Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,950,732 C→G 3.1% intergenic (+75/‑85) RCAP_rcc02761 → / → RCAP_rcc02762 glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,950,7320C→G 3.1% 7.6 ‑2.0 ‑0.1222intergenic (+75/‑85)RCAP_rcc02761/RCAP_rcc02762glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (119/96):  total (125/97)
Fisher's exact test strand distribution p-value = 1.40e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.50e-01

Only 100 of 237 total aligned reads displayed.

CGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTG  >  NC_014034/2950632‑2950831
                                                                                                    |                                                                                                    
 gcAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGcccc                                                                                                     >  1:4141723/1‑101 (MQ=255)
  cAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCa                                                                                                    <  2:3236468/101‑1 (MQ=255)
   aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                                   >  2:4413940/1‑101 (MQ=255)
   aaTATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaa                                                                                                   >  2:3323194/1‑101 (MQ=255)
    atatGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCaaa                                                                                                  >  2:2217433/1‑101 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                >  1:2328251/1‑101 (MQ=255)
      atGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAata                                                                                                <  1:1606877/101‑1 (MQ=255)
       tGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAatat                                                                                               <  2:3516129/101‑1 (MQ=255)
        gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                                              >  1:1826386/1‑101 (MQ=255)
        gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                                              >  2:4015178/1‑101 (MQ=255)
        gCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATc                                                                                              <  2:3785089/101‑1 (MQ=255)
         gggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATcc                                                                                             <  1:429525/100‑1 (MQ=255)
          ggAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATccc                                                                                            <  1:3805971/101‑1 (MQ=255)
            aaCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.gg                                                                                         <  2:4205115/101‑1 (MQ=255)
             aCTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.ggg                                                                                        <  1:1260307/101‑1 (MQ=255)
                 gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                                    <  1:1504418/101‑1 (MQ=255)
                 gATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTg                                                                                    <  1:3767988/101‑1 (MQ=255)
                    ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                                                 >  2:609496/1‑101 (MQ=255)
                    ttCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAAt                                                                                 <  1:1710253/101‑1 (MQ=255)
                     tCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAAtt                                                                                >  2:1872361/1‑101 (MQ=255)
                       tGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTg                                                                              <  1:3345757/101‑1 (MQ=255)
                         aagcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGcc                                                                            >  1:2608436/1‑101 (MQ=255)
                           gcaagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGt                                                                          <  2:217355/101‑1 (MQ=255)
                            caagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTc                                                                         <  1:1992076/101‑1 (MQ=255)
                             aagcCGGGGGCTCTGCCCCCGGACCCTCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                        <  1:2803083/101‑1 (MQ=255)
                             aagcCGGAGCCTCCGTCCCCCGCCCCCCGGGATATTTGAGGCAATATGAAACACTCCCCTTTCATATTGCCCCAAATATCCC.GGGGGTCAATTTGCCGTCa                                                                        <  2:1490586/101‑1 (MQ=18)
                               gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                      <  2:719133/101‑1 (MQ=255)
                               gcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                      >  1:1341464/1‑101 (MQ=255)
                                 cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGGTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                    >  1:3534555/1‑101 (MQ=255)
                                 cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                    <  1:1417590/101‑1 (MQ=255)
                                 cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                    <  2:2448282/101‑1 (MQ=255)
                                 cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                    >  1:741664/1‑101 (MQ=255)
                                  gggggCTCTGCCTCCGGTCCTCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                                   <  2:340294/101‑1 (MQ=255)
                                  gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                                   >  2:510913/1‑101 (MQ=255)
                                   ggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaaa                                                                  >  1:4467960/1‑101 (MQ=255)
                                    gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAag                                                                 >  1:2046595/1‑101 (MQ=255)
                                       ctctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgg                                                              >  2:2185908/1‑101 (MQ=255)
                                        tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggg                                                             >  2:2679140/1‑101 (MQ=255)
                                        tctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggg                                                             <  2:3085969/101‑1 (MQ=255)
                                         ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAgggg                                                            >  2:4284328/1‑101 (MQ=255)
                                          ttcccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAggggg                                                           <  2:825051/99‑1 (MQ=255)
                                           gcttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGGGAATTGGCC.AAAGGCCAAGAGGGGGCa                                                         >  1:4449982/10‑101 (MQ=2)
                                            tcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggca                                                        <  2:3005747/94‑4 (MQ=25)
                                            cttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAg                                                        <  1:280985/93‑1 (MQ=2)
                                            cttcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAAGAGGGGGCAg                                                        <  1:2544062/93‑1 (MQ=2)
                                             cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                                       >  1:3056446/7‑97 (MQ=25)
                                             cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAAGAggggggcag                                                       >  2:2659336/7‑97 (MQ=25)
                                             ccccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAg                                                        >  1:2469077/1‑101 (MQ=255)
                                             ccccGCACCCCCGGGATATGTGAGGCAAGATGAATCTCGCCACTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAg                                                        <  2:870315/101‑1 (MQ=25)
                                                cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTc                                                     >  1:3588810/1‑101 (MQ=255)
                                                  gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                                                   <  1:3733935/101‑1 (MQ=255)
                                                  gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccc                                                   <  1:707198/101‑1 (MQ=255)
                                                    cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTccccc                                                 <  2:1092999/101‑1 (MQ=255)
                                                     ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCt                                                <  1:2217595/101‑1 (MQ=255)
                                                        cGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcc                                             <  2:1003602/101‑1 (MQ=255)
                                                         gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGccc                                            >  1:4243804/1‑101 (MQ=255)
                                                          ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGcccc                                           <  2:4141435/101‑1 (MQ=255)
                                                           gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                                          >  1:1740161/1‑101 (MQ=255)
                                                           gATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCg                                          >  2:1379935/1‑101 (MQ=255)
                                                              atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCt                                       >  2:2424968/1‑101 (MQ=255)
                                                               tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTg                                      >  1:4671355/1‑101 (MQ=255)
                                                                  gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                   >  2:1555527/1‑101 (MQ=255)
                                                                  gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                   >  2:442864/1‑101 (MQ=255)
                                                                  gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                   >  1:985354/1‑101 (MQ=255)
                                                                    ggCAAGATGAAACTCGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                 <  2:3559622/101‑1 (MQ=255)
                                                                     gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                >  2:3725250/1‑101 (MQ=255)
                                                                      cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg                               >  1:3001757/1‑101 (MQ=255)
                                                                      cAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTgagggagg                               >  1:2114588/1‑101 (MQ=255)
                                                                       aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                              >  1:269116/1‑101 (MQ=255)
                                                                           tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                          >  2:1689929/1‑101 (MQ=255)
                                                                           tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                          >  2:2033473/1‑101 (MQ=255)
                                                                           tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                          <  2:250762/101‑1 (MQ=255)
                                                                           tGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                          <  1:369008/101‑1 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCCGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                        >  1:4023105/1‑101 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                        <  1:3225505/101‑1 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                        <  1:4545126/101‑1 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                        <  2:1737940/101‑1 (MQ=255)
                                                                             aaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACt                        <  2:1975375/101‑1 (MQ=255)
                                                                                cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                     >  1:2883591/1‑101 (MQ=255)
                                                                                cacGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGa                     >  2:230293/1‑101 (MQ=255)
                                                                                  cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                   >  1:519278/1‑101 (MQ=255)
                                                                                   gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                  <  2:2943476/101‑1 (MQ=255)
                                                                                   gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                  >  2:1182366/1‑101 (MQ=255)
                                                                                    ccccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAcc                 <  2:4530302/101‑1 (MQ=255)
                                                                                     cccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCt                <  2:3198555/101‑1 (MQ=255)
                                                                                       cttccttCTTGCCCAAAATACCCG.GGGGGTGATTTTCCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg              <  2:701826/95‑1 (MQ=37)
                                                                                       cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg              <  2:3413658/101‑1 (MQ=255)
                                                                                       cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg              <  2:1110329/101‑1 (MQ=255)
                                                                                       cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg              <  1:4381526/101‑1 (MQ=255)
                                                                                       cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg              >  1:1776111/1‑101 (MQ=255)
                                                                                        tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa             <  2:4564370/101‑1 (MQ=255)
                                                                                        tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa             >  1:898753/1‑101 (MQ=255)
                                                                                         ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa            >  2:2132818/1‑101 (MQ=255)
                                                                                          tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg           <  1:609557/101‑1 (MQ=255)
                                                                                           cATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa          >  1:1197929/1‑101 (MQ=255)
                                                                                           cATCTTGCCCCAAATATCCC.GGGGGTGAATTGGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa          <  2:3142469/101‑1 (MQ=255)
                                                                                            aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc         >  2:2903492/1‑101 (MQ=255)
                                                                                            aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc         <  2:2838726/101‑1 (MQ=255)
                                                                                            aTCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAc         >  2:2582892/1‑101 (MQ=255)
                                                                                                tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc     >  1:3647673/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CGCAATATGCGGAACTTGATTTCTGAAGCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAAGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTG  >  NC_014034/2950632‑2950831

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG