Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,150 A→G 2.5% intergenic (‑133/+159) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1500A→G 2.5% 2.4 ‑1.2 ‑1.3161intergenic (‑133/+159)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (96/61):  total (100/61)
Fisher's exact test strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.09e-02

Only 100 of 193 total aligned reads displayed.

CCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCG  >  NC_014034/3068051‑3068250
                                                                                                   |                                                                                                       
ccAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCaaa                                                                                                        <  2:1388468/101‑1 (MQ=255)
 cAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAg                                                                                                       >  2:224516/1‑101 (MQ=255)
    ttttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGcc                                                                                                    >  1:1326625/1‑101 (MQ=255)
     tttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGccc                                                                                                   <  1:197449/101‑1 (MQ=255)
     tttGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGccc                                                                                                   <  1:242152/101‑1 (MQ=255)
      ttGCGGATTCGCGCAACCGAAACGATGGGCAGGGTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCt                                                                                                  >  2:2933844/1‑101 (MQ=255)
       tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                                                 <  1:1908129/101‑1 (MQ=255)
       tGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTc                                                                                                 <  2:1974780/101‑1 (MQ=255)
        gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                                                <  2:4093014/101‑1 (MQ=255)
        gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                                                <  2:3574447/101‑1 (MQ=255)
        gCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCg                                                                                                >  2:3159797/1‑101 (MQ=255)
         cGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCgg                                                                                               <  2:876995/101‑1 (MQ=255)
          ggATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGc                                                                                              <  2:2808821/101‑1 (MQ=255)
           gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcc                                                                                             <  2:4456727/101‑1 (MQ=255)
           gATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcc                                                                                             <  2:3248754/101‑1 (MQ=255)
             ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGcccc                                                                                           >  1:2285753/1‑99 (MQ=255)
             ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAg                                                                                           <  2:4252209/101‑1 (MQ=255)
              tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgc                                                                                          <  2:1320955/101‑1 (MQ=255)
              ctgcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgc                                                                                          <  1:2511930/99‑1 (MQ=255)
               cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgcg                                                                                         <  1:1033073/101‑1 (MQ=255)
                gcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAgcgc                                                                                        <  2:2216653/101‑1 (MQ=255)
                  gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                                                      <  2:2554295/101‑1 (MQ=255)
                  gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccc                                                                                      >  1:4606424/1‑101 (MQ=255)
                    aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccgc                                                                                    >  1:2090697/1‑101 (MQ=255)
                    aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccgc                                                                                    >  2:3264382/1‑101 (MQ=255)
                    aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgccccc                                                                                    >  1:3946962/1‑99 (MQ=255)
                     aCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGcgcccgcc                                                                                   >  1:2226047/1‑101 (MQ=255)
                      ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCgcccgccc                                                                                  >  1:1765817/1‑101 (MQ=255)
                       cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                                                 <  2:1957165/101‑1 (MQ=255)
                       cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgcccc                                                                                 <  2:2660353/101‑1 (MQ=255)
                        gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccc                                                                                >  1:347799/1‑100 (MQ=255)
                        gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccc                                                                                >  1:192724/1‑100 (MQ=255)
                         aaaCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGcccgccccgc                                                                               >  2:4439120/1‑101 (MQ=255)
                           aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCcgccccgccc                                                                             <  1:1017780/101‑1 (MQ=255)
                             gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                           <  2:3941050/101‑1 (MQ=255)
                             gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                           <  1:169616/101‑1 (MQ=255)
                             gATGGGCAGGCTGACCGACGCCCTCGCCGAGGTCATGCGGAACAGTGGCGTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCAt                                                                           <  2:517154/101‑1 (MQ=255)
                              aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCacg                                                                          >  1:3491017/1‑99 (MQ=255)
                                gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCcgccccgcccccggc                                                                        >  1:1615280/1‑96 (MQ=255)
                                      gccgACCGAGGCCATCGCCGAGGTCAGGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGC.GGgcg.c                                                                <  1:1877874/98‑1 (MQ=255)
                                      gCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGgcg.c                                                                <  2:1603472/101‑1 (MQ=255)
                                       cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.Ct                                                               <  1:1092964/101‑1 (MQ=255)
                                       cTGACCGAGGCCAGGGCGGAGGTCGTGCGCAACTGGGGCCTCATGCCCGCCGAGGGCCTAAAGGCCCTCGGGCAGGGCCCGCCGCGCCCATGGC.GGGCG.Ct                                                               <  2:2025190/101‑1 (MQ=9)
                                        tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.Ctt                                                              >  1:1538519/1‑101 (MQ=255)
                                          aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTtct                                                            <  2:2174552/101‑1 (MQ=255)
                                           ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTtctc                                                           <  1:712306/101‑1 (MQ=255)
                                            cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGGG.CCTCTcg                                                          >  2:987639/1‑101 (MQ=255)
                                                gCCCTCGCCGAGGTCAGGCGGAACAGTGGCCTCGTGCCCGCCGAGGTCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccg                                                      <  1:2059160/101‑1 (MQ=17)
                                                gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATCGC.GGGGG.CCTCTcccccc                                                      >  1:929017/1‑100 (MQ=18)
                                                 ccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccgc                                                     >  2:3014188/1‑101 (MQ=255)
                                                  cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccgcc                                                    >  2:94074/1‑101 (MQ=255)
                                                  cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccgcc                                                    <  2:77223/101‑1 (MQ=255)
                                                  cATCGCCGAGGGCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCTGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccgcc                                                    <  2:2385829/101‑1 (MQ=255)
                                                   aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCcccccg                                                   >  1:2680681/1‑101 (MQ=255)
                                                    tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCgg                                                  <  1:2161992/101‑1 (MQ=255)
                                                     tgCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAAGGCACTCGGCCTGCCCCCGCCCCGCCCTCCGCGGGGCC.CCTCCcgcccgcccc                                                  >  2:4010428/2‑99 (MQ=21)
                                                     cGCCGAGGTCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCggg                                                 <  2:2090557/101‑1 (MQ=255)
                                                        cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCCgggcg                                              >  1:4369711/1‑101 (MQ=255)
                                                        cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCCgggcg                                              >  2:3863204/1‑101 (MQ=255)
                                                           ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGgggcgggc                                           <  1:4444452/101‑1 (MQ=255)
                                                            gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGGG.CTTCTCGCCCCCCGgggcggggg                                          >  1:318053/1‑99 (MQ=255)
                                                             tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCCCCCCCCGGGGGGGgggc                                         >  1:3163333/1‑101 (MQ=18)
                                                              cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGGCCGCCGGGGCGGGCGc.c                                       >  1:4510652/1‑101 (MQ=255)
                                                              cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGc.c                                       >  1:1292018/1‑101 (MQ=255)
                                                               aTGCGGAACAGTGGCCTCGTGACCGCCCAGGGCCCAAAGGCCCTCGGCCAGCCCCCGCCCCGCCCATGGC.GGGCT.CTTCTCGCCCGCCGGGGCGGGGGC.Cg                                      >  2:3116385/1‑101 (MQ=17)
                                                                 gCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGc                                    <  2:3490759/101‑1 (MQ=255)
                                                                  cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGC.CGGcc                                   >  1:4270656/1‑101 (MQ=255)
                                                                    gAACAGTGGCCTCGTGACCGCGGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCctc                                 <  2:4510357/101‑1 (MQ=255)
                                                                     aaCCGTGCCCGCGGGCCCGCCGAGGGCCCAAAGGCCCTCGCCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCctct                                <  2:1288729/101‑1 (MQ=14)
                                                                     aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGC.CGGCccct                                >  1:2560287/1‑101 (MQ=255)
                                                                     aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCCCCGGGGCGGGGGG.CGGCctcc                                >  1:2899676/1‑100 (MQ=255)
                                                                      aCATTGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGC.GGGGGCCTTTTCGCCGCCCGCGGGGGGCCG.CCGCctcc                                >  1:37447/1‑100 (MQ=14)
                                                                      aCAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTctg                               <  2:1438813/101‑1 (MQ=255)
                                                                       cggTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCCCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTctgc                              <  1:3083056/99‑1 (MQ=255)
                                                                       cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGC.CGGcccccgc                              >  1:1121434/1‑96 (MQ=255)
                                                                       cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CCTCTCGCCCCCCCGGGCGGGGGC.Cgggccccgc                              >  1:3795759/1‑94 (MQ=17)
                                                                        attGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGC.GGGCGCCTTCGCCCCGCCCGGGGGGGGGGC.Cgggcccccc                              >  1:3502596/3‑94 (MQ=14)
                                                                          tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGG.CGGCCCCcgctgc                           >  2:1168812/1‑101 (MQ=255)
                                                                          tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCtgctgc                           >  2:2283842/1‑101 (MQ=255)
                                                                          tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCCGGGCGGGGGC.CCGGCCCtcctgc                           >  2:1889618/1‑101 (MQ=14)
                                                                           ggCCCCGTGACCGCCGAGGGCCCAGAGGCCCTCGGCCTGCGCCCGCCCCGCCCATGGC.GGGCGCCTTCGCGGCGCCCGGGGGGGGCGCGCGGccccccccc                            >  1:3905482/1‑94 (MQ=11)
                                                                           ggCCCCGTGACCGCAGAGGGCCAAAAGCCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTgcg                          <  1:4658254/101‑1 (MQ=14)
                                                                                cGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCCGGGCGGGGGC.CGGCCTCTGCTGCGCCCgg                     >  2:1065322/1‑101 (MQ=255)
                                                                                 gTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGC.CGGCCCCCGCTGCGCCTggg                    >  2:3745336/1‑101 (MQ=255)
                                                                                 gTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CCGCCCCTGCTTCGCCTggg                    >  2:4647258/1‑101 (MQ=255)
                                                                                  tGACCGCCGGGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCCCCGGGGGAGGCGC.CGGGCTCCGCTGGGccctatc                   >  1:139513/1‑96 (MQ=11)
                                                                                  tGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGC.CCGGCCCCGCTGCGCCTgggg                   >  2:2495746/1‑101 (MQ=14)
                                                                                    ccggccgGGGCCCCAAGGGCCCCGGGCCCGCCCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGaa                 <  1:3014417/98‑1 (MQ=14)
                                                                                    cccgccgAGGGCCCAAAGGCCCTCGGCCTGCGCCCGCCCCGCCATCGGCGGGGGG.CGTCGGGCCCCCCGGGGGGGGGGG.GCGCGCCTGGTGCGCGTggggg                  >  1:2266289/2‑100 (MQ=2)
                                                                                      cgccgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGGAAc               >  1:3194271/1‑101 (MQ=255)
                                                                                       gccgAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGGGGGCGC.CGGCCTCTGCCGCGCCCGGGGGAACg              >  2:2921410/1‑101 (MQ=14)
                                                                                          gAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGGGC.CGGCCTCTGCTGCGCCCGGGGGAAAgggc           >  2:2847325/1‑99 (MQ=14)
                                                                                             gccccAAGGCCCCCCGCCCGGCCCCCGCCCCGCCCAGGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACTGTCTTg        <  2:1765698/99‑1 (MQ=14)
                                                                                               cccAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGAGC.CGGCCTCCGCTGCGCCCGGGGAAACggggtgggc      >  1:1162120/1‑94 (MQ=14)
                                                                                                ccAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCa     >  2:653584/1‑101 (MQ=255)
                                                                                                 cAAGGGCCCGCGGCAAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAc    <  1:1453898/101‑1 (MQ=255)
                                                                                                 cAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGGGGGCGC.CGGCCCCCGCCGCGCCTGGGGGAACGGGCTTGGccc    >  1:3564040/1‑99 (MQ=17)
                                                                                                 cAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCCGGGGGAACGGGCtttggcc    >  2:1721011/1‑96 (MQ=14)
                                                                                                  aaaGCCCCTCGCCCAGCCCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAcc   <  2:109345/101‑1 (MQ=255)
                                                                                                   aaGGCCCTCGGCCAGCGCCCGCCCCGCCCATTGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCCCCTGCGCCTCGGGAAACCGGCTTgggcccg  >  2:798112/1‑96 (MQ=14)
                                                                                                   |                                                                                                       
CCAATTTTGCGGATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTCGGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCCGGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCG  >  NC_014034/3068051‑3068250

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG