Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,973,819 T→A 100% V311D (GTT→GAT)  RCAP_rcc02786 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,973,8190T→A100.0% 880.2 226V311D (GTT→GAT) RCAP_rcc02786hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (112/114):  ref base (0/0):  total (112/114)

Only 100 of 233 total aligned reads displayed.

CCTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATACC  >  NC_014034/2973721‑2973916
                                                                                                  |                                                                                                 
ccTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATc                                                                                                 <  1:2126857/101‑1 (MQ=255)
       aaTGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGgcgc                                                                                          >  2:2122862/1‑101 (MQ=255)
       aaTGTCGGCGGCACCATCGACGGGGCGGGGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTgggggg                                                                                          >  2:1543625/1‑98 (MQ=255)
          gTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCggg                                                                                       >  1:4477/1‑101 (MQ=255)
          gTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCggg                                                                                       <  2:3372709/101‑1 (MQ=255)
           tCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCgggg                                                                                      <  1:392496/101‑1 (MQ=255)
              gcggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGcc                                                                                   >  1:677584/1‑101 (MQ=255)
               cggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCg                                                                                  >  1:3165332/1‑101 (MQ=255)
                ggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGa                                                                                 >  2:249498/1‑101 (MQ=255)
                ggcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGa                                                                                 <  2:2688242/101‑1 (MQ=255)
                 gcACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGAt                                                                                <  1:643867/101‑1 (MQ=255)
                    ccATCGACGGGGCGGTGCAGATGGGCGACGGGGCGGAGCTGCTGTTCAATTCGGGCGGGCGGGTCCTTGGCGAGGTCGATCGGGGCGCGGGGGCCGATGGc                                                                             >  1:614175/1‑101 (MQ=255)
                    ccATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGc                                                                             >  1:3432671/1‑101 (MQ=255)
                     cATCGACGGGGCGGTGCTGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCt                                                                            <  2:4194697/101‑1 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           <  2:818636/101‑1 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           <  2:2377250/101‑1 (MQ=255)
                      aTCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTa                                                                           <  1:1832446/101‑1 (MQ=255)
                        cGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacg                                                                         <  1:1009646/101‑1 (MQ=255)
                          aCGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTacgac                                                                       <  1:82162/101‑1 (MQ=255)
                           cGGGGCGGTGTAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATTGCTacgccg                                                                      >  2:3766591/1‑101 (MQ=255)
                            ggggCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACgg                                                                     >  2:1210720/1‑101 (MQ=255)
                            ggggCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACgg                                                                     <  2:2300281/101‑1 (MQ=255)
                                    gCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcg                                                             <  2:130104/101‑1 (MQ=255)
                                     cAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcgg                                                            >  1:2375163/1‑101 (MQ=255)
                                      aGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggc                                                           <  1:963949/101‑1 (MQ=255)
                                       gATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAgcggcg                                                          <  2:4081606/101‑1 (MQ=255)
                                           ggCGACGGGGCCGATCTGCTTATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCt                                                      >  1:1499/1‑101 (MQ=255)
                                           ggCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCt                                                      >  1:2170056/1‑101 (MQ=255)
                                            gCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTg                                                     <  1:4235982/101‑1 (MQ=255)
                                            gCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTg                                                     <  1:1286667/101‑1 (MQ=255)
                                             cGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTgg                                                    >  1:1952900/1‑101 (MQ=255)
                                              gACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGa                                                   >  1:4120411/1‑101 (MQ=255)
                                               gcGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  <  2:3032753/100‑1 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  >  2:2877962/1‑101 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  >  1:302990/1‑101 (MQ=255)
                                               aCGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGAt                                                  <  1:220737/101‑1 (MQ=255)
                                                 ggggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGGGCAGCGGCGCGCTGGATgg                                                >  2:4336987/1‑101 (MQ=255)
                                                 ggggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATgg                                                <  1:3287552/101‑1 (MQ=255)
                                                 ggggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATgg                                                >  2:2702366/1‑101 (MQ=255)
                                                   ggCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCa                                              <  1:262898/101‑1 (MQ=255)
                                                     ccGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGc                                            <  1:2748199/101‑1 (MQ=255)
                                                      cGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCg                                           <  2:1705079/101‑1 (MQ=255)
                                                      cGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCg                                           <  1:1210812/101‑1 (MQ=255)
                                                       gATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTTGATGGCAGCGt                                          <  2:3390952/101‑1 (MQ=255)
                                                        gtCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         <  2:1059442/100‑1 (MQ=255)
                                                        aTCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTc                                         <  2:2204666/101‑1 (MQ=255)
                                                         tCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGTTGCCACCGTCg                                        >  1:4111759/1‑101 (MQ=255)
                                                         tCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCg                                        >  1:2825667/1‑101 (MQ=255)
                                                         tCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCg                                        <  2:3290877/101‑1 (MQ=255)
                                                           tgctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGAt                                      >  1:71676/1‑101 (MQ=255)
                                                           tgctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGAt                                      <  1:2438755/101‑1 (MQ=255)
                                                            gctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGCTg                                     >  2:4667010/1‑101 (MQ=255)
                                                             ctgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATgg                                    <  1:1462581/101‑1 (MQ=255)
                                                              tgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggc                                   <  2:1511882/101‑1 (MQ=255)
                                                              tgATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggc                                   >  2:897540/1‑101 (MQ=255)
                                                               gATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcg                                  <  2:4232278/101‑1 (MQ=255)
                                                               gATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcg                                  >  1:806989/1‑101 (MQ=255)
                                                                 tCAATTCGGGCGGGCTGAGCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGCCGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATggcggc                                <  2:3313894/101‑1 (MQ=255)
                                                                  cAATTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGgcggcg                               >  1:287903/1‑101 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCt                             <  2:677522/101‑1 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCt                             <  2:1755751/101‑1 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCt                             <  1:2949506/101‑1 (MQ=255)
                                                                    aTTCGGGCGGGCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCt                             >  1:2799034/1‑101 (MQ=255)
                                                                       cgggcgggCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgat                          <  1:3675529/101‑1 (MQ=255)
                                                                       cgggcgggCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgat                          >  1:3864583/1‑101 (MQ=255)
                                                                       cgggcgggCGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgat                          >  1:2619615/1‑101 (MQ=255)
                                                                        gggcgggcGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgatg                         <  1:2047611/101‑1 (MQ=255)
                                                                        gggcgggcGGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgatg                         <  2:776995/101‑1 (MQ=255)
                                                                         ggcgggcgGATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCtgatga                        <  2:2532984/101‑1 (MQ=255)
                                                                           cgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacg                      <  1:4589599/101‑1 (MQ=255)
                                                                           cgggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacg                      <  1:3360186/101‑1 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     <  1:1702608/101‑1 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  2:1990718/1‑101 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  1:312578/1‑101 (MQ=255)
                                                                            gggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacga                     >  2:2719220/1‑101 (MQ=255)
                                                                             ggcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATgacgac                    >  2:477757/1‑101 (MQ=255)
                                                                              gcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGaca                   <  2:2799116/101‑1 (MQ=255)
                                                                              gcggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGaca                   <  1:4519064/101‑1 (MQ=255)
                                                                               cggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGacac                  <  2:369431/101‑1 (MQ=255)
                                                                                ggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACAcc                 <  2:3482895/101‑1 (MQ=255)
                                                                                ggATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACAcc                 >  1:230832/1‑101 (MQ=255)
                                                                                 gATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGAGGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCt                >  2:4561347/1‑101 (MQ=255)
                                                                                 gATCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCt                >  1:2541265/1‑101 (MQ=255)
                                                                                  aTCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCtt               <  2:637792/101‑1 (MQ=255)
                                                                                   tCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTc              <  2:2772990/101‑1 (MQ=255)
                                                                                   tCCTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTc              <  2:1980227/101‑1 (MQ=255)
                                                                                    ccTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCa             <  1:3459697/101‑1 (MQ=255)
                                                                                    ccTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCa             <  2:4474/101‑1 (MQ=255)
                                                                                    ccTTGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCa             <  2:2827608/101‑1 (MQ=255)
                                                                                      ttGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACAGCTTCATc           <  1:3571348/101‑1 (MQ=255)
                                                                                       tGGCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCg          >  1:3729094/1‑101 (MQ=255)
                                                                                         gCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGc        <  2:693660/101‑1 (MQ=255)
                                                                                         gCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGc        <  2:4413161/101‑1 (MQ=255)
                                                                                         gCGATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGc        >  1:1250608/1‑101 (MQ=255)
                                                                                           gATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCaa      <  2:3219978/101‑1 (MQ=255)
                                                                                           gATGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCaa      <  1:3774058/101‑1 (MQ=255)
                                                                                            aTGTCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAAt     <  2:1853555/101‑1 (MQ=255)
                                                                                               tCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAcc  >  2:3602503/1‑101 (MQ=255)
                                                                                               tCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAcc  <  2:2606857/101‑1 (MQ=255)
                                                                                               tCGATCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATAcc  <  2:2968422/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCTGCGCAATGTCGGCGGCACCATCGACGGGGCGGTGCAGATGGGCGACGGGGCCGATCTGCTGATCAATTCGGGCGGGCGGATCCTTGGCGATGTCGTTCTGGGCGCGGGGGCCGATCGCTACGACGGGCGCAGCGGCGCGCTGGATGGCAGCGTCGATGGCGGCGCTGATGACGACACCTTCATCGGCAATACC  >  NC_014034/2973721‑2973916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG