Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,880 G→A 100% P228L (CCG→CTG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,8800G→A100.0% 975.2 258P228L (CCG→CTG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (122/136):  ref base (0/0):  total (122/136)

Only 100 of 270 total aligned reads displayed.

CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977
                                                                                                  |                                                                                                 
   cATGTTTGCCCTTGCCTCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  2:168980/101‑1 (MQ=255)
   cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  1:2965865/101‑1 (MQ=255)
   cATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATg                                                                                              <  1:4271288/101‑1 (MQ=255)
    aTGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCTCCCATCGCTTACGCGTCCAGCTCGCGCGACAGGATgg                                                                                             >  2:3122969/1‑101 (MQ=255)
    aTGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATgg                                                                                             <  1:2535584/101‑1 (MQ=255)
    aTGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATgg                                                                                             >  1:2144501/1‑101 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           <  2:4492882/101‑1 (MQ=255)
      gTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTc                                                                                           <  2:1638931/101‑1 (MQ=255)
        ttGCCGTTGCCGACGCGGCGCCGCCGCTTGCCGGCGCCTCTCTTTTTCATCACCCGCGCGTTCATCGTGTGCGGGTCCAGCTGGCGCGACAGGAAGGTCga                                                                                         <  2:4389684/101‑1 (MQ=255)
          gCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCgaga                                                                                       >  1:4587570/1‑101 (MQ=255)
           cccTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGaa                                                                                      <  2:4277946/101‑1 (MQ=255)
            ccTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAt                                                                                     >  2:2617200/1‑101 (MQ=255)
             cTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTccgggatt                                                                                    >  2:2175096/1‑94 (MQ=255)
             cTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAAtt                                                                                    <  2:1715587/101‑1 (MQ=255)
              ttGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtc                                                                                   <  1:1949496/101‑1 (MQ=255)
               tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  >  1:630678/1‑101 (MQ=255)
               tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  >  2:2439900/1‑101 (MQ=255)
               tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  <  2:2394006/101‑1 (MQ=255)
               tGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcg                                                                                  <  2:765268/101‑1 (MQ=255)
                gccgccGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgt                                                                                 >  1:3307488/1‑101 (MQ=255)
                 ccgccgCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATtcgtc                                                                                <  1:4121186/101‑1 (MQ=255)
                   gccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCgg                                                                              >  1:2599737/1‑101 (MQ=255)
                   gccgcGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGGCGAGAATTCGTCgg                                                                              >  2:1601283/1‑101 (MQ=255)
                     cgcgGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCg                                                                            >  1:2158741/1‑101 (MQ=255)
                     cgcgGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCg                                                                            >  1:3028033/1‑101 (MQ=255)
                       cggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGaa                                                                          <  2:319368/101‑1 (MQ=255)
                        ggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAGTTCGTCGGCGAAg                                                                         >  1:4011214/1‑101 (MQ=255)
                        ggcggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAg                                                                         >  2:340561/1‑101 (MQ=255)
                           ggcgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGcc                                                                      >  1:468651/1‑101 (MQ=255)
                             cgTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTc                                                                    >  2:2135842/1‑101 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 >  1:1140034/1‑101 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:1470709/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:1622865/101‑1 (MQ=255)
                                cGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTa                                                                 <  1:3590449/101‑1 (MQ=255)
                                 ggCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAg                                                                >  2:3506483/1‑101 (MQ=255)
                                 ggCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAg                                                                >  1:424380/1‑101 (MQ=255)
                                   cTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGGGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGcc                                                              >  2:4027753/1‑101 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGGATTCGTCGACGAAGGCCTCGTAGccc                                                             >  1:3919036/1‑101 (MQ=255)
                                    tGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGccc                                                             >  2:524275/1‑101 (MQ=255)
                                     gccgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCg                                                            >  2:695681/1‑101 (MQ=255)
                                       cgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAg                                                          >  2:1864650/1‑101 (MQ=255)
                                       cgccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAg                                                          <  2:2706013/101‑1 (MQ=255)
                                        gccTCCGCCCTTTTTCATCCCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAgg                                                         <  1:734169/101‑1 (MQ=255)
                                        gccTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAgg                                                         >  2:316973/1‑101 (MQ=255)
                                          cTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTg                                                       <  2:4010929/101‑1 (MQ=255)
                                           tCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGa                                                      >  2:2237837/1‑101 (MQ=255)
                                             cGCCCTTTTTCATCACCCCCGCGCCCATCGCGTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGAtc                                                    <  1:3717347/101‑1 (MQ=255)
                                                 cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgt                                                <  2:1778190/101‑1 (MQ=255)
                                                 cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCttgt                                                >  2:656423/1‑101 (MQ=255)
                                                  tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                               >  1:1569309/1‑101 (MQ=255)
                                                   ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                              <  1:1015201/101‑1 (MQ=255)
                                                      tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcg                                           >  2:679638/1‑101 (MQ=255)
                                                      tcatcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgccggcg                                           >  1:4408727/1‑101 (MQ=255)
                                                       catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                          >  1:814655/1‑101 (MQ=255)
                                                        atcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtc                                         >  2:464899/1‑101 (MQ=255)
                                                            cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                     <  1:3187328/101‑1 (MQ=255)
                                                            cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                     <  1:2385967/101‑1 (MQ=255)
                                                            cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                     >  2:1574543/1‑101 (MQ=255)
                                                             ccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACg                                    >  1:211631/1‑101 (MQ=255)
                                                              cccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACga                                   >  1:506054/1‑101 (MQ=255)
                                                              cccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACga                                   >  2:1248679/1‑101 (MQ=255)
                                                                 gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                <  1:4041956/101‑1 (MQ=255)
                                                                 gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                >  1:3600364/1‑101 (MQ=255)
                                                                   gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                              >  1:695239/1‑101 (MQ=255)
                                                                   gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                              <  1:1805662/101‑1 (MQ=255)
                                                                     ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                            <  2:1154028/101‑1 (MQ=255)
                                                                     ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGGGAGCttg                            >  1:3456715/1‑101 (MQ=255)
                                                                      cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                           >  2:4347796/1‑101 (MQ=255)
                                                                      cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                           >  1:966038/1‑101 (MQ=255)
                                                                       aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCTTGTCGGCGTCGGACGAGAGCttgtt                          >  2:364104/1‑101 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        >  1:2757902/1‑101 (MQ=255)
                                                                         cGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTtgttgt                        >  1:1355439/1‑101 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:3271381/101‑1 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      >  2:1544410/1‑101 (MQ=255)
                                                                           cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                      <  1:1601388/101‑1 (MQ=255)
                                                                            ttACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAgg                     >  2:3462382/1‑101 (MQ=255)
                                                                              aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                   >  2:4206917/1‑101 (MQ=255)
                                                                              aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                   >  2:710304/1‑101 (MQ=255)
                                                                                 cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  2:2334929/101‑1 (MQ=255)
                                                                                 cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  1:2243700/101‑1 (MQ=255)
                                                                                 cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGACGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                <  2:3918751/101‑1 (MQ=255)
                                                                                  gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa               <  2:3027804/101‑1 (MQ=255)
                                                                                  gTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGa               <  1:607392/101‑1 (MQ=255)
                                                                                   tCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:1133476/101‑1 (MQ=255)
                                                                                   tCCAGCGGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc              <  2:881945/101‑1 (MQ=255)
                                                                                    ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc             >  2:4198872/1‑101 (MQ=255)
                                                                                       gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc          <  2:1889394/101‑1 (MQ=255)
                                                                                       gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc          >  2:4095855/1‑101 (MQ=255)
                                                                                       gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc          <  2:498480/101‑1 (MQ=255)
                                                                                        cTGGGGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  2:3205798/101‑1 (MQ=255)
                                                                                        cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg         <  1:1168969/101‑1 (MQ=255)
                                                                                         tGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCgg        <  1:4506700/101‑1 (MQ=255)
                                                                                          ggCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCggg       >  1:820891/1‑101 (MQ=255)
                                                                                            cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt     <  2:1017575/101‑1 (MQ=255)
                                                                                            cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt     <  1:4334000/101‑1 (MQ=255)
                                                                                            cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt     <  1:227689/101‑1 (MQ=255)
                                                                                             gcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATc    <  1:679700/101‑1 (MQ=255)
                                                                                              cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg   >  2:611644/1‑101 (MQ=255)
                                                                                               gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc  <  1:3376809/101‑1 (MQ=255)
                                                                                               gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc  >  2:844885/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CGGCATGTTTGCCCTTGCCGCCGCGGCGGCGTCGGCTGCCGCCTCCGCCCTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGC  >  NC_014034/3398782‑3398977

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG