Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,953,308 C→A 2.9% intergenic (+197/+181) RCAP_rcc02764 → / ← ilvE1 hypothetical protein/branched‑chain‑amino‑acid transaminase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,953,3080C→A 2.9% 4.3 ‑1.8 ‑0.4174intergenic (+197/+181)RCAP_rcc02764/ilvE1hypothetical protein/branched‑chain‑amino‑acid transaminase
Reads supporting (aligned to +/- strand):  new base (5/0):  ref base (100/69):  total (105/69)
Fisher's exact test strand distribution p-value = 1.58e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.66e-01

Only 100 of 208 total aligned reads displayed.

GCGCAGACGGCCCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCA  >  NC_014034/2953207‑2953407
                                                                                                     |                                                                                                   
gagcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcacc                                                                                                     >  2:221566/3‑101 (MQ=255)
  gcAGACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTcaccac                                                                                                   <  1:3671865/101‑1 (MQ=255)
     gACGG.CCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAccgc                                                                                                <  2:359260/101‑1 (MQ=255)
        cggcccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCAGCAGTCACCAccgccg                                                                                              >  2:1529075/4‑101 (MQ=255)
          gcccGCCTTGGTCTTTTCGGGGCCGCATTCTCTGAAAGCGCCTGACTTAACCGCGGCCGGTGACGCGGCCATGCGAGACATATGCAGTCACCACAGCCggg                                                                                            >  1:4355451/2‑101 (MQ=38)
            ccGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGAAATCTGCGGTCACCACCGCCGGGGa                                                                                          >  1:3872323/1‑101 (MQ=255)
             cGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGAc                                                                                         >  1:4230499/1‑101 (MQ=255)
              gCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGAcc                                                                                        <  1:1627490/101‑1 (MQ=255)
                 ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGCCCaaa                                                                                     >  2:1442971/1‑101 (MQ=255)
                 ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCaaa                                                                                     <  1:3200353/101‑1 (MQ=255)
                 ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCaaa                                                                                     >  1:278019/1‑101 (MQ=255)
                 ttGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCAACGCCGGGGACCaaa                                                                                     <  1:927482/101‑1 (MQ=255)
                  tGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAg                                                                                    <  1:1693358/101‑1 (MQ=255)
                   ggTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGt                                                                                   >  1:668039/1‑101 (MQ=255)
                   ggTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGt                                                                                   >  1:3290109/1‑101 (MQ=255)
                    gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTc                                                                                  >  1:801599/1‑101 (MQ=255)
                    gTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTc                                                                                  <  2:4369232/101‑1 (MQ=255)
                       ttttCGGTGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGCGACGCGGCGATGCGAGACGTCTGCAGTCACCACCGNCGGGGACCAACGtaggc                                                                               >  2:1411821/1‑97 (MQ=255)
                        tttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCg                                                                              <  1:2416757/101‑1 (MQ=255)
                         ttCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCgg                                                                             >  1:3753889/1‑101 (MQ=255)
                          tCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGc                                                                            >  2:3697/1‑101 (MQ=255)
                           cGGGGCCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCACCGGcc                                                                           >  2:631428/1‑101 (MQ=255)
                            gggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCa                                                                          <  1:3599472/101‑1 (MQ=255)
                            gggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCa                                                                          <  1:1407608/101‑1 (MQ=255)
                             ggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAg                                                                         <  1:4217902/101‑1 (MQ=255)
                             ggggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAg                                                                         <  1:1488316/101‑1 (MQ=255)
                              gggCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAgc                                                                        <  2:2538910/101‑1 (MQ=255)
                                gcgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAgcgc                                                                      <  1:1034152/101‑1 (MQ=255)
                                 cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGcgcc                                                                     <  1:2908150/101‑1 (MQ=255)
                                 cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGcgcc                                                                     <  1:1166844/101‑1 (MQ=255)
                                 cgcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGcgcc                                                                     >  2:815262/1‑101 (MQ=255)
                                  gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGcgccc                                                                    <  2:2325000/101‑1 (MQ=255)
                                  gcATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGcgccc                                                                    >  2:1354830/1‑101 (MQ=255)
                                       ctctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGcccgcccc                                                               >  2:3625935/1‑101 (MQ=255)
                                        tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGcccgccccg                                                              >  2:3845895/1‑101 (MQ=255)
                                        tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGcccgccccg                                                              <  1:2250988/101‑1 (MQ=255)
                                        tctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGcccgccccg                                                              <  1:2409508/101‑1 (MQ=255)
                                         ctGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGcccgccccgc                                                             >  1:1978269/1‑101 (MQ=255)
                                           gAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCcgccccgccc                                                           >  2:2413567/1‑101 (MQ=255)
                                            gaaGGGCCTTACCTGGGCGCCGCCGGTGGCGCGGGCATGGGAGACATCCGCAGGCCCCCCCGCCGGGGACCAAAGGCCCCGGCCAGCGCCCGCCCCGCCCa                                                          <  2:2260111/100‑1 (MQ=16)
                                            aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCa                                                          >  1:704463/1‑101 (MQ=255)
                                            aaaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCa                                                          >  2:2705879/1‑101 (MQ=255)
                                             aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCAt                                                         <  2:4606119/101‑1 (MQ=255)
                                             aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCAt                                                         <  1:3962022/101‑1 (MQ=255)
                                             aaGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCAAt                                                         <  1:2663052/101‑1 (MQ=255)
                                             aaGCGCCTGACTTGGCCGCAGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCac                                                         <  1:3405819/101‑2 (MQ=255)
                                                 gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                     >  1:2502885/1‑101 (MQ=255)
                                                 gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                     >  2:3864672/1‑101 (MQ=255)
                                                 gcCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCg                                                     >  1:582816/1‑101 (MQ=255)
                                                  ccTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCgg                                                    <  1:1284948/101‑1 (MQ=255)
                                                    tGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGgc                                                  <  2:782135/101‑1 (MQ=255)
                                                      aCTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGgcgc                                                >  1:2104318/1‑101 (MQ=255)
                                                        ttGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCtt                                              >  1:843136/1‑101 (MQ=255)
                                                         tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                             >  2:2757302/1‑101 (MQ=255)
                                                         tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                             >  2:113902/1‑101 (MQ=255)
                                                         tGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtc                                             >  1:253632/1‑101 (MQ=255)
                                                          ggCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTtct                                            >  2:2324470/1‑101 (MQ=255)
                                                            ccGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTcg                                          >  1:2297993/1‑101 (MQ=255)
                                                            ccGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTcg                                          >  2:1107400/1‑101 (MQ=255)
                                                             cgcgGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCACCGCCCATAGCGGGCGCTTCTcgc                                         >  2:54788/1‑101 (MQ=255)
                                                                   cGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCccgccg                                   >  2:3077769/1‑101 (MQ=255)
                                                                    ggTGACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgg                                  <  1:1745641/101‑1 (MQ=255)
                                                                    ggTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgg                                  >  1:702560/1‑101 (MQ=255)
                                                                     gTGACGCGGCCATGCGAGACATCTGCAGTCCCCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCggg                                 <  2:4078603/101‑1 (MQ=255)
                                                                      tGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgggg                                >  1:3257949/1‑101 (MQ=255)
                                                                      tGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCgggg                                >  1:463059/1‑101 (MQ=255)
                                                                       gccgcgGCCAGGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGc                               <  2:3753614/99‑1 (MQ=255)
                                                                       gACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCCCCCCGCCCATAGCGGGCGCTGCTCGCCCGCCGGGGc                               >  1:4297776/1‑101 (MQ=255)
                                                                        tcgcgCCCTTGCGAGACACCTGCAGTCACCCCCGCCGGGGACCAAAGCCCCCGGCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCa                              <  2:2070708/100‑1 (MQ=18)
                                                                        ccgcgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCa                              <  1:3215903/100‑1 (MQ=255)
                                                                         cgcgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAg                             <  1:4138962/101‑1 (MQ=255)
                                                                         cccgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAg                             <  2:1101384/99‑1 (MQ=255)
                                                                           cgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgc                           >  1:170965/1‑101 (MQ=255)
                                                                           cgGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgc                           >  1:4290684/1‑101 (MQ=255)
                                                                            ggCCAGGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgcg                          <  2:82879/101‑1 (MQ=255)
                                                                            cgCCATGCGCGGCATCTGCGTCCCCCGCCGCCGGGGCCGGAACTCCCCGTCCGGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGgcg                          <  1:631489/100‑1 (MQ=12)
                                                                                aTGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCgg                      >  1:2203778/1‑101 (MQ=255)
                                                                                    gagaCATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctc                  >  1:2606207/1‑101 (MQ=255)
                                                                                     agccATCTGCGGCCACCACCGCGGGGCACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct                 <  2:3289864/98‑1 (MQ=17)
                                                                                     agaCATCTGCAGCCACCACCGCCGGGGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct                 <  2:2951118/101‑1 (MQ=255)
                                                                                     agaCATCAGCTGTCCCCCCCGCCGGGGACTAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGTCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCctct                 <  1:1529184/101‑1 (MQ=18)
                                                                                      gaCATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTg                >  2:2657096/1‑101 (MQ=255)
                                                                                        cATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCGTAGCGGGCGCTTCTCGCCCGCCCGGGCAGGCGCCGGCCTCTGtt              >  2:2178822/1‑101 (MQ=255)
                                                                                          tCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgc            >  2:4413570/1‑101 (MQ=255)
                                                                                           cTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg           >  1:3136007/1‑101 (MQ=255)
                                                                                           cTGCAGCCCCCACCGCCGGGGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcg           <  1:3006180/101‑1 (MQ=255)
                                                                                            tGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgc          >  2:3370012/1‑101 (MQ=255)
                                                                                             gCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcg         >  2:1224451/1‑101 (MQ=255)
                                                                                             gCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcc         >  2:3123860/1‑100 (MQ=255)
                                                                                             gCAGCCCCCCCCGCCGGGGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcg         <  1:3550259/101‑1 (MQ=17)
                                                                                              cAGTCCCCCCCGCCGGGGACCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTgcgcgc        <  1:275913/101‑1 (MQ=255)
                                                                                              cAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGGTgcgcgc        >  2:4293623/1‑101 (MQ=255)
                                                                                               cgTCCCCCCCGCCGGGTACCAAAGCCCCCGNCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg       <  1:54794/100‑1 (MQ=17)
                                                                                               aGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGcgcgcg       >  2:1916288/1‑101 (MQ=255)
                                                                                               aGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGGTGcccgcg       >  1:3450732/1‑101 (MQ=255)
                                                                                               aGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCCCCCATAGCGGGGGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCCCTGTTgcgcggg       >  1:1235042/1‑99 (MQ=255)
                                                                                                 tCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCggg     >  1:1366013/1‑101 (MQ=255)
                                                                                                   accaccGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGc   >  1:3343939/1‑101 (MQ=255)
                                                                                                    ccacgGCCGGGGCCCAAAGCCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCa  <  2:2489984/101‑1 (MQ=255)
                                                                                                    ccaccGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCa  >  2:1632595/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
GCGCAGACGGCCCCGCCTTGGTCTTTTCGGGGGCGCATTCTCTGAAAGCGCCTGACTTGGCCGCGGCCGGTGACGCGGCCATGCGAGACATCTGCAGTCACCACCGCCGGGGACCAAAGTCCCCGGCCAGCGCCCGCCCCGCCCATAGCGGGCGCTTCTCGCCCGCCGGGGCAGGCGCCGGCCTCTGTTGCGCGCGGGGCA  >  NC_014034/2953207‑2953407

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG