Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,959,195 T→C 100% E74E (GAA→GAG RCAP_rcc02771 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,959,1950T→C100.0% 923.3 239E74E (GAA→GAGRCAP_rcc02771TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (133/106):  ref base (0/0):  total (133/106)

Only 100 of 247 total aligned reads displayed.

AGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCG  >  NC_014034/2959097‑2959292
                                                                                                  |                                                                                                 
aGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTc                                                                                                 <  1:938249/101‑1 (MQ=255)
aGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTc                                                                                                 >  2:3122769/1‑101 (MQ=255)
aGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTc                                                                                                 >  2:3357709/1‑101 (MQ=255)
  ccccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                               <  1:2852681/101‑1 (MQ=255)
  ccccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCAt                                                                                               >  2:484538/1‑101 (MQ=255)
   cccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATc                                                                                              >  2:164770/1‑101 (MQ=255)
   cccAGCGGAGAGGCGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGACTCATc                                                                                              >  2:2271582/1‑101 (MQ=255)
    ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCg                                                                                             <  1:1028200/101‑1 (MQ=255)
    ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCg                                                                                             <  1:1455585/101‑1 (MQ=255)
    ccAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCg                                                                                             <  2:4623702/101‑1 (MQ=255)
       gcggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGcc                                                                                          <  2:1090631/101‑1 (MQ=255)
        cggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCt                                                                                         >  2:645531/1‑101 (MQ=255)
        cggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCt                                                                                         <  1:4491280/101‑1 (MQ=255)
        cggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCCCCCGCATTGATGAATTCCAGCGCCTCATCGGCCt                                                                                         <  1:4643140/101‑1 (MQ=255)
         ggcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTg                                                                                        <  2:2958319/101‑1 (MQ=255)
          gcgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGTGCCTCATCGGCCTgg                                                                                       <  1:3931596/101‑1 (MQ=255)
           cgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgc                                                                                      <  1:256299/101‑1 (MQ=255)
           cgAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgc                                                                                      >  2:1421114/1‑101 (MQ=255)
            gAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGgcg                                                                                     >  2:1762139/1‑101 (MQ=255)
              ggTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCt                                                                                   >  1:1602843/1‑101 (MQ=255)
               gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTg                                                                                  <  2:4157497/101‑1 (MQ=255)
               gTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTg                                                                                  >  2:3818389/1‑101 (MQ=255)
                tGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTgg                                                                                 >  1:1059904/1‑101 (MQ=255)
                tGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTgg                                                                                 >  1:4107180/1‑101 (MQ=255)
                tGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTgg                                                                                 >  2:3245894/1‑101 (MQ=255)
                 gATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGc                                                                                >  1:2981214/1‑101 (MQ=255)
                 gATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGc                                                                                >  1:3366566/1‑101 (MQ=255)
                   tGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGTAt                                                                              >  2:782232/1‑101 (MQ=255)
                    gTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAtt                                                                             >  2:3779245/1‑101 (MQ=255)
                    gTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCAtt                                                                             <  1:1855297/101‑1 (MQ=255)
                      aaTCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCg                                                                           >  2:286050/1‑101 (MQ=255)
                      aaTCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCg                                                                           >  1:2768270/1‑101 (MQ=255)
                       aTCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGc                                                                          >  2:697326/1‑101 (MQ=255)
                        tCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGcc                                                                         >  2:3920757/1‑101 (MQ=255)
                         cGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGccc                                                                        >  2:2731472/1‑101 (MQ=255)
                          gATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTcagatc                                                                       >  1:4304902/1‑96 (MQ=255)
                          gATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTTGCCCg                                                                       >  2:1829196/1‑101 (MQ=255)
                           aTCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGt                                                                      >  2:319846/1‑101 (MQ=255)
                             cAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcg                                                                    <  1:1278546/101‑1 (MQ=255)
                              aGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTcgc                                                                   >  2:1707864/1‑101 (MQ=255)
                                cTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGa                                                                 >  2:1141150/1‑101 (MQ=255)
                                cTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCGGGCATTCGCCCGTCGCGa                                                                 >  1:1690848/1‑101 (MQ=255)
                                  tgtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATc                                                               >  2:3114061/1‑101 (MQ=255)
                                  tgtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATc                                                               >  1:559015/1‑101 (MQ=255)
                                  tgtgGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATc                                                               <  1:11009/101‑1 (MQ=255)
                                      gCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCa                                                           <  2:2012744/101‑1 (MQ=255)
                                       cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCAt                                                          <  2:4389777/101‑1 (MQ=255)
                                       cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCAt                                                          <  1:2609953/101‑1 (MQ=255)
                                       cccGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCAt                                                          >  1:3701103/1‑101 (MQ=255)
                                         cGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGa                                                        >  1:3554605/1‑101 (MQ=255)
                                          gCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGaa                                                       <  2:3370905/101‑1 (MQ=255)
                                            aTAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAca                                                     <  2:2659905/101‑1 (MQ=255)
                                            aTAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAca                                                     <  2:558967/101‑1 (MQ=255)
                                              aGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAAcagc                                                   >  1:1400262/1‑101 (MQ=255)
                                                 gcagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACagcagc                                                <  2:2071763/101‑1 (MQ=255)
                                                  cggcaCCTCGCGCACCGGCCTGTCGCCCGCATTGCTGAATCCCAGCACCTCATCGGCCTGGCGCTGGCATTCGCCCGACGCGATCGCCCTGAACCGCAGcc                                               >  2:2484858/3‑101 (MQ=255)
                                                  cagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGcc                                               >  2:416917/1‑101 (MQ=255)
                                                  cagcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGcc                                               <  2:590210/101‑1 (MQ=255)
                                                    gcaCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGc                                             >  2:1960331/1‑101 (MQ=255)
                                                     caCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTGGCCCGTCGCGATCGCCATGAACAGCAGCCGCt                                            >  1:208864/1‑101 (MQ=255)
                                                      cccTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCtt                                           <  2:1135777/100‑1 (MQ=255)
                                                      aCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCtt                                           >  1:2368390/1‑101 (MQ=255)
                                                      aCCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCtt                                           >  2:4010344/1‑101 (MQ=255)
                                                        cTCGCCCCCCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGt                                         <  1:1968913/101‑1 (MQ=255)
                                                            cgcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCgg                                     <  1:4368549/101‑1 (MQ=255)
                                                             gcACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCggg                                    >  2:4298668/1‑101 (MQ=255)
                                                              ccccGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGa                                   <  1:1467145/99‑1 (MQ=255)
                                                               cccGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGaa                                  <  1:866707/100‑1 (MQ=255)
                                                               aCCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGaa                                  >  2:3943484/1‑101 (MQ=255)
                                                               aCCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGaa                                  >  1:418019/1‑101 (MQ=255)
                                                                ccGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGaaa                                 <  1:1829322/101‑1 (MQ=255)
                                                                 cGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAAt                                <  2:849716/101‑1 (MQ=255)
                                                                 cGGCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAAt                                <  1:1141239/101‑1 (MQ=255)
                                                                  ggCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATa                               <  1:1762258/101‑1 (MQ=255)
                                                                   gCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAg                              >  2:4314403/1‑101 (MQ=255)
                                                                   gCCTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAg                              >  2:3569885/1‑101 (MQ=255)
                                                                    ccTGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGTCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGc                             <  2:1605558/101‑1 (MQ=255)
                                                                      tGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCtt                           >  2:1276134/1‑100 (MQ=255)
                                                                      tGTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTg                           >  1:1523019/1‑101 (MQ=255)
                                                                       gTCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGGCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGt                          <  1:378822/101‑1 (MQ=255)
                                                                        tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                         <  2:289871/101‑1 (MQ=255)
                                                                        tCGCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTa                         >  1:2290948/1‑101 (MQ=255)
                                                                          gCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTaga                       >  1:3802305/1‑101 (MQ=255)
                                                                          gCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTaga                       <  2:309332/101‑1 (MQ=255)
                                                                          gCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTaga                       >  1:2225013/1‑101 (MQ=255)
                                                                          gCCCGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTaga                       >  1:1619214/1‑101 (MQ=255)
                                                                           cccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagag                      <  2:3056919/101‑1 (MQ=255)
                                                                            ccGCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTagagc                     <  1:3114305/101‑2 (MQ=255)
                                                                               cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                  <  1:645592/101‑1 (MQ=255)
                                                                               cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                  >  2:3502978/1‑101 (MQ=255)
                                                                                aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGATTGTCCGGGAAATAGCTGTAGAGGGTCg                 >  1:689002/1‑101 (MQ=255)
                                                                                aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCAGCTTGGCCGAGAAACAGCTGTAAAGCGTCg                 >  1:4533172/1‑101 (MQ=255)
                                                                                 ttGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGc                >  1:3660399/1‑101 (MQ=255)
                                                                                  tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc               >  1:3441214/1‑101 (MQ=255)
                                                                                  tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc               >  1:817858/1‑101 (MQ=255)
                                                                                  tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc               <  2:2464320/101‑1 (MQ=255)
                                                                                    atgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCtt             <  2:4520262/101‑1 (MQ=255)
                                                                                        aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac         >  1:3300078/1‑101 (MQ=255)
                                                                                            cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc     >  2:101075/1‑101 (MQ=255)
                                                                                               cgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCg  <  2:2358330/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AGCCCCAGCGGCGAGGTGATGTAATCGATCAGCTTGTGGCCCGCATAGCGCAGCACCTCGCGCACCGGCCTGTCGCCCGCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCG  >  NC_014034/2959097‑2959292

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG