Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,604 T→C 100% A410A (GCT→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6040T→C100.0% 890.8 236A410A (GCT→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (129/107):  ref base (0/0):  total (129/107)

Only 100 of 260 total aligned reads displayed.

CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531506‑3531701
                                                                                                  |                                                                                                 
cTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTa                                                                                                 <  2:4327761/101‑1 (MQ=255)
   aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                              <  1:2424636/101‑1 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                             <  2:1056984/101‑1 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                             >  2:3231773/1‑101 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            >  1:3221797/1‑101 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            <  1:437848/101‑1 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            <  1:2080815/101‑1 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            >  1:1936522/1‑101 (MQ=255)
      tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           <  2:3474980/101‑1 (MQ=255)
      tACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGaa                                                                                           >  2:4521685/1‑101 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                          >  2:4454754/1‑101 (MQ=255)
        caacaaCTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACg                                                                                         <  2:4139600/101‑1 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        <  1:2253119/101‑1 (MQ=255)
          acaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGACCggg                                                                                       >  2:3274516/1‑101 (MQ=255)
          acaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACggg                                                                                       >  2:4671312/1‑101 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGccc                                                                                    <  2:4535966/101‑1 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGccc                                                                                    <  2:1466859/101‑1 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGccc                                                                                    <  1:4652048/101‑1 (MQ=255)
              cTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCg                                                                                   >  1:585363/1‑101 (MQ=255)
               tCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  1:2163330/101‑1 (MQ=255)
               tCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  2:2124936/101‑1 (MQ=255)
               ccGCCCAGCTTCAACTGGACGCTGACCCTGCGCAAGCAGGTGCGCGCGCAGGGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  1:3808125/100‑1 (MQ=255)
                cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAg                                                                                 >  1:2507430/1‑101 (MQ=255)
                 gCCCAGCTTCAACTGGACGCTGAACCTGCGCAATCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGc                                                                                <  1:3967501/101‑1 (MQ=255)
                  cccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCt                                                                               <  1:1113026/101‑1 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                              >  2:4068049/1‑101 (MQ=255)
                    cAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtga                                                                             >  1:2911273/1‑101 (MQ=255)
                    cAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtga                                                                             >  1:1067401/1‑101 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTTGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                           <  1:433213/101‑1 (MQ=255)
                       cTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatga                                                                          >  1:2373137/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                         >  1:4159068/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                         >  2:4063642/1‑101 (MQ=255)
                          cAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGc                                                                       <  2:2257788/101‑1 (MQ=255)
                           aaCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGcc                                                                      >  1:1452419/1‑101 (MQ=255)
                            aCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                     >  1:2770719/1‑101 (MQ=255)
                            aCTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGccc                                                                     <  1:4431213/101‑1 (MQ=255)
                             cTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCg                                                                    <  1:1391150/101‑1 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGa                                                                   <  2:2686530/101‑1 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGa                                                                   <  2:253143/101‑1 (MQ=255)
                              tGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGACCGa                                                                   >  2:348948/1‑101 (MQ=255)
                               ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAc                                                                  >  1:1495531/1‑101 (MQ=255)
                                 aCGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTa                                                                >  2:507251/1‑101 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGGGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATga                                                             >  1:1370329/1‑101 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            >  1:4109635/1‑101 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGCGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACCGGCCCGAGCCGATGAAGCCCGACTATgcc                                                            >  2:1788873/1‑99 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  2:1214544/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           <  2:2816791/101‑1 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  2:3393234/1‑101 (MQ=255)
                                        aCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacgac                                                         >  2:2013046/1‑101 (MQ=255)
                                         ccTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGaca                                                        >  1:1038027/1‑101 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                       >  2:821405/1‑101 (MQ=255)
                                          cTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGacac                                                       >  1:3538698/1‑101 (MQ=255)
                                           tGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACCATGACGACCCg                                                      >  1:566351/1‑101 (MQ=255)
                                             cgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcc                                                    >  2:611109/1‑101 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  >  1:881377/1‑100 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                 <  2:2855613/101‑3 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                 <  2:494215/101‑3 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCCAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccccc                                                 >  2:2430988/1‑99 (MQ=255)
                                                aaGCAGGGGCGCGCGCAGTGGCTGGCNNAGNGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                 <  1:3109940/101‑3 (MQ=255)
                                                  gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                               >  1:3486527/1‑101 (MQ=255)
                                                   cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                              <  1:2325212/101‑1 (MQ=255)
                                                   cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                              >  1:675976/1‑101 (MQ=255)
                                                    aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                             <  2:1098642/101‑1 (MQ=255)
                                                    aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                             >  1:99891/1‑101 (MQ=255)
                                                      gTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcg                                           >  2:1928489/1‑101 (MQ=255)
                                                          gcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGaa                                       <  1:1590420/101‑1 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCCCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGTTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                      <  2:1413695/101‑1 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                      <  1:3196042/101‑1 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                      <  1:4068335/101‑1 (MQ=255)
                                                               cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                  <  1:119029/101‑1 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCCCGAGCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACCCGCCCCTCGGCCGCGAAGCCGAt                                 <  2:2899839/101‑1 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGccaga                                 <  2:2695268/101‑4 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 >  1:1980113/1‑101 (MQ=255)
                                                                    gctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                             <  1:4063928/101‑3 (MQ=255)
                                                                    gctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                             >  2:3874772/1‑99 (MQ=255)
                                                                     gtggcCGAGGGCACGATCGCGGAAGACGCCTATCCGAAGGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            <  2:1673379/100‑1 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            <  2:2969121/101‑1 (MQ=255)
                                                                     cgggcCGAGGGCACGATCACCGAAGACGCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                            <  1:821481/99‑1 (MQ=255)
                                                                          cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                       >  1:3004982/1‑101 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                      >  2:566982/1‑101 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                      >  2:2190752/1‑101 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                      >  1:1811418/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                    <  1:2721982/101‑1 (MQ=255)
                                                                               gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                  >  2:1573187/1‑101 (MQ=255)
                                                                                cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                 >  2:3444670/1‑101 (MQ=255)
                                                                                cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                 >  2:1584819/1‑101 (MQ=255)
                                                                                 aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtc                >  2:416181/1‑100 (MQ=255)
                                                                                   gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc              >  2:4557895/1‑101 (MQ=255)
                                                                                    aTCACCGAAGACCCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa             <  2:3751451/101‑1 (MQ=255)
                                                                                       aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc          <  1:264998/101‑1 (MQ=255)
                                                                                       aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc          <  1:3781431/101‑1 (MQ=255)
                                                                                        ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc         <  2:2372975/101‑1 (MQ=255)
                                                                                          gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga       <  2:3071962/101‑1 (MQ=255)
                                                                                          gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga       >  2:1623817/1‑101 (MQ=255)
                                                                                          gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga       >  1:4500887/1‑101 (MQ=255)
                                                                                          gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga       >  1:4596556/1‑101 (MQ=255)
                                                                                            aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga     >  2:1372264/1‑101 (MQ=255)
                                                                                            aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga     <  1:1272362/101‑1 (MQ=255)
                                                                                            aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCAGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga     <  1:3317341/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   <  1:1898658/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531506‑3531701

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG