Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 367,286 G→A 100% A214T (GCC→ACC)  bztD → glutamate/aspartate ABC transporter ATP‑binding protein BztD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034367,2860G→A100.0% 1032.9 283A214T (GCC→ACC) bztDglutamate/aspartate ABC transporter ATP‑binding protein BztD
Reads supporting (aligned to +/- strand):  new base (128/148):  ref base (0/3):  total (129/154)

Only 100 of 289 total aligned reads displayed.

TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCC  >  NC_014034/367188‑367382
                                                                                                  |                                                                                                 
tCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAcc                                                                                                 <  1:1601110/101‑1 (MQ=255)
 cgacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAccc                                                                                                >  1:1388782/1‑101 (MQ=255)
   acgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCAt                                                                                              <  1:639028/101‑1 (MQ=255)
    cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                             >  1:147581/1‑101 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                            <  1:1302705/101‑1 (MQ=255)
       aCCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga.ga                                                                                         <  1:496977/101‑1 (MQ=255)
        cccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GAt                                                                                        <  2:901286/101‑1 (MQ=255)
        cccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GAt                                                                                        <  2:4597324/101‑1 (MQ=255)
         ccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATg                                                                                       <  2:3064414/101‑1 (MQ=255)
           aCCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATggg                                                                                     >  1:1726874/1‑101 (MQ=255)
            ccTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGc                                                                                    <  1:1414959/101‑1 (MQ=255)
            ccTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGc                                                                                    <  1:2330977/101‑1 (MQ=255)
             cTCGGCGCTTGGCCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCt                                                                                   <  1:3414389/101‑1 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCtt                                                                                  <  1:874447/101‑1 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCtt                                                                                  <  1:691301/101‑1 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCtt                                                                                  <  1:2462271/101‑1 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCtt                                                                                  >  2:3304966/1‑101 (MQ=255)
               cGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCttt                                                                                 <  2:4264285/101‑1 (MQ=255)
                  cgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGcc                                                                              >  1:4241878/1‑101 (MQ=255)
                  cgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGcc                                                                              >  2:3493869/1‑101 (MQ=255)
                  cgcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.AATGGGCTTTGcc                                                                              >  2:134598/1‑101 (MQ=255)
                   gcTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGccc                                                                             <  1:2148988/101‑1 (MQ=255)
                    cTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCa                                                                            <  2:2931026/101‑1 (MQ=255)
                    cTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTCTGCCCa                                                                            >  2:674120/1‑101 (MQ=255)
                     ttGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAg                                                                           <  1:3716952/101‑1 (MQ=255)
                       gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGc                                                                         <  1:394468/101‑1 (MQ=255)
                       gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGCCTTTGCCCAggg                                                                         >  2:3619603/1‑100 (MQ=255)
                       gACCCCGAGATGATCAAGGAAATGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGc                                                                         >  1:3439833/1‑101 (MQ=255)
                        aCCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCg                                                                        >  1:2989104/1‑101 (MQ=255)
                        aCCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCg                                                                        >  1:4069707/1‑101 (MQ=255)
                         ccccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCgg                                                                       >  2:1672986/1‑101 (MQ=255)
                           ccGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTg                                                                     <  2:2575556/101‑1 (MQ=255)
                             gagaTGATCAAGGAAGTGGTGGATACGATGATCCAGCGCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGc                                                                   <  1:134612/101‑1 (MQ=255)
                             gagaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGc                                                                   >  2:2610176/1‑101 (MQ=255)
                             gagaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGc                                                                   >  2:4538814/1‑101 (MQ=255)
                             gagaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GACGGGCTTTGCCCAGGCGGTGGc                                                                   <  1:2641993/101‑1 (MQ=255)
                              agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCg                                                                  <  2:1212911/101‑1 (MQ=255)
                              agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCg                                                                  <  1:2486705/101‑1 (MQ=255)
                               gatgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGa                                                                 >  2:2451484/1‑101 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGaa                                                                <  2:1873747/101‑1 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGaa                                                                <  2:422666/101‑1 (MQ=255)
                                  gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                              <  1:380001/101‑1 (MQ=255)
                                   atCAAGGAAGTGCTGGATACGATGATCCAGCTCCCCGGGGAAGG.ATGACCATTCCCCGCCGCACCCATGAGGAGGGCCTTTGCCAAGCCGGGGGC.AACCgg                                                            >  2:568863/1‑101 (MQ=255)
                                     cAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCggg                                                           >  2:310935/1‑101 (MQ=255)
                                      aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGt                                                          >  1:4334563/1‑101 (MQ=255)
                                      aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTC.ACCATGA.GATGGGCTTTGCCCAGGGGGTGGCGAACCggggg                                                         >  1:416041/1‑99 (MQ=38)
                                        ggAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGa                                                        <  2:3394244/101‑1 (MQ=255)
                                           aGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCt                                                     <  1:2405144/101‑1 (MQ=255)
                                             tGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTc                                                   <  2:1244250/101‑1 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCAt                                                 <  1:3125125/101‑1 (MQ=255)
                                               cTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGCGATCTTCAt                                                 >  2:439305/1‑101 (MQ=255)
                                                tGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATg                                                <  2:1630889/101‑1 (MQ=255)
                                                 ggATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgg                                               >  1:4080728/1‑101 (MQ=255)
                                                  gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                              <  2:210883/101‑1 (MQ=255)
                                                  gATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGc                                              <  2:3439579/101‑1 (MQ=255)
                                                    tACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCg                                            <  1:4450809/101‑1 (MQ=255)
                                                      cGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGAc                                          <  1:4539109/101‑1 (MQ=255)
                                                       gatgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgagatcgg                                         >  1:1056240/1‑94 (MQ=255)
                                                          gatCCAGATCGCCGAGGAAGGCAGGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCAGAcagaa                                      >  2:3560501/1‑97 (MQ=21)
                                                           atCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCa                                     <  1:2204094/101‑1 (MQ=255)
                                                           atCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCa                                     >  2:2392462/1‑101 (MQ=255)
                                                            tCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAg                                    >  1:3625121/1‑101 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                 <  1:2568985/101‑1 (MQ=255)
                                                                 cTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgt                               <  1:273628/101‑1 (MQ=255)
                                                                  tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                              >  1:1902449/1‑101 (MQ=255)
                                                                  tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                              >  1:2875249/1‑101 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGTGTGATCTTCATGGCCGACGGGCAGAtcgtcg                             <  2:8492/101‑1 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                             >  2:1489934/1‑101 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                             <  2:1991969/101‑1 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                             >  2:2174930/1‑101 (MQ=255)
                                                                    gCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcgg                            >  2:3021276/1‑100 (MQ=255)
                                                                      cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGTTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                          <  2:312668/101‑1 (MQ=255)
                                                                       gAGGAAGGCATGACGATGCTCTGCGTCCCCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                         <  1:2934613/101‑1 (MQ=255)
                                                                       gAGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                         >  2:2065899/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                        >  2:2014297/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                        >  2:803505/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                        >  1:1120763/1‑101 (MQ=255)
                                                                        aGGAAGCCATGACGATCCTCTGCTTC.ACCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGa                       <  2:416004/101‑1 (MQ=37)
                                                                         ggAAGGCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGa                       <  2:408977/101‑1 (MQ=255)
                                                                           aaGGCATGACGATGCTTTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaac                     >  2:4437178/1‑101 (MQ=255)
                                                                              gCATGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaacaac                  >  1:1255836/1‑101 (MQ=255)
                                                                                aTGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                >  1:3471201/1‑101 (MQ=255)
                                                                                aTGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGCCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                >  2:1521136/1‑101 (MQ=255)
                                                                                 tGACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCg               >  1:2447969/1‑101 (MQ=255)
                                                                                  gACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGc              >  2:376446/1‑101 (MQ=255)
                                                                                  gACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGc              >  1:3358611/1‑101 (MQ=255)
                                                                                  gACGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGc              >  2:1154661/1‑101 (MQ=255)
                                                                                    cGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCAt            >  2:2712272/1‑101 (MQ=255)
                                                                                    cGATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCAt            <  1:978983/101‑1 (MQ=255)
                                                                                     gATGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATg           >  1:704505/1‑101 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa          >  1:3669152/1‑101 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa          <  2:2689617/101‑1 (MQ=255)
                                                                                       tGCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAc         <  2:2166703/101‑1 (MQ=255)
                                                                                        gCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGGAGATCGTCGAGCAGAACAACCCGCATGAct        >  2:3861952/1‑101 (MQ=255)
                                                                                        gCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCNNNann        >  1:1455878/1‑99 (MQ=255)
                                                                                        gCTCTGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAct        <  1:1662114/101‑1 (MQ=255)
                                                                                          cctGCGTCACCCATGA.GATGGGATTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtc      <  2:792916/100‑1 (MQ=255)
                                                                                           ctGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtct     <  2:2875033/101‑1 (MQ=255)
                                                                                            tGCGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActtctt    >  2:4040440/1‑101 (MQ=255)
                                                                                              cGTCACCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc  <  1:4304804/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGA.GATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCC  >  NC_014034/367188‑367382

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG