Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,808,441 C→T 100% P352L (CCC→CTC)  pyrD2 → dihydroorotate oxidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,808,4410C→T100.0% 807.4 210P352L (CCC→CTC) pyrD2dihydroorotate oxidase
Reads supporting (aligned to +/- strand):  new base (92/118):  ref base (0/0):  total (92/118)

Only 100 of 216 total aligned reads displayed.

TCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCG  >  NC_014034/2808343‑2808538
                                                                                                  |                                                                                                   
  tGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGa                                                                                                 >  1:165746/1‑101 (MQ=255)
  tGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGa                                                                                                 >  2:1821628/1‑101 (MQ=255)
     cggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAccc                                                                                              <  1:2025405/101‑1 (MQ=255)
      ggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccg                                                                                             >  2:1678984/1‑101 (MQ=255)
      ggcCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccg                                                                                             >  2:2402731/1‑101 (MQ=255)
        ccGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcc                                                                                           >  2:1355228/1‑101 (MQ=255)
        ccGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcc                                                                                           <  1:4472847/101‑1 (MQ=255)
        ccGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcc                                                                                           >  2:812183/1‑101 (MQ=255)
        ccGAGGTCGCGCGCGGCCGGGATGCGCTTTGGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcc                                                                                           <  1:967957/101‑1 (MQ=255)
         cGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgcct                                                                                          <  2:1677792/101‑2 (MQ=255)
         cGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGAcccgccc                                                                                          >  1:2556941/1‑101 (MQ=255)
            ggTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTa                                                                                       >  1:2674773/1‑101 (MQ=255)
             gTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAg                                                                                      <  2:417036/101‑1 (MQ=255)
             gNNGCNCGCGGCCTGGANGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAg                                                                                      <  2:1040917/101‑1 (MQ=255)
              ccgcgcgcgGCCTGGATGCGCTTTTGACGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGc                                                                                     <  1:3916220/100‑1 (MQ=255)
               cgcccgcgGCCTGGGTGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCt                                                                                    <  1:1405308/101‑1 (MQ=255)
                gcgcgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTg                                                                                   <  2:552276/101‑1 (MQ=255)
                   cgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..gg                                                                              <  2:2664334/101‑1 (MQ=255)
                   cgcgGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..gg                                                                              >  1:3146156/1‑101 (MQ=255)
                      ggCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..ggggg                                                                           <  1:4415738/101‑1 (MQ=255)
                      ggCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..ggggg                                                                           >  2:1280163/1‑101 (MQ=255)
                        ccTGGATGCGCTTTTGGCGCGCGACGGTTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..ggggggt                                                                         >  1:2294968/1‑99 (MQ=255)
                        ccTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..ggggggt                                                                         >  2:4459534/1‑99 (MQ=255)
                        ccTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..cgggggg                                                                         >  2:4652380/1‑99 (MQ=255)
                         cTGGATGCGCTTTTGGGGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGGGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGGTGC..GGGGGgtt                                                                        >  2:1215390/1‑101 (MQ=255)
                         cTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..cggggggg                                                                        >  1:218516/1‑98 (MQ=255)
                         cTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGttt                                                                        >  1:703624/1‑101 (MQ=255)
                         cTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGgtt                                                                        >  1:362877/1‑101 (MQ=255)
                           ggATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTca                                                                      <  1:1520498/101‑1 (MQ=255)
                            gATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGGTTcac                                                                     >  2:3469378/1‑101 (MQ=255)
                              tGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCacac                                                                   <  1:869414/101‑1 (MQ=255)
                              tGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGATGC..GGGGGTTTCacac                                                                   <  2:1771778/101‑1 (MQ=255)
                               gcgcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcacc                                                                  <  2:3437645/101‑1 (MQ=255)
                                cgcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcaccc                                                                 <  2:3554937/101‑1 (MQ=255)
                                cgcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGATTCAcaccc                                                                 >  2:2329284/1‑101 (MQ=255)
                                 gcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGGTTCCcacccc                                                                >  1:2920676/1‑101 (MQ=255)
                                 gcTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..CGGGGTTTCAcacccc                                                                <  1:3931442/101‑1 (MQ=255)
                                  tttttGGCGCGCGACGTCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcaccccc                                                               <  2:39733/100‑1 (MQ=255)
                                  cTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcaccccc                                                               <  1:1817284/101‑1 (MQ=255)
                                  cTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcaccccc                                                               <  2:711464/101‑1 (MQ=255)
                                   ttttGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcacccccg                                                              <  2:4269830/101‑1 (MQ=255)
                                   ttttGGCGCGCGACGGCTTTGCTGCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCCGC..GGGGGTTTCAcacccccg                                                              <  2:1606531/101‑1 (MQ=255)
                                     ttGGCGCGGGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcacccccgca                                                            <  1:1089245/101‑1 (MQ=255)
                                           cgcgACGTCTTTTCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCAcacccccgcacccccg                                                      <  2:4318444/101‑1 (MQ=255)
                                            gcgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGCCGGGGGGGGTCAcaccccccccccccc                                                       >  2:115849/1‑101 (MQ=255)
                                             ggACGGCTTTGCTCCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTg                                                    <  1:3111426/100‑1 (MQ=255)
                                             cgACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTg                                                    >  1:1894953/1‑101 (MQ=255)
                                              gACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTgg                                                   <  2:889811/101‑1 (MQ=255)
                                              gACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTgg                                                   <  1:1961280/101‑1 (MQ=255)
                                              gACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGGTTCACACCCCCCCCCCCCCGTgg                                                   >  2:3284408/1‑101 (MQ=38)
                                               aCGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGc                                                  <  1:2888425/101‑1 (MQ=255)
                                                 ggCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGt                                                <  2:3233523/101‑1 (MQ=255)
                                                  gCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGGTTCACACCCCCGCACCCCCGTGGCGTa                                               >  2:1385894/1‑101 (MQ=255)
                                                   cTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAt                                              >  1:747996/1‑101 (MQ=255)
                                                    tttGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAtt                                             >  2:1171511/1‑101 (MQ=255)
                                                     ttGCTTCGGGCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTAttt                                            <  1:115863/101‑1 (MQ=255)
                                                      tGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTg                                           <  1:2402893/101‑1 (MQ=255)
                                                       gCTTCGGTCGGGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTgc                                          <  2:3361553/101‑1 (MQ=255)
                                                       gCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTgc                                          <  2:4553670/101‑1 (MQ=255)
                                                        cTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGATTCACACCCCCGCACCCCCGTGGCGTATTTgcg                                         >  1:4034161/1‑101 (MQ=255)
                                                          tggttgccgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCCCCCCCGTGGCGTATTTGCGcc                                       <  1:3845097/94‑1 (MQ=25)
                                                          tCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCGCACCCCCGCACCCCCGTGGCGTATTTGCGcc                                       <  1:4155312/101‑1 (MQ=255)
                                                           cGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCCCCCCCCTGGCGTATTTGCGccc                                      >  2:1788639/1‑100 (MQ=255)
                                                            ggTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCaa                                     <  1:4340343/101‑1 (MQ=255)
                                                            ggTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCaa                                     <  1:4549604/101‑1 (MQ=255)
                                                              tCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGa                                   <  1:4147157/101‑1 (MQ=255)
                                                              tCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGa                                   <  2:4414270/101‑1 (MQ=255)
                                                              tCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGa                                   <  1:2791883/101‑1 (MQ=255)
                                                               cgcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaa                                  <  2:2556767/101‑1 (MQ=255)
                                                                gcgGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaa                                 <  2:4055416/101‑1 (MQ=255)
                                                                  ggAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaaaa                               >  1:4665377/1‑101 (MQ=255)
                                                                  ggAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGaaaaa                               <  2:824290/101‑1 (MQ=255)
                                                                   gAAGCCGTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCTGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAg                              >  1:4045170/1‑101 (MQ=255)
                                                                        cGTGGGCCCCGGCCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAg                         <  1:957368/101‑1 (MQ=255)
                                                                         gTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCGCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGa                        >  2:4622285/1‑101 (MQ=255)
                                                                         gTGGGCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGa                        <  1:1836135/101‑1 (MQ=255)
                                                                           gggCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACt                      <  2:274719/101‑1 (MQ=255)
                                                                             gCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACttt                    >  2:588565/1‑101 (MQ=255)
                                                                             gCACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACttt                    <  1:124880/101‑1 (MQ=255)
                                                                              cACCGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACtttt                   >  1:48994/1‑101 (MQ=255)
                                                                               cccGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACGCCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTg                  <  2:4510770/100‑1 (MQ=255)
                                                                                 cGGTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGcc                >  2:2913524/1‑101 (MQ=255)
                                                                                  ggTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGccc               <  1:3469636/101‑1 (MQ=255)
                                                                                  ggTAGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGccc               <  2:3113593/101‑1 (MQ=255)
                                                                                   gTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCg              >  2:2620829/1‑101 (MQ=255)
                                                                                   gTCGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCg              <  2:2294806/101‑1 (MQ=255)
                                                                                     cGCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGtt            >  1:4301556/1‑101 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           <  1:2351258/101‑1 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           >  2:4572348/1‑101 (MQ=255)
                                                                                      gCAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTc           >  2:1069575/1‑101 (MQ=255)
                                                                                       cAGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCa          <  1:2564949/101‑1 (MQ=255)
                                                                                        aGCCGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCAc         <  1:625515/101‑1 (MQ=255)
                                                                                          ccGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCAccc       >  2:2017386/1‑101 (MQ=255)
                                                                                          ccGCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCAccc       <  1:4609202/101‑1 (MQ=255)
                                                                                            gCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTCTTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCAccccg     >  2:292996/1‑99 (MQ=255)
                                                                                            gCTGGCTCTGACCCGCCCGTAGCTGC..GGGGGGTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCCAGAAAAAGCACAGACTTTTGCCCCTTCACCCtt     >  1:1751963/1‑100 (MQ=14)
                                                                                             cTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTgg    >  2:1975900/1‑101 (MQ=255)
                                                                                             cTGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTgg    >  2:1106435/1‑101 (MQ=255)
                                                                                              tGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGgc   <  2:3667017/101‑1 (MQ=255)
                                                                                              tGGCTCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGgc   >  1:1061136/1‑101 (MQ=255)
                                                                                                  |                                                                                                   
TCTGGCGGCCGAGGTCGCGCGCGGCCTGGATGCGCTTTTGGCGCGCGACGGCTTTGCTTCGGTCGCGGAAGCCGTGGGCACCGGTCGCAGCCGCTGGCCCTGACCCGCCCGTAGCTGC..GGGGGTTTCACACCCCCGCACCCCCGTGGCGTATTTGCGCCAAGAAAAAGCACAGACTTTTGCCCGTTCACCCTGGCG  >  NC_014034/2808343‑2808538

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG