Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,854	C→G	100%	intergenic (‑186/+107)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,854	0	C→G	100.0%	87.7	31	intergenic (‑186/+107)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (12/19):  ref base (0/0):  total (12/19)

AATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747759‑2747950                                                                                                   |                                                                                                  aaggggAACCGCCCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgag                                                                                               <  1:3807802/97‑1 (MQ=25)  atgggAAACGCGCCCCTCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgaga                                                                                              <  1:1742254/98‑1 (MQ=25)   aaGGAAACGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGaa                                                                                             <  1:1195940/100‑1 (MQ=37)    agaaaaCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAg                                                                                            <  1:3342036/98‑1 (MQ=25)         gcgcgCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                       <  1:4355321/100‑1 (MQ=25)         ccccgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                       <  2:2793087/95‑1 (MQ=25)         aCGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                       <  2:3319303/101‑1 (MQ=25)         aCGCCACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                       <  2:4642367/101‑1 (MQ=25)          cgcgACCCGCGTTT.CCCCGACCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccg                                                                                      <  1:1432911/101‑1 (MQ=25)           gcgCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccagg                                                                                     <  2:3160504/101‑4 (MQ=21)            gcgcccCCGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgc                                                                                     <  2:4071989/98‑1 (MQ=21)            ccccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgcc                                                                                    <  2:3400290/98‑1 (MQ=25)              ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                                 <  1:2099466/100‑1 (MQ=25)                ccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggc                                                                               <  2:19395/101‑3 (MQ=25)                 cgcgTGC.CCCCCCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  1:3348533/101‑4 (MQ=21)                 cgcgTCC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  1:2743619/101‑4 (MQ=21)                  ccttctcccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCGGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                              <  1:2618177/94‑4 (MQ=21)                    ggcccccccGCCCGCAGCGCCCGGTTGCGGCATGGCCCCTTCAGCCCGCACCACAGAGGGCAGCCCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                            <  1:3540001/96‑6 (MQ=21)                             gcccgcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGccc                                                                    >  1:2730801/1‑101 (MQ=21)                                 gcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGCgg                                                                >  2:17465/1‑101 (MQ=21)                                 cccgcgcCCGGTGCCGGCATGGCGGCTTCAGCCCGCACCACAGAGGGCAGCGCTGGGCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAgg                                                                <  2:201514/98‑1 (MQ=12)                                                                                       cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGCCACAGGACCTCTGTGACGCATGGcagaacggaag          >  1:748448/1‑91 (MQ=17)                                                                                        ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCCTGGTCAAGGCGAtcg         >  2:1929585/1‑99 (MQ=18)                                                                                        ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg         >  1:2973239/1‑101 (MQ=18)                                                                                          gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc        >  1:2874694/6‑101 (MQ=18)                                                                                          gcccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGc        >  2:3444205/6‑101 (MQ=18)                                                                                            ccggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCt      >  2:1544804/4‑101 (MQ=21)                                                                                             cggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCtt     >  1:4234037/3‑101 (MQ=21)                                                                                              ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg    >  1:254778/2‑101 (MQ=21)                                                                                              ggcgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTg    >  2:789648/2‑101 (MQ=21)                                                                                                cgGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGc  >  1:3710024/1‑101 (MQ=21)                                                                                                   |                                                                                                  AATAGGAAACGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGGCCTTGGC  >  NC_014034/2747759‑2747950 Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG