Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,960,586 A→G 100% K152K (AAA→AAG hisP → polar amino acid ABC transporter ATP‑binding protein HisP

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,960,5860A→G100.0% 854.4 227K152K (AAA→AAGhisPpolar amino acid ABC transporter ATP‑binding protein HisP
Reads supporting (aligned to +/- strand):  new base (113/113):  ref base (0/0):  total (113/114)

Only 100 of 231 total aligned reads displayed.

ATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960489‑2960683
                                                                                                 |                                                                                                 
aTGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGcc                                                                                                <  1:3711874/101‑1 (MQ=255)
 tGTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               <  1:4303725/101‑1 (MQ=255)
 aggcATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCg                                                                                               <  2:1301609/98‑1 (MQ=255)
  gTCATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGa                                                                                              >  1:3229861/1‑101 (MQ=255)
    cATGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACg                                                                                            <  2:2262758/101‑1 (MQ=255)
     aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  1:494059/1‑101 (MQ=255)
     aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  1:4610458/1‑101 (MQ=255)
     aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           <  2:425166/101‑1 (MQ=255)
     aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  2:2872750/1‑101 (MQ=255)
     aTGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGc                                                                                           >  2:2565774/1‑101 (MQ=255)
      tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                          <  2:4180488/101‑1 (MQ=255)
      tGGAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGACAAGGTCGGCATCGGCGACAAGGCCGACGcc                                                                                          >  1:3450177/1‑101 (MQ=255)
       ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                                                                         <  1:2872966/101‑1 (MQ=255)
       ggAGGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCt                                                                                         <  1:1968613/101‑1 (MQ=255)
            cgcCGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGccc                                                                                    <  2:1675377/101‑1 (MQ=255)
               cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGACTGGCCcgcg                                                                                 >  2:1877139/1‑101 (MQ=255)
               cGGTGACGGTTCTGGGCCGCAAACCGGCCGAGGCCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCcgcg                                                                                 >  2:2317439/1‑101 (MQ=255)
                ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                                                                                <  1:4316713/101‑1 (MQ=255)
                ggTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCgcgc                                                                                >  1:1892470/1‑101 (MQ=255)
                 gTGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCa                                                                               >  1:4519265/1‑101 (MQ=255)
                  tGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAg                                                                              <  1:2540294/101‑1 (MQ=255)
                  tGACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGAAGCCTTCTGGCCAAGGTCGGCATCGGCTACAAGGCCGACGCCTGGCCGCCGCac                                                                              >  2:796446/1‑100 (MQ=255)
                   gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             <  1:4236695/101‑1 (MQ=255)
                   gACGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGc                                                                             >  2:2733571/1‑101 (MQ=255)
                    aCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                                                                            >  1:3040875/1‑101 (MQ=255)
                    aCGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGct                                                                            <  1:2570007/101‑1 (MQ=255)
                     cGGTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctc                                                                           >  1:4202557/1‑101 (MQ=255)
                      ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                                                                          <  1:713531/101‑1 (MQ=255)
                      ggTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGctct                                                                          >  1:1018874/1‑101 (MQ=255)
                       gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                         >  1:24027/1‑101 (MQ=255)
                       gTTCTGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCtctc                                                                         >  2:2075352/1‑101 (MQ=255)
                           tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     <  2:1314922/101‑1 (MQ=255)
                           tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  1:241566/1‑101 (MQ=255)
                           tGGGCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggc                                                                     >  2:812255/1‑101 (MQ=255)
                            gggCCGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGggcg                                                                    >  2:3646318/1‑101 (MQ=255)
                                cGCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCca                                                                >  2:4468117/1‑101 (MQ=255)
                                 gCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag                                                               <  2:628728/101‑1 (MQ=255)
                                 gCAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcag                                                               <  2:494028/101‑1 (MQ=255)
                                  cAAACCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagc                                                              >  2:4300555/1‑101 (MQ=255)
                                   aaaCCGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCcagca                                                             <  1:1488437/101‑1 (MQ=255)
                                       cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa                                                         >  2:2703078/1‑101 (MQ=255)
                                       cGGCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCaa                                                         <  1:3176104/101‑1 (MQ=255)
                                        ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  2:568472/101‑1 (MQ=255)
                                        ggCCGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAc                                                        <  1:3058259/101‑1 (MQ=255)
                                           cGAGGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     >  1:2694873/1‑101 (MQ=255)
                                           cGAGGTCCAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAAcgcg                                                     <  2:2096641/101‑1 (MQ=255)
                                            gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                    <  2:3835701/98‑1 (MQ=255)
                                            gaggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACgcgc                                                    <  2:3415647/98‑1 (MQ=255)
                                             aggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgcc                                                   <  2:2977306/99‑1 (MQ=255)
                                              ggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccg                                                  <  1:4667924/100‑1 (MQ=255)
                                              ggTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGcgccg                                                  >  1:667527/2‑101 (MQ=255)
                                                tCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCgccgcc                                                <  2:1387459/101‑1 (MQ=255)
                                                 cGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCa                                               >  1:4331378/1‑101 (MQ=255)
                                                  gAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCAt                                              >  1:3653405/1‑101 (MQ=255)
                                                   aaCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATc                                             >  2:2479294/1‑101 (MQ=255)
                                                   aaCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATc                                             <  2:1265906/101‑1 (MQ=255)
                                                   aaCCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATc                                             >  1:3386242/1‑101 (MQ=255)
                                                     ccGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGc                                           <  2:1602432/101‑1 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                         <  2:4403386/101‑1 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                         <  1:596554/101‑1 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                         >  2:59472/1‑101 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                         >  1:528523/1‑101 (MQ=255)
                                                       gCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCGCGGGCGGCCAGCAGCAACGCGCCGCCATCGccc                                         >  1:3974535/1‑101 (MQ=255)
                                                        cTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcg                                        >  2:2398252/1‑101 (MQ=255)
                                                        cTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcg                                        >  2:875976/1‑101 (MQ=255)
                                                           gCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCcgcgc                                     >  1:3801579/1‑101 (MQ=255)
                                                                cAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGctct                                >  2:2765519/1‑101 (MQ=255)
                                                                 aGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtg                               >  1:1393771/1‑101 (MQ=255)
                                                                 aGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtg                               >  1:2324538/1‑101 (MQ=255)
                                                                 aGCCTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtg                               <  1:3600973/101‑1 (MQ=255)
                                                                   ccTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgca                             <  1:3346198/101‑1 (MQ=255)
                                                                    cTTCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcat                            <  2:1101751/101‑1 (MQ=255)
                                                                      tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                          >  1:1149367/1‑101 (MQ=255)
                                                                      tCTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgc                          <  1:1982085/101‑1 (MQ=255)
                                                                       cTGGCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                         >  2:3729710/1‑101 (MQ=255)
                                                                       cTGGCCAAGGTCGGCATCGGCGACAAGCCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCtgcatgca                         <  1:2009371/101‑1 (MQ=255)
                                                                         ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  2:2373293/101‑1 (MQ=255)
                                                                         ggCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGc                       <  2:3001507/101‑1 (MQ=255)
                                                                          gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      >  2:2174746/1‑101 (MQ=255)
                                                                          gCCAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGcc                      >  2:36955/1‑101 (MQ=255)
                                                                           ccAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCg                     >  1:298258/1‑101 (MQ=255)
                                                                            cAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    <  1:179830/101‑1 (MQ=255)
                                                                            cAAGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    >  1:2220485/1‑101 (MQ=255)
                                                                            cAAAGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGc                    >  2:157925/1‑101 (MQ=255)
                                                                              aGGTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAg                  <  1:2733769/101‑1 (MQ=255)
                                                                               ggTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAgg                 >  1:3113132/1‑101 (MQ=255)
                                                                                gTCGGCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGgc                <  2:243322/101‑1 (MQ=255)
                                                                                   ggCATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCt             >  1:879457/1‑101 (MQ=255)
                                                                                     cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCTCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           >  1:3879316/1‑101 (MQ=255)
                                                                                     cATCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCttt           <  2:3229616/101‑1 (MQ=255)
                                                                                      aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt          <  2:30344/101‑1 (MQ=255)
                                                                                      aTCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCtttt          >  2:1954761/1‑101 (MQ=255)
                                                                                       tCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTg         <  2:3739573/101‑1 (MQ=255)
                                                                                       tCGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTg         >  1:224183/1‑101 (MQ=255)
                                                                                        cGGCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGt        <  2:4006665/101‑1 (MQ=255)
                                                                                          gCGACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTc      <  1:1697938/101‑1 (MQ=255)
                                                                                           ngACAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTNTGTTng     >  1:315666/2‑101 (MQ=255)
                                                                                             ncNNNGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCCCTGCATGCAGCCGCAGGCGCTTTTGTTcgac   <  1:4131624/100‑1 (MQ=255)
                                                                                              cAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgacg  >  2:3599969/1‑101 (MQ=255)
                                                                                              cAAGGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTcgacg  <  2:2957261/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
ATGTCATGGAAGCGCCGGTGACGGTTCTGGGCCGCAAACCGGCCGAAGTCGAACCGCTCGCCCGCAGCCTTCTGGCCAAGGTCGGCATCGGCGACAAAGCCGACGCCTGGCCCGCGCAGCTCTCGGGCGGCCAGCAGCAACGCGCCGCCATCGCCCGCGCGCTCTGCATGCAGCCGCAGGCGCTTTTGTTCGACG  >  NC_014034/2960489‑2960683

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG