Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,053 A→C 13.9% intergenic (+99/+77) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0530A→C13.9% 74.7 ‑2.9 ‑0.5223intergenic (+99/+77)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (12/20):  ref base (110/81):  total (122/101)
Fisher's exact test strand distribution p-value = 5.37e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.24e-01

Only 100 of 252 total aligned reads displayed.

GAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTC  >  NC_014034/3003953‑3004153
                                                                                                      |                                                                                                    
    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGGCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCtt                                                                                                  <  1:4421820/101‑1 (MQ=255)
    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGT.AG.ATCCCGGTTTGCCTTTCACCtt                                                                                                  <  2:1306654/101‑1 (MQ=255)
    gCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCtt                                                                                                  <  2:3733064/101‑1 (MQ=255)
     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTg                                                                                                 >  2:729128/1‑101 (MQ=255)
     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTg                                                                                                 <  1:1437651/101‑1 (MQ=255)
     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGCGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTg                                                                                                 >  1:2620576/1‑101 (MQ=255)
     cAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCCGGGCATTTTGACCAAGGGGAAAACAAA.ATCCCGGTTTGCTTTTACCCtt                                                                                                  >  1:2473974/1‑101 (MQ=18)
      aaGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTgg                                                                                                >  2:4342110/1‑101 (MQ=255)
        ggCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGgcg                                                                                              >  2:1454304/1‑101 (MQ=255)
            gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                          <  2:13621/101‑1 (MQ=255)
            gggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCaaa                                                                                          <  2:2419487/101‑1 (MQ=255)
             ggggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAat                                                                                         <  2:1829186/101‑1 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                       <  1:4385071/101‑1 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                       >  1:730499/1‑101 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                       <  1:902216/101‑1 (MQ=255)
               ggCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAatat                                                                                       <  1:1162191/101‑1 (MQ=255)
                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                     <  1:4586535/101‑1 (MQ=255)
                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                     <  1:3414656/101‑1 (MQ=255)
                 cgcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATcc                                                                                     <  1:3270771/101‑1 (MQ=255)
                  gcTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATccc                                                                                    <  1:4660428/101‑1 (MQ=255)
                   cTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGGTATTTTGACCAAGGTGAAAGC.AG.ATACCCGGTTGCCTTTCCCCCTGGGGCCAATATCCCg                                                                                   >  1:3450069/1‑101 (MQ=21)
                    tGCCCCCCTCTTGGACTGACGGCCAATTCACCCCCCGGGATATTTTGTCCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTTCACTTGGCGCAAATATCCCgg                                                                                  >  2:2135096/1‑101 (MQ=21)
                     gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGGTATTTTGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                                 >  2:3209028/1‑101 (MQ=255)
                     gCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCggg                                                                                 <  2:2278608/101‑1 (MQ=255)
                      ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                                                <  1:130129/101‑1 (MQ=255)
                      ccccccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCgggg                                                                                >  1:4332976/1‑101 (MQ=255)
                         cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                             >  2:3559993/1‑101 (MQ=255)
                         cccTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGa                                                                             <  1:1477991/101‑1 (MQ=255)
                           ctctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGt                                                                           >  2:4473011/1‑101 (MQ=255)
                             ctTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTcc                                                                         >  2:590629/1‑101 (MQ=255)
                              ttGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTccc                                                                        <  1:4543776/101‑1 (MQ=255)
                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                      <  2:3626324/101‑1 (MQ=255)
                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                      <  1:3904808/101‑1 (MQ=255)
                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgc                                                                      <  2:3142076/101‑1 (MQ=255)
                                ggCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGCCCcgc                                                                      >  2:2192770/1‑101 (MQ=255)
                                 gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                                     >  2:3684381/1‑101 (MQ=255)
                                    tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                  >  1:2887370/1‑101 (MQ=255)
                                    tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                  >  2:513203/1‑101 (MQ=255)
                                      aCGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                <  1:681154/101‑1 (MQ=255)
                                          ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                            >  1:2353347/1‑101 (MQ=255)
                                           cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                           <  1:1752877/101‑1 (MQ=255)
                                            caTTCCCCCCCCGGGATATTTGGACCAAGGTGAGAGC.AG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                          <  1:4011745/100‑1 (MQ=255)
                                             aTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGc                                                         >  2:2512300/1‑101 (MQ=255)
                                              ttCTCCCGCCGGGATATTTGGCCGAGGGTGAAAGC.AG.ACCCGGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCa                                                        <  1:2064993/101‑1 (MQ=21)
                                              ttCACCCCCCGGGAGATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGTCGAGTCCCGCGGGGCCggcgggac                                                        >  2:3009873/1‑99 (MQ=25)
                                               tccccccccGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                       <  1:2015420/98‑1 (MQ=255)
                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                      <  2:4238676/99‑2 (MQ=18)
                                                ccccccccGGGATATTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                      >  2:2106360/3‑100 (MQ=18)
                                                ccccccccGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                      <  1:2575585/99‑1 (MQ=255)
                                                 cccccccGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                     <  2:82242/100‑1 (MQ=255)
                                                    ccccGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                                  >  2:1100813/1‑101 (MQ=255)
                                                      ccGGGATATTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                                <  2:4356980/101‑1 (MQ=17)
                                                      ccGGGATATTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGACGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                                <  2:1542610/101‑1 (MQ=17)
                                                      ccGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                >  1:1272337/1‑101 (MQ=255)
                                                       cGGGATATTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                               >  1:3508824/1‑101 (MQ=17)
                                                        gggATTTTGGGACCAAGGGGAAAGC.AG.ATCCGGTTTTTCCTTCCACCTTGGCGAAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                              <  1:3243706/101‑1 (MQ=21)
                                                        gggATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                              <  1:1788877/101‑1 (MQ=255)
                                                        gggATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                              >  1:4175319/1‑101 (MQ=255)
                                                        gggATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                              <  2:1482353/101‑1 (MQ=255)
                                                        gggATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                              >  2:3226451/1‑101 (MQ=255)
                                                         ggATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                             >  1:3993173/1‑101 (MQ=255)
                                                         ggATATTTGGACCAAGGTGAAAGC.AG.ACCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                             <  1:1033241/101‑1 (MQ=255)
                                                          gATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                            >  2:3773043/1‑101 (MQ=255)
                                                           atatTTGGCCCAAGGTGAAAGC.CGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgacccccctct                                           >  2:894607/1‑97 (MQ=18)
                                                           atatTTGGCCCAAGGTGAAAGC.CG.CGCCCGGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                           <  2:1750264/101‑5 (MQ=14)
                                                             atTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                         <  1:1493754/101‑7 (MQ=17)
                                                             atTTGGCCCAAGGTGAAAGC.CG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                         <  1:4952/101‑7 (MQ=17)
                                                             atTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                         >  1:2005415/1‑101 (MQ=255)
                                                             atTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                         <  1:3622581/101‑1 (MQ=255)
                                                               ttGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCCTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccc                                       >  2:1219185/1‑101 (MQ=255)
                                                                tGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGGGGAgggccccccccccccc                                      >  1:165051/1‑100 (MQ=37)
                                                                tGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                      <  1:1053531/101‑1 (MQ=255)
                                                                tGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                      <  2:3449815/101‑1 (MQ=255)
                                                                 ggcccAAGTTGAAAAC.AG.ATCCGGGTTCGCGTTTCTCCTTGACGCAAGTATCCCGGGGGGAGTCCGGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                     <  1:3010102/98‑1 (MQ=21)
                                                                 ggACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                     >  2:1039636/1‑101 (MQ=255)
                                                                   aacAAGTTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                   <  2:2860062/99‑1 (MQ=255)
                                                                     cAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                 <  1:1208833/101‑1 (MQ=255)
                                                                      aaGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGcc                                >  1:557947/1‑101 (MQ=255)
                                                                       aGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCg                               >  2:2744549/1‑101 (MQ=255)
                                                                         gTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                             <  1:3684656/101‑1 (MQ=255)
                                                                          tGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                            <  1:1100902/101‑1 (MQ=255)
                                                                          tGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                            >  2:1964865/1‑101 (MQ=255)
                                                                            aaaGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                          <  2:2710802/101‑1 (MQ=255)
                                                                               gC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCa                       <  1:2135246/101‑1 (MQ=255)
                                                                                c.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                      >  2:4377389/1‑101 (MQ=255)
                                                                                   g.ATCCCGGTTTGCCTTTCGCCTTGGCGCAAATATCCCGGGGGAAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                    <  2:2647562/101‑1 (MQ=255)
                                                                                   g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGACCCCCTGCCCACCTGGGGCCGAGCGCTCAgga                    >  2:4049050/1‑100 (MQ=255)
                                                                                      tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGGGCCCCGGGGGGCGGGGGGGCAGAGCCCCCCT.CCCCCCCGGGGCCCGGCGCCCCGGCggg                 >  1:274194/1‑99 (MQ=21)
                                                                                      tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                  >  2:1644985/1‑101 (MQ=255)
                                                                                        ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt                >  2:4232061/1‑101 (MQ=255)
                                                                                           gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg             >  1:818311/1‑101 (MQ=255)
                                                                                              tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg          >  2:1515073/1‑101 (MQ=255)
                                                                                               gCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGa         <  2:2888236/101‑1 (MQ=255)
                                                                                                ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg        >  1:1707994/1‑101 (MQ=255)
                                                                                                 cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc       >  2:231301/1‑101 (MQ=255)
                                                                                                  tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc      <  2:1852797/101‑1 (MQ=255)
                                                                                                  tttCACCTGGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc      <  1:590690/101‑1 (MQ=255)
                                                                                                      aCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctc  >  2:3410820/1‑101 (MQ=255)
                                                                                                      aCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctc  >  2:2835653/1‑101 (MQ=255)
                                                                                                      aCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctc  >  2:173754/1‑101 (MQ=255)
                                                                                                      |                                                                                                    
GAGGGCAAGGCGGGGGGCGCTGCCCCCCTCTTGGCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGC.AG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTC  >  NC_014034/3003953‑3004153

Base quality scores:  ATCG  < 3 ≤  ATCG  < 16 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG