Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,204 A→G 21.3% intergenic (‑180/+96) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2040A→G21.3% 77.6 ‑0.4 0.0146intergenic (‑180/+96)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/16):  ref base (62/52):  total (77/69)
Fisher's exact test strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

CGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGA  >  NC_014034/2640105‑2640304
                                                                                                      |                                                                                                    
cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCT.CTTCTC.CTTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATGaaaa                                                                                                      >  2:1682965/1‑101 (MQ=25)
      aaCCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                               <  2:355009/101‑1 (MQ=21)
           gggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                          <  2:2139839/100‑1 (MQ=25)
            ggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                         <  2:2789973/101‑1 (MQ=25)
             gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                        <  2:898430/101‑1 (MQ=25)
              cgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCATCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATc                                                                                       >  1:1989832/1‑101 (MQ=21)
                cTTGTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCtt                                                                                     <  1:2347939/101‑1 (MQ=25)
                 ttttGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                                    <  2:2517352/101‑1 (MQ=25)
                     gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                <  1:2311517/101‑1 (MQ=21)
                         agccAAAAGGGGGCCGCTGCCCCCCT.CTT.GGTCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                            <  2:746158/98‑1 (MQ=25)
                          ggCAAAAGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAata                                                                           <  2:3575984/101‑1 (MQ=21)
                            cgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                        <  2:575675/99‑1 (MQ=17)
                            cgaaaAGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                        >  1:1204884/3‑101 (MQ=17)
                             gaaaAGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTANCTTGCCCCAAATATccc                                                                       >  2:1778669/2‑101 (MQ=17)
                              aaaGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                       <  2:3189647/101‑1 (MQ=21)
                              aaaGGGGGGCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                       <  1:518442/101‑1 (MQ=25)
                              aaaGGGGGGC.CCGCCCCCCTCCTT.GG.CCTCCGGCAAATCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCg                                                                      <  1:3006725/101‑1 (MQ=21)
                               aaagggggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                                     >  2:3705510/4‑101 (MQ=17)
                                 agggggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                   >  2:1578326/2‑101 (MQ=18)
                                  gggggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                                   <  2:1352989/101‑1 (MQ=25)
                                    gggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                >  2:4392741/1‑99 (MQ=18)
                                    gggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                >  2:3699403/1‑99 (MQ=18)
                                    gggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                <  2:2966740/101‑3 (MQ=18)
                                    gggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                 >  2:2721403/1‑101 (MQ=25)
                                     ggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                               <  1:4092162/101‑4 (MQ=21)
                                     ggCGCTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                <  2:1026362/101‑1 (MQ=25)
                                     gccgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                               <  1:1084325/99‑4 (MQ=21)
                                      gcgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                              <  1:3417906/101‑5 (MQ=21)
                                      gcgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                              <  2:4149903/101‑5 (MQ=21)
                                      gcgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgag                                                              <  1:4293630/101‑5 (MQ=21)
                                       cgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                             <  2:3939473/101‑6 (MQ=21)
                                       cgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                             >  2:1877863/1‑96 (MQ=21)
                                       cgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                             <  2:2002466/101‑6 (MQ=21)
                                       cgcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                             <  2:240521/101‑6 (MQ=21)
                                        gcTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                            >  1:3459872/1‑95 (MQ=21)
                                         ttGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                            <  1:2032567/100‑1 (MQ=25)
                                         cTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGGTATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.TTCTTGCCCCCAATATCCCgggggggaggcg                                                           >  1:1756701/1‑95 (MQ=18)
                                         cTGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                           >  1:2889601/1‑94 (MQ=25)
                                          tGCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCCGGAGATTTGCGGCAAGGTGAAAGGA..TGGGGTATATCTTGCCCCCAATATCCCgagggtgcggcga                                                          >  2:317634/1‑93 (MQ=18)
                                           gCCCCCCT.CTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggggaggcgaa                                                         >  1:127641/1‑93 (MQ=25)
                                               cccT.CTT.GGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGTCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                      <  1:3644658/101‑1 (MQ=25)
                                                ccT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                     >  1:462203/1‑101 (MQ=37)
                                                ccT.CTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                                     <  1:3501361/101‑1 (MQ=37)
                                                  t.ctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                   <  1:2882076/101‑1 (MQ=37)
                                                  t.ctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                   >  1:3813506/1‑101 (MQ=37)
                                                  t.ctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                   <  1:1242583/101‑1 (MQ=37)
                                                       tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                >  2:1307922/5‑101 (MQ=37)
                                                         gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                              <  2:1989697/99‑1 (MQ=37)
                                                          ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                             >  1:4620347/2‑101 (MQ=37)
                                                             tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                          <  1:4496931/101‑1 (MQ=255)
                                                               ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                        >  1:1294296/1‑101 (MQ=37)
                                                               ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                        <  1:1063811/101‑1 (MQ=255)
                                                                gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                       >  2:158525/1‑101 (MQ=37)
                                                                 ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                                      >  1:2359969/1‑101 (MQ=37)
                                                                  cAATTCACCCACCGAGGGATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                                     >  2:2318962/1‑101 (MQ=25)
                                                                    aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                   >  2:2746508/1‑101 (MQ=37)
                                                                    aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                                   >  1:4191721/1‑101 (MQ=255)
                                                                     ttCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccccc                                  <  2:1371737/101‑1 (MQ=255)
                                                                      tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                 >  1:914169/4‑101 (MQ=25)
                                                                       caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                <  2:4109499/99‑1 (MQ=255)
                                                                       caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                >  1:1358911/3‑101 (MQ=255)
                                                                        cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                               <  2:2067508/100‑1 (MQ=255)
                                                                             ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                          >  2:426744/1‑101 (MQ=255)
                                                                             ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                          >  1:1167436/1‑101 (MQ=255)
                                                                             ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                          >  1:3175284/1‑101 (MQ=255)
                                                                               gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                        >  2:2066322/1‑101 (MQ=255)
                                                                               gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                        >  1:4412355/1‑101 (MQ=255)
                                                                               gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCt                        >  2:1794586/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                       >  1:1691652/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                       >  2:289868/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                       >  2:3117512/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                       >  1:4329800/1‑101 (MQ=255)
                                                                                aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                       <  1:2129859/101‑1 (MQ=255)
                                                                                   tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                    >  1:756393/1‑101 (MQ=255)
                                                                                   tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                    >  1:4578592/1‑101 (MQ=255)
                                                                                   tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                    >  1:3314646/1‑101 (MQ=255)
                                                                                   tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                    >  2:3426415/1‑101 (MQ=255)
                                                                                    aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc                   >  2:2473816/1‑101 (MQ=255)
                                                                                     tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                  >  2:3242799/1‑101 (MQ=255)
                                                                                      ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                 >  1:3563661/1‑101 (MQ=255)
                                                                                      ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                 <  1:2721600/101‑1 (MQ=255)
                                                                                       tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                <  1:2606822/101‑1 (MQ=255)
                                                                                       tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                >  1:2135655/1‑101 (MQ=255)
                                                                                        ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc               >  1:1476741/1‑101 (MQ=255)
                                                                                        ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc               >  2:2639042/1‑101 (MQ=255)
                                                                                        ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc               >  2:1075966/1‑101 (MQ=255)
                                                                                         gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg              >  2:743558/4‑101 (MQ=255)
                                                                                         gagcAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCg              >  1:3223233/4‑101 (MQ=255)
                                                                                            cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc           >  1:1454864/1‑101 (MQ=255)
                                                                                             aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGTGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc          >  2:2311959/1‑101 (MQ=255)
                                                                                              aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg         >  1:789907/1‑101 (MQ=255)
                                                                                                gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg        >  2:4115085/5‑101 (MQ=255)
                                                                                                gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGGCTTTCTCAAGCGTTCCgg        >  2:3614084/5‑101 (MQ=255)
                                                                                                 atgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGc       >  1:4487681/4‑101 (MQ=255)
                                                                                                  tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt      >  1:2506516/3‑101 (MQ=255)
                                                                                                   gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc     >  1:1574350/2‑101 (MQ=255)
                                                                                                    aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa    >  2:3014232/1‑101 (MQ=255)
                                                                                                    aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa    >  2:2651670/1‑101 (MQ=255)
                                                                                                     aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAg   >  2:3441492/1‑101 (MQ=255)
                                                                                                      aGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGa  <  1:1683076/101‑1 (MQ=255)
                                                                                                      |                                                                                                    
CGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGA  >  NC_014034/2640105‑2640304

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG