 (MQ=37)
                                                                    ccT.CTT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                <  1:3501361/101‑1 (MQ=37)
                                                                     ct.ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                               >  2:3327683/1‑101 (MQ=37)
                                                                     ct.ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                               >  1:3527331/1‑101 (MQ=37)
                                                                      t.ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                              <  1:1242583/101‑1 (MQ=37)
                                                                      t.ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                              <  1:2882076/101‑1 (MQ=37)
                                                                      t.ctT.GGCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                              >  1:3813506/1‑101 (MQ=37)
                                                                           tggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                           >  2:1307922/5‑101 (MQ=37)
                                                                             gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                         <  2:1989697/99‑1 (MQ=37)
                                                                              cc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                        >  1:4620347/2‑101 (MQ=37)
                                                                               c.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                       >  1:4438987/1‑101 (MQ=37)
                                                                                 ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                      <  2:3205341/101‑1 (MQ=255)
                                                                                 ttCGGACAAGTCACCCCCCGAGGTATTTGGGGCAAGATGAAAA.ACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                      <  2:1841463/101‑1 (MQ=25)
                                                                                  tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                     >  2:1080611/1‑101 (MQ=37)
                                                                                  tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                     <  1:4496931/101‑1 (MQ=255)
                                                                                    ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                   <  1:1063811/101‑1 (MQ=255)
                                                                                    ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                   >  1:1294296/1‑101 (MQ=37)
                                                                                     gCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                  <  1:3351117/101‑1 (MQ=255)
                                                                                     gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                  >  2:158525/1‑101 (MQ=37)
                                                                                       cAATTCACCCACCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                >  2:2318962/1‑101 (MQ=25)
                                                                                        aaTTCACCACCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCCTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc               >  2:1652292/1‑101 (MQ=37)
                                                                                         aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc              >  2:2746508/1‑101 (MQ=37)
                                                                                         aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc              >  1:4191721/1‑101 (MQ=255)
                                                                                          ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc             <  2:1371737/101‑1 (MQ=255)
                                                                                           tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt            >  1:914169/4‑101 (MQ=25)
                                                                                            caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc           <  2:4109499/99‑1 (MQ=255)
                                                                                             cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc          <  2:2067508/100‑1 (MQ=255)
                                                                                             accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc          >  2:1366748/2‑101 (MQ=255)
                                                                                              ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc         <  1:1749994/101‑1 (MQ=255)
                                                                                              cccaccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc         <  1:2319124/101‑1 (MQ=255)
                                                                                                  ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt     >  1:1167436/1‑101 (MQ=255)
                                                                                                  ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt     >  1:3175284/1‑101 (MQ=255)
                                                                                                  ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt     >  2:426744/1‑101 (MQ=255)
                                                                                                    gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt   >  1:4412355/1‑101 (MQ=255)
                                                                                                    gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt   >  2:34989/1‑101 (MQ=255)
                                                                                                    gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt   >  2:2066322/1‑101 (MQ=255)
                                                                                                     aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt  <  1:2129859/101‑1 (MQ=255)
                                                                                                     aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt  >  1:2481085/1‑101 (MQ=255)
                                                                                                     aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt  >  1:4329800/1‑101 (MQ=255)
                                                                                                     aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt  >  2:289868/1‑101 (MQ=255)
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GTATGCGACGCGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GG.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTT  >  NC_014034/2640085‑2640283

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG