Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,715,979 A→G 100% F8L (TTC→CTC)  amaB ← N‑carbamoyl‑L‑amino acid amidohydrolase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,715,9790A→G100.0% 778.4 206F8L (TTC→CTC) amaBN‑carbamoyl‑L‑amino acid amidohydrolase
Reads supporting (aligned to +/- strand):  new base (101/105):  ref base (0/0):  total (101/105)

Only 100 of 216 total aligned reads displayed.

ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076
                                                                                                  |                                                                                                 
atcggTCAGGGTTTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 <  1:4555794/101‑1 (MQ=255)
atcggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGt                                                                                                 >  2:964542/1‑101 (MQ=255)
  cggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGttt                                                                                               <  2:3694190/101‑1 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              <  1:3376262/101‑1 (MQ=255)
   ggTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGtttt                                                                                              <  2:2745978/101‑1 (MQ=255)
    gTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTtc                                                                                             >  2:1810251/1‑101 (MQ=255)
     tCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGATTTTGCACGGAGGTTTtct                                                                                            >  2:3072756/1‑101 (MQ=255)
         ggTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGCGGTTTTCTCCgg                                                                                        <  1:2525543/101‑1 (MQ=255)
           tCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      <  1:3388285/101‑1 (MQ=255)
           tCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      >  1:1575487/1‑101 (MQ=255)
           tCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      <  2:3117939/101‑1 (MQ=255)
           tCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      >  1:4599281/1‑101 (MQ=255)
           aagggCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGt                                                                                      >  2:3879813/4‑101 (MQ=255)
            cTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTg                                                                                     >  1:1480949/1‑101 (MQ=255)
             tGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGa                                                                                    >  2:639017/1‑101 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCCTGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 >  1:855209/1‑101 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 >  1:3396531/1‑101 (MQ=255)
                cGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATgg                                                                                 <  2:3002099/101‑1 (MQ=255)
                  gttgttGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCa                                                                               <  2:731236/101‑1 (MQ=255)
                   ttgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCAt                                                                              >  2:518316/1‑101 (MQ=255)
                    tgttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATc                                                                             <  2:4364848/101‑1 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            >  1:4481716/1‑101 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            >  1:4277628/1‑101 (MQ=255)
                     gttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCt                                                                            <  1:100169/101‑1 (MQ=255)
                      ttgCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTg                                                                           <  1:3963768/101‑1 (MQ=255)
                       tgcgtcccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          >  2:3949090/6‑101 (MQ=255)
                       cttccgatctgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTgg                                                                          <  1:2395049/91‑1 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         <  2:2293564/101‑1 (MQ=255)
                        gCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGc                                                                         <  1:1130243/101‑1 (MQ=255)
                           ccccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGt                                                                      <  1:1048674/101‑1 (MQ=255)
                            cccGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGtc                                                                     <  2:1371305/101‑1 (MQ=255)
                                cgATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTccc                                                                 >  1:536331/1‑101 (MQ=255)
                                  aTCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGCGATGGCATCTGGCTGTCTCCCTg                                                               >  2:1297060/1‑101 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              <  2:1768759/101‑1 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              >  1:482268/1‑101 (MQ=255)
                                   tCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTATCTCCGGGTGATGGCATCTGGCTGTCTCCCTgt                                                              >  1:3978673/1‑101 (MQ=255)
                                     cccGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTgtcg                                                            <  1:2818261/101‑1 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  2:815411/1‑101 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         >  1:2002988/1‑101 (MQ=255)
                                        ggCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCt                                                         <  2:4598981/101‑1 (MQ=255)
                                         gCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTg                                                        <  1:2773983/101‑1 (MQ=255)
                                          cccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGc                                                       >  2:4286859/1‑101 (MQ=255)
                                           ccGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCg                                                      <  1:67773/101‑1 (MQ=255)
                                              aCCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCgggg                                                   <  2:2582342/101‑1 (MQ=255)
                                              aCCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCgggg                                                   >  2:3421976/1‑101 (MQ=255)
                                              aCCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCgggg                                                   >  1:796236/1‑101 (MQ=255)
                                               ccTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGc                                                  <  2:1078247/101‑1 (MQ=255)
                                                cTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCt                                                 <  2:3590398/101‑1 (MQ=255)
                                                cTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCt                                                 >  1:1867314/1‑101 (MQ=255)
                                                 tGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCtt                                                >  1:954657/1‑101 (MQ=255)
                                                  gcgcCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCttt                                               <  2:4488694/101‑1 (MQ=255)
                                                      cATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGccc                                           >  1:1394871/1‑101 (MQ=255)
                                                      cATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGccc                                           <  1:972559/101‑1 (MQ=255)
                                                       aTCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCct                                          >  2:2793395/1‑101 (MQ=255)
                                                        tCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctc                                         >  1:4064433/1‑101 (MQ=255)
                                                         cTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctct                                        >  1:1694123/1‑101 (MQ=255)
                                                         cTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCctct                                        <  1:2508332/101‑1 (MQ=255)
                                                          tGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCtt                                       >  1:4350298/1‑101 (MQ=255)
                                                          tGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCtt                                       >  1:1150486/1‑101 (MQ=255)
                                                          tGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCtt                                       <  1:358857/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      <  2:3725057/101‑1 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      >  2:4225367/1‑101 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      >  2:1948796/1‑101 (MQ=255)
                                                           gCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTg                                      >  1:3152456/1‑101 (MQ=255)
                                                            cATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGc                                     <  2:2688843/101‑1 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  1:1266550/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  2:2207204/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  2:2438798/1‑101 (MQ=255)
                                                               gAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGAc                                  >  2:322841/1‑101 (MQ=255)
                                                                 ggCTGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTc                                <  1:1887755/101‑1 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             >  2:4234984/1‑101 (MQ=255)
                                                                    tGTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGGGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtt                             >  1:2056423/1‑101 (MQ=255)
                                                                     gTCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAttt                            <  2:451548/101‑1 (MQ=255)
                                                                      tCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtttt                           <  2:1869598/101‑1 (MQ=255)
                                                                      tCCCACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCAtttt                           <  1:1279688/101‑1 (MQ=255)
                                                                        ccACAGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCt                         <  1:3880098/101‑1 (MQ=255)
                                                                         cacaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCCGGCTGTTTCCCTGTCGTCTGCGGGGCTTTGCCCGCCTGCGCCTCATGTTCTg                        >  2:1490194/1‑101 (MQ=255)
                                                                          acaGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGa                       >  2:4078794/1‑101 (MQ=255)
                                                                           caggaccgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAc                      >  2:943052/7‑101 (MQ=255)
                                                                           caGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAc                      >  1:1772489/1‑101 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     <  2:560998/101‑1 (MQ=255)
                                                                            aGGCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGAcc                     <  1:3744532/101‑1 (MQ=255)
                                                                             ggCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCg                    <  1:3605247/101‑1 (MQ=255)
                                                                             ggCGCGCGGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCg                    <  2:1684535/101‑1 (MQ=255)
                                                                               cgcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAt                  >  2:2342128/1‑101 (MQ=255)
                                                                                gcgcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGAtt                 <  2:1349299/101‑1 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               <  2:1150394/101‑1 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               >  2:4464251/1‑101 (MQ=255)
                                                                                  gcgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTgg               >  2:1969793/1‑101 (MQ=255)
                                                                                   cgGAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGt              >  1:3716870/1‑101 (MQ=255)
                                                                                    ggAATTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTc             <  2:2169215/101‑1 (MQ=255)
                                                                                       aTTGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCaga          >  1:776716/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCTACTCATTTTCTGACCGATTGGTCAGAgg        >  1:3083464/1‑101 (MQ=255)
                                                                                         tGATACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        <  1:4225655/101‑1 (MQ=255)
                                                                                         tGATACGGAGGTTTGCTCCGGGTGATGGCATCTGGCTGGCTCCCTGTCGTGTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAgg        <  1:2176747/101‑1 (MQ=255)
                                                                                           aTACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaa      >  2:3746934/1‑101 (MQ=255)
                                                                                           aTACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaa      >  2:2128959/1‑101 (MQ=255)
                                                                                            tACGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGaaa     <  2:1266457/101‑1 (MQ=255)
                                                                                              cGGAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAaga   <  1:2291707/101‑1 (MQ=255)
                                                                                               ggAGGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAagag  >  1:2154022/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGGTCACGGTCTGGCGGTTGTTGCCCCCCGCGATCCCCGGCCCGACCTGCGCCATCTGCATGAGGCTGTCCCACAGGCGCGCGGAATTGATACGGAAGTTTTCTCCGGGTGATGGCATCTGGCTGTCTCCCTGTCGTCTGCGGGGCTTTGCCCTCTTGCGACTCATTTTCTGACCGATTGGTCAGAGGAAAGAG  >  NC_014034/2715881‑2716076

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG