Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,225 T→C 16.6% intergenic (‑201/+75) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2250T→C16.6% 76.7 ‑2.8 0.0189intergenic (‑201/+75)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/16):  ref base (106/51):  total (121/68)
Fisher's exact test strand distribution p-value = 6.33e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 193 total aligned reads displayed.

GCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCACCT  >  NC_014034/2640126‑2640325
                                                                                                     |                                                                                                    
gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTTAGC.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                                     <  1:4563587/101‑1 (MQ=25)
gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                                     <  1:2311517/101‑1 (MQ=21)
gcgcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                                                     <  2:2584354/101‑1 (MQ=25)
  gcAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaa                                                                                                   <  2:843951/101‑1 (MQ=25)
    agccAAAAGGGGGCCGCTGCCCCCCTCTT.GGTCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                                                 <  2:746158/98‑1 (MQ=25)
     cccgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAatat                                                                                               <  2:2473130/97‑1 (MQ=17)
       cgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                                             <  2:575675/99‑1 (MQ=17)
       cgaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATcc                                                                                             >  2:4119652/3‑101 (MQ=17)
        gaaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATccc                                                                                            <  1:1215644/100‑1 (MQ=17)
         aaaGGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATccc                                                                                            <  2:3189647/101‑1 (MQ=21)
         aaaAGGGGGCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                           <  2:2748614/101‑1 (MQ=17)
         aaaAGGGGGCCCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                                                           <  1:258985/101‑1 (MQ=18)
            agggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                                        >  1:1356112/2‑101 (MQ=18)
            agggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggg                                                                                        >  2:1578326/2‑101 (MQ=18)
             gggggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggg                                                                                        <  2:2731094/101‑1 (MQ=25)
               gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACGCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCgggggg                                                                                      <  2:2321714/101‑1 (MQ=25)
               gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                                     >  2:3699403/1‑99 (MQ=18)
               gggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtg                                                                                     >  1:4408096/1‑99 (MQ=18)
                ggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGGGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCggggggga                                                                                    >  2:2151925/1‑99 (MQ=18)
                ggCGCTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                                                     <  2:1026362/101‑1 (MQ=25)
                gccgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtga                                                                                    <  1:1084325/99‑4 (MQ=21)
                  cgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                                  <  2:3939473/101‑6 (MQ=21)
                  cgcTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                                                  >  2:1877863/1‑96 (MQ=21)
                    ttGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                                                                 <  1:2032567/100‑1 (MQ=25)
                    cTGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                                                                >  1:2889601/1‑94 (MQ=25)
                     tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGCGGTATTTTGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCCAATATCCCGGGGGGGGGCCt                                                                                >  2:1749875/1‑101 (MQ=21)
                     tGCCCCCCTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcca                                                                                >  2:2032427/1‑100 (MQ=25)
                        cccccTCTT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaa                                                                             >  1:1261680/1‑101 (MQ=25)
                          cccTCTT.GGCCTTCGGTCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGTCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                                           <  1:3644658/101‑1 (MQ=25)
                          cccTCTT.GGACTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                                                           <  1:2032281/101‑1 (MQ=37)
                            ctctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                                                         >  1:3527331/1‑101 (MQ=37)
                             tctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                                        <  1:2882076/101‑1 (MQ=37)
                             tctT.GGCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                                                        >  1:3813506/1‑101 (MQ=37)
                                 tggccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                                                     >  2:1307922/5‑101 (MQ=37)
                                   gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                                   <  2:1989697/99‑1 (MQ=37)
                                   gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                                                   <  2:899667/99‑1 (MQ=37)
                                    ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                                                  >  1:4620347/2‑101 (MQ=37)
                                     cTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                                                 >  1:4438987/1‑101 (MQ=37)
                                       tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                                               >  2:1080611/1‑101 (MQ=37)
                                       tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                                               <  1:4496931/101‑1 (MQ=255)
                                         ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                                             <  1:1063811/101‑1 (MQ=255)
                                          gCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                                            <  1:3351117/101‑1 (MQ=255)
                                             aaTTCACCACCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCCTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                                                         >  2:1652292/1‑101 (MQ=37)
                                                tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                                                      >  1:914169/4‑101 (MQ=25)
                                                 caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                                                     <  2:4109499/99‑1 (MQ=255)
                                                  cccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                                    <  2:2067508/100‑1 (MQ=255)
                                                  accccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                                                    >  2:1366748/2‑101 (MQ=255)
                                                   ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                                   <  1:1749994/101‑1 (MQ=255)
                                                   cccaccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                                                   <  1:2319124/101‑1 (MQ=255)
                                                    cccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                                                  >  2:3565847/1‑101 (MQ=255)
                                                     ccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg                                                 >  2:2959146/1‑101 (MQ=255)
                                                       ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                                               >  2:426744/1‑101 (MQ=255)
                                                       ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                                               >  1:3175284/1‑101 (MQ=255)
                                                       ccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt                                               >  1:1167436/1‑101 (MQ=255)
                                                         gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCt                                             >  2:1794586/1‑101 (MQ=255)
                                                          aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                            >  1:1691652/1‑101 (MQ=255)
                                                          aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                                            >  1:2481085/1‑101 (MQ=255)
                                                             tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                         >  1:3314646/1‑101 (MQ=255)
                                                             tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct                                         >  2:3426415/1‑101 (MQ=255)
                                                              aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCATGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTATTtatc                                        >  1:860266/1‑101 (MQ=25)
                                                               tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                                       >  1:3473510/1‑101 (MQ=255)
                                                               tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa                                       >  2:3242799/1‑101 (MQ=255)
                                                                ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa                                      <  1:2721600/101‑1 (MQ=255)
                                                                 tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg                                     >  1:2135655/1‑101 (MQ=255)
                                                                  ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc                                    >  2:2639042/1‑101 (MQ=255)
                                                                      cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc                                >  1:1454864/1‑101 (MQ=255)
                                                                       aaGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGTGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTcc                               >  2:2311959/1‑101 (MQ=255)
                                                                        aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg                              >  1:789907/1‑101 (MQ=255)
                                                                          gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCgg                             >  1:656106/5‑101 (MQ=255)
                                                                          gatgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGGCTTTCTCAAGCGTTCCgg                             >  2:3614084/5‑101 (MQ=255)
                                                                            tgaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCt                           >  1:3107867/3‑101 (MQ=255)
                                                                             gaaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTc                          >  1:4622746/2‑101 (MQ=255)
                                                                              aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa                         >  2:2651670/1‑101 (MQ=255)
                                                                              aaaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCa                         >  2:3014232/1‑101 (MQ=255)
                                                                               aaGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGCCTTTCTCAAGCGTTCCGGCTCAg                        >  1:4593618/1‑101 (MQ=255)
                                                                                 gACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGaa                      >  2:669812/1‑101 (MQ=255)
                                                                                  aCCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAc                     >  1:1278268/1‑101 (MQ=255)
                                                                                   ccATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAAcc                    >  2:4425626/1‑101 (MQ=255)
                                                                                    cATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCg                   >  1:4443634/1‑101 (MQ=255)
                                                                                     aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg                  >  1:3227570/1‑101 (MQ=255)
                                                                                     aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg                  >  1:1478860/1‑101 (MQ=255)
                                                                                     aTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCgg                  <  1:3444698/101‑1 (MQ=255)
                                                                                      tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa                 >  2:418448/1‑101 (MQ=255)
                                                                                      tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa                 >  1:4338308/1‑101 (MQ=255)
                                                                                      tGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGa                 <  1:2861069/101‑1 (MQ=255)
                                                                                       gggTTCATCTTGGCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGaa                <  1:4251430/101‑1 (MQ=255)
                                                                                       gggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGaa                <  1:13629/101‑1 (MQ=255)
                                                                                        ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg               <  2:3613235/101‑1 (MQ=255)
                                                                                        ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg               >  1:165271/1‑101 (MQ=255)
                                                                                        ggTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAg               >  1:4221203/1‑101 (MQ=255)
                                                                                           tCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGccc            <  2:1476632/101‑1 (MQ=255)
                                                                                             aTCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgc          >  2:3746194/1‑101 (MQ=255)
                                                                                              tCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcg         >  1:4326358/1‑101 (MQ=255)
                                                                                              tCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAAAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcggg         >  1:2382846/1‑99 (MQ=255)
                                                                                              tCTGGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGTCGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcg         <  2:1498101/101‑1 (MQ=255)
                                                                                               cTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcgc        <  1:1541406/101‑1 (MQ=255)
                                                                                                ttGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCcgcgcg       >  2:3468863/1‑101 (MQ=255)
                                                                                                 tGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCgcgcgc      >  2:3150588/1‑101 (MQ=255)
                                                                                                    cTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCAcc   >  1:4185629/1‑101 (MQ=255)
                                                                                                     tCAAATACCCCGGGCGGAGTCTTGAAAGGAGGGGGGGCAGACCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCACCt  <  2:3107623/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
GCGCAGGCAAAAGGGGGGCGCTGCCCCCCTCTT.GG.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCGGCTCAGAACCGGAAGCCCGCGCGCACCT  >  NC_014034/2640126‑2640325

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG