Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,539,498 T→G 100% D261E (GAT→GAG prpD → 2‑methylcitrate dehydratase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,539,4980T→G100.0% 796.7 217D261E (GAT→GAGprpD2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  new base (92/124):  ref base (0/0):  total (93/124)

Only 100 of 244 total aligned reads displayed.

ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595
                                                                                                  |                                                                                                 
aTCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGGCGACGGTTTCCTCGCCTGGACGCTCTCGGGCCCAGAGgc                                                                                                 >  2:4203355/1‑101 (MQ=255)
aTCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGgc                                                                                                 <  2:451829/101‑1 (MQ=255)
       cGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTac                                                                                          >  2:1837328/1‑101 (MQ=255)
       cGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTac                                                                                          >  1:4584697/1‑101 (MQ=255)
             aCCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTc                                                                                    >  1:2505138/1‑101 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   >  2:2467943/1‑101 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:1314899/101‑1 (MQ=255)
               cgcgCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCg                                                                                  <  1:166501/101‑1 (MQ=255)
                 cgcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGcc                                                                                <  1:903327/101‑1 (MQ=255)
                  gcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCt                                                                               >  1:3273813/1‑101 (MQ=255)
                    ccTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGc                                                                             >  1:1025479/1‑101 (MQ=255)
                    ccTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGc                                                                             <  2:1191553/101‑1 (MQ=255)
                      tGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGccc                                                                           <  2:1427527/101‑1 (MQ=255)
                             cgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATg                                                                    <  1:436120/101‑1 (MQ=255)
                             cgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATg                                                                    >  1:2609614/1‑101 (MQ=255)
                             cgAAGGCCCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATg                                                                    <  1:1002611/101‑1 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  1:4475281/101‑1 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  >  1:3293472/1‑101 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  <  2:461110/101‑1 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 >  1:3896894/1‑101 (MQ=255)
                                 ggCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGa                                                                <  1:460567/101‑1 (MQ=255)
                                 ggCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGa                                                                <  1:3044925/101‑1 (MQ=255)
                                   cgcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGc                                                              <  1:116938/101‑1 (MQ=255)
                                   cgcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGc                                                              <  1:2033466/101‑1 (MQ=255)
                                     cccccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCg                                                            <  2:4293141/101‑1 (MQ=255)
                                       cccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaa                                                          <  2:2323754/101‑1 (MQ=255)
                                        ccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaaa                                                         >  1:3980801/1‑101 (MQ=255)
                                         cAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        <  2:1753762/101‑1 (MQ=255)
                                          aGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  2:3496880/1‑101 (MQ=255)
                                          aGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       <  1:2114341/101‑1 (MQ=255)
                                             cccGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACgcgc                                                    >  1:3653703/1‑101 (MQ=255)
                                             cccGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACgcgc                                                    >  2:3315117/1‑101 (MQ=255)
                                               cGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCa                                                  <  2:4428213/101‑1 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCGTCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  1:2649004/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  1:2254288/1‑101 (MQ=255)
                                                   tGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCAGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCt                                              >  1:3935315/1‑101 (MQ=255)
                                                    gggAGGGGGAAGAGGGTTTCATCGCCTGGATGCTCTCGGGCCCGGAGGGGCGTTACGCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  1:2379386/101‑1 (MQ=255)
                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCGCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            <  1:3513800/101‑1 (MQ=255)
                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            <  1:1572208/101‑1 (MQ=255)
                                                     ggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAAGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTg                                            >  1:1132701/1‑101 (MQ=255)
                                                       ggggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGac                                          <  2:3616453/100‑1 (MQ=255)
                                                           ggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCt                                      >  1:501469/1‑101 (MQ=255)
                                                           ggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCt                                      >  2:1739122/1‑101 (MQ=255)
                                                           ggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCt                                      <  1:4224449/101‑1 (MQ=255)
                                                           ggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGCCCATGGCGAGCCGAAACGCGCCATCCTTGACACCt                                      >  1:293981/1‑101 (MQ=255)
                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     >  2:875196/1‑101 (MQ=255)
                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCTTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  2:1076430/101‑1 (MQ=255)
                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  2:4130144/101‑1 (MQ=255)
                                                               gACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAc                                  <  1:347989/101‑1 (MQ=255)
                                                               gACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAc                                  <  1:67923/101‑1 (MQ=255)
                                                                aCGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAcc                                 <  2:3674138/101‑1 (MQ=255)
                                                                aCGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAcc                                 <  2:3924727/101‑1 (MQ=255)
                                                                aCGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATAcc                                 <  1:1415537/101‑1 (MQ=255)
                                                                  ggTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCaa                               >  1:2799795/1‑101 (MQ=255)
                                                                  ggTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCaa                               <  1:2457528/101‑1 (MQ=255)
                                                                   gTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAg                              <  2:477953/101‑1 (MQ=255)
                                                                     ttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGa                            >  2:2749984/1‑101 (MQ=255)
                                                                      tCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGaa                           <  1:2222335/101‑1 (MQ=255)
                                                                        aTCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAata                         >  1:4214830/1‑101 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  2:4629400/101‑1 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        >  2:2280856/1‑101 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  1:1158465/101‑1 (MQ=255)
                                                                         tCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  1:1837454/101‑1 (MQ=255)
                                                                          cGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATAtt                       <  2:2010681/101‑1 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      >  1:271136/1‑101 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      <  2:2232419/101‑1 (MQ=255)
                                                                           gCCTGGAGGCCCCCGGGCCAAGAGGCGCGTTGCCCCGGCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      <  1:588230/101‑1 (MQ=255)
                                                                            ccTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGGAACGCGCCATCCTTGACACCTATACCAAGGAATATTcg                     >  1:1752199/1‑101 (MQ=255)
                                                                            ccTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcg                     <  2:1508405/101‑1 (MQ=255)
                                                                             cTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGTCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcgg                    <  2:2382780/101‑1 (MQ=255)
                                                                              tGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   <  2:956105/101‑1 (MQ=255)
                                                                              tGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   >  2:582846/1‑101 (MQ=255)
                                                                              tGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggc                   >  2:2213006/1‑101 (MQ=255)
                                                                                gATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcgg                 >  2:3064823/1‑101 (MQ=255)
                                                                                gATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcgg                 >  1:2192081/1‑101 (MQ=255)
                                                                                gATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcggcgg                 <  2:3180421/101‑1 (MQ=255)
                                                                                 aTGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGa                >  2:3239480/1‑101 (MQ=255)
                                                                                  tGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCAATCCTTGACACCTATACCAAGGAATATTCGACGGac               >  2:3014405/1‑100 (MQ=255)
                                                                                   gCTATCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAat              >  2:3954899/1‑101 (MQ=255)
                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGTCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             >  2:1150287/1‑101 (MQ=255)
                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             <  1:377642/101‑1 (MQ=255)
                                                                                    ctctCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAata             <  1:4398130/101‑1 (MQ=255)
                                                                                     tctcGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  2:2648819/101‑1 (MQ=255)
                                                                                     tctcGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  2:1010036/101‑1 (MQ=255)
                                                                                     tctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGACAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            >  2:4031854/1‑101 (MQ=255)
                                                                                      ctcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           >  2:2110481/1‑101 (MQ=255)
                                                                                      cccGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATc           <  1:3257965/99‑1 (MQ=255)
                                                                                       tcGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCa          >  2:4115677/1‑101 (MQ=255)
                                                                                       ccGGGCCCGGGGGCGTGTTACACCGCCCGCCTGCCCGGCCAGGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCa          <  1:490045/100‑1 (MQ=255)
                                                                                       ccGGGCCCAGAGGCGCGTTACACCGCCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCa          <  2:2076233/100‑1 (MQ=255)
                                                                                        cGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  1:1855058/101‑1 (MQ=255)
                                                                                        cGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         >  1:3233636/1‑101 (MQ=255)
                                                                                         gggCCAAGAGGCGTTTGACACCTTCCCCCTTCCCGGCCATGGCGAGCCGAAACGCTCCATCCTTGACCCCTATACCAAGGAATATTCGGCGGGATATCaaa        <  1:3014634/101‑1 (MQ=255)
                                                                                          ggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAg       <  2:807269/101‑1 (MQ=255)
                                                                                           gCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCCCCATCCTTGACACCTATACCAACGAATATTCGCCGGAATATCAAAGc      >  2:558197/1‑101 (MQ=255)
                                                                                            cccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGcc     >  2:2813759/1‑101 (MQ=255)
                                                                                              cAGAGGCGCGTTCCCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   <  2:3073240/101‑1 (MQ=255)
                                                                                              cAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   <  1:3071260/101‑1 (MQ=255)
                                                                                              cAGAGGCGCGTTACACCGTACGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAg   >  2:2860239/1‑101 (MQ=255)
                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  >  2:702498/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG