Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 317,927 A→G 100% K338E (AAG→GAG)  RCAP_rcc00280 → hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034317,9270A→G100.0% 767.8 200K338E (AAG→GAG) RCAP_rcc00280hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (96/104):  ref base (0/0):  total (96/104)

Only 100 of 205 total aligned reads displayed.

AGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317830‑318024
                                                                                                 |                                                                                                 
aGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAgg                                                                                                <  1:1558549/101‑1 (MQ=255)
aGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAgg                                                                                                >  1:939491/1‑101 (MQ=255)
 gTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGa                                                                                               >  1:2474935/1‑101 (MQ=255)
 gTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGa                                                                                               >  2:4483126/1‑101 (MQ=255)
  tGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAt                                                                                              <  2:4258683/101‑1 (MQ=255)
   gACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtg                                                                                             <  2:4318175/101‑1 (MQ=255)
     cGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGTAtgtg                                                                                           <  2:393266/101‑1 (MQ=255)
     cGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtgtg                                                                                           <  1:4538972/101‑1 (MQ=255)
     cGCGGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGAtgtg                                                                                           <  1:4303671/101‑1 (MQ=255)
       cTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGct                                                                                         >  1:2149181/1‑101 (MQ=255)
        tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        <  1:2707009/101‑1 (MQ=255)
        tGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGCGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctc                                                                                        >  1:1710345/1‑101 (MQ=255)
         tcaGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGctct                                                                                       >  2:3136446/3‑101 (MQ=255)
          gAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCtt                                                                                      >  1:3134339/1‑101 (MQ=255)
           aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     >  1:1768965/1‑101 (MQ=255)
           aGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTg                                                                                     <  1:3750213/101‑1 (MQ=255)
             cccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGAt                                                                                   >  1:1969665/1‑101 (MQ=255)
              ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  2:2305607/101‑1 (MQ=255)
              ccGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  1:3393920/101‑1 (MQ=255)
              ccGCGACGACCGACGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATc                                                                                  <  1:4008357/101‑1 (MQ=255)
               cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  2:3461676/101‑1 (MQ=255)
               cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 <  1:1805365/101‑1 (MQ=255)
               cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 >  2:2619868/1‑101 (MQ=255)
               cgcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCa                                                                                 >  1:4045567/1‑101 (MQ=255)
                gcgACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCAc                                                                                <  2:3266327/101‑1 (MQ=255)
                  gacgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGc                                                                              >  2:4096627/1‑101 (MQ=255)
                  gacgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGc                                                                              <  2:2490457/101‑1 (MQ=255)
                  gacgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGc                                                                              >  2:2659227/1‑101 (MQ=255)
                   acgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGcc                                                                             <  1:1941191/101‑1 (MQ=255)
                    cgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGccc                                                                            <  1:646379/101‑1 (MQ=255)
                    cgacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGccc                                                                            <  1:3689145/101‑1 (MQ=255)
                     gacCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCg                                                                           >  2:843648/1‑101 (MQ=255)
                      acCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGa                                                                          >  2:3185399/1‑101 (MQ=255)
                      acCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGa                                                                          <  1:2279750/101‑1 (MQ=255)
                          aGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGaa                                                                      >  1:1083588/1‑101 (MQ=255)
                            ggCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAAtt                                                                    <  1:2424262/101‑1 (MQ=255)
                              cAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCg                                                                  >  1:4482745/1‑101 (MQ=255)
                              cAACGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCg                                                                  <  2:2918605/101‑1 (MQ=255)
                                aTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgc                                                                >  1:2675567/1‑101 (MQ=255)
                                aTGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgc                                                                >  1:164152/1‑101 (MQ=255)
                                 tGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcg                                                               <  1:4053995/101‑1 (MQ=255)
                                  gTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  2:507238/1‑101 (MQ=255)
                                  gTCTGGATCACGCTGAACGGGCTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGgcgc                                                              >  2:668922/1‑101 (MQ=255)
                                    cTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGccc                                                            <  2:2759874/101‑2 (MQ=255)
                                     tGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCAt                                                           >  1:950447/1‑101 (MQ=255)
                                     tGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCAATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCAt                                                           >  2:233796/1‑101 (MQ=255)
                                       gATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCt                                                         <  1:4210409/101‑1 (MQ=255)
                                        aTCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTGTTGATCACGCCCGAGGAATTCGGCGCCATCTg                                                        >  1:3246495/1‑101 (MQ=255)
                                         tCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGa                                                       <  2:2426752/101‑1 (MQ=255)
                                          cACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTgcc                                                      >  2:3587023/1‑99 (MQ=255)
                                          cACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAc                                                      >  1:3582819/1‑101 (MQ=255)
                                           aCGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGAcc                                                     <  1:119864/101‑1 (MQ=255)
                                            cGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCg                                                    <  2:4232314/101‑1 (MQ=255)
                                             gCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCgg                                                   >  2:1258713/1‑101 (MQ=255)
                                                 aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  2:2457382/101‑1 (MQ=255)
                                                 aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  1:666063/101‑1 (MQ=255)
                                                 aaCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCt                                               <  2:1989394/101‑1 (MQ=255)
                                                  aCGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTg                                              <  2:1919396/101‑1 (MQ=255)
                                                   cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             >  2:3431705/1‑101 (MQ=255)
                                                   cGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTgg                                             <  2:4103220/101‑1 (MQ=255)
                                                        tGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCtt                                        >  1:4445777/1‑101 (MQ=255)
                                                         gCATCTGGGCGAGGTGGTNGATGCGGGCGANCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       <  1:1213112/101‑1 (MQ=255)
                                                         gCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       <  2:571090/101‑1 (MQ=255)
                                                         gCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       >  1:3533073/1‑101 (MQ=255)
                                                         gCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTc                                       >  1:3876873/1‑101 (MQ=255)
                                                          cATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTcc                                      <  2:3414699/101‑1 (MQ=255)
                                                           aTCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTgcc                                     <  1:2659412/101‑1 (MQ=255)
                                                           aTCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTccc                                     <  2:2582987/101‑1 (MQ=255)
                                                            tCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCt                                    <  2:207939/101‑1 (MQ=255)
                                                             cTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCtt                                   <  2:143034/101‑1 (MQ=255)
                                                              tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:383479/101‑1 (MQ=255)
                                                              tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  >  2:1699803/1‑101 (MQ=255)
                                                              tGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCttt                                  <  2:1354431/101‑1 (MQ=255)
                                                                gCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                >  1:1099570/1‑101 (MQ=255)
                                                                gCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                <  2:3765757/101‑1 (MQ=255)
                                                                gCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGc                                <  1:843724/101‑1 (MQ=255)
                                                                gCCGAGGTGGTCGATGCGGGCGACCTGCGCCCCGAGGATGTGCTCTTGTTCACTCCCGAGGAATTCGCACCCATGTGACCGGGCCTCGGGCTTCCCtttcc                                >  2:730196/1‑99 (MQ=255)
                                                                 ccGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGcc                               <  2:1768846/101‑1 (MQ=255)
                                                                  cGAGGTGGTCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGccc                              <  1:549773/101‑1 (MQ=255)
                                                                       tggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtt                         >  1:2918827/1‑101 (MQ=255)
                                                                        ggtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCttt                        >  2:1742247/1‑101 (MQ=255)
                                                                         gtCGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCtttt                       <  2:1658349/101‑1 (MQ=255)
                                                                           cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  1:3274009/1‑101 (MQ=255)
                                                                           cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  1:3755838/1‑101 (MQ=255)
                                                                           cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     <  2:4284023/101‑1 (MQ=255)
                                                                           cGATGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTcc                     >  2:4584663/1‑101 (MQ=255)
                                                                             aTGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGc                   >  2:3649902/1‑101 (MQ=255)
                                                                              tGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCt                  <  2:3876588/101‑1 (MQ=255)
                                                                              tGCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCt                  >  2:3250917/1‑101 (MQ=255)
                                                                               tcGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 <  2:487887/100‑1 (MQ=255)
                                                                               gCGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTa                 <  1:3875291/101‑1 (MQ=255)
                                                                                cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                <  2:2028191/101‑1 (MQ=255)
                                                                                cGGGCGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTaa                <  2:303996/101‑1 (MQ=255)
                                                                                    cGATCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgc            <  2:3996246/101‑1 (MQ=255)
                                                                                      aTCTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAAcgccg          <  2:3471103/101‑1 (MQ=255)
                                                                                        cTGCGCCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACgccgcc        >  2:2544413/1‑101 (MQ=255)
                                                                                           cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  2:3566996/1‑101 (MQ=255)
                                                                                           cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  1:1322641/1‑101 (MQ=255)
                                                                                           cgcCCCGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATc     >  1:1007626/1‑101 (MQ=255)
                                                                                             ccccGAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATccg   >  2:3899488/1‑101 (MQ=255)
                                                                                                 |                                                                                                 
AGTGACGCTGGAGCCCGCGACGACCGAGGGCAATGTCTGGATCACGCTGAACGGGGTGCATCTGGCCGAGGTGGTCGATGCGGGCGATCTGCGCCCCAAGGATGTGCTCTTGATCACGCCCGAGGAATTCGGCGCCATCTGACCGGGCCTGGGGCTTCCCTTTGCCCCGCTTTTCCGCTAAACGCCGCCATCCGC  >  NC_014034/317830‑318024

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG