Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,004,086 G→A 13.2% intergenic (+132/+44) gfo → / ← lrp glucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,004,0860G→A13.2% 69.9 ‑3.8 ‑0.2204intergenic (+132/+44)gfo/lrpglucose‑‑fructose oxidoreductase/leucine‑responsive regulatory protein
Reads supporting (aligned to +/- strand):  new base (10/17):  ref base (107/69):  total (117/87)
Fisher's exact test strand distribution p-value = 2.27e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.93e-01

Only 100 of 237 total aligned reads displayed.

GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTC  >  NC_014034/3003986‑3004186
                                                                                                     |                                                                                                    
gCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCcgcg                                                                                                      >  2:670840/1‑101 (MQ=255)
 ccTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgg                                                                                                     <  1:3716250/101‑1 (MQ=255)
  cTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCggg                                                                                                    <  1:2542135/101‑1 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   >  1:2887370/1‑101 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   >  2:513203/1‑101 (MQ=255)
   tGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCgggg                                                                                                   <  2:823385/101‑1 (MQ=255)
     aCGGCCAATTCCCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAc                                                                                                 <  1:681154/101‑1 (MQ=255)
      cGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACg                                                                                                <  2:13522/101‑1 (MQ=255)
        gCCAATTCACCCCCGGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTGGGCGCAAATATCCCGGGGGGAGTCCCGCTGGGACggg                                                                                              <  2:1739157/101‑1 (MQ=255)
         ccAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggg                                                                                             >  1:2353347/1‑101 (MQ=255)
          cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                                                            <  1:571490/101‑1 (MQ=255)
          cAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACggggg                                                                                            <  1:1752877/101‑1 (MQ=255)
           caTTCCCCCCCCGGGATATTTGGACCAAGGTGAGAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           <  1:4011745/100‑1 (MQ=255)
           aaTTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACgggggg                                                                                           <  2:2690588/101‑1 (MQ=255)
            agtCACCCCCCGGGATATTTGGACCAAGGTGAAGGCAG.ATCGCGGTTTGGCTTTCACCTTGGCTTAAATCTCCCTGGGGGAGTCCCGCGGGGACGGGGGGc                                                                                          <  2:1269875/99‑1 (MQ=25)
              tccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCag                                                                                        <  1:2015420/98‑1 (MQ=255)
               ccccccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCagc                                                                                       <  1:2632666/99‑2 (MQ=18)
               ccccccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCaga                                                                                       <  1:2575585/99‑1 (MQ=255)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                      >  2:1408471/1‑101 (MQ=255)
                aCCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCACGGGGGGAGTCCCGCGGGGACGGGGGGCagag                                                                                      >  1:1920769/1‑101 (MQ=255)
                  cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGGAGCgcc                                                                                    >  1:3257655/1‑101 (MQ=18)
                  cccccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcc                                                                                    >  1:3627352/1‑101 (MQ=18)
                  cccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcc                                                                                    <  1:1492487/101‑1 (MQ=255)
                   ccccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccc                                                                                   >  2:1100813/1‑101 (MQ=255)
                     ccGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGACGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccc                                                                                 <  2:1542610/101‑1 (MQ=17)
                     ccGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgccccc                                                                                 >  1:1272337/1‑101 (MQ=255)
                      cGGGATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgcccccc                                                                                >  1:3508824/1‑101 (MQ=17)
                       gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                                               <  1:1788877/101‑1 (MQ=255)
                       gggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccct                                                                               >  2:1836164/1‑101 (MQ=255)
                        ggATATTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccct                                                                              <  1:2069046/101‑3 (MQ=17)
                        ggATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctg                                                                              <  1:1516161/101‑1 (MQ=255)
                         gATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgc                                                                             >  2:3773043/1‑101 (MQ=255)
                          atatTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctct                                                                            <  2:3276209/101‑5 (MQ=17)
                          atatTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcc                                                                            <  2:2270658/101‑1 (MQ=255)
                            atTTGGCCCAAGGTGAAAGCCGCGCCCCGGGTT.CATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                                                          >  1:409687/1‑95 (MQ=18)
                            atTTGGCCCAAGGTGAAAGCCG.CGCCCCGGGTTCATTTCCCCTTGGCGGAAATATCCCGGGGGGAGTCCCGCAGGGACGGGGGGCAGCgccccccctcttc                                                                          <  2:4353141/101‑7 (MQ=17)
                            atTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccc                                                                          <  1:3622581/101‑1 (MQ=255)
                               tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                                                       <  2:4067821/101‑1 (MQ=255)
                               tGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAgcccccctgcccccct                                                                       <  1:1053531/101‑1 (MQ=255)
                                ggACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGcccccctgcccccctg                                                                      >  2:1039636/1‑101 (MQ=255)
                                  aacAAGTTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTggg                                                                    <  2:2860062/99‑1 (MQ=255)
                                   ccAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTgggg                                                                   >  2:1502867/1‑101 (MQ=255)
                                    cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                  >  2:2837204/1‑101 (MQ=255)
                                    cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                  >  2:3720568/1‑101 (MQ=255)
                                    cAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGc                                                                  <  1:1208833/101‑1 (MQ=255)
                                      ccgTGAAAGCAG.ATCCCGGTTTGCCTTTCCCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCCGCGCCCCCCTGCCCCCCTGCGGCCg                                                                >  2:669386/3‑101 (MQ=25)
                                       ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                                               >  2:4454298/1‑101 (MQ=255)
                                       ggTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGa                                                               <  2:1187215/101‑1 (MQ=255)
                                        gTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAg                                                              >  2:3412294/1‑101 (MQ=255)
                                         taaaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                             >  1:1724975/3‑101 (MQ=255)
                                         tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                             <  1:1100902/101‑1 (MQ=255)
                                         tGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgc                                                             >  1:3226809/1‑101 (MQ=255)
                                          gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                                                            >  1:1638207/1‑101 (MQ=255)
                                          gAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcg                                                            >  2:3005348/1‑101 (MQ=255)
                                           aaaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAgcgc                                                           <  2:2710802/101‑1 (MQ=255)
                                            aaGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCt                                                          >  2:4235646/1‑101 (MQ=255)
                                               cAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAg                                                       >  2:4377389/1‑101 (MQ=255)
                                                 g.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGc                                                     >  1:2061630/1‑101 (MQ=255)
                                                   aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg                                                    >  1:499712/1‑101 (MQ=255)
                                                   aTCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCg                                                    >  1:2987005/1‑101 (MQ=255)
                                                    tCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgt                                                   >  2:1644985/1‑101 (MQ=255)
                                                      ccGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCgtgt                                                 <  1:613699/101‑1 (MQ=255)
                                                         gTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTg                                              >  1:818311/1‑101 (MQ=255)
                                                            tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                           >  2:3855647/1‑101 (MQ=255)
                                                            tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                           >  2:101064/1‑101 (MQ=255)
                                                            tGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCg                                           >  2:1515073/1‑101 (MQ=255)
                                                              ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg                                         <  1:893520/101‑1 (MQ=255)
                                                              ccTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAg                                         >  1:1707994/1‑101 (MQ=255)
                                                               cTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGc                                        >  1:1281010/1‑101 (MQ=255)
                                                                tttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGcc                                       <  2:1852797/101‑1 (MQ=255)
                                                                 ttCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCg                                      >  2:2298813/1‑101 (MQ=255)
                                                                  tCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGc                                     >  1:4654459/1‑101 (MQ=255)
                                                                    aCCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctc                                   >  2:3163355/1‑101 (MQ=255)
                                                                     ccTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGctct                                  <  1:1031041/101‑1 (MQ=255)
                                                                         ggCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGGTTGGCGAGCCGCTCTTCGa                              >  1:799166/1‑101 (MQ=255)
                                                                           cgcAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGc                            >  2:1364031/1‑101 (MQ=255)
                                                                            gcAAATATCCGGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCa                           <  1:494175/101‑1 (MQ=255)
                                                                             cAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCAc                          <  1:3033670/101‑1 (MQ=255)
                                                                               aaTATCCGGGGGGGAGTCCCGCGGGGACGGGGTGCATAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGt                        <  2:2090320/101‑1 (MQ=255)
                                                                                atatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                       <  1:4473306/101‑1 (MQ=255)
                                                                                atatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                       >  2:3035074/1‑101 (MQ=255)
                                                                                agaccccGGGGGGAGTCCCGCGGGGAGGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTc                       <  2:4359567/97‑1 (MQ=255)
                                                                                 tatCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCg                      >  2:801890/1‑101 (MQ=255)
                                                                                    cccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAg                   >  1:4187365/1‑101 (MQ=255)
                                                                                     cgggggggAGTCCGGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAgg                  <  2:2988870/99‑1 (MQ=255)
                                                                                     ccGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAgg                  >  2:2504624/1‑101 (MQ=255)
                                                                                       ggggggGGCCCCGGGGGGGGGGGGGGCAGACCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                <  1:2803178/101‑1 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                >  2:1754703/1‑101 (MQ=255)
                                                                                       ggggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGc                >  2:3472728/1‑101 (MQ=255)
                                                                                        gggggAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCa               >  2:2802962/1‑101 (MQ=255)
                                                                                           ggAGTCCCGCGGGGCCGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGc            <  2:2130619/101‑1 (MQ=255)
                                                                                           ggAGCCCCGCGGGGCCGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCGGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGc            <  1:3476527/101‑1 (MQ=255)
                                                                                              gTCCGGCGGGGAGGGGGTGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgg         <  2:4190493/101‑1 (MQ=255)
                                                                                              gTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCgg         >  2:4539069/1‑101 (MQ=255)
                                                                                               tCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCggg        >  2:2372573/1‑101 (MQ=255)
                                                                                                  cgcgGGGAGGGGGGGCAGCCCCCCCCGGCCCCCCTGGGGCCGGGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtc     <  2:3305314/101‑1 (MQ=255)
                                                                                                   gcgGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcg    >  2:2521897/1‑101 (MQ=255)
                                                                                                     ggggCCGGGGGGGAGTCCCCCCCTGCCCCCCTGGGGCCGGGCGCTCGGGGGTGTTTGGGGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgtc  <  1:513251/101‑1 (MQ=255)
                                                                                                     ggggACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgtc  >  1:2704083/1‑101 (MQ=255)
                                                                                                     ggggACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGgtcgtc  >  2:2047499/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
GCCTGACGGCCAATTCACCCCCCGGGATATTTGGACCAAGGTGAAAGCAG.ATCCCGGTTTGCCTTTCACCTTGGCGCAAATATCCCGGGGGGAGTCCCGCGGGGACGGGGGGCAGAGCCCCCCTGCCCCCCTGGGGCCGAGCGCTCAGGCGTGTTTGGCGAGCCGCTCTTCGAGCACGTCGAAGGGCACGCCGGGGTCGTC  >  NC_014034/3003986‑3004186

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG