Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,198 A→G 19.5% intergenic (‑174/+102) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1980A→G19.5% 78.6 0.0 ‑0.0159intergenic (‑174/+102)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/16):  ref base (62/66):  total (77/82)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.97e-01

Only 100 of 164 total aligned reads displayed.

GGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.G.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640099‑2640297
                                                                                                        |                                                                                                     
ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                                       <  1:345314/101‑1 (MQ=37)
 gccagCGTGTGAACCGTGGCGCTTTTGCTCAGGCGAAAGGGGGGCTGTGCCCCCCT.CTT.G.GCC.TTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                                      <  2:513021/97‑1 (MQ=21)
 gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                                      <  1:1820549/101‑1 (MQ=37)
 gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                                      <  2:3801667/101‑1 (MQ=37)
  cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                                     <  1:2232739/101‑1 (MQ=25)
   aGGCGTATCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCGCCCCT.CTT.G.GCC.TTCGGCCATTTCACCCCCCGAGGTACTTGGAGCAAGACGa                                                                                                    <  1:2659316/101‑1 (MQ=25)
    ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCC.CTT.G.G.CGCTTGGGCCATTTACCCCCCCAGGTATTTGGAGGAAGATGaa                                                                                                   >  1:2089534/1‑101 (MQ=255)
     gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                                  <  1:1897055/101‑1 (MQ=25)
      cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCT.CTTCT.C.C.TTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATGaaaa                                                                                                 >  2:1682965/1‑101 (MQ=25)
         gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                              <  2:4104802/101‑5 (MQ=25)
         gtCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaagacc                                                                                              <  1:1109101/101‑5 (MQ=25)
           catccGTGGCGCTTTTGCGCACGCCATATCGGGGCGCAGCGCCCCT.CTT.GCA.C.TTAGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATGaaatgccat                                                                                            <  2:2492458/98‑7 (MQ=255)
            aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                          <  2:355009/101‑1 (MQ=21)
                 tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTTCG.T.C.TTTGGCCAATTCAGCCTCCGAGGTATTTTTGCCAAGATG.CAAGAGCATG..GGTTc                                                                                   >  2:3141611/1‑101 (MQ=16)
                 tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                                                   <  1:2114179/101‑1 (MQ=21)
                   gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCAt                                                                                 <  2:898430/101‑1 (MQ=25)
                      cTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                                              <  1:2347939/101‑1 (MQ=25)
                           gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                         <  1:2311517/101‑1 (MQ=21)
                           gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                         <  2:2584354/101‑1 (MQ=25)
                            cgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCa                                                                        <  2:3073057/101‑1 (MQ=25)
                               agccAAAAGGGGGCCGCTGCCCCCCT.CTT.G.GTC.TTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                                                     <  2:746158/98‑1 (MQ=25)
                                ggCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                                                    <  2:3575984/101‑1 (MQ=21)
                                cccgaaaAGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAatat                                                                   <  2:2473130/97‑1 (MQ=17)
                                  cgaaaAGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATcc                                                                 <  2:575675/99‑1 (MQ=17)
                                   gaaaAGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                                                <  1:1215644/100‑1 (MQ=17)
                                   gaaaAGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTANCTTGCCCCAAATATccc                                                                >  2:1778669/2‑101 (MQ=17)
                                    aaaGGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                                <  1:518442/101‑1 (MQ=25)
                                    aaaGGGGGGC.CCGCCCCCCTCCTT.G.G.C.CTCCGGCAAATCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCg                                                               <  1:3006725/101‑1 (MQ=21)
                                    aaaAGGGGGCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                               <  2:2748614/101‑1 (MQ=17)
                                    aaaAGGGGGCCCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                               <  1:258985/101‑1 (MQ=18)
                                     aaagggggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                              >  2:3705510/4‑101 (MQ=17)
                                       agggggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                            >  1:1356112/2‑101 (MQ=18)
                                       agggggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                            >  2:1578326/2‑101 (MQ=18)
                                        gggggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                                            <  2:1352989/101‑1 (MQ=25)
                                        gggggCGCTG.CCCCCT.CTT.G.GCC.TTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg                                                          <  2:2120767/101‑1 (MQ=2)
                                          gggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggggg                                                          <  2:2321714/101‑1 (MQ=25)
                                          gggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                         >  2:4392741/1‑99 (MQ=18)
                                          gggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                         >  1:4408096/1‑99 (MQ=18)
                                          gggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggggg                                                          >  2:2721403/1‑101 (MQ=25)
                                           ggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                        <  1:4092162/101‑4 (MQ=21)
                                           ggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGG..GGTT.ATCTTGCCCCAAATATCCCggggggga                                                        >  2:2151925/1‑99 (MQ=18)
                                           ggCGCTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                         <  2:1026362/101‑1 (MQ=25)
                                           gccgcTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                        <  1:1084325/99‑4 (MQ=21)
                                            gcgcTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                       <  1:4293630/101‑5 (MQ=21)
                                            gcgcTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                       <  2:4149903/101‑5 (MQ=21)
                                             cgcTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      <  2:3939473/101‑6 (MQ=21)
                                             cgcTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                      <  2:240521/101‑6 (MQ=21)
                                               cTGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                    >  1:2889601/1‑94 (MQ=25)
                                                tGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcca                                                    >  2:2032427/1‑100 (MQ=25)
                                                tGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                                    <  1:3683008/101‑1 (MQ=25)
                                                tGCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCCGGAGATTTGCGGCAAGGTGAAAGGA..TGG..GGTATATCTTGCCCCCAATATCCCgagggtgcggcga                                                   >  2:317634/1‑93 (MQ=18)
                                                 gCCCCCCT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggggaggcgaa                                                  >  1:127641/1‑93 (MQ=25)
                                                  ccccccT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                                  >  2:1899673/1‑101 (MQ=25)
                                                   cccccT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                 >  1:1261680/1‑101 (MQ=25)
                                                     cccT.CTT.G.GAC.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                               <  1:2032281/101‑1 (MQ=37)
                                                      ccT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              <  1:1402267/101‑1 (MQ=37)
                                                      ccT.CTT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              <  1:3501361/101‑1 (MQ=37)
                                                       ct.ctT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  2:3327683/1‑101 (MQ=37)
                                                        t.ctT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                            >  1:3813506/1‑101 (MQ=37)
                                                        t.ctT.G.GCC.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                            <  1:2882076/101‑1 (MQ=37)
                                                              tggcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  2:1307922/5‑101 (MQ=37)
                                                                gcc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                       <  2:1989697/99‑1 (MQ=37)
                                                                 cc.TTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                      >  1:4620347/2‑101 (MQ=37)
                                                                    ttCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                    <  2:3205341/101‑1 (MQ=255)
                                                                    ttCGGACAAGTCACCCCCCGAGGTATTTGGGGCAAGATGAAAA.ACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                    <  2:1841463/101‑1 (MQ=25)
                                                                     tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                   <  1:4496931/101‑1 (MQ=255)
                                                                       ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 <  1:1063811/101‑1 (MQ=255)
                                                                        gCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                <  1:3351117/101‑1 (MQ=255)
                                                                        gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                >  2:158525/1‑101 (MQ=37)
                                                                            aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            >  1:4191721/1‑101 (MQ=255)
                                                                            aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            >  2:2746508/1‑101 (MQ=37)
                                                                             ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                           <  2:1371737/101‑1 (MQ=255)
                                                                              tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  1:914169/4‑101 (MQ=25)
                                                                               caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         <  2:4109499/99‑1 (MQ=255)
                                                                                 ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  1:1749994/101‑1 (MQ=255)
                                                                                   ccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                     >  2:2959146/1‑101 (MQ=255)
                                                                                     ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                   >  1:1167436/1‑101 (MQ=255)
                                                                                       gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 >  1:4412355/1‑101 (MQ=255)
                                                                                       gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 >  2:2066322/1‑101 (MQ=255)
                                                                                       gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCt                 >  2:1794586/1‑101 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  2:289868/1‑101 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  1:2481085/1‑101 (MQ=255)
                                                                                        aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                >  1:1691652/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:756393/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:3314646/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:4578592/1‑101 (MQ=255)
                                                                                           tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  2:3426415/1‑101 (MQ=255)
                                                                                            aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGAACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            >  1:1498210/1‑101 (MQ=255)
                                                                                             tttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           >  1:3473510/1‑101 (MQ=255)
                                                                                              ttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          >  1:3563661/1‑101 (MQ=255)
                                                                                               tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         >  1:2135655/1‑101 (MQ=255)
                                                                                                ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:2639042/1‑101 (MQ=255)
                                                                                                ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        >  2:1075966/1‑101 (MQ=255)
                                                                                                 gagcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  2:743558/4‑101 (MQ=255)
                                                                                                 gagcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg       >  1:3223233/4‑101 (MQ=255)
                                                                                                  agcAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGt      >  1:2821026/3‑101 (MQ=255)
                                                                                                    cAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  1:1454864/1‑101 (MQ=255)
                                                                                                     aaGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGTGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc   >  2:2311959/1‑101 (MQ=255)
                                                                                                      aGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  1:789907/1‑101 (MQ=255)
                                                                                                      aGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  >  2:3689162/1‑101 (MQ=255)
                                                                                                        |                                                                                                     
GGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.G.C.TTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640099‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG