Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,398,929 C→T 100% E212K (GAG→AAG)  tolQ ← TolQ protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,398,9290C→T100.0% 871.8 221E212K (GAG→AAG) tolQTolQ protein
Reads supporting (aligned to +/- strand):  new base (107/114):  ref base (0/0):  total (107/114)

Only 100 of 253 total aligned reads displayed.

CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026
                                                                                                  |                                                                                                 
cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGGGAAGGCCTCGTAGCCCGAGGTGATCttgt                                                                                                 >  2:853578/1‑101 (MQ=255)
cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgt                                                                                                 <  1:4112488/101‑1 (MQ=255)
cTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgt                                                                                                 <  1:4598263/101‑1 (MQ=255)
 tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                <  1:2884245/101‑1 (MQ=255)
 tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtc                                                                                                >  1:1569309/1‑101 (MQ=255)
 tttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGGGATCttgtc                                                                                                >  2:1588260/1‑101 (MQ=255)
  ttttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCttgtcg                                                                                               <  1:1015201/101‑1 (MQ=255)
    ttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             <  1:464194/101‑1 (MQ=255)
    ttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             >  1:1142885/1‑101 (MQ=255)
    ttCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggc                                                                                             >  2:2008093/1‑101 (MQ=255)
      catcaCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgt                                                                                           >  1:814655/1‑101 (MQ=255)
         caCCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTtgtcggcgtcgg                                                                                        <  1:4220986/101‑1 (MQ=255)
          ccccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGa                                                                                       <  1:2134096/100‑1 (MQ=255)
           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      <  1:2275791/101‑1 (MQ=255)
           cccccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGAc                                                                                      <  1:2385967/101‑1 (MQ=255)
             cccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACga                                                                                    >  1:506054/1‑101 (MQ=255)
             cccGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACga                                                                                    >  2:1248679/1‑101 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  1:3159865/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  2:814581/101‑1 (MQ=255)
               cgcgCCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACgaga                                                                                  <  2:1682677/101‑1 (MQ=255)
                gcgcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGagag                                                                                 <  1:4041956/101‑1 (MQ=255)
                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGACGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               >  1:2149240/1‑101 (MQ=255)
                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               >  2:2337051/1‑101 (MQ=255)
                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               <  2:2738855/101‑1 (MQ=255)
                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               >  1:695239/1‑101 (MQ=255)
                  gcCCATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCt                                                                               <  1:1805662/101‑1 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             <  2:1154028/101‑1 (MQ=255)
                    ccATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttg                                                                             <  1:697294/101‑1 (MQ=255)
                     cATCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgt                                                                            <  1:4366129/101‑1 (MQ=255)
                      aTCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgtt                                                                           >  2:676796/1‑101 (MQ=255)
                       tCGCTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCttgttg                                                                          >  1:4173921/1‑101 (MQ=255)
                          cTTACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAg                                                                       <  1:3271381/101‑1 (MQ=255)
                            tACGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGc                                                                     >  1:3592146/1‑101 (MQ=255)
                             aCGCGTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCg                                                                    >  2:710304/1‑101 (MQ=255)
                              cgcgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGa                                                                   >  1:4494881/1‑101 (MQ=255)
                                cgTCCAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGACGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATg                                                                 <  2:3918751/101‑1 (MQ=255)
                                  tCCAGCGGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAc                                                               <  2:881945/101‑1 (MQ=255)
                                   ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc                                                              <  1:3380245/101‑1 (MQ=255)
                                   ccAGCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAcc                                                              >  2:4198872/1‑101 (MQ=255)
                                      gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           <  2:498480/101‑1 (MQ=255)
                                      gCTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgcc                                                           <  2:317524/101‑1 (MQ=255)
                                       cTGGGGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  2:3205798/101‑1 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  1:379500/101‑1 (MQ=255)
                                       cTGGCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGAccgccg                                                          <  2:1569206/101‑1 (MQ=255)
                                         ggCGCGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCggg                                                        >  1:820891/1‑101 (MQ=255)
                                          gcgcGACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGa                                                       <  1:3350301/101‑1 (MQ=255)
                                           cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCTTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt                                                      <  2:3456461/101‑1 (MQ=255)
                                           cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt                                                      <  2:1740641/101‑1 (MQ=255)
                                           cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt                                                      <  2:2144351/101‑1 (MQ=255)
                                           cgcgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGAt                                                      <  2:1017575/101‑1 (MQ=255)
                                             cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg                                                    >  2:611644/1‑101 (MQ=255)
                                             cgACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcg                                                    <  1:2237999/101‑1 (MQ=255)
                                              gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc                                                   <  1:2699412/101‑1 (MQ=255)
                                              gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc                                                   <  1:1585036/101‑1 (MQ=255)
                                              gACAGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgc                                                   >  2:2641662/1‑101 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  2:4453775/4‑101 (MQ=255)
                                               acaggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATcgcg                                                  >  2:252778/4‑101 (MQ=255)
                                                caggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcgg                                                 >  2:65077/3‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCTAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                >  1:2324947/2‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                >  2:4510906/2‑101 (MQ=255)
                                                 aggATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                >  1:3429817/2‑101 (MQ=255)
                                                 aggATGGTCGAGAATGCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCgcggc                                                <  2:468603/100‑1 (MQ=255)
                                                   gATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGa                                              >  2:3775735/1‑101 (MQ=255)
                                                    aTGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGAc                                             <  1:364141/101‑1 (MQ=255)
                                                      ggTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACaa                                           >  1:1388520/1‑101 (MQ=255)
                                                      ggTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACaa                                           <  1:1241356/101‑1 (MQ=255)
                                                      ggTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACaa                                           <  2:2166584/101‑1 (MQ=255)
                                                       gTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAg                                          <  1:647780/101‑1 (MQ=255)
                                                             aaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    <  1:1868647/101‑1 (MQ=255)
                                                             aaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    <  2:3601945/101‑1 (MQ=255)
                                                             aaTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGAt                                    >  2:4044096/1‑101 (MQ=255)
                                                              aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATc                                   >  1:3109426/1‑101 (MQ=255)
                                                              aTTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATc                                   <  2:4311107/101‑1 (MQ=255)
                                                                 cgtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGcc                                <  2:3583229/101‑1 (MQ=255)
                                                                  gtcgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCg                               >  1:2720543/1‑101 (MQ=255)
                                                                    cgGCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTg                             >  2:1458196/1‑101 (MQ=255)
                                                                     ggCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTgg                            >  2:2311220/1‑101 (MQ=255)
                                                                     ggCGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTgg                            >  2:1822699/1‑101 (MQ=255)
                                                                       cGAAGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGcc                          >  2:3670113/1‑101 (MQ=255)
                                                                         aaGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        <  1:3982765/101‑1 (MQ=255)
                                                                         aaGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccac                        >  1:3474323/1‑101 (MQ=255)
                                                                          aGGCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacc                       <  1:676858/101‑1 (MQ=255)
                                                                           ggCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacca                      <  1:1701977/101‑1 (MQ=255)
                                                                           ggCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccacca                      >  2:4665972/1‑101 (MQ=255)
                                                                            gCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGccaccac                     >  2:4082300/1‑100 (MQ=255)
                                                                            gCCTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAg                     >  2:2915539/1‑101 (MQ=255)
                                                                             ccTCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgc                    <  2:4259981/101‑1 (MQ=255)
                                                                               tCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgcgc                  >  1:321482/1‑101 (MQ=255)
                                                                               tCGTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAgcgc                  >  1:2023751/1‑101 (MQ=255)
                                                                                 gTAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCt                >  1:4315139/1‑101 (MQ=255)
                                                                                  tAGCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTc               <  2:1139945/101‑1 (MQ=255)
                                                                                    gCCCGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTTTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCgg             >  1:3573474/1‑101 (MQ=255)
                                                                                      ccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgag           >  1:2964734/1‑101 (MQ=255)
                                                                                      ccGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgag           <  2:695177/101‑1 (MQ=255)
                                                                                       cGAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGgaga          >  1:2514029/1‑101 (MQ=255)
                                                                                        gAGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGAt         <  2:1355344/101‑1 (MQ=255)
                                                                                         aGGTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATc        >  2:3313770/1‑101 (MQ=255)
                                                                                           gTGATCTTGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATccc      >  2:348137/1‑101 (MQ=255)
                                                                                            tGATCTTGTCGGCGTCGGACGAGAGCTGGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATcccc     <  2:2149090/101‑1 (MQ=255)
                                                                                               tcttGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGgc  >  2:2869836/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTTTTTCATCACCCCCGCGCCCATCGCTTACGCGTCCAGCTGGCGCGACGGGATGGTCGAGAATTCGTCGGCGAAGGCCTCGTAGCCCGAGGTGATCTCGTCGGCGTCGGACGAGAGCTTGTTGTAGGCGATGACCGCCGGGATCGCGGCGACAAGGCCGATCGCCGTGGCCACCAGCGCCTCGGAGATCCCCGGC  >  NC_014034/3398831‑3399026

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG