Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,925,566 A→C 100% intergenic (+350/‑85) RCAP_rcc02735 → / → RCAP_rcc02736 virulence‑associated protein E/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,925,5660A→C100.0% 1023.5 273intergenic (+350/‑85)RCAP_rcc02735/RCAP_rcc02736virulence‑associated protein E/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (127/145):  ref base (0/0):  total (128/145)

Only 100 of 275 total aligned reads displayed.

AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663
                                                                                                  |                                                                                                 
 ggTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCa                                                                                                >  2:4438514/1‑101 (MQ=255)
     ggACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCAtttt                                                                                            <  2:3936616/101‑1 (MQ=255)
       aCAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTcc                                                                                          >  1:1044610/1‑101 (MQ=255)
       aCAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTcc                                                                                          >  1:4340566/1‑101 (MQ=255)
       aCAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTcc                                                                                          >  1:4152795/1‑101 (MQ=255)
        cAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCt                                                                                         >  1:1173747/1‑101 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        >  2:655839/1‑101 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        >  1:3553163/1‑101 (MQ=255)
         aaGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCtt                                                                                        <  2:3252866/101‑1 (MQ=255)
           gCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGa                                                                                      <  2:921239/101‑1 (MQ=255)
           gCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGa                                                                                      >  1:3224293/1‑101 (MQ=255)
            cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGAt                                                                                     >  2:1068146/1‑101 (MQ=255)
            cATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGAt                                                                                     <  1:251078/101‑1 (MQ=255)
                 ttCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGt                                                                                <  1:4438809/101‑1 (MQ=255)
                 ttCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGt                                                                                >  1:959849/1‑101 (MQ=255)
                     gAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCt                                                                            <  2:3502898/101‑1 (MQ=255)
                      aaaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTg                                                                           <  1:2938879/101‑1 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          <  2:4406257/101‑1 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          >  2:2842232/1‑101 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          >  1:3064739/1‑101 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          <  1:2128372/101‑1 (MQ=255)
                       aaGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGc                                                                          <  2:454272/101‑1 (MQ=255)
                        aGGAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCa                                                                         >  1:2411312/1‑101 (MQ=255)
                         ggAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCaa                                                                        <  2:2605936/101‑1 (MQ=255)
                          gAAAATTTGGCGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAAc                                                                       <  2:3823504/101‑1 (MQ=255)
                          gAAAATTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAAc                                                                       <  2:3064495/101‑1 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     >  2:2252704/1‑101 (MQ=255)
                            aaaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGt                                                                     <  2:2454902/101‑1 (MQ=255)
                             aaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTg                                                                    <  2:3101645/101‑1 (MQ=255)
                             aaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTg                                                                    <  2:290503/101‑1 (MQ=255)
                             aaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTg                                                                    <  1:2120372/101‑1 (MQ=255)
                             aaTTTGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTg                                                                    >  2:2372871/1‑101 (MQ=255)
                               tttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAt                                                                  >  1:2723627/1‑101 (MQ=255)
                               tttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAt                                                                  >  1:2593496/1‑101 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 <  1:3858033/101‑1 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 >  2:4092148/1‑101 (MQ=255)
                                ttGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGAtt                                                                 >  1:4288266/1‑101 (MQ=255)
                                 tGACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTc                                                                <  2:3565864/101‑1 (MQ=255)
                                  gACGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTcc                                                               >  1:1706898/1‑101 (MQ=255)
                                   aCGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTccc                                                              <  1:3022025/101‑1 (MQ=255)
                                   aCGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTccc                                                              >  2:2125629/1‑101 (MQ=255)
                                   aCGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTccc                                                              <  1:420978/101‑1 (MQ=255)
                                    cGTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCt                                                             <  2:2284992/101‑1 (MQ=255)
                                     gTCCCACTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTg                                                            >  2:4365556/1‑101 (MQ=255)
                                           cTTGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATg                                                      <  2:3732863/101‑1 (MQ=255)
                                            ttGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGa                                                     >  2:2703021/1‑101 (MQ=255)
                                             tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAc                                                    >  2:2767196/1‑101 (MQ=255)
                                             tGAGCGGTGAAGTGGGACAGCCCAAAGCGAGGCGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATCAc                                                    >  1:1791806/1‑101 (MQ=255)
                                              gAGCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGAcc                                                   <  1:4616206/101‑1 (MQ=255)
                                                gCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGGCATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaa                                                 <  1:4031665/101‑1 (MQ=255)
                                                gCGGTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCaa                                                 >  2:185800/1‑101 (MQ=255)
                                                  ggTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAg                                               >  1:1359613/1‑101 (MQ=255)
                                                  ggTGAAGTGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAg                                               >  2:1628791/1‑101 (MQ=255)
                                                    tgaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGcc                                             <  2:3645448/96‑1 (MQ=255)
                                                     gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            <  2:2301815/97‑1 (MQ=255)
                                                     gaagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCg                                            >  2:3903139/5‑101 (MQ=255)
                                                      aagtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGa                                           >  1:3099116/4‑101 (MQ=255)
                                                        gtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAg                                         <  1:51315/100‑1 (MQ=255)
                                                        gtGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAAAGTGATTCCCTGCTCATGACCAAAGCCGAAg                                         >  2:1540002/2‑101 (MQ=255)
                                                         tGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGGGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAgg                                        >  1:3118950/1‑101 (MQ=255)
                                                         tGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGACTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAgg                                        <  2:1044527/101‑1 (MQ=255)
                                                             aCAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCa                                    <  2:4287978/101‑1 (MQ=255)
                                                             aCAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCa                                    <  2:1468484/101‑1 (MQ=255)
                                                              cAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCAc                                   <  1:2210599/101‑1 (MQ=255)
                                                              cAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCAc                                   <  1:1411186/101‑1 (MQ=255)
                                                               aGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACg                                  >  1:1005185/1‑101 (MQ=255)
                                                               aGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACg                                  <  1:4092269/101‑1 (MQ=255)
                                                                  ccAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATc                               >  2:4184358/1‑101 (MQ=255)
                                                                   cAAAGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATcc                              <  1:2842579/101‑1 (MQ=255)
                                                                    aaaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCa                             >  1:199708/1‑101 (MQ=255)
                                                                    aaaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCa                             >  1:3964909/1‑101 (MQ=255)
                                                                     aaGCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCAGCTCATGACCAAAGCCGAACGCCCACGATCCAg                            >  1:4010293/1‑101 (MQ=255)
                                                                       gCGAGGTGGGACATGGATGCGGTCGGGCGCNTTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGt                          <  1:77058/101‑1 (MQ=255)
                                                                       gCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGt                          <  2:819549/101‑1 (MQ=255)
                                                                       gCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGt                          <  1:243939/101‑1 (MQ=255)
                                                                       gCGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCAGAAGGCCCACGATCCAGGt                          <  1:2284296/101‑1 (MQ=255)
                                                                        cGAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTg                         >  1:2170430/1‑101 (MQ=255)
                                                                         gAGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGAGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGa                        >  2:4670330/1‑101 (MQ=255)
                                                                          aGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAg                       <  1:1259517/101‑1 (MQ=255)
                                                                          aGGTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAg                       >  1:2782480/1‑101 (MQ=255)
                                                                           ggTGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGc                      >  1:820339/1‑101 (MQ=255)
                                                                             tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    <  1:1427245/101‑1 (MQ=255)
                                                                             tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    >  1:45591/1‑101 (MQ=255)
                                                                             tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    >  2:1486906/1‑101 (MQ=255)
                                                                             tGGGACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCt                    >  1:3283151/1‑101 (MQ=255)
                                                                              gggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCtt                   <  1:3775282/101‑1 (MQ=255)
                                                                              gggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCtt                   <  1:3714752/101‑1 (MQ=255)
                                                                              gggACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCtt                   >  2:2952936/1‑101 (MQ=255)
                                                                                gACATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtca                 >  1:636082/1‑101 (MQ=255)
                                                                                 gcATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcat                <  2:2029310/100‑1 (MQ=255)
                                                                                  cATGGATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTtcatc               <  1:345263/101‑1 (MQ=255)
                                                                                      gATGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGa           <  2:45585/101‑1 (MQ=255)
                                                                                       aTGCGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAc          <  1:2965924/101‑1 (MQ=255)
                                                                                          cGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAcccc       >  1:1858576/1‑101 (MQ=255)
                                                                                          cGGTCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGAcccc       >  1:2700768/1‑101 (MQ=255)
                                                                                             tCGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGaa    >  1:4240902/1‑101 (MQ=255)
                                                                                              cGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAc   >  1:3365697/1‑101 (MQ=255)
                                                                                              cGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAc   <  2:3312687/101‑1 (MQ=255)
                                                                                              cGGGCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAAc   <  2:1303093/101‑1 (MQ=255)
                                                                                               gggCGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACt  >  1:3413918/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGGTGGGACAAGCATTGTTCTGAAAGGAAAATTTGACGTCCCACTTGAGCGGTGAA.TGGGACAGCCCAAAGCGAGGTGGGACATGGATGCGGTCGGGAGCATTTTCCTTGATCGAGTGCCTGCAACGTGATTCCCTGCTCATGACCAAAGCCGAAGGCCCACGATCCAGGTGAGCCTTCATCATGACCCCGAACT  >  NC_014034/2925469‑2925663

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG