Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,037 T→G 11.2% intergenic (+83/+93) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0370T→G11.2% 60.3 ‑2.0 ‑0.2227intergenic (+83/+93)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (9/16):  ref base (106/93):  total (117/110)
Fisher's exact test strand distribution p-value = 1.37e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.72e-01

Only 100 of 254 total aligned reads displayed.

CGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTG  >  NC_014034/3003937‑3004138
                                                                                                     |                                                                                                      
cgcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.At                                                                                                        >  2:2629979/1‑101 (MQ=255)
cgcgcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.At                                                                                                        <  2:36007/101‑1 (MQ=255)
   gcgGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATccc                                                                                                     <  2:4348480/101‑1 (MQ=255)
   gcgGGCGAGAGCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATccc                                                                                                     <  2:555673/101‑1 (MQ=255)
    cgGTCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCg                                                                                                    <  2:3674490/101‑1 (MQ=255)
     gggCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCgg                                                                                                   >  1:247209/1‑101 (MQ=255)
        cGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGttt                                                                                                >  1:3113146/1‑101 (MQ=255)
         gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                               >  2:1477882/1‑101 (MQ=255)
         gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                               >  1:2090460/1‑101 (MQ=255)
         gagaTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTg                                                                                               <  2:4127653/101‑1 (MQ=255)
            aTCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCt                                                                                            >  2:916379/1‑101 (MQ=255)
               tGAGGGCAAGGCGGGGGGCGCTG.CCCCCTCTTGGCCTGACGGCCAATTTAACCCCCCTGGTATTTGGTGATAGGTGAAGGCAG.AACCCGGGTTGGCt.tttg                                                                                       >  2:613638/1‑99 (MQ=16)
                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCtt                                                                                   <  2:3733064/101‑1 (MQ=255)
                    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCtt                                                                                   <  2:214119/101‑1 (MQ=255)
                     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGCGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTg                                                                                  >  1:2620576/1‑101 (MQ=255)
                       aGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGgc                                                                                >  2:4608432/1‑101 (MQ=255)
                         gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGgcgc                                                                              <  1:4270976/101‑1 (MQ=255)
                         gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGgcgc                                                                              >  1:3485191/1‑101 (MQ=255)
                         gCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGgcgc                                                                              >  1:1187307/1‑101 (MQ=255)
                          cGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCa                                                                             >  2:3476269/1‑101 (MQ=255)
                             ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAat                                                                          <  2:1829186/101‑1 (MQ=255)
                              gggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAata                                                                         <  2:3756033/101‑1 (MQ=255)
                               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAatat                                                                        >  1:730499/1‑101 (MQ=255)
                               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAatat                                                                        <  1:4385071/101‑1 (MQ=255)
                               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAatat                                                                        <  1:902216/101‑1 (MQ=255)
                                gcgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATc                                                                       <  1:1458694/101‑1 (MQ=255)
                                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATcc                                                                      <  1:3414656/101‑1 (MQ=255)
                                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATcc                                                                      <  1:4496403/101‑1 (MQ=255)
                                  gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATccc                                                                     <  1:4660428/101‑1 (MQ=255)
                                    tGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCgg                                                                   <  1:3293621/101‑1 (MQ=255)
                                     gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGGTATTTTGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCggg                                                                  >  2:3209028/1‑101 (MQ=255)
                                      ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCgggg                                                                 >  1:4332976/1‑101 (MQ=255)
                                      ccccccTCTTGGCCT.TCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGAT.AAAGAAG.GTC....TTTTCCT.TTCATCTTGCCCGAAATATCCCgggggagccc                                                           <  2:2802396/101‑6 (MQ=37)
                                       cccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCggggg                                                                >  1:2270820/1‑101 (MQ=255)
                                         cccTTTTGTCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGa                                                              <  2:2849876/101‑1 (MQ=255)
                                           ctctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGt                                                            >  2:845171/1‑101 (MQ=255)
                                             ctTGGCCTGACGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                          <  1:4582920/101‑1 (MQ=255)
                                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                       <  1:3904808/101‑1 (MQ=255)
                                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                       <  2:3142076/101‑1 (MQ=255)
                                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                       <  2:3626324/101‑1 (MQ=255)
                                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGCCCcgc                                                       >  2:2192770/1‑101 (MQ=255)
                                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAACAG.ATCCCGGATTGCCT.TTCACCTTGGCGCAAATATCCAGGGGGGAGTGCcgc                                                       >  2:203028/1‑101 (MQ=25)
                                                ggCCTGACGGCCAATTCACCCCCCGGGACAATTGGGCCAAGGGGGAAACAG.ACCCCCCGTTGCCTCTTCA.CTCTGCGCCAATATCCCCGGGGGacccaccc                                                       >  1:3924822/1‑94 (MQ=255)
                                                 gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                      >  2:670840/1‑101 (MQ=255)
                                                  ccTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                                     <  1:3716250/101‑1 (MQ=255)
                                                  ccTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                                     >  1:11104/1‑101 (MQ=255)
                                                   cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcaga                                                    >  1:3666492/1‑98 (MQ=255)
                                                    tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                   >  1:2887370/1‑101 (MQ=255)
                                                     gACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggggc                                                  >  1:135650/1‑100 (MQ=255)
                                                       cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                <  2:13522/101‑1 (MQ=255)
                                                            caTTCCCCCCCCGGGATATTTGGACCAAGGTGAGAGCAG.ATCCCGGTTTGCCT.TTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                           <  1:4011745/100‑1 (MQ=255)
                                                            aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                           <  2:2690588/101‑1 (MQ=255)
                                                             agtCACCCCCCGGGATATTTGGACCAAGGTGAAGGCAG.ATCGCGGTTTGGCT.TTCACCTTGGCTTAAATCTCCCTGGGGGAGTCCCGCGGGGACGGGGGGc                                          <  2:1269875/99‑1 (MQ=25)
                                                             aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                          >  2:2512300/1‑101 (MQ=255)
                                                              ttCTCCCGCCGGGATATTTGGCCGAGGGTGAAAGCAG.ACCCGGGTTTGCCT.TTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                         <  1:2064993/101‑1 (MQ=21)
                                                               tccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                        <  1:2015420/98‑1 (MQ=255)
                                                               tCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                        >  1:3525995/1‑101 (MQ=255)
                                                                ccccccccGGGCTATTTGGCCCAAGGTGAAAGCCG.CGCCCGGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                       <  2:1059687/99‑2 (MQ=17)
                                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                       >  2:2106360/3‑100 (MQ=18)
                                                                ccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                       <  1:2575585/99‑1 (MQ=255)
                                                                 cccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                      <  2:82242/100‑1 (MQ=255)
                                                                 aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                      >  1:2989099/1‑101 (MQ=255)
                                                                 aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                      >  2:1408471/1‑101 (MQ=255)
                                                                   cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                    >  2:4339955/1‑101 (MQ=255)
                                                                   cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                    <  1:1492487/101‑1 (MQ=255)
                                                                    ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                   >  2:1100813/1‑101 (MQ=255)
                                                                      ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                 >  1:1272337/1‑101 (MQ=255)
                                                                        gggATTTTGGGACCAAGGGGAAAGCAG.ATCCGGTTTTTCCT.TCCACCTTGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                               <  1:3243706/101‑1 (MQ=21)
                                                                        gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                               <  1:1788877/101‑1 (MQ=255)
                                                                         ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                              <  1:1516161/101‑1 (MQ=255)
                                                                         ggATATTTGGACCAAGGTGAAAGCAG.ACCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                              <  1:1033241/101‑1 (MQ=255)
                                                                          gATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccccc                             >  2:2358331/1‑98 (MQ=18)
                                                                           atatTTGGCCCAAGGTGAAAGCCG.CGCCCGGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                            <  2:1750264/101‑5 (MQ=14)
                                                                           atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                            <  2:3276209/101‑5 (MQ=17)
                                                                           atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                            <  2:2270658/101‑1 (MQ=255)
                                                                             atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                          >  1:409687/1‑95 (MQ=18)
                                                                             atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                          >  2:2419576/1‑95 (MQ=18)
                                                                             atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCAT.TTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                          <  1:4952/101‑7 (MQ=17)
                                                                             atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                          >  1:2005415/1‑101 (MQ=255)
                                                                                tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGGGGAgggccccccccccccc                       >  1:165051/1‑100 (MQ=37)
                                                                                tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                       <  1:1053531/101‑1 (MQ=255)
                                                                                tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                       >  2:4369295/1‑101 (MQ=255)
                                                                                  gACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgg                     <  2:1841219/101‑1 (MQ=255)
                                                                                   aCCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                    <  2:2887154/101‑1 (MQ=255)
                                                                                    ccAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                   >  1:1085782/1‑101 (MQ=255)
                                                                                    ccAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                   >  2:1502867/1‑101 (MQ=255)
                                                                                     cAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                  >  2:1569270/1‑101 (MQ=255)
                                                                                      aaGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                 >  1:557947/1‑101 (MQ=255)
                                                                                       aGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                >  2:2744549/1‑101 (MQ=255)
                                                                                         gTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg              >  2:3412294/1‑101 (MQ=255)
                                                                                          tGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc             >  1:1724976/1‑101 (MQ=255)
                                                                                           gAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg            >  2:1740441/1‑101 (MQ=255)
                                                                                           gAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg            >  2:3005348/1‑101 (MQ=255)
                                                                                            aaaGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc           <  2:2710802/101‑1 (MQ=255)
                                                                                             aaGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt          >  2:4235646/1‑101 (MQ=255)
                                                                                              aGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTc         >  1:647880/1‑101 (MQ=255)
                                                                                                cAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg       >  2:4377389/1‑101 (MQ=255)
                                                                                                  g.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGACCCCCTGCCCACCTGGGGCCGAGCGCTCAgga     >  2:4049050/1‑100 (MQ=255)
                                                                                                     tCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGGGCCCCGGGGGGCGGGGGGGCAGAGCCCCCCT.CCCCCCCGGGGCCCGGCGCCCCGGCggg  >  1:274194/1‑99 (MQ=21)
                                                                                                     tCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt   >  2:1644985/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
CGCGCGGGCGAGATCTGAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCT.TTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTG  >  NC_014034/3003937‑3004138

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG