Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,953,305 C→A 2.8% intergenic (+194/+184) RCAP_rcc02764 → / ← ilvE1 hypothetical protein/branched‑chain‑amino‑acid transaminase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,953,3050C→A 2.8% 5.2 ‑1.8 ‑1.4176intergenic (+194/+184)RCAP_rcc02764/ilvE1hypothetical protein/branched‑chain‑amino‑acid transaminase
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (102/69):  total (107/69)
Fisher's exact test strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.17e-02

Only 100 of 219 total aligned reads displayed.

CGTGCGCAGACGGCCCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGG  >  NC_014034/2953204‑2953405
                                                                                                     |                                                                                                      
cGTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTc                                                                                                        <  1:1014192/101‑1 (MQ=255)
cGTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGAATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTc                                                                                                        <  1:3935909/101‑1 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTca                                                                                                       >  1:1101473/1‑101 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTca                                                                                                       >  1:1840745/1‑101 (MQ=255)
 gTGAGCAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGCGACGCGGCCATGCGAGACATCTGCAGTca                                                                                                       >  1:4452763/1‑101 (MQ=255)
  tgagcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcac                                                                                                      >  2:1508821/4‑101 (MQ=255)
  tgagcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcac                                                                                                      >  2:3427416/4‑101 (MQ=255)
  ggagcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcac                                                                                                      <  1:619949/98‑1 (MQ=255)
   gagcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcacc                                                                                                     >  1:3285671/3‑101 (MQ=255)
     gcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcaccac                                                                                                   <  1:3671865/101‑1 (MQ=255)
       aGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAccg                                                                                                 <  1:1530160/101‑1 (MQ=255)
        gACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAccgc                                                                                                <  2:359260/101‑1 (MQ=255)
        gACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAccgc                                                                                                <  1:490960/101‑1 (MQ=255)
          acggcccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAccgcc                                                                                               <  1:565135/97‑1 (MQ=255)
            ggcccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCgg                                                                                             >  2:2510845/3‑101 (MQ=255)
             gcccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCgg.g                                                                                           >  2:376780/2‑101 (MQ=255)
             gcccGCCTTGGTCTTTTCGGGGCCGCATTCTCTGAAAGCGCCTGACTTAACCGCGGCCGGTGACGCGGCCATGCGAGACATATGCAGTCACCACAGCCgg.g                                                                                           >  1:4355451/2‑101 (MQ=38)
               ccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGa                                                                                         <  2:4375342/101‑1 (MQ=255)
               ccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGAAATCTGCGGTCACCACCGCCGG.GGa                                                                                         >  1:3872323/1‑101 (MQ=255)
                cGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGAc                                                                                        >  1:4230499/1‑101 (MQ=255)
                    ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCaaa                                                                                    <  1:3200353/101‑1 (MQ=255)
                    ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAACGCCGG.GGACCaaa                                                                                    <  1:927482/101‑1 (MQ=255)
                       gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTc                                                                                 >  1:801599/1‑101 (MQ=255)
                       gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTc                                                                                 <  1:2511019/101‑1 (MQ=255)
                           tttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCTGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCg                                                                             <  1:3992663/101‑1 (MQ=255)
                                ggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAg                                                                        >  2:4607027/1‑101 (MQ=255)
                                 gggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAgc                                                                       >  1:1490887/1‑101 (MQ=255)
                                    cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgcc                                                                    <  1:1166844/101‑1 (MQ=255)
                                    cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgcc                                                                    >  1:664621/1‑101 (MQ=255)
                                     gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgccc                                                                   >  1:2951347/1‑101 (MQ=255)
                                     gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgccc                                                                   >  1:2791830/1‑101 (MQ=255)
                                     gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgccc                                                                   <  2:1352582/101‑1 (MQ=255)
                                     gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgccc                                                                   <  2:2325000/101‑1 (MQ=255)
                                        ttCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGcgcccgcc                                                                >  2:4146500/1‑101 (MQ=255)
                                         tctcTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCgcccgccc                                                               <  2:876089/101‑1 (MQ=255)
                                          ctctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGcccgcccc                                                              >  2:3625935/1‑101 (MQ=255)
                                           tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGcccgccccg                                                             <  1:2250988/101‑1 (MQ=255)
                                           tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGcccgccccg                                                             >  1:1445784/1‑101 (MQ=255)
                                           tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGcccgccccg                                                             >  2:3845895/1‑101 (MQ=255)
                                           tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGcccgccccg                                                             >  1:2592568/1‑101 (MQ=255)
                                            ctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGcccgccccgc                                                            >  1:1978269/1‑101 (MQ=255)
                                              gAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCcgccccgccc                                                          >  2:2413567/1‑101 (MQ=255)
                                               gaaGGGCCTTACCTGGGCGCCGCCGGTGGCGCGGGCATGGGAGACATCCGCAGGCCCCCCCGCCGG.GGACCAAAGGCCCCGGCCAGCGCCCGCCCCGCCCa                                                         <  2:2260111/100‑1 (MQ=16)
                                               aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCa                                                         <  1:1696357/101‑1 (MQ=255)
                                               aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCa                                                         >  1:704463/1‑101 (MQ=255)
                                               aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCa                                                         >  2:2705879/1‑101 (MQ=255)
                                                aaGCGCCTGACTTGGCCGCAGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCac                                                        <  1:3405819/101‑2 (MQ=255)
                                                    gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                    <  2:1104142/101‑1 (MQ=255)
                                                    gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                    >  2:3864672/1‑101 (MQ=255)
                                                    gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                    <  2:2592383/101‑1 (MQ=255)
                                                     ccTGGCTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCgg                                                   <  2:1537764/101‑1 (MQ=255)
                                                     ccTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCgg                                                   <  1:1284948/101‑1 (MQ=255)
                                                      cTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCggg                                                  >  2:2071186/1‑101 (MQ=255)
                                                      cTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCggg                                                  >  1:2763340/1‑101 (MQ=255)
                                                       tGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGgc                                                 >  1:4194799/1‑101 (MQ=255)
                                                       tGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGgc                                                 >  2:1167951/1‑101 (MQ=255)
                                                           ttGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCtt                                             >  1:843136/1‑101 (MQ=255)
                                                            tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                            >  2:2757302/1‑101 (MQ=255)
                                                            tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                            <  1:2143271/101‑1 (MQ=255)
                                                             ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                           <  2:2211149/101‑1 (MQ=255)
                                                             ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                           >  2:2324470/1‑101 (MQ=255)
                                                             ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                           <  2:1112496/101‑1 (MQ=255)
                                                             ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                           >  1:331194/1‑101 (MQ=255)
                                                              gCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCCCCCCCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct.c                                         <  2:3318188/101‑1 (MQ=255)
                                                              gCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct.c                                         <  1:2393281/101‑1 (MQ=255)
                                                               ccGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.cg                                        >  1:2297993/1‑101 (MQ=255)
                                                               ccGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.cg                                        <  2:813175/101‑1 (MQ=255)
                                                               ccGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.cg                                        <  2:667977/101‑1 (MQ=255)
                                                                   ggCCGGTGACGCGGCCATGCGAAACAGCGGCAGCAACAGCCGCCGGTGCCCCAAAGGCACCGGCCAGCGCCCGCCCCGCCCATGGCGGGGGCCTCC.ccccc                                     >  1:2437045/1‑101 (MQ=25)
                                                                      cGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCccgccg                                 <  2:3130186/101‑1 (MQ=255)
                                                                       ggTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgg                                >  1:702560/1‑101 (MQ=255)
                                                                         tGACGCGGCCATGCGAAACAGCGGCAGCAACAGCCGCCGGTGCCCCAAAGGCACCGGCCAGCGCCCGCCCCGCCCCTGGCGGGGGGCTCTCCGCCCCCCgg                                >  2:824299/1‑101 (MQ=25)
                                                                         ggACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCgggg                              <  1:2071326/100‑1 (MQ=255)
                                                                          gccgcgGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGc                             <  2:1394486/99‑1 (MQ=255)
                                                                          gACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGc                             >  2:4368090/1‑101 (MQ=255)
                                                                          gACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGc                             <  1:1684268/101‑1 (MQ=255)
                                                                          gACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCCCCCCGCCCATAGCGGGCGCTGCT.CGCCCGCCGGGGc                             >  1:4297776/1‑101 (MQ=255)
                                                                           ccgcgGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCa                            <  1:3215903/100‑1 (MQ=255)
                                                                              cgGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGgc                         >  1:4290684/1‑101 (MQ=255)
                                                                              cgGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGgc                         >  1:587500/1‑101 (MQ=255)
                                                                               ggCCATGCGAGACATCTGCAGTCCCCCCCGCCGG.GGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGgcg                        <  1:2411942/101‑1 (MQ=255)
                                                                                gCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGgcgc                       <  1:2662422/101‑1 (MQ=255)
                                                                                   aTGCGAGACATCTGCAGCCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCgg                    <  2:3869862/101‑1 (MQ=255)
                                                                                   aTGAGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCgg                    >  2:1429447/1‑101 (MQ=255)
                                                                                    tGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGc                   >  2:4523360/1‑101 (MQ=255)
                                                                                     gCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGcc                  >  1:1723270/1‑101 (MQ=255)
                                                                                     gCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGcc                  >  2:3736651/1‑101 (MQ=255)
                                                                                        agaCATCTGCAGCCACCACCGCCGG.GGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCctct               <  2:2951118/101‑1 (MQ=255)
                                                                                         gaCATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTg              >  2:2657096/1‑101 (MQ=255)
                                                                                           cATCTGCAGTCCCCACCGCGGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGtt            <  1:2744729/101‑1 (MQ=255)
                                                                                           cATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGtt            >  1:357093/1‑101 (MQ=255)
                                                                                             tCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgc          >  1:3746842/1‑101 (MQ=255)
                                                                                              cTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg         >  1:3136007/1‑101 (MQ=255)
                                                                                              cTGCAGCCCCCACCGCCGG.GGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg         <  1:3006180/101‑1 (MQ=255)
                                                                                                gcatccaccccCGCCGG.GCACCAAATCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGTCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcg       <  2:613276/96‑1 (MQ=17)
                                                                                                  aGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg     >  2:1916288/1‑101 (MQ=255)
                                                                                                  aGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGGTGcccgcg     >  1:3450732/1‑101 (MQ=255)
                                                                                                  aGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCCCCCATAGCGGGGGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCCCTGTTgcgcggg     >  1:1235042/1‑99 (MQ=255)
                                                                                                    tCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCggg   >  1:1366013/1‑101 (MQ=255)
                                                                                                     caccacCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGGCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCgggg  >  2:3105181/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CGTGCGCAGACGGCCCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGG.GGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCT.CGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGG  >  NC_014034/2953204‑2953405

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG