Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,953,304 T→C 2.9% intergenic (+193/+185) RCAP_rcc02764 → / ← ilvE1 hypothetical protein/branched‑chain‑amino‑acid transaminase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,953,3040T→C 2.9% 4.4 ‑0.9 ‑0.8195intergenic (+193/+185)RCAP_rcc02764/ilvE1hypothetical protein/branched‑chain‑amino‑acid transaminase
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (114/75):  total (119/76)
Fisher's exact test strand distribution p-value = 4.07e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.52e-01

Only 100 of 217 total aligned reads displayed.

CGTGCGCAGACGGCCCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGG  >  NC_014034/2953204‑2953404
                                                                                                      |                                                                                                       
cGTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTc                                                                                                        <  1:1014192/101‑1 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTca                                                                                                       >  1:1101473/1‑101 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTca                                                                                                       >  1:2986206/1‑101 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTca                                                                                                       >  1:2539084/1‑101 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTca                                                                                                       >  1:1840745/1‑101 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTca                                                                                                       >  1:1714746/1‑101 (MQ=255)
   gagcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTcacc                                                                                                     >  2:221566/3‑101 (MQ=255)
     gcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTcaccac                                                                                                   <  1:3616355/101‑1 (MQ=255)
      cAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCaccacc                                                                                                  >  1:3059153/1‑101 (MQ=255)
      cAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCaccacc                                                                                                  >  2:1034069/1‑101 (MQ=255)
       aGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCAccg                                                                                                 <  1:1530160/101‑1 (MQ=255)
        gACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCAccgc                                                                                                <  2:359260/101‑1 (MQ=255)
           cggcccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCAGCAGTCACCAccgccg                                                                                              >  2:1529075/4‑101 (MQ=255)
            ggcccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCgg                                                                                             >  1:3344179/3‑101 (MQ=255)
              cccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCgg.gg                                                                                          >  2:275881/1‑101 (MQ=255)
                  ccTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCa                                                                                      >  1:2287984/1‑101 (MQ=255)
                   cTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCaa                                                                                     <  1:4391688/101‑1 (MQ=255)
                    ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCaaa                                                                                    <  2:2484314/101‑1 (MQ=255)
                    ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCaaa                                                                                    <  1:3200353/101‑1 (MQ=255)
                      ggTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.Gt                                                                                 >  1:3290109/1‑101 (MQ=255)
                       gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTc                                                                                <  1:2511019/101‑1 (MQ=255)
                       gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTc                                                                                <  2:4369232/101‑1 (MQ=255)
                       gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTc                                                                                >  1:876165/1‑101 (MQ=255)
                       gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGACGGTGACGCGGCCAT..GCGAGACCTCTGCAGTCACTACCGCCGG.GGActctagtc                                                                                 >  2:2325145/1‑94 (MQ=38)
                          ttttCGGTGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGCGACGCGGCGAT..GCGAGACGTCTGCAGTCACCACCGNCGG.GGACCAAC.Gtaggc                                                                             >  2:1411821/1‑97 (MQ=255)
                           tttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCg                                                                            >  2:232488/1‑101 (MQ=255)
                            ttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCgg                                                                           >  1:3753889/1‑101 (MQ=255)
                            ttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCgg                                                                           <  1:4350591/101‑1 (MQ=255)
                            ttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCgg                                                                           >  1:817459/1‑101 (MQ=255)
                             tCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGc                                                                          >  2:3697/1‑101 (MQ=255)
                                 gggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAgc                                                                      <  2:2538910/101‑1 (MQ=255)
                                 gggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAgc                                                                      >  1:1490887/1‑101 (MQ=255)
                                    cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGcgcc                                                                   <  1:1166844/101‑1 (MQ=255)
                                    cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGcgcc                                                                   <  1:2908150/101‑1 (MQ=255)
                                     gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGcgccc                                                                  >  1:2791830/1‑101 (MQ=255)
                                     gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGcgccc                                                                  >  2:1354830/1‑101 (MQ=255)
                                        ttCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGcgcccgcc                                                               >  2:3841204/1‑101 (MQ=255)
                                         tctcTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCgcccgccc                                                              <  2:876089/101‑1 (MQ=255)
                                          ctctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGcccgcccc                                                             <  1:3700/101‑1 (MQ=255)
                                          ctctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGcccgcccc                                                             <  2:2729217/101‑1 (MQ=255)
                                          ctctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGcccgcccc                                                             >  2:3625935/1‑101 (MQ=255)
                                           tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGcccgccccg                                                            >  1:1445784/1‑101 (MQ=255)
                                            ctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGcccgccccgc                                                           >  1:1978269/1‑101 (MQ=255)
                                               gaaGGGCCTTACCTGGGCGCCGCCGGTGGCGCGGGCAT..GGGAGACATCCGCAGGCCCCCCCGCCGG.GGACCAAA.GGCCCCGGCCAGCGCCCGCCCCGCCCa                                                        <  2:2260111/100‑1 (MQ=16)
                                               aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCa                                                        >  1:70446/1‑101 (MQ=255)
                                               aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCa                                                        <  1:1696357/101‑1 (MQ=255)
                                                aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCCCCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCAt                                                       <  2:4606119/101‑1 (MQ=255)
                                                aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCCCCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCAt                                                       <  1:4295503/101‑1 (MQ=255)
                                                aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCAt                                                       <  1:3405820/101‑1 (MQ=255)
                                                aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCAt                                                       <  1:3962022/101‑1 (MQ=255)
                                                  gcgcCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAg                                                     <  1:4229512/101‑1 (MQ=255)
                                                    gcCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                   >  1:582816/1‑101 (MQ=255)
                                                    gcCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                   <  2:2592383/101‑1 (MQ=255)
                                                     ccTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCgg                                                  <  1:1284948/101‑1 (MQ=255)
                                                      cTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCggg                                                 >  1:2763340/1‑101 (MQ=255)
                                                       tGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGgc                                                >  1:4194799/1‑101 (MQ=255)
                                                         aCTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGgcgc                                              <  1:2217851/101‑1 (MQ=255)
                                                            tGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                           <  1:2143271/101‑1 (MQ=255)
                                                            tGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                           >  2:113902/1‑101 (MQ=255)
                                                             ggCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCCGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                          <  1:4249479/101‑1 (MQ=255)
                                                              gCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct.c                                        <  1:2393281/101‑1 (MQ=255)
                                                               ccGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.cg                                       <  2:667977/101‑1 (MQ=255)
                                                               ccGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.cg                                       >  2:1107400/1‑101 (MQ=255)
                                                               ccGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.cg                                       <  2:813175/101‑1 (MQ=255)
                                                                cgcgGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCACCGCCCATAGCGGGCGCTTCT.cgc                                      >  2:54788/1‑101 (MQ=255)
                                                                   ggCCGGTGACGCGGCCAT..GCGAAACAGCGGCAGCAACAGCCGCCGGTGCCCCAAA.GGCACCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCCTCC.ccccc                                    >  1:2437045/1‑101 (MQ=25)
                                                                       ggTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgg                               >  2:1438939/1‑101 (MQ=255)
                                                                       ggTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgg                               >  2:3863420/1‑101 (MQ=255)
                                                                        gTGACGCGGCCCTGCGCGGGACATCTGCAGCAAGACCCGCCGG.GGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGGCTCT.cccccccc                                 >  1:3908197/1‑101 (MQ=25)
                                                                        gTGACGCGGCCAT..GCGAGACATCTGCAGTCCCCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCggg                              <  2:4078603/101‑1 (MQ=255)
                                                                         tGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgggg                             >  1:3257949/1‑101 (MQ=255)
                                                                         tGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgggg                             >  1:463059/1‑101 (MQ=255)
                                                                         tGACGCGGCCAT..GCGAAACAGCGGCAGCAACAGCCGCCGGTGCCCCAAA.GGCACCGGCCAGCGCCCGCCCCGCCCCTGGCGGGGGGCTCTCCGCCCCCCgg                               >  2:824299/1‑101 (MQ=25)
                                                                         ggACGCGGCCAT..GCGAGACATCTGCAGTCCCCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgggg                             <  1:2071326/100‑1 (MQ=255)
                                                                          gccgcgGCCAG..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGc                            <  2:3753614/99‑1 (MQ=255)
                                                                          gACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGc                            <  1:1684268/101‑1 (MQ=255)
                                                                           tcgcgCCCTT..GCGAGACACCTGCAGTCACCCCCGCCGG.GGACCAAA.GCCCCCGGCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCa                           <  2:2070708/100‑1 (MQ=18)
                                                                           ccgcgGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCa                           <  1:3215903/100‑1 (MQ=255)
                                                                               ggCCAT..GCGAGACATCTGCAGTCCCCCCCGCCGG.GGACCAAA.GCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGgcg                       <  1:2411942/101‑1 (MQ=255)
                                                                               cgCCAT..GCGCGGCATCTGCGTCCCCCGCCGCCGG.GGCCGGAA.CTCCCCGTCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGgcg                       <  1:631489/100‑1 (MQ=12)
                                                                                 ccAT..GCGAAACAGCGGCAGCAACAGCCGCCGGTGCCCCAAA.GGCACCGGCCAGCGCCCGCCCCGCCCATGGCGGGCGCTTCT.CGCCCCCCCGGGGCGgcgc                      >  1:779504/1‑101 (MQ=21)
                                                                                  cAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCNGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCg                    >  1:613337/1‑101 (MQ=255)
                                                                                  cAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCg                    <  2:1840619/101‑1 (MQ=255)
                                                                                   aT..GCGAGACATCTGCAGCCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCgg                   <  2:3869862/101‑1 (MQ=255)
                                                                                       gCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGcc                 >  1:676430/1‑101 (MQ=255)
                                                                                         gagaCATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCctc               >  1:2606207/1‑101 (MQ=255)
                                                                                          agccATCTGCGGCCACCACCGCGGG.GCACCAAA.GCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCctct              <  2:3289864/98‑1 (MQ=17)
                                                                                           gaCATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTg             >  2:2657096/1‑101 (MQ=255)
                                                                                             cATCTGCAGTCCCCACCGCGGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGtt           <  1:2744729/101‑1 (MQ=255)
                                                                                             cATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCGTAGCGGGCGCTTCT.CGCCCGCCCGGGCAGGCGCCGGCCTCTGtt           >  2:2178822/1‑101 (MQ=255)
                                                                                             cATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGtt           >  1:357093/1‑101 (MQ=255)
                                                                                               tCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgc         >  2:4413570/1‑101 (MQ=255)
                                                                                                 tGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgc       >  2:3370012/1‑101 (MQ=255)
                                                                                                  gCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcg      >  2:1224451/1‑101 (MQ=255)
                                                                                                  gCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcc      >  2:3123860/1‑100 (MQ=255)
                                                                                                   cAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGGTgcgcgc     >  2:4293623/1‑101 (MQ=255)
                                                                                                    cgTCCCCCCCGCCGG.GTACCAAA.GCCCCCGNCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg    <  1:54794/100‑1 (MQ=17)
                                                                                                    aGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg    >  2:2973395/1‑101 (MQ=255)
                                                                                                    aGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCCCCCATAGCGGGGGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCCCTGTTgcgcggg    >  1:1235042/1‑99 (MQ=255)
                                                                                                      tCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCggg  >  1:1366013/1‑101 (MQ=255)
                                                                                                      |                                                                                                       
CGTGCGCAGACGGCCCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCAT..GCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAA.GTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGG  >  NC_014034/2953204‑2953404

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG