BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,962,711	A→G	100%	K21E (AAG→GAG)	hisM →	polar amino acid ABC transporter permease HisM

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,962,711	0	A→G	100.0%	851.9	225	K21E (AAG→GAG)	hisM	polar amino acid ABC transporter permease HisM
Reads supporting (aligned to +/- strand): new base (97/128): ref base (0/0): total (97/128)

Only 100 of 236 total aligned reads displayed.


GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808


gCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAg                                                                                                 <  2:2635467/101‑1 (MQ=255)


 cgacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgc                                                                                                <  1:3860612/101‑1 (MQ=255)


  gacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgcg                                                                                               <  2:243846/101‑1 (MQ=255)


  gacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAgcg                                                                                               >  2:2797494/1‑101 (MQ=255)


  gacgaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCCATGGCCAgcg                                                                                               >  2:2095879/1‑101 (MQ=255)


     gaTGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGt                                                                                            <  2:2145868/101‑1 (MQ=255)


       tGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgc                                                                                          <  1:983057/101‑1 (MQ=255)


       tGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgc                                                                                          >  2:4231021/1‑101 (MQ=255)


        ggcgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgct                                                                                         >  2:2912008/1‑101 (MQ=255)


        ggcgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgct                                                                                         <  2:4388800/101‑1 (MQ=255)


          cgggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGtgctgc                                                                                       <  2:2034215/101‑1 (MQ=255)


           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      >  1:2231214/1‑101 (MQ=255)


           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      >  1:2208097/1‑101 (MQ=255)


           gggcgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGcc                                                                                      <  2:4493601/101‑1 (MQ=255)


              cgAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcgcg                                                                                   <  1:1392673/101‑1 (MQ=255)


                aaGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCa                                                                                 <  1:522889/101‑1 (MQ=255)


                  gcgcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCcgctacaa                                                                               <  2:717644/101‑6 (MQ=255)


                   ttccgatctaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTg                                                                              <  2:38631/92‑1 (MQ=255)


                   cgcAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTg                                                                              <  2:1481323/101‑1 (MQ=255)


                      accGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           <  1:1842798/99‑1 (MQ=255)


                      aaCGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACt                                                                           <  1:626889/101‑1 (MQ=255)


                        cGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTc                                                                         >  1:2059422/1‑101 (MQ=255)


                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       <  1:2386525/101‑1 (MQ=255)


                          gAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCAc                                                                       >  1:1437192/1‑101 (MQ=255)


                           aaGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACg                                                                      <  2:2589514/101‑1 (MQ=255)


                              gCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGctc                                                                   >  2:3704362/1‑101 (MQ=255)


                               ccGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGctct                                                                  <  2:1262682/101‑1 (MQ=255)


                                cgcgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTg                                                                 <  1:1696950/101‑1 (MQ=255)


                                 gcgGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGc                                                                <  1:4534381/101‑1 (MQ=255)


                                   ggCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCa                                                              >  1:4250904/1‑101 (MQ=255)


                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             <  2:1249137/101‑1 (MQ=255)


                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             <  1:716987/101‑1 (MQ=255)


                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             >  1:2638555/1‑101 (MQ=255)


                                    gCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCAt                                                             >  2:3349509/1‑101 (MQ=255)


                                      aTGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGa                                                           <  1:3209046/101‑1 (MQ=255)


                                      aTGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGa                                                           >  1:85433/1‑101 (MQ=255)


                                       tGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGaa                                                          <  1:3396152/101‑1 (MQ=255)


                                        gAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAAt                                                         <  2:234457/101‑1 (MQ=255)


                                          gCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTc                                                       <  1:3456616/101‑1 (MQ=255)


                                           cGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCa                                                      <  1:1796398/101‑1 (MQ=255)


                                            gACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCAc                                                     >  2:3199520/1‑101 (MQ=255)


                                               aGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCt                                                  >  1:1275586/1‑101 (MQ=255)


                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 <  1:2306984/101‑1 (MQ=255)


                                                gCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGAGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTg                                                 >  2:1018557/1‑101 (MQ=255)


                                                 cTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGa                                                >  2:4264649/1‑101 (MQ=255)


                                                  tGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGAt                                               >  2:1929172/1‑101 (MQ=255)


                                                  tGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGAt                                               >  1:2899731/1‑101 (MQ=255)


                                                  ggCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGAt                                               <  2:3821432/100‑1 (MQ=255)


                                                   gCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATc                                              >  2:3928393/1‑101 (MQ=255)


                                                    cttccgatctatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  1:3745101/91‑1 (MQ=255)


                                                    cACGCAGATCATCGCGGATTACGCCTTCCGTCCGCTGGCCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCCCGCTCTGCCATGAATTCACCCTGATCg                                             >  1:3882341/1‑101 (MQ=255)


                                                    cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  2:1383699/101‑1 (MQ=255)


                                                    cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             <  1:2912237/101‑1 (MQ=255)


                                                    cACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCg                                             >  1:3761310/1‑101 (MQ=255)


                                                      cGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGc                                           <  2:2478226/101‑1 (MQ=255)


                                                       gCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCt                                          <  2:2013523/101‑1 (MQ=255)


                                                         aGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCg                                        >  1:2513338/1‑101 (MQ=255)


                                                          gATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCgg                                       <  2:3997795/101‑1 (MQ=255)


                                                            tcatcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCaggg                                     >  1:4388484/1‑101 (MQ=255)


                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    <  2:2859404/101‑1 (MQ=255)


                                                             catcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGc                                    >  2:3014065/1‑101 (MQ=255)


                                                              atcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGcg                                   >  2:2109149/1‑100 (MQ=255)


                                                              atcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCt                                   <  1:296476/101‑1 (MQ=255)


                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  2:1681899/101‑1 (MQ=255)


                                                               tcGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTg                                  <  2:678624/101‑1 (MQ=255)


                                                                  cgGATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCCGGGCTGATc                               >  2:2724247/1‑101 (MQ=255)


                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              <  2:3511052/101‑1 (MQ=255)


                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCt                              <  1:2976826/101‑1 (MQ=255)


                                                                   ggATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCCGATCt                              >  2:4212584/1‑101 (MQ=255)


                                                                    gATTACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTg                             >  2:3289205/1‑101 (MQ=255)


                                                                      ttACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGa                           <  1:2447809/101‑1 (MQ=255)


                                                                      ttACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGa                           <  1:404304/101‑1 (MQ=255)


                                                                      ttACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGa                           <  2:493111/101‑1 (MQ=255)


                                                                       tACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTggga                          >  1:3712149/1‑99 (MQ=255)


                                                                       tACGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGaa                          <  1:790049/101‑1 (MQ=255)


                                                                        aCGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAc                         <  1:1080218/101‑1 (MQ=255)


                                                                        aCGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAc                         <  1:1888457/101‑1 (MQ=255)


                                                                        aCGCCTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAAc                         >  1:282769/1‑101 (MQ=255)


                                                                          gCCTTCCGCTCTCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCCGATCTGGAACGt                       >  2:3456382/1‑101 (MQ=255)


                                                                           ccTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTc                      <  1:490454/101‑1 (MQ=255)


                                                                            cTTCCGCTCGGTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     <  2:618462/101‑1 (MQ=255)


                                                                            cTTCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCt                     >  2:1691261/1‑101 (MQ=255)


                                                                             ttCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTa                    <  2:85419/101‑1 (MQ=255)


                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATTTTGAACGTCTAt                   >  1:1209821/1‑101 (MQ=255)


                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGGACGTCTAt                   >  2:2292750/1‑101 (MQ=255)


                                                                              tCCGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAt                   <  2:3982608/101‑1 (MQ=255)


                                                                               ccGCTCGCTGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTAtt                  >  1:2473859/1‑101 (MQ=255)


                                                                                 gctcgcgGGGCTATAGCGAGCGGGTGCTGACGCGCCGTGACTTCACGCGCTGCTATGAATTCACCCTGATCGGCTCGAGGCTGATCTGGAACGTCTATTTc                <  2:782092/101‑1 (MQ=255)


                                                                                  ctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCg               <  1:1976635/101‑1 (MQ=255)


                                                                                  ctcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCg               <  2:1856734/101‑1 (MQ=255)


                                                                                   tcgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCgg              >  1:4191544/1‑101 (MQ=255)


                                                                                    cgctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGc             >  2:2206241/1‑101 (MQ=255)


                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCa            <  2:1157109/101‑1 (MQ=255)


                                                                                     gctGGGCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCa            <  1:1597533/101‑1 (MQ=255)


                                                                                        gggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGc         <  2:2753743/101‑1 (MQ=255)


                                                                                         ggCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCt        <  1:3041656/101‑1 (MQ=255)


                                                                                          gCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTg       >  2:1556855/1‑101 (MQ=255)


                                                                                          gCTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTg       <  2:3006216/101‑1 (MQ=255)


                                                                                           cTATGGCGAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTgg      >  2:1817901/1‑101 (MQ=255)


                                                                                             aTGGCGAGCGCGTGCTGCCGCCCAGTGACTTCGCGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGgcg    <  2:1437097/101‑1 (MQ=255)


GCGACGATGGCGGGCGAAGCGCAACGGAAGGCCGCGGCATGAGCGACAGCTGCACGCAGATCATCGCGGATTACGCCTTCCGCTCGCTGGGCTATGGCAAGCGCGTGCTGCCGCGCAGTGACTTCACGCTCTGCCATGAATTCACCCTGATCGGCTCGGGGCTGATCTGGAACGTCTATTTCGGCACGCTGGCGCT  >  NC_014034/2962613‑2962808

Base quality scores:


ATCG

< 3 ≤


ATCG

< 22 ≤


ATCG

< 30 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG