Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,729,707 G→C 100% H240Q (CAC→CAG RCAP_rcc02539 ← diguanylate cyclase/phosphodiesterase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,729,7070G→C100.0% 810.7 212H240Q (CAC→CAGRCAP_rcc02539diguanylate cyclase/phosphodiesterase
Reads supporting (aligned to +/- strand):  new base (128/84):  ref base (0/0):  total (128/84)

Only 100 of 223 total aligned reads displayed.

CGGGCGATTTCGGTCAGCAATTCATCGCCGAAGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCGTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATATTGGCCAGGA  >  NC_014034/2729609‑2729804
                                                                                                  |                                                                                                 
 gggcgATTTCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCctgt                                                                                                >  1:2224631/1‑101 (MQ=255)
   gcgATTTCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTcc                                                                                              <  1:4338345/101‑1 (MQ=255)
   gcgATTTCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTcc                                                                                              <  2:1386670/101‑1 (MQ=255)
    cgATTTCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCGTCAAGAGCAGCGCAAAGCCCTGTCCg                                                                                             >  1:2803525/1‑101 (MQ=255)
     gATTTCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGc                                                                                            >  2:4223911/1‑101 (MQ=255)
      aTTTCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCa                                                                                           >  2:2974657/1‑101 (MQ=255)
        ttCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCTGCGCAAAGCCCTGTCCGCAGc                                                                                         <  1:2643912/101‑1 (MQ=255)
        ttCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGc                                                                                         >  2:4405533/1‑101 (MQ=255)
         tCGGTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGcc                                                                                        >  2:2783897/1‑101 (MQ=255)
           ggTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAACGCCCTGTCCGCAGCcgc                                                                                      >  2:3428760/1‑101 (MQ=255)
           ggTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCcgc                                                                                      <  1:3607462/101‑1 (MQ=255)
            gTCAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCcgcg                                                                                     >  1:4565722/1‑101 (MQ=255)
              cAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCt                                                                                   <  1:1251825/101‑1 (MQ=255)
              cAGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCt                                                                                   >  1:2577032/1‑101 (MQ=255)
               aGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTg                                                                                  <  1:2936949/101‑1 (MQ=255)
               aGCAATTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTg                                                                                  <  2:2064327/101‑1 (MQ=255)
                   aTTCATCGCCGAGGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCgg                                                                              <  1:3883241/101‑1 (MQ=255)
                   aTTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCgg                                                                              <  1:376327/101‑1 (MQ=255)
                   aTTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCgg                                                                              <  1:3223577/101‑1 (MQ=255)
                   aTTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCgg                                                                              <  2:534295/101‑1 (MQ=255)
                   aTTCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCgg                                                                              >  1:4312058/1‑101 (MQ=255)
                    ttCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCggg                                                                             <  1:2247362/101‑1 (MQ=255)
                    ttCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCggg                                                                             <  1:1725786/101‑1 (MQ=255)
                     tCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGc                                                                            >  2:1162285/1‑101 (MQ=255)
                     tCATCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGc                                                                            >  1:1043973/1‑101 (MQ=255)
                       aTCGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCa                                                                          <  1:4251715/101‑1 (MQ=255)
                         cGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCCcc                                                                        >  2:3611249/1‑101 (MQ=255)
                         cGCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCAcc                                                                        >  2:2453492/1‑101 (MQ=255)
                          gCCGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCg                                                                       >  1:3352857/1‑101 (MQ=255)
                            cGACGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGcc                                                                     >  1:3456931/1‑101 (MQ=255)
                              acgCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCATc                                                                   <  2:4076184/99‑1 (MQ=255)
                               cgCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCg                                                                  <  2:2092306/100‑1 (MQ=255)
                                gCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGcccccg                                                                 >  1:3050654/1‑97 (MQ=255)
                                  cATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCCCCGCCTCGGcc                                                               >  2:531884/1‑101 (MQ=255)
                                  cATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGcc                                                               >  1:2867871/1‑101 (MQ=255)
                                  cATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGcc                                                               >  2:2364490/1‑101 (MQ=255)
                                  cATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGATGGCGGGCCCACGCCTCGGca                                                               >  2:2189698/1‑100 (MQ=255)
                                   aTGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCa                                                              <  1:3711495/101‑1 (MQ=255)
                                        cGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAggcgg                                                         >  2:1562486/1‑101 (MQ=255)
                                         gAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGGGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCCCGGGCAggcggg                                                        >  1:1441138/1‑101 (MQ=255)
                                         gAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAggcggg                                                        >  1:860459/1‑101 (MQ=255)
                                         gAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAggcggg                                                        >  1:579430/1‑101 (MQ=255)
                                          aaCAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAggcgggg                                                       >  1:4525781/1‑100 (MQ=255)
                                           aCAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAggcgggcg                                                      >  2:1885526/1‑101 (MQ=255)
                                            cAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGt                                                     <  2:339184/101‑1 (MQ=255)
                                            cAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGt                                                     >  1:1564764/1‑101 (MQ=255)
                                             aGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAggcgggcggt                                                    >  2:4364248/1‑99 (MQ=255)
                                             aGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGtt                                                    >  2:3876177/1‑101 (MQ=255)
                                              ggTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTg                                                   >  2:1285878/1‑101 (MQ=255)
                                              ggTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTg                                                   <  2:4479913/101‑1 (MQ=255)
                                                  gTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAgg                                               >  1:728643/1‑101 (MQ=255)
                                                   ttGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGc                                              >  1:4640166/1‑101 (MQ=255)
                                                    tGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCCGCCAGGCGGGCGTTGAAGGcc                                             >  2:3213176/1‑101 (MQ=255)
                                                     gACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCt                                            <  1:361829/101‑1 (MQ=255)
                                                     gACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCt                                            >  1:165795/1‑101 (MQ=255)
                                                     gACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCt                                            <  1:1074627/101‑1 (MQ=255)
                                                         ttCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCg                                        <  1:2393771/101‑1 (MQ=255)
                                                         ttCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCg                                        >  2:841700/1‑101 (MQ=255)
                                                          tCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCgg                                       <  2:899077/101‑1 (MQ=255)
                                                          tCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGGTGAAGGCCTTGCgg                                       >  1:153710/1‑101 (MQ=255)
                                                            ttGAACCGGTCGAGATCCATCAAGAGCAGCGCACAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGtt                                     <  1:3977692/101‑1 (MQ=255)
                                                            ttGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGtt                                     >  2:2254313/1‑101 (MQ=255)
                                                             tGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCCCCCCCTCCGCCAGGCGGGCGGTGAAGGCCTCGCGGTTc                                    >  1:1021689/1‑101 (MQ=255)
                                                             tGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGGTc                                    >  2:4637627/1‑101 (MQ=255)
                                                              gAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTca                                   <  1:3169477/101‑1 (MQ=255)
                                                              gAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTca                                   >  1:791699/1‑101 (MQ=255)
                                                              gAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGGTca                                   >  2:309796/1‑101 (MQ=255)
                                                               aaCCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTcag                                  <  1:1220843/101‑1 (MQ=255)
                                                                aCCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTcagc                                 <  2:1352242/101‑1 (MQ=255)
                                                                 ccGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCGGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTcagca                                <  1:920517/101‑1 (MQ=255)
                                                                  cGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTcagcag                               <  1:4380386/101‑1 (MQ=255)
                                                                  cGGTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGGTccgcag                               >  2:684884/1‑101 (MQ=255)
                                                                    gTCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGcc                             <  1:4364540/101‑1 (MQ=255)
                                                                     tCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGccc                            <  1:2784095/101‑1 (MQ=255)
                                                                     tCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGccc                            <  1:3153959/101‑1 (MQ=255)
                                                                     tCGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCACGCGGGCGTTGAAGGCCTTGCGGGTCAGCAGccc                            >  1:4016210/1‑101 (MQ=255)
                                                                      cGAGATCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCg                           >  1:2832331/1‑101 (MQ=255)
                                                                       gagaTCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCgg                          >  1:365209/1‑101 (MQ=255)
                                                                        agaTCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGccccgg                         >  2:2576351/1‑98 (MQ=255)
                                                                        agaTCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCggt                         <  1:4185149/101‑1 (MQ=255)
                                                                        agaTCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCggg                         >  2:4572247/1‑100 (MQ=255)
                                                                         gaTCCATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCggtg                        <  2:1179999/101‑1 (MQ=255)
                                                                            ccATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGgtggtg                     <  2:1256433/101‑1 (MQ=255)
                                                                             cATCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGgtgt                    <  2:3931234/101‑1 (MQ=255)
                                                                              aTCAAGAGCAGCGCAAGGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTgtc                   <  1:552746/101‑1 (MQ=255)
                                                                               tCAAGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCCGTGGTgtcg                  >  2:381525/1‑101 (MQ=255)
                                                                                 aaGAGCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTgtcgtc                >  2:4178530/1‑101 (MQ=255)
                                                                                  agagCAGCGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGtcgtcg               <  2:1657952/101‑1 (MQ=255)
                                                                                    agcagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCCGCCCGGGGGTGTCGTCGat             >  1:245666/1‑101 (MQ=255)
                                                                                     gcagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGGGTCGTCGata            >  1:1835509/1‑101 (MQ=255)
                                                                                      cagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGatat           <  2:3619312/101‑1 (MQ=255)
                                                                                      cagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGatat           >  2:3041138/1‑101 (MQ=255)
                                                                                      cagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGGGTCGTCGatat           >  2:1635428/1‑101 (MQ=255)
                                                                                      cagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGGGGTGTCGTCGatat           >  1:1900774/1‑101 (MQ=255)
                                                                                      cagcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGGGGTGTCGTCGatat           >  1:3034623/1‑101 (MQ=255)
                                                                                       agcGCAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATAtt          <  2:790043/101‑1 (MQ=255)
                                                                                        gcgcAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATATTg         <  2:2318468/101‑1 (MQ=255)
                                                                                         cgcAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATATTgg        <  2:1515107/101‑1 (MQ=255)
                                                                                          gcAAAGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATATTGGc       <  1:4495268/101‑1 (MQ=255)
                                                                                              aGCCCTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATATTGGCCAgg   <  1:981531/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CGGGCGATTTCGGTCAGCAATTCATCGCCGAAGCCATGGCCGAACAGGTCGTTGACATTCTTGAACCGGTCGAGATCCATCAAGAGCAGCGCAAAGCCGTGTCCGCAGCCGCGCTGGCGGGCCACCGCCTCGGCCAGGCGGGCGTTGAAGGCCTTGCGGTTCAGCAGCCCGGTGGTGTCGTCGATATTGGCCAGGA  >  NC_014034/2729609‑2729804

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG