Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,713 A→C 3.6% intergenic (+56/‑104) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7130A→C 3.6% 12.2 ‑1.7 ‑0.8221intergenic (+56/‑104)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (7/2):  ref base (104/97):  total (116/105)
Fisher's exact test strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.65e-01

Only 100 of 240 total aligned reads displayed.

CCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAA  >  NC_014034/2950613‑2950813
                                                                                                    |                                                                                                     
cccGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAaca                                                                                                       <  2:2584474/101‑1 (MQ=255)
cccGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCACCGGGATATTTGAGGAAAGATGAAaca                                                                                                       >  2:2263409/1‑101 (MQ=255)
  cGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACg                                                                                                     >  2:3805686/1‑101 (MQ=255)
   ggggTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGc                                                                                                    >  1:2308056/1‑101 (MQ=255)
      gTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGcccc                                                                                                 <  1:1787118/101‑1 (MQ=255)
        gccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCtt                                                                                               >  1:2463908/1‑101 (MQ=255)
         ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGATGCACGATGAAACACGCCCCttt                                                                                              >  2:568558/1‑101 (MQ=255)
         ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCttt                                                                                              <  1:1445082/101‑1 (MQ=255)
         ccgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCttt                                                                                              <  1:2156286/101‑1 (MQ=255)
          cgccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTc                                                                                             >  2:1991941/1‑101 (MQ=255)
          cccccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTc                                                                                             <  1:480125/99‑1 (MQ=255)
           gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                            <  2:3784391/101‑1 (MQ=255)
           gccCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCa                                                                                            >  1:1975510/1‑101 (MQ=255)
            cccTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCAt                                                                                           <  1:108704/101‑1 (MQ=255)
               tCGCCGCAATATGCGGACCTTAATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGCCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                        <  1:1124427/101‑1 (MQ=255)
               tCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                        <  1:4110431/101‑1 (MQ=255)
               tCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCtt                                                                                        <  2:2360023/101‑1 (MQ=255)
                cgccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTg                                                                                       <  1:2385223/101‑1 (MQ=255)
                  ccgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGcc                                                                                     >  1:651125/1‑101 (MQ=255)
                   cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGccc                                                                                    <  1:1732569/101‑1 (MQ=255)
                   cgcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGccc                                                                                    >  1:4197477/1‑101 (MQ=255)
                     cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                  <  2:3236468/101‑1 (MQ=255)
                      aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                 >  2:4413940/1‑101 (MQ=255)
                      aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                 >  2:3323194/1‑101 (MQ=255)
                      aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                 >  1:2838942/1‑101 (MQ=255)
                       atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGACATTTGAGGCAAGATGAAACACCCCCCCTCCATCTTGCCCCcaa                                                                                >  1:3557926/1‑101 (MQ=25)
                        tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGCCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAat                                                                               <  1:2263571/101‑1 (MQ=255)
                        tatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAat                                                                               >  2:3443760/1‑101 (MQ=255)
                           gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                            >  1:1826386/1‑101 (MQ=255)
                           gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                            >  2:2174292/1‑101 (MQ=255)
                            gggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATcc                                                                           <  1:429525/100‑1 (MQ=255)
                             ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGGTATTTGAGGGAAGATGAAAGACGCCCCTTTCATTTTTCCCCAAATATccc                                                                          >  1:852845/1‑101 (MQ=25)
                             ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                          <  2:1169979/101‑1 (MQ=255)
                              gAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.g                                                                        >  2:1418998/1‑101 (MQ=255)
                                aCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggg                                                                      <  1:1260307/101‑1 (MQ=255)
                                    gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                  <  2:1267587/101‑1 (MQ=255)
                                    gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                  <  1:3767988/101‑1 (MQ=255)
                                          tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTg                                                            <  2:2668990/101‑1 (MQ=255)
                                             agcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCg                                                         <  2:640020/101‑1 (MQ=255)
                                               caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                       >  2:2785142/1‑101 (MQ=255)
                                                aagcCGGGGGCTCTGCCCCCGGACCCTCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                      <  1:2803083/101‑1 (MQ=255)
                                                aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTAATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                      >  1:4418369/1‑101 (MQ=255)
                                                aagcCGGAGCCTCCGTCCCCCGCCCCCCGGGATATTTGAGGCAATATGAAACACTCCCCTTTCATATTGCCCCAAATATCCC.GGGGGTCAATTTGCCGTCa                                                      <  2:1490586/101‑1 (MQ=18)
                                                  gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAAGACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                    >  1:4562086/1‑101 (MQ=255)
                                                  gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                    <  2:719133/101‑1 (MQ=255)
                                                    cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                  >  1:741664/1‑101 (MQ=255)
                                                    cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                  <  1:1417590/101‑1 (MQ=255)
                                                     gggggCTCTGCCTCCGGTCCTCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                 <  2:340294/101‑1 (MQ=255)
                                                     gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                 >  2:510913/1‑101 (MQ=255)
                                                      ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaaa                                                <  1:29375/101‑1 (MQ=255)
                                                          ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgg                                            <  2:1941368/101‑1 (MQ=255)
                                                           tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggg                                           <  2:3085969/101‑1 (MQ=255)
                                                            ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGGATTTGCCGTCAGGCCAAGAgggg                                          >  1:3413912/1‑101 (MQ=255)
                                                             ttcccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggggg                                         <  2:825051/99‑1 (MQ=255)
                                                              ttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggc                                       >  1:1373142/9‑99 (MQ=25)
                                                              gcttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGGGAATTGGCC.AAAGGCCAAGAGGGGGCa                                       >  1:4449982/10‑101 (MQ=2)
                                                              gCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGc                                        <  2:3573432/101‑1 (MQ=255)
                                                               tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggca                                      <  2:3005747/94‑4 (MQ=25)
                                                               cttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAg                                      <  1:2544062/93‑1 (MQ=2)
                                                               cccgcGGGCGCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCa                                       <  2:2991917/101‑1 (MQ=255)
                                                                cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                     >  1:3056446/7‑97 (MQ=25)
                                                                ccccGCACCCCCGGGATATGTGAGGCAAGATGAATCTCGCCACTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAg                                      <  2:870315/101‑1 (MQ=25)
                                                                 tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAGcg                                    >  2:4511703/7‑100 (MQ=2)
                                                                    ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcc                                  >  2:4259908/1‑101 (MQ=255)
                                                                    ggACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTcc                                  <  1:3880604/101‑1 (MQ=255)
                                                                     gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                                 <  1:2015559/101‑1 (MQ=255)
                                                                         cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTg                             >  1:3311339/1‑101 (MQ=255)
                                                                           cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcc                           <  2:1003602/101‑1 (MQ=255)
                                                                             ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcccc                         <  2:4141435/101‑1 (MQ=255)
                                                                              gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                        >  1:1740161/1‑101 (MQ=255)
                                                                              gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                        >  1:2109663/1‑101 (MQ=255)
                                                                                tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                      >  1:3142713/1‑101 (MQ=255)
                                                                                tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                      >  2:4289679/1‑101 (MQ=255)
                                                                                tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGc                      >  1:701901/1‑101 (MQ=255)
                                                                                 atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                     <  1:1571555/101‑1 (MQ=255)
                                                                                  tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                    <  2:2608251/101‑1 (MQ=255)
                                                                                  tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                    >  2:4015570/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                 >  1:985354/1‑101 (MQ=255)
                                                                                     gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                 >  2:1555527/1‑101 (MQ=255)
                                                                                      aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                >  2:2904846/1‑101 (MQ=255)
                                                                                      aGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggg                >  2:2194679/1‑101 (MQ=255)
                                                                                       ggCAAGATGAAACTCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga               <  2:3559622/101‑1 (MQ=255)
                                                                                       ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga               <  2:2307894/101‑1 (MQ=255)
                                                                                        gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag              >  2:3725250/1‑101 (MQ=255)
                                                                                         cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg             >  1:3805750/1‑101 (MQ=255)
                                                                                         cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg             >  1:3621717/1‑101 (MQ=255)
                                                                                         cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg             >  1:3001757/1‑101 (MQ=255)
                                                                                          aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc            >  1:269116/1‑101 (MQ=255)
                                                                                          aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc            >  1:372155/1‑101 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        <  1:369008/101‑1 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        <  1:4543567/101‑1 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        >  2:1043623/1‑101 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        <  2:250762/101‑1 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        <  2:2432419/101‑1 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        >  2:1689929/1‑101 (MQ=255)
                                                                                              tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa        >  2:2033473/1‑101 (MQ=255)
                                                                                               gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc       >  2:3159482/1‑101 (MQ=255)
                                                                                                aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt      <  2:1737940/101‑1 (MQ=255)
                                                                                                  acacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTgg    >  1:1619829/1‑101 (MQ=255)
                                                                                                    acGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaa  >  1:2592803/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CCCGGGGTGCCGCCCTCGCCGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAA  >  NC_014034/2950613‑2950813

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG