Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,959,273 T→C 100% T48T (ACA→ACG RCAP_rcc02771 ← TetR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,959,2730T→C100.0% 843.5 224T48T (ACA→ACGRCAP_rcc02771TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (105/118):  ref base (0/1):  total (105/119)

Only 100 of 240 total aligned reads displayed.

GCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCT  >  NC_014034/2959175‑2959370
                                                                                                  |                                                                                                 
gCATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAgcgt                                                                                                 >  1:3715267/1‑101 (MQ=255)
 cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTc                                                                                                >  1:2265848/1‑101 (MQ=255)
 cATTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGATGTAGAgcgtg                                                                                                >  2:3494450/1‑100 (MQ=255)
  aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCg                                                                                               >  1:175297/1‑101 (MQ=255)
  aTTGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCAGCTTGGCCGAGAAACAGCTGTAAAGCGTCg                                                                                               >  1:4533172/1‑101 (MQ=255)
   ttGATGAATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGc                                                                                              >  2:3926622/1‑101 (MQ=255)
    tgatgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGcc                                                                                             <  2:2464320/101‑1 (MQ=255)
      atgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCtt                                                                                           <  2:4520262/101‑1 (MQ=255)
       tgaATTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTg                                                                                          >  2:454318/1‑101 (MQ=255)
          aTTCCAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGac                                                                                       >  1:3300078/1‑101 (MQ=255)
             ccAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAcc                                                                                    >  1:1850346/1‑101 (MQ=255)
              cAGCGCCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccc                                                                                   >  2:3240312/1‑101 (MQ=255)
                gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc                                                                                 <  2:1825108/101‑1 (MQ=255)
                gcgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACAccccc                                                                                 <  1:3186717/101‑1 (MQ=255)
                 cgcCTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCg                                                                                <  2:2358330/101‑1 (MQ=255)
                  gcctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGc                                                                               >  2:3638249/4‑101 (MQ=255)
                    ctCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTAGCCTTGGACGCCCCCGCCt                                                                             <  1:3494708/100‑1 (MQ=255)
                     tCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTc                                                                            <  1:440910/101‑1 (MQ=255)
                       aTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgc                                                                          >  2:3413436/1‑101 (MQ=255)
                       aTCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTccc                                                                          >  1:2232177/1‑99 (MQ=255)
                        tCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTcgcg                                                                         <  1:1659002/101‑1 (MQ=255)
                               tgcgcTGGCATTCGCCCGTCGCGATCGCCATGAACGGCAGCCGCTTGTCCGGGAAATACCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATg                                                                  <  2:2380014/100‑1 (MQ=255)
                               ggCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATg                                                                  >  1:3442944/1‑101 (MQ=255)
                                gcgcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGt                                                                 <  1:2072768/101‑1 (MQ=255)
                                 cgcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTc                                                                <  2:110972/101‑1 (MQ=255)
                                 cgcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTc                                                                <  1:2382645/101‑1 (MQ=255)
                                  gcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCa                                                               >  1:744044/1‑101 (MQ=255)
                                  gcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCa                                                               >  2:2961101/1‑101 (MQ=255)
                                  gcTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCa                                                               <  2:580257/101‑1 (MQ=255)
                                   cTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCAt                                                              <  1:1243072/101‑1 (MQ=255)
                                    tGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATc                                                             >  1:1400448/1‑101 (MQ=255)
                                    tGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATc                                                             <  2:3453968/101‑1 (MQ=255)
                                       cATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAc                                                          <  1:1504125/101‑1 (MQ=255)
                                        aTTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAcc                                                         >  2:102870/1‑101 (MQ=255)
                                        aTTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAcc                                                         >  2:3292297/1‑101 (MQ=255)
                                        aTTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGAcc                                                         >  1:158991/1‑101 (MQ=255)
                                         ttCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCg                                                        >  2:1677882/1‑101 (MQ=255)
                                         ttCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCg                                                        <  1:3075300/101‑1 (MQ=255)
                                          tCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGa                                                       >  1:2384547/1‑101 (MQ=255)
                                             cccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgc                                                    <  1:3163531/101‑1 (MQ=255)
                                             cccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgc                                                    <  2:3554347/101‑1 (MQ=255)
                                             cccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgc                                                    >  2:194857/1‑101 (MQ=255)
                                              ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   <  2:1400167/101‑1 (MQ=255)
                                              ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   >  2:1363839/1‑101 (MQ=255)
                                              ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   >  2:3760678/1‑101 (MQ=255)
                                              ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   >  2:2686210/1‑101 (MQ=255)
                                              ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   >  1:2875579/1‑101 (MQ=255)
                                              ccGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcg                                                   <  2:2768072/101‑1 (MQ=255)
                                               cGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAgcgc                                                  >  1:4643223/1‑101 (MQ=255)
                                                gTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGcc                                                 >  2:3978669/1‑101 (MQ=255)
                                                  cgcgATCGCCATGCACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCt                                               <  1:4010629/101‑1 (MQ=255)
                                                  cgcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCt                                               <  1:3943769/101‑1 (MQ=255)
                                                   gcgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTc                                              <  1:3640949/101‑1 (MQ=255)
                                                    cgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCg                                             <  1:706007/101‑1 (MQ=255)
                                                    cgATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATAGACCGAAGCGCCCTCg                                             >  2:2838637/1‑101 (MQ=255)
                                                     gATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGa                                            <  1:929936/101‑1 (MQ=255)
                                                     gATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGa                                            <  1:4243176/101‑1 (MQ=255)
                                                         gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        <  2:4106894/101‑1 (MQ=255)
                                                         gCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAAccc                                        <  1:2578643/101‑1 (MQ=255)
                                                          ccATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCg                                       <  2:827189/101‑1 (MQ=255)
                                                          ccATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCg                                       >  1:1360973/1‑101 (MQ=255)
                                                            aTGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTc                                     >  2:811106/1‑101 (MQ=255)
                                                              gAAGAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgc                                   <  2:688940/101‑1 (MQ=255)
                                                              gAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgc                                   >  2:1226412/1‑101 (MQ=255)
                                                              gAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgc                                   >  2:4115959/1‑101 (MQ=255)
                                                               aaCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACCCCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgcg                                  <  2:316693/101‑1 (MQ=255)
                                                               aaCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTcgcg                                  >  2:3743952/1‑101 (MQ=255)
                                                                aCAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCgcgc                                 <  2:3392000/101‑1 (MQ=255)
                                                                 cagcagCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCa                                <  1:2731670/101‑1 (MQ=255)
                                                                 cagcagCCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCa                                <  1:3818674/101‑1 (MQ=255)
                                                                   gcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGa                              >  1:2791029/1‑101 (MQ=255)
                                                                   gcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGa                              <  1:2706710/101‑1 (MQ=255)
                                                                   gcagcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTAGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGa                              <  2:298098/101‑1 (MQ=255)
                                                                     agcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGaaa                            <  1:2515238/101‑1 (MQ=255)
                                                                      gcCGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAac                           >  2:4229413/1‑101 (MQ=255)
                                                                        cGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAacac                         <  1:2961330/101‑1 (MQ=255)
                                                                        cGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAacac                         <  2:1059815/101‑1 (MQ=255)
                                                                        cGCTTGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAacac                         >  1:1881447/1‑101 (MQ=255)
                                                                           ttGTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCTCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACttt                      <  2:3714992/101‑1 (MQ=255)
                                                                             gTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTcc                    >  2:1850168/1‑101 (MQ=255)
                                                                             gTCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTcc                    <  1:1282449/101‑1 (MQ=255)
                                                                              tCCGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcg                   <  2:3466065/101‑1 (MQ=255)
                                                                               ccGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcgc                  >  1:847769/1‑101 (MQ=255)
                                                                                cGGGAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCcgcg                 <  1:3413690/101‑1 (MQ=255)
                                                                                   gAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCgcgcgc              >  1:1899992/1‑101 (MQ=255)
                                                                                   gAAATAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCgcgcgc              >  1:3202655/1‑101 (MQ=255)
                                                                                    aaaTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGcc             >  1:107778/1‑101 (MQ=255)
                                                                                     aaTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGTTGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCg            <  1:63333/101‑1 (MQ=255)
                                                                                      aTAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGt           <  2:1266568/101‑1 (MQ=255)
                                                                                       tAGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTc          >  2:2257349/1‑101 (MQ=255)
                                                                                        aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         <  2:2259228/101‑1 (MQ=255)
                                                                                        aGCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCa         >  1:478590/1‑101 (MQ=255)
                                                                                         gCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCaa        >  2:2067121/1‑101 (MQ=255)
                                                                                         gCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCaa        <  1:416954/101‑1 (MQ=255)
                                                                                         gCTGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCaa        <  2:743969/101‑1 (MQ=255)
                                                                                           tGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAgg      <  1:789531/101‑1 (MQ=255)
                                                                                           tGTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAgg      <  1:3110483/101‑1 (MQ=255)
                                                                                            gTAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGa     >  1:3363410/1‑101 (MQ=255)
                                                                                             tAGAGCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGAc    >  1:4250468/1‑101 (MQ=255)
                                                                                              agagCGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGAcc   >  1:1189438/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCATTGATGAATTCCAGCGCTTCATCGGCCTGGCGCTGGCATTCGCCCGTCGCGATCGCCATGAACAGCAGCCGCTTGTCCGGGAAATAGCTGTAGAGTGTCGCCTTGGACACCCCCGCCTCGCGCGCGATGTCATCGACCGAAGCGCCCTCGAACCCGTCGCGCAGAAACACTTTCCGCGCGCCGTCAAGGACCT  >  NC_014034/2959175‑2959370

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG