Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,673 G→C 100% A433A (GCG→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6730G→C100.0% 899.2 235A433A (GCG→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (112/123):  ref base (0/0):  total (112/123)

Only 100 of 241 total aligned reads displayed.

CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770
                                                                                                  |                                                                                                 
ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTg                                                                                                 <  2:2969121/101‑1 (MQ=255)
 tggcCGAGGCCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTgg                                                                                                <  2:552538/101‑1 (MQ=255)
  ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                                               >  1:213118/1‑101 (MQ=255)
  ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                                               >  1:856932/1‑101 (MQ=255)
   gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCATCGGCCGCGAAGCCGATGCCTGGCt                                                                                              <  1:2974378/101‑1 (MQ=255)
     cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            >  2:1598607/1‑101 (MQ=255)
     cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            <  2:1067312/101‑1 (MQ=255)
     cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            <  2:3440325/101‑1 (MQ=255)
     cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            >  1:3004982/1‑101 (MQ=255)
     cGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCa                                                                                            >  1:2731592/1‑101 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  1:1419763/1‑101 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  1:1811418/1‑101 (MQ=255)
       aGGCCACGATCACCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                                          <  2:3116666/101‑1 (MQ=255)
            ccGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                                     <  2:484209/100‑1 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   >  2:4557895/1‑101 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   >  1:3206083/1‑101 (MQ=255)
              gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  2:4103750/101‑1 (MQ=255)
               aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                                                  >  1:239223/1‑101 (MQ=255)
               aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                                                  <  2:3486269/101‑1 (MQ=255)
               aTCACCGAAGACCCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                                                  <  2:3751451/101‑1 (MQ=255)
                tCACCGAAGCCGCCTATCCGAACGGGCCGGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  2:672795/101‑1 (MQ=255)
                tCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAg                                                                                 <  1:4084728/101‑1 (MQ=255)
                 cACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGACCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGa                                                                                <  1:4645285/101‑1 (MQ=255)
                  aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                                               <  1:264998/101‑1 (MQ=255)
                  aCCGAAGACGCCTAGCGGACCGGGCCCGAGCTGATTAAGCCCTACTATGACGACTCGCCCCTCGGCCGGGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                                               <  1:222317/101‑1 (MQ=255)
                     gAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCACGGCCTTCCAGACCga                                                                            >  2:571998/1‑101 (MQ=255)
                       aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                                          >  2:1372264/1‑101 (MQ=255)
                       aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                                          <  1:611170/101‑1 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  1:1898658/101‑1 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        >  1:411851/1‑101 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        <  1:455575/101‑1 (MQ=255)
                          cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                                       >  1:1130286/1‑101 (MQ=255)
                              tATCCGAACGGGCCCGAGCTGAGGACGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGACACCTGCCTCAAGGCCTACCAGACCgacgccactag                                                                   >  2:4619908/1‑92 (MQ=255)
                               aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGGCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                                                  >  1:4004204/1‑101 (MQ=255)
                               aTCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcg                                                                  >  1:1963850/1‑101 (MQ=255)
                                 ccGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgcg                                                                <  2:3255634/101‑1 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  2:3369905/1‑101 (MQ=255)
                                     aCGGGCCCGAGCTGATGAAGCCGGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                                            <  1:3891742/101‑1 (MQ=255)
                                     aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                                            <  2:2845667/101‑1 (MQ=255)
                                     aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                                            >  2:3465418/1‑101 (MQ=255)
                                      cGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcgg                                                           <  2:785978/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          <  2:2376674/101‑1 (MQ=255)
                                        ggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                                         >  1:422733/1‑101 (MQ=255)
                                         gCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCggggg                                                        >  1:1282291/1‑101 (MQ=255)
                                         gCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCggggg                                                        >  1:4451493/1‑101 (MQ=255)
                                          cccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggggg                                                       >  2:4205353/1‑100 (MQ=255)
                                          cccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       <  2:4596257/101‑1 (MQ=255)
                                            cGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                                     >  1:286065/1‑101 (MQ=255)
                                             gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                                    <  1:1406013/101‑1 (MQ=255)
                                             gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                                                    >  1:1260349/1‑101 (MQ=255)
                                              aGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGTGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTc                                                   >  2:234617/1‑101 (MQ=255)
                                               gCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTcc                                                  <  1:507299/101‑1 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 >  1:1200933/1‑101 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCca                                                 >  1:2142928/1‑101 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCGGATGCCTGGCTCAAGGCCTTCTAGACCGCTTTCGCGACGCTGGCGGGGGTATTCcac                                                >  1:762440/1‑101 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               <  2:4233538/101‑1 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               <  2:2507256/101‑1 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                                               >  2:2621836/1‑101 (MQ=255)
                                                  gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGCGTTCcaca                                               >  2:3553530/1‑101 (MQ=255)
                                                    tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                                             >  1:2579685/1‑101 (MQ=255)
                                                    tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                                             >  2:1747987/1‑101 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           <  1:3393487/101‑1 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           >  1:3467446/1‑101 (MQ=255)
                                                          ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                                       <  1:289043/101‑1 (MQ=255)
                                                          ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                                       <  2:440856/101‑1 (MQ=255)
                                                           cGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATcn                                      <  1:158313/101‑2 (MQ=255)
                                                            gACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                                     <  1:161290/101‑1 (MQ=255)
                                                            gACTATGACGACACCCCCCCCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                                     <  1:955523/101‑1 (MQ=255)
                                                               tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt                                  >  1:2695728/1‑101 (MQ=255)
                                                               tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt                                  >  1:2003717/1‑101 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             <  1:3532212/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             <  2:829604/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCa                             >  1:3610442/1‑101 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  2:1811292/101‑1 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  1:1469053/101‑1 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         >  1:674513/1‑101 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCACCtt                         >  2:4518713/1‑101 (MQ=255)
                                                                          gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc                       <  2:1557954/101‑1 (MQ=255)
                                                                          ccccCTCGGCCGCGAAGCCGATGCCTGGCTCAAAGCCATCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc                       <  1:3984557/100‑1 (MQ=255)
                                                                             cctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATc                    <  1:1740163/99‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  2:2129757/1‑101 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                <  2:1579106/101‑1 (MQ=255)
                                                                                  gCCGCGAAGCCGATGCCTTGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAcc               <  1:3823183/101‑1 (MQ=255)
                                                                                  gCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAcc               <  2:1232041/101‑1 (MQ=255)
                                                                                    cgcgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACcgc             <  1:2279542/101‑1 (MQ=255)
                                                                                      cgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGa           >  1:4575654/1‑101 (MQ=255)
                                                                                      cgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTAATCACCCTGCCCACCTTCCATCTGACcgcta           >  1:1095442/1‑99 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  2:3968875/1‑101 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  2:1403066/1‑101 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          >  1:3738642/1‑101 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         <  1:4519008/101‑1 (MQ=255)
                                                                                         aGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAAt        <  2:4298765/101‑1 (MQ=255)
                                                                                         aGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAAt        >  1:4238931/1‑101 (MQ=255)
                                                                                         aGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAAt        <  1:3553788/101‑1 (MQ=255)
                                                                                           ccGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCg      <  2:1942545/101‑1 (MQ=255)
                                                                                            cGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCga     >  2:2425314/1‑101 (MQ=255)
                                                                                            cGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCga     >  1:91376/1‑101 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   >  2:2195297/1‑101 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   >  1:587229/1‑101 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   >  1:2097692/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG