Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,653 T→C 100% F427L (TTC→CTC)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6530T→C100.0% 812.6 218F427L (TTC→CTC) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (108/108):  ref base (0/0):  total (109/109)

Only 100 of 228 total aligned reads displayed.

AGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCA  >  NC_014034/3531555‑3531750
                                                                                                  |                                                                                                 
 gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                                                                                >  1:1075787/1‑101 (MQ=255)
 gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                                                                                <  2:1452310/101‑1 (MQ=255)
  cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                                                                               >  1:675976/1‑101 (MQ=255)
  cAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCgg                                                                                               <  1:2325212/101‑1 (MQ=255)
   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                                                                              <  2:1098642/101‑1 (MQ=255)
   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                                                                              >  1:99891/1‑101 (MQ=255)
   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                                                                              <  1:1641278/101‑1 (MQ=255)
     gTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACCCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcg                                                                                            <  2:326308/101‑1 (MQ=255)
       gcgcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgcg                                                                                          >  2:1970514/1‑101 (MQ=255)
          cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                                                                       <  2:138789/101‑1 (MQ=255)
          cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                                                                       >  1:1834930/1‑101 (MQ=255)
            ggcAGTGGCTGGCCGAGGGCCCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGcc                                                                                     <  2:4158780/100‑1 (MQ=255)
             gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACGCGCCCCTCGGCCGCGAAGCCg                                                                                    >  1:2030187/1‑101 (MQ=255)
              cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                                                                   >  2:4407000/1‑101 (MQ=255)
              cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                                                                   <  1:119029/101‑1 (MQ=255)
                gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                                                                 <  1:2570913/101‑1 (MQ=255)
                   gctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                                                                              <  1:4063928/101‑3 (MQ=255)
                    gtggcCGAGGGCACGATCGCGGAAGACGCCTATCCGAAGGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                             <  2:1673379/100‑1 (MQ=255)
                     tggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                            <  1:489638/101‑1 (MQ=255)
                     tggcCGAGGCCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                            <  2:552538/101‑1 (MQ=255)
                      ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                           >  1:856932/1‑101 (MQ=255)
                       gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCATCGGCCGCGAAGCCGATGCCTGGCt                                                                          <  1:2974378/101‑1 (MQ=255)
                         cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                        >  1:3004982/1‑101 (MQ=255)
                         cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                        >  2:1598607/1‑101 (MQ=255)
                         cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                        <  2:1067312/101‑1 (MQ=255)
                           aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                      <  1:513216/101‑1 (MQ=255)
                           aGGCCACGATCACCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                      <  2:3116666/101‑1 (MQ=255)
                            gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                                                                     >  2:1405918/1‑101 (MQ=255)
                              gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                                                                   >  2:1573187/1‑101 (MQ=255)
                               cACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCt                                                                  >  2:3444670/1‑101 (MQ=255)
                                ccGATCACCGAAGACGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                 <  1:403565/100‑1 (MQ=255)
                                aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                 <  2:4619924/101‑1 (MQ=255)
                                  gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                               >  2:4557895/1‑101 (MQ=255)
                                   aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                              >  2:4172411/1‑101 (MQ=255)
                                   aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                              <  2:3486269/101‑1 (MQ=255)
                                    tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                             <  1:4084728/101‑1 (MQ=255)
                                    ccACGGAGGACGCCTACCCGAACGGGCCGGAGCTGATGAAGCCCGACTATGACGACCCGCCCCAGGGCCGCGAAGCCGATGCCTGGCTCAAGGGCTTGCAg                                                             <  2:3580794/100‑1 (MQ=255)
                                     cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa                                                            >  1:132838/1‑101 (MQ=255)
                                     cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa                                                            >  2:4527103/1‑101 (MQ=255)
                                     cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGACCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa                                                            <  1:4645285/101‑1 (MQ=255)
                                      aCCGAAGACGCCTAGCGGACCGGGCCCGAGCTGATTAAGCCCTACTATGACGACTCGCCCCTCGGCCGGGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                           <  1:222317/101‑1 (MQ=255)
                                         gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCACGGCCTTCCAGACCga                                                        >  2:571998/1‑101 (MQ=255)
                                         gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                        >  1:3733675/1‑101 (MQ=255)
                                         gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                        >  1:4500887/1‑101 (MQ=255)
                                          aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                       <  2:3375798/101‑1 (MQ=255)
                                           aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                      <  1:1272362/101‑1 (MQ=255)
                                           aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                      <  1:611170/101‑1 (MQ=255)
                                             aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                    >  1:411851/1‑101 (MQ=255)
                                             aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                    <  1:1898658/101‑1 (MQ=255)
                                              cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                   >  1:1130286/1‑101 (MQ=255)
                                                 ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                <  1:175380/100‑1 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                               >  1:4206027/1‑101 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                               >  2:4619902/1‑101 (MQ=255)
                                                   aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                              >  1:1963850/1‑101 (MQ=255)
                                                    tCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgc                                             >  2:3058188/1‑101 (MQ=255)
                                                      cGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCCACgcgg                                           >  1:1369019/1‑101 (MQ=255)
                                                        aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCGTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                         >  2:2383030/1‑101 (MQ=255)
                                                         aCGGGCCCGAGCTGATGAAGCCGGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                        <  1:3891742/101‑1 (MQ=255)
                                                         aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                        <  2:2845667/101‑1 (MQ=255)
                                                          cGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcgg                                       <  2:785978/101‑1 (MQ=255)
                                                           gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                      <  2:2376674/101‑1 (MQ=255)
                                                             gCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCggggg                                    >  1:1414602/1‑101 (MQ=255)
                                                               ccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtg                                  >  1:793121/1‑101 (MQ=255)
                                                                cGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                 >  1:286065/1‑101 (MQ=255)
                                                                  aGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGTGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTc                               >  2:234617/1‑101 (MQ=255)
                                                                   gCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGGCCGAGATCGCGACGCGGGCGGGGGTGTTcc                              <  2:1929841/101‑1 (MQ=255)
                                                                    cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                             <  1:2595244/101‑1 (MQ=255)
                                                                    cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                             <  2:87128/101‑1 (MQ=255)
                                                                    cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCca                             >  1:2142928/1‑101 (MQ=255)
                                                                     tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCGGATGCCTGGCTCAAGGCCTTCTAGACCGCTTTCGCGACGCTGGCGGGGGTATTCcac                            >  1:762440/1‑101 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                           <  2:2507256/101‑1 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                           >  2:2621836/1‑101 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                           <  2:4233538/101‑1 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCCAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATGGCGACGCGGGCGGGGGTGTTCcaca                           >  1:4575168/1‑101 (MQ=255)
                                                                        tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACaag                         >  1:2382840/1‑100 (MQ=255)
                                                                        tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAAc                         >  1:3520218/1‑101 (MQ=255)
                                                                         gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAcc                        <  1:4244115/101‑1 (MQ=255)
                                                                          aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                       >  1:3467446/1‑101 (MQ=255)
                                                                          aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                       <  1:3393487/101‑1 (MQ=255)
                                                                            gCCCGACTATGACGACACGCCCCTCGCCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGa                     <  1:2804777/101‑1 (MQ=255)
                                                                             cccGCCTATGCCGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGAt                    <  2:1800776/101‑1 (MQ=255)
                                                                                gACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                 <  2:2770521/101‑1 (MQ=255)
                                                                                gACTATGACGACACCCCCCCCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                 <  1:955523/101‑1 (MQ=255)
                                                                                  cTATGCCGCCCCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAccc               <  2:3802306/101‑1 (MQ=255)
                                                                                   tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt              >  1:2003717/1‑101 (MQ=255)
                                                                                   tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt              >  1:2695728/1‑101 (MQ=255)
                                                                                    aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg             >  1:1216855/1‑101 (MQ=255)
                                                                                     tGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGc            >  1:3277993/1‑101 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa         <  2:829604/101‑1 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCa         >  1:3610442/1‑101 (MQ=255)
                                                                                         gacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAc        <  2:4483867/101‑1 (MQ=255)
                                                                                         gacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCAc        >  1:1572716/1‑101 (MQ=255)
                                                                                          acacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAcc       <  1:1584924/101‑1 (MQ=255)
                                                                                           cacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCt      <  2:3467192/101‑1 (MQ=255)
                                                                                            acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt     <  1:1469053/101‑1 (MQ=255)
                                                                                              gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc   >  1:3547329/1‑101 (MQ=255)
                                                                                              ccccCTCGGCCGCGAAGCCGATGCCTGGCTCAAAGCCATCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc   <  1:3984557/100‑1 (MQ=255)
                                                                                               cccCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCa  <  2:2952482/101‑1 (MQ=255)
                                                                                               cccCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCa  <  2:2055179/101‑1 (MQ=255)
                                                                                               cccCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGAGTTCCACAACCTGATCACCCTGCCCACCTTCCa  >  2:3984272/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCA  >  NC_014034/3531555‑3531750

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG