Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 310,392 T→G 8.0% T378P (ACC→CCC)  RCAP_rcc00272 ← heparinase II/III family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034310,3920T→G 8.0% 12.2 ‑3.4 0.0195T378P (ACC→CCC) RCAP_rcc00272heparinase II/III family protein
Reads supporting (aligned to +/- strand):  new base (17/4):  ref base (98/76):  total (115/80)
Fisher's exact test strand distribution p-value = 3.49e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

GTTTCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGCGTC  >  NC_014034/310292‑310492
                                                                                                    |                                                                                                    
ttttCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGt                                                                                                      <  1:430040/100‑1 (MQ=255)
  ttCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTgg                                                                                                    <  1:4595427/101‑1 (MQ=255)
  ttCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAAACCTCGATCCCCAGCGTCGAATGGCTGGGGGGgg                                                                                                    >  1:1476800/1‑101 (MQ=255)
   tCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGGATGGCTGGGGGGggg                                                                                                   >  2:3550190/1‑100 (MQ=255)
    ccTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGgcg                                                                                                  <  1:2858053/101‑1 (MQ=255)
      tCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGggcg                                                                                                >  2:2549140/1‑101 (MQ=255)
       cGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGGGGGGGCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGggggggg                                                                                               >  1:3299837/1‑96 (MQ=255)
         aGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGcggcc                                                                                             <  1:3956106/101‑1 (MQ=255)
          gCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGGCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGcggccg                                                                                            >  1:3683098/1‑101 (MQ=255)
          gCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGcggccg                                                                                            <  2:3651888/101‑1 (MQ=255)
             aGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGGCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGGGgggccgggg                                                                                         >  1:468049/1‑99 (MQ=255)
               gTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGcgcg                                                                                       <  1:1121443/101‑1 (MQ=255)
               gTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGcgcg                                                                                       <  1:3003202/101‑1 (MQ=255)
                  cGCATCGCCGCGCCGGTGCGGCGGGGGGGGCCAAACCGCGGAGAGGAATAACCCTCGATGGCCAGCGTCGAATGGCTGGGGGGGGCGGGGCCCGGGCgggg                                                                                    >  2:4057133/1‑99 (MQ=255)
                   gCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGGCGAATGGCTGGGGGGGGCGCGGCCGGCGCggggg                                                                                   >  1:616690/1‑98 (MQ=255)
                    cATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGGGCGGGGcc                                                                                  >  1:2986481/1‑101 (MQ=255)
                          cgcgcggGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCgg                                                                            <  2:1201678/101‑1 (MQ=255)
                             gccgGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGGGCGGGGCCCGTCGGGGc                                                                         >  2:1766707/1‑101 (MQ=255)
                             gccgGGGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGc                                                                         <  2:30074/101‑1 (MQ=255)
                               cgGTGCGGGGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCg                                                                       <  2:660625/101‑1 (MQ=255)
                                ggTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGCGGGGGGCCCGGCGGGGGCGa                                                                      >  1:893159/1‑101 (MQ=255)
                                 gTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGaa                                                                     >  1:4504502/1‑101 (MQ=255)
                                    gggcgggcgGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACAt                                                                  <  2:2237347/100‑1 (MQ=255)
                                     ggcgggcgGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATc                                                                 <  1:413537/101‑1 (MQ=255)
                                     ggcgggcgGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATc                                                                 >  1:3348424/1‑101 (MQ=255)
                                     ggcgggcgGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATc                                                                 <  2:3912038/101‑1 (MQ=255)
                                        gggcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGcc                                                              <  1:3150354/101‑1 (MQ=255)
                                          gcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGcccc                                                            <  1:1808575/101‑1 (MQ=255)
                                          gcgggGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGcccc                                                            >  1:3215433/1‑101 (MQ=255)
                                          gcgggGCAAAGCCCGGACGAGAAATACCCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGGGGCCGGCGCGGTGCCAGTCGGGGCCGAAAATCGcccc                                                            <  2:858489/101‑1 (MQ=255)
                                           gggggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGccccc                                                           <  2:2625158/100‑1 (MQ=255)
                                            ggggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCg                                                          >  1:806083/1‑101 (MQ=255)
                                            ggggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCg                                                          >  2:4423368/1‑101 (MQ=255)
                                            ggggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCg                                                          >  2:2196518/1‑101 (MQ=255)
                                            ggggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCg                                                          >  2:408266/1‑101 (MQ=255)
                                              ggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGGCGGCGCGGTGCCCATCGGGGCCGGACATCGCCCCCggg                                                        >  1:2765630/1‑99 (MQ=255)
                                              ggCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAg                                                        >  2:2779120/1‑101 (MQ=255)
                                               gCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGc                                                       >  1:2930840/1‑101 (MQ=255)
                                                ccAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGcc                                                      <  1:1319143/101‑1 (MQ=255)
                                                 cAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCg                                                     >  2:3530465/1‑101 (MQ=255)
                                                 cAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGGGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCg                                                     <  2:4546988/101‑1 (MQ=255)
                                                   aGCCGCGACGAGGAATACCCCTCGATCGCCGGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGGGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCa                                                   <  2:3351798/101‑1 (MQ=255)
                                                   aGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCa                                                   <  2:2445855/101‑1 (MQ=255)
                                                   aGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCa                                                   <  1:770830/101‑1 (MQ=255)
                                                   aGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCa                                                   >  2:2644317/1‑101 (MQ=255)
                                                   aGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCCAGCCGCa                                                   >  2:1407954/1‑101 (MQ=255)
                                                    gCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCaa                                                  <  2:73232/101‑1 (MQ=255)
                                                    gCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCaa                                                  >  1:3098054/1‑101 (MQ=255)
                                                     ccGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAAt                                                 >  2:460504/1‑101 (MQ=255)
                                                       gcgACGAGGAATACCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTg                                               <  2:4237998/101‑1 (MQ=255)
                                                       gcgACGAGGAATACCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTg                                               <  1:266766/101‑1 (MQ=255)
                                                        cgaagaGGAATAACCCCCGAGCGCCAGCGTCAAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGa                                              <  2:616629/101‑1 (MQ=255)
                                                         gacgaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGAc                                             >  1:1524478/1‑101 (MQ=255)
                                                         gacgaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGAc                                             <  2:4655977/101‑1 (MQ=255)
                                                         gacgaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCAAGCCGCAATTGAc                                             >  2:767082/1‑101 (MQ=255)
                                                          acgaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGGCGCAATTGACg                                            >  2:3758706/1‑101 (MQ=255)
                                                           tgaGAAATGACCCACGATAGCAAGCCTCAAATGGCGGGGGGTGGCGCGGCCGGCGCGGTGCCGGTGGGGGCCGAACATCCCCCCCGAGCCGCAATTGACGa                                           <  2:1468507/100‑1 (MQ=255)
                                                            gaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGAt                                          >  2:1668446/1‑101 (MQ=255)
                                                            gaGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGAt                                          >  2:842737/1‑101 (MQ=255)
                                                             aGGAATAACCCTCGATCGCCGGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATc                                         <  1:4613963/101‑1 (MQ=255)
                                                              ggAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCa                                        >  2:2760829/1‑101 (MQ=255)
                                                               gAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAg                                       >  2:3582756/1‑101 (MQ=255)
                                                                 aTAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCg                                     >  1:2515716/1‑101 (MQ=255)
                                                                   aaCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGc                                   >  1:3513410/1‑101 (MQ=255)
                                                                   aaCCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGc                                   >  2:2498762/1‑101 (MQ=255)
                                                                     cccTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCa                                 <  1:3805279/101‑1 (MQ=255)
                                                                     cccTCGATCGCCAGCGTCGAAGGGCGGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCa                                 <  1:1673933/101‑1 (MQ=255)
                                                                       cTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAgc                               <  1:3546689/101‑1 (MQ=255)
                                                                        tCGATCGCGAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCAAACACCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAgcg                              <  2:2531104/101‑1 (MQ=255)
                                                                         cGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAgcgc                             >  2:4131257/1‑101 (MQ=255)
                                                                          gATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGcc                            >  2:492930/1‑101 (MQ=255)
                                                                            tCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGcccc                          <  2:3799343/101‑1 (MQ=255)
                                                                             cGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGGCCCg                         >  2:57750/1‑101 (MQ=255)
                                                                             cGCCAGCGGCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCg                         <  2:4487616/101‑1 (MQ=255)
                                                                              gCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGa                        >  2:1076472/1‑101 (MQ=255)
                                                                                cAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAgg                      >  2:3324589/1‑101 (MQ=255)
                                                                                cAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAgg                      >  2:2156541/1‑101 (MQ=255)
                                                                                 aGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAggg                     >  1:2424727/1‑100 (MQ=255)
                                                                                  gCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCTCAATTGACGATCAGCGGCCAGCGCCCCGAGGTg                    >  1:4015359/1‑101 (MQ=255)
                                                                                   cGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTgg                   >  2:2036930/1‑100 (MQ=255)
                                                                                   cGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGa                   >  1:1005017/1‑101 (MQ=255)
                                                                                    gTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAg                  >  1:2617184/1‑101 (MQ=255)
                                                                                     tCGAATGGCGGGGGGGGGCGCGGCCGGCGCGGTGCCGGTCGGGGCCAAACATCCCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGt                 <  1:2336636/101‑1 (MQ=255)
                                                                                      cGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGACCATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGtt                <  2:2675201/101‑1 (MQ=255)
                                                                                       gAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGGGAGTTc               >  1:1209585/1‑101 (MQ=255)
                                                                                        aaTGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGGGAGTTCa              >  2:1622453/1‑101 (MQ=255)
                                                                                         aTGGCGGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCAAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCaa             <  2:3821236/101‑1 (MQ=255)
                                                                                            gCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAgg          >  1:206762/1‑101 (MQ=255)
                                                                                            gCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAgg          >  1:1200545/1‑101 (MQ=255)
                                                                                            gCTGGGGGGGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAgg          <  1:3859027/101‑1 (MQ=255)
                                                                                             cTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGc         >  1:2289986/1‑101 (MQ=255)
                                                                                              tGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGcc        >  1:4306007/1‑101 (MQ=255)
                                                                                              tGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGcc        >  2:4215616/1‑101 (MQ=255)
                                                                                               gggggGGGCGGGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCa       <  2:3172253/101‑1 (MQ=255)
                                                                                               gggggGGGCGCGGCCGGCGGGGTGCCAGTCGGGGCCGAACATCCCCCCGGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCa       <  1:4480292/101‑1 (MQ=255)
                                                                                               gggggGGGCGCGGCCGGCGCGGTGCCAGGCGGGGCCGAACACCCCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCa       <  1:1364991/101‑1 (MQ=255)
                                                                                                ggggTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGGGAGTTCAAAGGCCAg      >  2:3254610/1‑101 (MQ=255)
                                                                                                  ggTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGCg    >  1:1068980/1‑101 (MQ=255)
                                                                                                    tGGCGCGGCGGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGCGTc  <  2:1524369/101‑1 (MQ=255)
                                                                                                    tGGCGCGGCCGGGGCGGTGCCAGTCGGGGCCGAACCTCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGCGTc  <  2:3796502/101‑1 (MQ=255)
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GTTTCCTCGAGCCAGGTCCGCATCGCCGCGCCGGTGCGGCGGGCGGGGCCAAGCCGCGACGAGGAATAACCCTCGATCGCCAGCGTCGAATGGCTGGGGGTGGCGCGGCCGGCGCGGTGCCAGTCGGGGCCGAACATCGCCCCCGAGCCGCAATTGACGATCAGCGGCCAGCGCCCCGAGGTGAGTTCAAAGGCCAGCGTC  >  NC_014034/310292‑310492

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG