Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 297,560 A→G 100% K12E (AAG→GAG)  RCAP_rcc00260 → alanine racemase domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034297,5600A→G100.0% 815.4 217K12E (AAG→GAG) RCAP_rcc00260alanine racemase domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (131/86):  ref base (0/0):  total (131/86)

Only 100 of 229 total aligned reads displayed.

GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657
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gggCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAg                                                                                                 >  2:1849161/1‑101 (MQ=255)
 ggCCTTGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAgg                                                                                                >  1:3779491/1‑101 (MQ=255)
     ttGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAc                                                                                            <  1:1822181/101‑1 (MQ=255)
      tGGCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAcc                                                                                           >  2:1618879/1‑101 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGGTTCAGACCCGGATTGCGGAGGCGAccc                                                                                          >  1:635600/1‑101 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          >  1:1869140/1‑101 (MQ=255)
       ggCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGAccc                                                                                          <  2:909438/101‑1 (MQ=255)
        gCCCGC.TCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcg                                                                                         <  1:3393616/101‑1 (MQ=255)
            cgctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggc                                                                                      >  1:2624412/4‑101 (MQ=255)
              ctcttttctttTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCccggccg                                                                                    >  1:421870/2‑101 (MQ=255)
                cttttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcg                                                                                  >  2:3677916/1‑101 (MQ=255)
                 ttttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgg                                                                                 <  1:2957247/101‑1 (MQ=255)
                 ttttcttttTTGCCACCTTGTGCTCGATTGCGCCACAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgg                                                                                 >  1:4591828/1‑101 (MQ=255)
                  tttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcggg                                                                                >  1:1484907/1‑101 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               >  2:1571867/1‑101 (MQ=255)
                   ttcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggc                                                                               <  1:1849287/101‑1 (MQ=255)
                    tcttttTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggcc                                                                              <  1:624659/101‑1 (MQ=255)
                     cttttTTGCCGCCTTGTGCTCGATTGCGCCTCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCcgggccgcgggccg                                                                             <  1:4354922/101‑1 (MQ=255)
                      ttttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgg                                                                            >  1:1412769/1‑101 (MQ=255)
                      ttttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgg                                                                            >  2:2644020/1‑101 (MQ=255)
                      ttttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgg                                                                            >  2:1026280/1‑101 (MQ=255)
                        ttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgggc                                                                          >  2:464787/1‑101 (MQ=255)
                        ttttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGcgggccgggc                                                                          >  2:4017078/1‑101 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         <  1:4614224/101‑1 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         <  1:746498/101‑1 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         <  2:1450502/101‑1 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         >  1:1589909/1‑101 (MQ=255)
                         tttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCgggccgggcc                                                                         <  2:2449757/101‑1 (MQ=255)
                          ttGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGggccgggccg                                                                        >  1:1905538/1‑101 (MQ=255)
                           tGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGa                                                                       >  1:2077682/1‑101 (MQ=255)
                            gCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAg                                                                      >  2:4602021/1‑101 (MQ=255)
                                 cTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGc                                                                 >  1:4554600/1‑101 (MQ=255)
                                 cTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGc                                                                 <  1:1061113/101‑1 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  2:2321997/1‑101 (MQ=255)
                                   tgtgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCgg                                                               >  1:2912436/1‑101 (MQ=255)
                                     tgCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTg                                                             >  2:3653190/1‑101 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            <  2:3217583/101‑1 (MQ=255)
                                      gCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGc                                                            >  1:854405/1‑101 (MQ=255)
                                        tCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCgg                                                          >  1:3231374/1‑101 (MQ=255)
                                        tCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCgg                                                          >  1:3827195/1‑101 (MQ=255)
                                         cGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGc                                                         >  1:3877497/1‑101 (MQ=255)
                                         cGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGc                                                         >  1:2001529/1‑101 (MQ=255)
                                          gATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCt                                                        >  2:2943262/1‑101 (MQ=255)
                                          gATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCt                                                        >  1:1452896/1‑101 (MQ=255)
                                            ttGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGa                                                      <  1:3559712/101‑1 (MQ=255)
                                             tGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAt                                                     <  2:2588284/101‑1 (MQ=255)
                                             tGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGAt                                                     <  2:1728093/101‑1 (MQ=255)
                                               cgccgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCg                                                   >  1:3635608/1‑101 (MQ=255)
                                                  cgcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCg                                                >  2:2834907/1‑101 (MQ=255)
                                                   gcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgt                                               <  2:4070929/101‑1 (MQ=255)
                                                   gcAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCgg                                               >  2:1190722/1‑100 (MQ=255)
                                                    cAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCgtg                                              >  2:747255/1‑101 (MQ=255)
                                                      aaGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTc                                            >  1:3042592/1‑101 (MQ=255)
                                                      aaGGAGATCCCCATGGGATTGCAGGAGATTGAGCCCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTc                                            <  2:1916214/101‑1 (MQ=255)
                                                       aGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCg                                           <  2:3480135/101‑1 (MQ=255)
                                                         gagaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGaa                                         <  2:4477737/101‑1 (MQ=255)
                                                         gagaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGACGAGGGGGCGGTGCGGATGATCGCCGTGTCGaa                                         >  2:2037133/1‑101 (MQ=255)
                                                          agaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAg                                        >  2:1957678/1‑101 (MQ=255)
                                                          agaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAg                                        >  1:2412875/1‑101 (MQ=255)
                                                           gaTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCGTGTCGAAgg                                       >  1:2842968/1‑101 (MQ=255)
                                                            aTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGt                                      <  2:3083659/101‑1 (MQ=255)
                                                            aTCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCGTGTCGAAggg                                      >  1:1178036/1‑100 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGCCCCGGATGGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  1:921677/101‑1 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGc                                    <  1:1752837/101‑1 (MQ=255)
                                                              cGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGGGCGGCTGATCGCCGTGGCGAAGGTGc                                    >  1:4333377/1‑101 (MQ=255)
                                                               gCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCa                                   >  2:320981/1‑101 (MQ=255)
                                                               gCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCa                                   <  1:2145848/101‑1 (MQ=255)
                                                                gcATGGGATTGCAGCAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAg                                  <  2:3114802/100‑1 (MQ=255)
                                                                ccATGGGATTGCAGGAGATGCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAg                                  <  1:3210086/101‑1 (MQ=255)
                                                                 cATGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgc                                 <  1:949113/101‑1 (MQ=255)
                                                                  aTGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgcc                                >  1:163523/1‑101 (MQ=255)
                                                                  aTGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgcc                                <  2:3826910/101‑1 (MQ=255)
                                                                  aTGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGGGCAgcc                                >  1:4445939/1‑101 (MQ=255)
                                                                   tGGGATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccg                               >  1:223736/1‑101 (MQ=255)
                                                                     ggATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAgccgcc                             <  2:2720122/101‑1 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGAGGTGACCCGGGCCGCGGGCCGGGCCGCGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            <  1:3991363/101‑1 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            >  2:2991195/1‑101 (MQ=255)
                                                                      gATTGCAGGAGATTCAGACCCGGATGGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGccc                            <  1:3666036/101‑1 (MQ=255)
                                                                       gttGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCg                           <  1:4155855/100‑1 (MQ=255)
                                                                        ttGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          <  2:4543616/101‑1 (MQ=255)
                                                                        ttGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGa                          >  2:925658/1‑101 (MQ=255)
                                                                         tGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAg                         >  1:2591621/1‑101 (MQ=255)
                                                                         tGCAGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAg                         >  1:2468637/1‑101 (MQ=255)
                                                                         tGCAGGAGATTCAGACCCGGATGGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAg                         <  2:3611418/101‑1 (MQ=255)
                                                                            aGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCgg                      >  2:1789503/1‑101 (MQ=255)
                                                                            aGGAGATTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGGGCAGCCGCCCGAGCgg                      >  1:4369898/1‑101 (MQ=255)
                                                                              gagaTTCAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGt                    <  2:1357196/101‑1 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              >  2:986510/1‑101 (MQ=255)
                                                                                    cAGACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGc              >  1:1259100/1‑101 (MQ=255)
                                                                                      gACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCgg            >  1:632553/1‑101 (MQ=255)
                                                                                      gACCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCgg            >  2:785831/1‑101 (MQ=255)
                                                                                       gccgggATGGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGt           <  2:1895275/97‑1 (MQ=255)
                                                                                       aCCCGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCggg           >  2:689532/1‑100 (MQ=255)
                                                                                         ccGGATTGCGGGGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGc         <  1:425559/101‑1 (MQ=255)
                                                                                         ccGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGc         >  2:2551244/1‑101 (MQ=255)
                                                                                         ccGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGc         <  1:1736497/101‑1 (MQ=255)
                                                                                          cGGATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCt        >  2:4367400/1‑101 (MQ=255)
                                                                                           ggATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTc       <  1:3425573/101‑1 (MQ=255)
                                                                                           ggATTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTc       <  1:3122510/101‑1 (MQ=255)
                                                                                             aTTGCGGAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGGGCTGGCGGTGCTCgc     >  1:1651998/1‑100 (MQ=255)
                                                                                                   |                                                                                                 
GGGCCTTGGCCCGCTTCTTTTCTTTTTTGCCACCTTGTGCTCGATTGCGCCGCAAAGGAGATCGCCATGGGATTGCAGGAGATTCAGACCCGGATTGCGAAGGCGACCCGGGCCGCGGGCCGGGCCGAGGGGGCGGTGCGGCTGATCGCCGTGTCGAAGGTGCAGCCGCCCGAGCGGGTGCTGGCGGTGCTCGATCA  >  NC_014034/297461‑297657

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG