Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,541,575 G→C 100% S53S (TCG→TCC prpB → methylisocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,541,5750G→C100.0% 888.2 231S53S (TCG→TCCprpBmethylisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (113/118):  ref base (0/0):  total (113/118)

Only 100 of 237 total aligned reads displayed.

CCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541478‑3541672
                                                                                                 |                                                                                                 
cccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgc                                                                                                <  1:1796884/101‑1 (MQ=255)
cccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgc                                                                                                <  1:1685394/101‑1 (MQ=255)
 ccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGgcg                                                                                               >  1:1079183/1‑101 (MQ=255)
   tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             <  2:4214721/101‑1 (MQ=255)
    ggcccggcccGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcgg                                                                                            >  2:2321654/1‑101 (MQ=255)
      cccggcccggCATCCTGCGCCTGCCGGGCGCGCATAACGGCACGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcggcg                                                                                          <  2:3204567/101‑1 (MQ=255)
        cggcccggcGTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                        <  1:1945389/101‑1 (MQ=255)
        cggcccggcATCCTGCGCCTGCCGGGNNCNNATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                        <  1:2957259/101‑1 (MQ=255)
         ggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATg                                                                                       <  2:1144347/101‑1 (MQ=255)
          nnnnnnnaTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGt                                                                                      <  1:1513369/94‑1 (MQ=255)
          gcccggcATCCTGCTCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGt                                                                                      <  2:479611/101‑1 (MQ=255)
          gcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGt                                                                                      <  2:4579742/101‑1 (MQ=255)
           cccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTc                                                                                     >  1:4186746/1‑101 (MQ=255)
            ccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCg                                                                                    >  2:3727425/1‑101 (MQ=255)
              ggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGc                                                                                  >  2:4326490/1‑101 (MQ=255)
              ggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGc                                                                                  >  1:3187776/1‑101 (MQ=255)
                cATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCt                                                                                <  2:4182192/101‑1 (MQ=255)
                 aTCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGGGCGGCGATGTCGGCCTc                                                                               >  1:51624/1‑101 (MQ=255)
                  tCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCg                                                                              >  2:2424237/1‑101 (MQ=255)
                   ccTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTTGGCCTCGa                                                                             >  1:1402250/1‑101 (MQ=255)
                   ccTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGa                                                                             >  2:2343349/1‑101 (MQ=255)
                    cTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGAt                                                                            <  1:3124051/101‑1 (MQ=255)
                     tGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATg                                                                           >  1:4097113/1‑101 (MQ=255)
                      gcgcCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATgg                                                                          <  2:2570577/101‑1 (MQ=255)
                      gcgcCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATgg                                                                          >  2:2854817/1‑101 (MQ=255)
                         ccTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGcc                                                                       <  2:293188/101‑1 (MQ=255)
                         ccTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGcc                                                                       >  2:4574213/1‑101 (MQ=255)
                             ccGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGcc                                                                   <  1:1110240/101‑1 (MQ=255)
                              cGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCg                                                                  >  2:3409061/1‑101 (MQ=255)
                                ggCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGa                                                                >  1:1215610/1‑101 (MQ=255)
                                 gcgcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAc                                                               >  2:4292711/1‑101 (MQ=255)
                                    cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            >  2:366765/1‑101 (MQ=255)
                                    cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            <  2:678123/101‑1 (MQ=255)
                                     gcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCg                                                           >  2:1344970/1‑101 (MQ=255)
                                       aTAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGTTGGGCCTGCCGGACCTCGGc                                                         >  1:4630745/1‑101 (MQ=255)
                                       aTAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGc                                                         >  1:1901103/1‑101 (MQ=255)
                                         aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       <  1:2837349/101‑1 (MQ=255)
                                          aCGGCATGGCCGCTCTGCAGGCGAAAGCCGCGGGATTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      <  1:4008417/101‑1 (MQ=255)
                                          aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      >  1:2215855/1‑101 (MQ=255)
                                           cGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATcc                                                     >  1:261778/1‑101 (MQ=255)
                                            ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGCCCTCGGCATCCt                                                    >  1:1324601/1‑101 (MQ=255)
                                            ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                    >  1:4362533/1‑101 (MQ=255)
                                            ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                    >  2:1439637/1‑101 (MQ=255)
                                             gCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTg                                                   <  2:2795654/101‑1 (MQ=255)
                                              cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                  <  1:1598125/101‑1 (MQ=255)
                                                tGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACg                                                <  1:1839142/101‑1 (MQ=255)
                                                 ggCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACgg                                               >  2:2240454/1‑101 (MQ=255)
                                                  gCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACggg                                              >  2:1995440/1‑100 (MQ=255)
                                                  gCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGt                                              <  2:3531404/101‑1 (MQ=255)
                                                     gcgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGa                                           <  2:1417853/101‑1 (MQ=255)
                                                         tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                       <  1:3907163/101‑1 (MQ=255)
                                                         tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                       <  2:1600158/101‑1 (MQ=255)
                                                            aGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  1:2497111/101‑1 (MQ=255)
                                                                  aaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttt                              >  1:2165854/1‑101 (MQ=255)
                                                                   aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                             >  2:371769/1‑101 (MQ=255)
                                                                     ccGCGGGGTTTCAGGGCCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTc                           <  2:725000/101‑1 (MQ=255)
                                                                     ccGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTc                           >  1:147180/1‑101 (MQ=255)
                                                                      cgcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCa                          <  2:1900968/101‑1 (MQ=255)
                                                                      cgcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCa                          <  2:452742/101‑1 (MQ=255)
                                                                      cgcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCa                          >  2:3613383/1‑101 (MQ=255)
                                                                       gcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCAt                         >  2:3301103/1‑101 (MQ=255)
                                                                       gcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCAt                         <  1:984557/101‑1 (MQ=255)
                                                                       gcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCAt                         >  2:164436/1‑101 (MQ=255)
                                                                        cgGGGATTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGGTGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGTGGTCGCCTTTTTCATc                        >  2:219071/1‑101 (MQ=255)
                                                                         ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       <  1:3740360/101‑1 (MQ=255)
                                                                         ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       <  1:1966815/101‑1 (MQ=255)
                                                                         ggggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       <  2:3282971/101‑1 (MQ=255)
                                                                          gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATccc                      >  2:621194/1‑100 (MQ=255)
                                                                            gtgtCAGGCGCTTTATGTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCCGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGcc                    <  2:4028877/98‑1 (MQ=255)
                                                                             tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                   <  1:1735558/101‑1 (MQ=255)
                                                                             tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                   <  2:1485705/101‑1 (MQ=255)
                                                                             tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                   >  1:2082894/1‑101 (MQ=255)
                                                                              ttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  >  1:3606199/1‑101 (MQ=255)
                                                                              ttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  <  1:2424401/101‑1 (MQ=255)
                                                                              ttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  >  1:4572551/1‑101 (MQ=255)
                                                                                cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt                >  1:1638695/1‑101 (MQ=255)
                                                                                cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt                <  2:3957510/101‑1 (MQ=255)
                                                                                cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt                <  1:607828/101‑1 (MQ=255)
                                                                                cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGAt                <  1:3051472/101‑1 (MQ=255)
                                                                                 aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               >  2:2629625/1‑101 (MQ=255)
                                                                                 aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               >  1:3771292/1‑101 (MQ=255)
                                                                                 aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               >  2:3468932/1‑101 (MQ=255)
                                                                                  ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa              <  2:60737/101‑1 (MQ=255)
                                                                                  ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa              <  1:4480743/101‑1 (MQ=255)
                                                                                     gcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAcgc           <  2:2659746/101‑1 (MQ=255)
                                                                                       tttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCgg         <  2:1184968/101‑1 (MQ=255)
                                                                                       tttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCgg         >  1:1148571/1‑101 (MQ=255)
                                                                                        ttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCggg        >  2:54903/1‑101 (MQ=255)
                                                                                        ttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCggg        >  2:1383682/1‑101 (MQ=255)
                                                                                         tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       <  1:2583691/101‑1 (MQ=255)
                                                                                         tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       <  1:3080602/101‑1 (MQ=255)
                                                                                         tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       >  1:230848/1‑101 (MQ=255)
                                                                                          aTCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGcc      <  1:1995580/101‑1 (MQ=255)
                                                                                            cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc    >  2:3090632/1‑101 (MQ=255)
                                                                                             tGTCCGGCGCGGGGATGTCGGCCTCGATGGGCCTGCCCGGCCTCCGGATCCTGACGGGGGGCGAGGTCGCCTTTTTCATCCCCCCGATCACCCGGGCCCCg   >  2:3588332/1‑101 (MQ=255)
                                                                                             tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   <  2:4519179/101‑1 (MQ=255)
                                                                                             tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   >  2:3875890/1‑101 (MQ=255)
                                                                                             tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   <  2:3764746/101‑1 (MQ=255)
                                                                                             tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   <  2:1215518/101‑1 (MQ=255)
                                                                                              gTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCgg  <  1:3438379/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
CCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541478‑3541672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG