Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,011,286 T→A 82.7% intergenic (+85/+57) purK → / ← RCAP_rcc02822 phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,011,2860T→A82.7% 106.9 0.0 0.0185intergenic (+85/+57)purK/RCAP_rcc02822phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Reads supporting (aligned to +/- strand):  new base (97/56):  ref base (21/11):  total (118/67)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 194 total aligned reads displayed.

TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379
                                                                                             |                                                                                             
  ctctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggg                                                                                     <  1:2938456/101‑1 (MQ=255)
   tctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCCCCTTGGGAC.AAATATCCCggggg                                                                                    >  1:2094395/1‑101 (MQ=37)
    ctGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCgggggg                                                                                   >  1:3823551/1‑101 (MQ=255)
     tGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGga                                                                                  >  2:3362084/1‑101 (MQ=255)
     tGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGga                                                                                  <  1:144152/101‑1 (MQ=255)
      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgag                                                                                 >  1:273007/1‑101 (MQ=255)
      gACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgag                                                                                 >  2:1986904/1‑101 (MQ=255)
       gccGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgaga                                                                                <  1:2870060/100‑1 (MQ=255)
       aCCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGgaga                                                                                <  1:3550101/101‑1 (MQ=255)
       aCCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGgaga                                                                                >  1:1030327/1‑101 (MQ=255)
         cgcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACt                                                                              <  1:4416946/101‑1 (MQ=255)
          gcgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACtt                                                                             >  1:3029438/1‑101 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTg                                                                            >  1:112195/1‑101 (MQ=255)
           cgGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTg                                                                            >  1:4628687/1‑101 (MQ=255)
            ggCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGc                                                                           <  2:4628386/101‑1 (MQ=255)
             gCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGcc                                                                          >  2:619612/1‑101 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGc                                                                        <  2:111567/101‑1 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGc                                                                        >  1:1035448/1‑101 (MQ=255)
               aGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGc                                                                        >  2:2094174/1‑101 (MQ=255)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                      >  1:2090759/1‑101 (MQ=255)
                 cGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAg                                                                      >  1:839282/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGGCGCAgg                                                                     >  1:1467969/1‑101 (MQ=37)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAgg                                                                     >  1:543958/1‑101 (MQ=255)
                  gggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTGGCCGCAgg                                                                     >  1:3158746/1‑101 (MQ=38)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                    >  1:3709641/1‑101 (MQ=255)
                   ggggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGc                                                                    >  2:2767690/1‑101 (MQ=255)
                    gggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCa                                                                   <  2:3981260/101‑1 (MQ=255)
                    gggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCa                                                                   >  1:1255268/1‑101 (MQ=255)
                    gggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCa                                                                   <  1:509183/101‑1 (MQ=255)
                    gggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCa                                                                   >  2:799579/1‑101 (MQ=255)
                     ggCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCaa                                                                  <  1:142176/101‑1 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                               >  2:1614184/1‑101 (MQ=255)
                        tGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAagag                                                               >  2:3769124/1‑101 (MQ=255)
                         gTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgg                                                              >  2:1275584/1‑101 (MQ=255)
                           cTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggg                                                            >  2:3781215/1‑101 (MQ=255)
                            ttcccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGACACTTGCCGCAGGCAAGAggggg                                                           <  2:608123/99‑1 (MQ=38)
                            tGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAggggg                                                           <  1:1631507/101‑1 (MQ=255)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                          >  2:3515114/1‑101 (MQ=255)
                             gCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAgggggg                                                          <  1:234615/101‑1 (MQ=255)
                              cccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGc                                                         <  1:4351949/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                        <  2:1030244/101‑1 (MQ=255)
                               ccccGCACCCCCGAGGATATTACCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCa                                                        >  1:2648718/1‑101 (MQ=39)
                                cccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAg                                                       <  1:4055677/101‑1 (MQ=255)
                                 ccGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgc                                                      <  1:2592584/101‑1 (MQ=255)
                                  cGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcg                                                     >  2:3546896/1‑101 (MQ=255)
                                   gCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAgcgc                                                    >  1:3673016/1‑101 (MQ=255)
                                     aCCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGccc                                                  >  2:1455374/1‑101 (MQ=255)
                                     aCCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGccc                                                  >  2:4265878/1‑101 (MQ=255)
                                        cccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGcccccc                                               >  1:2210981/1‑101 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              <  2:2648533/101‑1 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              >  2:3231338/1‑101 (MQ=255)
                                         ccGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCt                                              <  1:2035521/101‑1 (MQ=255)
                                           gAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGc                                            <  1:274367/101‑1 (MQ=255)
                                            aGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGcc                                           <  1:1392358/101‑1 (MQ=255)
                                            aGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGcc                                           <  2:2024804/101‑1 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                         >  1:3333267/1‑101 (MQ=255)
                                              gATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCg                                         <  2:1172071/101‑1 (MQ=255)
                                               atatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCgg                                        >  1:1920740/1‑101 (MQ=255)
                                               atatTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCgg                                        <  1:4203165/101‑1 (MQ=255)
                                                tatTTCCGCCACGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGc                                       <  1:2629752/101‑1 (MQ=37)
                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCa                                    >  2:690699/1‑101 (MQ=255)
                                                   ttCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCa                                    <  1:2455951/101‑1 (MQ=255)
                                                    tCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                   >  1:3104771/1‑101 (MQ=255)
                                                    tCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCAc                                   >  1:720919/1‑101 (MQ=255)
                                                     ccGCCAAGGTGAAAACAACGCCCTTTCGCCTTGGGAC.AAATATCGCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACg                                  <  2:1402612/101‑1 (MQ=37)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                 >  2:950753/1‑101 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                 >  2:4438215/1‑101 (MQ=255)
                                                      cGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACgg                                 >  2:3926796/1‑101 (MQ=255)
                                                         cAAGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAg                              >  1:32511/1‑101 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                             >  1:4504803/1‑101 (MQ=255)
                                                          aaGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAgg                             <  1:3488925/101‑1 (MQ=255)
                                                           aGGTGAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAggg                            <  2:2939845/101‑1 (MQ=255)
                                                             gTGAAAACAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGcc                          >  1:596826/1‑101 (MQ=255)
                                                               gAAAACAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAg                        <  2:2174434/101‑1 (MQ=255)
                                                                aaaaCAACGCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGc                       >  2:4066194/1‑101 (MQ=255)
                                                                 aaaCAACGCCCTTTCCCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAggacatcgc                      <  2:1943811/101‑8 (MQ=25)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAgtc                      >  2:4151288/1‑99 (MQ=255)
                                                                 aaaCAACGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                      <  1:3958862/101‑1 (MQ=255)
                                                                 aaaCAACGCCCTTTCACCTGGGGAC.AAAGAGCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGcc                      <  1:1887936/101‑1 (MQ=37)
                                                                    caccccccTTCCCCCTTGGGAA.AAATACCCCGGGGGGAGACTTGCCGGAGCAAAGAGGGGGGCAGCCCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCt                   <  2:3672741/96‑1 (MQ=19)
                                                                     aacGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCAGCTg                  >  2:4384061/1‑101 (MQ=37)
                                                                      acGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCGCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                 >  2:1487839/1‑101 (MQ=37)
                                                                      acGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTga                 >  2:3136840/1‑100 (MQ=255)
                                                                      acGCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGc                 >  2:370014/1‑101 (MQ=255)
                                                                        gCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa               >  1:488427/1‑101 (MQ=255)
                                                                        gCCCTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa               >  1:2012155/1‑101 (MQ=255)
                                                                        gCCCTTTCACCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCaa               <  2:518312/101‑1 (MQ=255)
                                                                           cTTTCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGc            >  1:3582480/1‑101 (MQ=255)
                                                                           cTTTCACCTTGGGACAAATA.TCCCGGGGGGAAACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGc            >  1:3200907/1‑101 (MQ=37)
                                                                              tCACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc         >  1:2479717/1‑101 (MQ=255)
                                                                              tCACCTGGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCcgc         <  2:2917231/101‑1 (MQ=37)
                                                                               cACCTTGGGACAAATA.TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCCGCCGCTGCAAGGCcgcg        >  1:2127168/1‑101 (MQ=25)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc       >  1:2995624/1‑101 (MQ=255)
                                                                                aCCTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCgcgc       >  1:393626/1‑101 (MQ=255)
                                                                                  cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCCAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCAGCTGCAAGGCCGCGccc     >  2:729274/1‑101 (MQ=37)
                                                                                  cTTGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGccc     <  2:2719876/101‑1 (MQ=255)
                                                                                   ttGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGcccc    >  2:132207/1‑101 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   >  1:2417208/1‑101 (MQ=255)
                                                                                    tGGGAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCa   >  1:1178528/1‑101 (MQ=255)
                                                                                     gggAC.AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAg  >  2:1297229/1‑101 (MQ=255)
                                                                                             |                                                                                             
TTCTCTGACCGCGGCAGCGGGGGCTGTCTGCCCCCGCACCCCCGAGGATATTTCCGCCAAGGTGAAAACAACGCCCTTTCACCTTGGGAC.AATAGTCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG  >  NC_014034/3011194‑3011379

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG