Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,694 A→C 100% intergenic (+19/‑164) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6940A→C100.0% 401.5 120intergenic (+19/‑164)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (7/107):  ref base (1/0):  total (13/107)

Only 100 of 137 total aligned reads displayed.

GACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAGG  >  NC_014034/3254603‑3254788
                                                                                            |                                                                                              
  ccGAAACTATCCTGATGCTCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                                    <  2:1710965/101‑1 (MQ=255)
  ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                                    <  1:3350768/101‑1 (MQ=255)
  ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                                    <  2:3816587/101‑1 (MQ=255)
       aCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccc                                                                               <  1:4470561/101‑1 (MQ=255)
         gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGG.CGGCCGTCCCCCCCCccccc                                                                             >  2:2218721/1‑101 (MQ=255)
         gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccccc                                                                             <  2:2041516/101‑1 (MQ=255)
         gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccccc                                                                             <  1:4330502/101‑1 (MQ=255)
          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCCGCCCCCCCCCccccc                                                                             >  2:1499475/1‑101 (MQ=255)
          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCt                                                                            <  2:4216991/101‑1 (MQ=255)
          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCt                                                                            <  1:271363/101‑1 (MQ=255)
           tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGCCGCCCCCCCCCccccccc                                                                           >  1:2597086/1‑99 (MQ=255)
           tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                                           <  2:2411971/101‑1 (MQ=255)
           tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                                           <  2:4542852/101‑1 (MQ=255)
            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCAGGGGGCAAGCGCCCCCCCCCCcccccc                                                                            >  1:2613409/1‑100 (MQ=255)
            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgc                                                                          <  2:447101/101‑1 (MQ=255)
            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgc                                                                          <  1:3816714/101‑1 (MQ=255)
            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgc                                                                          <  2:197452/101‑1 (MQ=255)
              tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAGCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgcgc                                                                        <  1:2914053/101‑1 (MQ=255)
                aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGt                                                                      <  1:1680299/101‑1 (MQ=255)
                aTGCGCGCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGt                                                                      <  2:247362/101‑1 (MQ=255)
                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTTTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAt                                                                    <  2:4641444/101‑1 (MQ=255)
                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAt                                                                    <  1:584128/101‑1 (MQ=255)
                   cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGCGGGCCGCCCCCCCCCcccccccccggctt                                                                    >  2:397855/1‑92 (MQ=255)
                   cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtt                                                                   <  1:2896655/101‑1 (MQ=255)
                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAttt                                                                  <  1:1475072/101‑1 (MQ=255)
                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                                 <  1:4389662/101‑1 (MQ=255)
                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                                 <  2:2152859/101‑1 (MQ=255)
                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                                 <  2:1344829/101‑1 (MQ=255)
                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                                 <  2:1160657/101‑1 (MQ=255)
                      tCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTg                                                                <  1:4244211/101‑1 (MQ=255)
                       cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGc                                                               <  2:2029194/101‑1 (MQ=255)
                       cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGc                                                               <  2:1269550/101‑1 (MQ=255)
                        gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCACCCCCCGCGCCGATTTTGcc                                                              >  2:1330090/1‑101 (MQ=255)
                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                             <  1:2779244/101‑1 (MQ=255)
                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                             <  1:4569194/101‑1 (MQ=255)
                          aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAGCGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCaa                                                            <  2:2095972/101‑1 (MQ=255)
                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAg                                                           <  1:973065/101‑1 (MQ=255)
                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAg                                                           <  1:1087342/101‑1 (MQ=255)
                              cGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaa                                                        <  1:3502654/101‑1 (MQ=38)
                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                                       <  1:4042744/101‑1 (MQ=38)
                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                                       <  2:373749/101‑1 (MQ=38)
                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                                       <  1:1499584/101‑1 (MQ=38)
                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                                      <  1:600877/101‑1 (MQ=255)
                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                                      <  1:2325939/101‑1 (MQ=38)
                                gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                                      <  2:1027475/101‑1 (MQ=38)
                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCCCGCGGCTTTTTCCCAGGAAAACgggg                                                 >  1:1343865/1‑101 (MQ=255)
                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACgggg                                                 <  2:1793684/101‑1 (MQ=38)
                                       gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGcc                                               <  2:17358/101‑1 (MQ=38)
                                        cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                              >  2:712934/1‑101 (MQ=38)
                                        cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                              <  1:2916078/101‑1 (MQ=38)
                                        atctgggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                              <  1:115402/97‑1 (MQ=38)
                                         gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCGGCCCCCCCACCCCCCGCGCGGATTTTGCCCAGGAAAAAGGGGGCgg                                             >  2:654668/1‑100 (MQ=255)
                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGCGGGG.CCGGCGCCCCCCCCACCCCCCTCGCCGAGTTTGGCCACGAAAAAGGGGGCgct                                            >  1:1528656/1‑99 (MQ=255)
                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACggggacaac                                            <  2:2440448/101‑6 (MQ=38)
                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGtt                                            <  2:4110317/101‑1 (MQ=38)
                                           cGGGTGATCGATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGttt                                           <  2:3889468/101‑1 (MQ=38)
                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGCCGCCCCCCCCCCCCCCCCCGCGCGTTTTTCCAAGGAAAAAAGGGCCGCTTc                                          >  1:3089753/1‑101 (MQ=255)
                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGGCGCCCCCCCCCCCCCCCCCGCGTATTTTGCCAAGGAAAACCGGGGCGCTTc                                          >  1:2296644/1‑101 (MQ=16)
                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGTCCCCCCCCCCCCCCGCGCGGCTTTTTTCAAGGAAAAAAGGGCCGGTTCAc                                        >  2:2663691/1‑101 (MQ=255)
                                              gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGGCCCCCCCCCCCCCCCCGCGTATTTTGTCCAAGAAAAAAGGGCCGGTTCAc                                        >  2:1759167/1‑101 (MQ=255)
                                               tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCGGCCCCCCCCCCCCCCCCCCGGGTTTTTTCCCAGGAAAAAGGGGcgggtttcc                                        >  1:1152305/1‑93 (MQ=255)
                                                gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCGCACCCCCCGCGCGGATTTTGCCAAGGAAAACAGGGGCGGTTTACCt                                      >  2:2931137/1‑101 (MQ=21)
                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                                     <  2:1728282/101‑1 (MQ=38)
                                                 aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                                     <  1:2660925/101‑1 (MQ=38)
                                                 aTCTATCGCAACACGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                                     <  2:3898496/101‑1 (MQ=38)
                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                                    <  2:281610/101‑1 (MQ=38)
                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                                    <  2:2732809/101‑1 (MQ=38)
                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                                    <  2:4287794/101‑1 (MQ=38)
                                                   cTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTgg                                   <  1:3381775/101‑1 (MQ=38)
                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGcc                                 <  2:3679341/101‑1 (MQ=38)
                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGcc                                 <  1:4475684/101‑1 (MQ=38)
                                                      tccgaaCCCGAGCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  1:3160366/97‑1 (MQ=37)
                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  1:2303984/101‑1 (MQ=38)
                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                                <  2:106369/101‑1 (MQ=38)
                                                       cNNAACACGATCCGCTGNGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                               <  2:1908779/101‑1 (MQ=37)
                                                         cAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGaaa                             <  2:4242971/101‑1 (MQ=38)
                                                          aaCCCGATCCGCTGAGGNAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                            <  2:387979/101‑1 (MQ=38)
                                                          aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                            <  1:4568001/101‑1 (MQ=38)
                                                           aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCCCCCCCCGCGCGTATTTTGCCAAAGACAACGGGGGCGGTTCACCTTGGCCGACata                           >  2:2094577/1‑101 (MQ=255)
                                                           aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAata                           <  1:3526241/101‑1 (MQ=38)
                                                              cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATcc                        <  2:2454920/101‑1 (MQ=38)
                                                               gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATccc                       <  1:4503256/101‑1 (MQ=38)
                                                                aTCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGGCCCCCCCCCCCCCCCCCCGGCTTTTTTCAAAGAAAAAGGGGGCCGTTCACCCTTGCCCAAAAATcccc                      >  1:3874183/1‑100 (MQ=255)
                                                                aTCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAGATATCCCg                      <  1:1371091/101‑1 (MQ=38)
                                                                aTCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCg                      <  2:328875/101‑1 (MQ=38)
                                                                    gCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCggggg                  <  2:2264685/101‑1 (MQ=38)
                                                                     cTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgggggg                 <  1:3896625/101‑1 (MQ=38)
                                                                        aGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGaa              <  1:4600983/101‑1 (MQ=38)
                                                                               acgctacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGc         <  2:3465050/94‑1 (MQ=38)
                                                                                cgctacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACTGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGcc        <  2:2400260/95‑1 (MQ=38)
                                                                                cgctacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGcc        <  1:1962636/95‑1 (MQ=38)
                                                                                cgctacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGcc        <  2:1343770/95‑1 (MQ=38)
                                                                                 gctacgggggGCTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGGATTTGCCg       >  2:2859617/6‑101 (MQ=38)
                                                                                  ctacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGa      <  1:4358512/97‑1 (MQ=255)
                                                                                   tacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGAc     >  2:1488021/4‑101 (MQ=255)
                                                                                   tacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGAc     <  1:3421976/98‑1 (MQ=255)
                                                                                   tacgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGAc     <  2:1247304/98‑1 (MQ=255)
                                                                                    acgggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACa    <  1:1607892/99‑1 (MQ=255)
                                                                                     tgggggACTGCCGGCCCCCGCGCCTCCGGCGCGTGGTTTGCCAAGGAAAACGGGGCCGTTGCACCTTGGCCGAAATATCCCGGGGGGTAATTTGCCGACAg   <  2:2647447/100‑1 (MQ=25)
                                                                                      gggggACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAgg  <  1:3041579/101‑1 (MQ=255)
                                                                                            |                                                                                              
GACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAATTTGCCGACAGG  >  NC_014034/3254603‑3254788

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG