Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,770,015 C→G 100% H311Q (CAC→CAG RCAP_rcc02581 → sensor histidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,770,0150C→G100.0% 802.2 211H311Q (CAC→CAGRCAP_rcc02581sensor histidine kinase
Reads supporting (aligned to +/- strand):  new base (104/107):  ref base (0/0):  total (104/107)

Only 100 of 218 total aligned reads displayed.

GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112
                                                                                                  |                                                                                                 
   aTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGGGATCTGCGCCTTTGTCGCGCAGGTGGt                                                                                              >  2:3523282/1‑101 (MQ=255)
   aTGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGt                                                                                              >  1:3806511/1‑101 (MQ=255)
    tGGCCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTc                                                                                             >  2:3955379/1‑101 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            >  1:778929/1‑101 (MQ=255)
     gggCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCg                                                                                            >  1:2015605/1‑101 (MQ=255)
       gCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGaa                                                                                          <  1:3492160/101‑1 (MQ=255)
       gCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGaa                                                                                          <  1:4107596/101‑1 (MQ=255)
        cAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAg                                                                                         <  1:4061737/101‑1 (MQ=255)
        cAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAg                                                                                         >  2:1971963/1‑101 (MQ=255)
        cAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAg                                                                                         >  2:3693529/1‑101 (MQ=255)
         gaGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        <  2:2208293/100‑1 (MQ=255)
         aaGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGa                                                                                        >  1:176131/1‑101 (MQ=255)
          aGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGAt                                                                                       >  2:2424511/1‑101 (MQ=255)
           gatgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATg                                                                                      <  2:2193639/101‑1 (MQ=255)
           gatgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATg                                                                                      <  1:3235443/101‑1 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     <  1:2683413/101‑1 (MQ=255)
            atgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGc                                                                                     >  1:3539459/1‑101 (MQ=255)
             tgatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGcc                                                                                    >  1:97072/1‑101 (MQ=255)
              gatCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGGGGAAATCTGCGCCTTTTTCGCGCGGGTGGCCCAAGAGGCCg                                                                                   >  2:4408469/1‑101 (MQ=255)
               atCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGt                                                                                  <  1:1939397/101‑1 (MQ=255)
                tCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGCGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTg                                                                                 <  1:3922064/101‑1 (MQ=255)
                tCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTg                                                                                 <  1:4346029/101‑1 (MQ=255)
                 cGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgc                                                                                <  1:21417/101‑1 (MQ=255)
                  gACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcg                                                                               <  2:1562106/101‑1 (MQ=255)
                     gCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTgcgcgc                                                                            >  1:3041116/1‑101 (MQ=255)
                        ttCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCgg                                                                         <  2:2977944/101‑1 (MQ=255)
                         tCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGccccc                                                                        <  1:842667/101‑4 (MQ=255)
                         tCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCggg                                                                        >  2:1486597/1‑101 (MQ=255)
                          ccTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGc                                                                       >  2:3469297/1‑101 (MQ=255)
                          ccTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGc                                                                       <  1:561867/101‑1 (MQ=255)
                             gAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGc                                                                    >  1:65229/1‑101 (MQ=255)
                             gAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGc                                                                    >  1:2405424/1‑101 (MQ=255)
                                tGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCgg                                                                 >  2:10625/1‑101 (MQ=255)
                                  tCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTg                                                               <  2:3806226/101‑1 (MQ=255)
                                   cgggcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              <  2:2041826/98‑1 (MQ=255)
                                   cgcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGTGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              <  1:1542100/101‑1 (MQ=255)
                                   cgcgcgcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGc                                                              <  2:1014004/101‑1 (MQ=255)
                                        gcgAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtg                                                         <  2:1632720/101‑1 (MQ=255)
                                        gagAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtg                                                         <  2:3641326/99‑1 (MQ=255)
                                         ggAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCtgg                                                        <  1:3442785/100‑1 (MQ=255)
                                             gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    >  2:827686/1‑101 (MQ=255)
                                             gagGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGc                                                    >  1:1584434/1‑101 (MQ=255)
                                                gCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTg                                                 <  2:2504103/101‑1 (MQ=255)
                                                 cGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGc                                                >  1:3668321/1‑101 (MQ=255)
                                                  gATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcc                                               >  2:1994400/1‑101 (MQ=255)
                                                  gATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGcc                                               >  2:2220287/1‑101 (MQ=255)
                                                   aTGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGccc                                              >  1:3073206/1‑101 (MQ=255)
                                                     ggcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCg                                            >  1:2496623/1‑101 (MQ=255)
                                                     ggcgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCg                                            <  2:564447/101‑1 (MQ=255)
                                                       cgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          <  2:3072962/101‑1 (MQ=255)
                                                       cgggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGAt                                          <  2:4067589/101‑1 (MQ=255)
                                                        gggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATg                                         <  2:392318/101‑1 (MQ=255)
                                                         ggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgc                                        <  1:813983/101‑1 (MQ=255)
                                                         ggcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgc                                        >  1:1482576/1‑101 (MQ=255)
                                                          gcgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcg                                       <  1:3641716/101‑1 (MQ=255)
                                                           cgAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATgcgc                                      <  1:185032/101‑1 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     >  1:4533542/1‑101 (MQ=255)
                                                            gAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGcc                                     >  1:4446788/1‑101 (MQ=255)
                                                             aGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCg                                    <  2:1697212/101‑1 (MQ=255)
                                                             aGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCg                                    <  1:1059890/101‑1 (MQ=255)
                                                                 cGAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGc                                >  1:1359540/1‑101 (MQ=255)
                                                                 cGAACGGGTGGAGATCTGCGCCTTTGTCGAGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGc                                >  2:3550992/1‑101 (MQ=255)
                                                                  gAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCa                               >  1:1322802/1‑101 (MQ=255)
                                                                  gAACGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCa                               <  2:1123934/101‑1 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  2:1271843/1‑101 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  1:1772103/1‑101 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              <  1:2863228/101‑1 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              <  1:1151734/101‑1 (MQ=255)
                                                                   aaCGGGTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              >  1:3292310/1‑101 (MQ=255)
                                                                   aaCGGGTGGAAATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAg                              <  1:1972691/101‑1 (MQ=255)
                                                                      gggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCg                           >  2:3012586/1‑101 (MQ=255)
                                                                      gggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCg                           >  2:2740018/1‑101 (MQ=255)
                                                                      gggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCg                           <  2:1406465/101‑1 (MQ=255)
                                                                       ggTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCgg                          >  2:4540206/1‑101 (MQ=255)
                                                                        gTGGAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGt                         <  2:778854/101‑1 (MQ=255)
                                                                          ggAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       <  2:65223/101‑1 (MQ=255)
                                                                          ggAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       >  1:4441918/1‑101 (MQ=255)
                                                                          ggAGATCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGCTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgc                       >  1:3974104/1‑101 (MQ=255)
                                                                           gagaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgct                      <  2:1883685/101‑1 (MQ=255)
                                                                            agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     <  2:4004869/101‑1 (MQ=255)
                                                                            agaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctg                     <  1:1765373/101‑1 (MQ=255)
                                                                             gaTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGtgctgc                    <  2:4319582/101‑1 (MQ=255)
                                                                              aTCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcg                   >  1:4308534/1‑101 (MQ=255)
                                                                               tCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcgc                  >  2:3225631/1‑101 (MQ=255)
                                                                               tCTGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTgcgc                  <  1:524621/101‑1 (MQ=255)
                                                                                 tGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGccc                <  1:457966/101‑1 (MQ=255)
                                                                                 tGCGCCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGccc                >  1:2988863/1‑101 (MQ=255)
                                                                                  gcgcCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGcccc               >  2:2365363/1‑101 (MQ=255)
                                                                                   cgcCTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCg              <  1:1381986/101‑1 (MQ=255)
                                                                                     ccTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGGGCTGGGCCCCGag            >  2:4256568/1‑100 (MQ=255)
                                                                                      cTTTGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtg           <  2:2074268/101‑1 (MQ=255)
                                                                                       tttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgc          <  2:900713/101‑1 (MQ=255)
                                                                                       tttGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgc          <  1:2424675/101‑1 (MQ=255)
                                                                                         tGTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgc        >  1:440870/1‑101 (MQ=255)
                                                                                          gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGTCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGGGCTGCGCCCCGAtgcgct       >  1:4160346/1‑101 (MQ=255)
                                                                                          gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       <  2:4181694/101‑1 (MQ=255)
                                                                                          gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       <  1:1180634/101‑1 (MQ=255)
                                                                                          gTCGCGCAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgct       >  2:1835275/1‑101 (MQ=255)
                                                                                             gcgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcg    >  2:3273369/1‑101 (MQ=255)
                                                                                              cgcAGGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGAtgcgctgcgc   >  2:104517/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GAGATGGGCAAGATGATCGACGCCTTCCTGAATGTCGCGCGCGAAGAGGCGATGGCGGGCGAGCCCGAACGGGTGGAGATCTGCGCCTTTGTCGCGCACGTGGTCGAAGATGCCGTGCGCGCCGGGCAGCCGGTGCAGCTGGTGGCCTGCCCCGATGCGCCGGTGCAGGCGGTGCTGCGCCCCGATGCGCTGCGCC  >  NC_014034/2769917‑2770112

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG