Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,451 A→C 3.8% intergenic (+54/+94) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4510A→C 3.8% 10.2 ‑4.5 ‑0.1186intergenic (+54/+94)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (63/116):  total (69/117)
Fisher's exact test strand distribution p-value = 1.11e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.39e-01

Only 100 of 204 total aligned reads displayed.

GGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCC  >  NC_014034/432352‑432551
                                                                                                     |                                                                                                     
ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGa                                                                                                       <  2:944853/101‑1 (MQ=255)
ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGa                                                                                                       <  2:2568535/101‑1 (MQ=255)
  ggggcgatcCGCGCCCCCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAgg                                                                                                     <  2:3859207/97‑1 (MQ=21)
     tcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCaa                                                                                                  <  1:149656/101‑1 (MQ=255)
      cgatcCGCGCGCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCTAg                                                                                                 <  1:2655095/101‑1 (MQ=25)
      cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAAGCAAg                                                                                                 >  2:1344295/1‑101 (MQ=255)
        atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGT.ATTTGAGGCAAGAt                                                                                               >  2:570830/1‑101 (MQ=255)
        atcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGAt                                                                                               <  1:1047823/101‑1 (MQ=255)
          ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGa                                                                                             <  1:3159152/101‑1 (MQ=255)
          ccGCGCACGCATGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGa                                                                                             <  2:4099460/101‑1 (MQ=21)
            gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGaaa                                                                                           <  2:1274440/101‑1 (MQ=255)
            gcgcACGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGaaa                                                                                           <  1:3907202/101‑1 (MQ=255)
                gcGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                       <  2:4442502/100‑1 (MQ=255)
                aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGT.ATTTTTAGCAAGATGGAAggac                                                                                       >  2:833919/1‑98 (MQ=18)
                aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                       <  2:1598431/101‑1 (MQ=255)
                aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                       <  2:2707178/101‑1 (MQ=255)
                aCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCg                                                                                       <  2:3375059/101‑1 (MQ=255)
                   cTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATc                                                                                    <  2:1974829/101‑1 (MQ=255)
                         ggggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                              <  1:853348/99‑1 (MQ=255)
                         gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCCAGATGAAAGCCGATCCGGGCt                                                                              <  1:3433570/101‑1 (MQ=255)
                           ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                            <  2:4309508/101‑1 (MQ=255)
                            gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                           <  1:3270444/101‑1 (MQ=255)
                             cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                          >  2:4520429/1‑101 (MQ=255)
                             cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTATGGCAAGATGAAAGGCCATCCCGGCTTCGt                                                                          >  1:273292/1‑101 (MQ=14)
                              ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGGAAAATGAAAGACGGTCCggggcttggt                                                                         >  1:38039/1‑94 (MQ=17)
                              ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                         <  2:3735403/101‑1 (MQ=255)
                               gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                        <  1:1638360/101‑1 (MQ=255)
                                cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                       <  2:3050214/101‑1 (MQ=255)
                                cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                       >  2:630798/1‑101 (MQ=255)
                                cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                       <  2:869401/101‑1 (MQ=255)
                                  ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                     <  2:1129175/101‑1 (MQ=255)
                                    ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                  <  1:1954685/101‑1 (MQ=255)
                                    ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                  <  2:1574779/101‑1 (MQ=255)
                                     cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                 <  1:3752183/101‑1 (MQ=255)
                                     cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                 <  1:1474658/101‑1 (MQ=255)
                                      aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTg                                                                <  1:4666643/101‑1 (MQ=255)
                                       cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGc                                                               <  1:4167960/101‑1 (MQ=255)
                                         ccTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGccc                                                             >  2:3957674/1‑101 (MQ=255)
                                            gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                          >  1:161997/1‑101 (MQ=255)
                                             aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTTGCCCCaa                                                          >  2:3207103/1‑101 (MQ=2)
                                             aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                         <  2:1736919/101‑1 (MQ=255)
                                              ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGGTTCGTTTCATC.TTGCCCCAAat                                                        <  2:2939540/101‑1 (MQ=255)
                                               gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                       >  2:616582/1‑101 (MQ=255)
                                               gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                       <  1:4201899/101‑1 (MQ=255)
                                                  gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                    <  2:4264487/101‑1 (MQ=255)
                                                  gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                    <  1:2185617/101‑1 (MQ=255)
                                                  gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                    >  1:3722448/1‑101 (MQ=255)
                                                  gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                    >  1:4312483/1‑101 (MQ=255)
                                                  gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAG.ATTTGAGGGAAGATGAAAGCCGATCAGGGCTTCTTTTCATC.TTCACCCAAAAATcc                                                    >  1:3011440/1‑101 (MQ=18)
                                                   ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAtt                                        >  1:4436668/5‑101 (MQ=2)
                                                   ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATccc                                                   <  2:351802/101‑1 (MQ=255)
                                                    gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                       <  1:4617392/98‑2 (MQ=2)
                                                    gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                       >  1:3063551/4‑100 (MQ=2)
                                                    gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                       >  1:2388947/4‑100 (MQ=2)
                                                    gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCg                                                  <  1:19822/101‑1 (MQ=255)
                                                     acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttgg                                      >  1:2229861/3‑99 (MQ=2)
                                                      cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttggc                                     >  2:4559270/2‑98 (MQ=2)
                                                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAATCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                                                >  2:4638799/1‑101 (MQ=255)
                                                      aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCGTCGTTTCATC.TTGCCCCAAATATCCCggg                                                >  2:2142120/1‑101 (MQ=255)
                                                       ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCgggg                                               >  1:4644551/1‑101 (MQ=255)
                                                          gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                            <  1:503068/101‑1 (MQ=255)
                                                           ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGa                                           <  2:757808/101‑1 (MQ=25)
                                                             cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                         <  2:2824239/101‑1 (MQ=255)
                                                              gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAtt                                        >  1:750196/1‑101 (MQ=255)
                                                              ccTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                                        <  2:2867068/100‑1 (MQ=255)
                                                               cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                       <  2:2282586/101‑1 (MQ=255)
                                                               cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                       >  2:2511118/1‑101 (MQ=255)
                                                                  ccccccGTCCTGCGGACCCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTgg                                    <  2:4189086/101‑1 (MQ=255)
                                                                       cgtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                               >  1:157086/5‑99 (MQ=255)
                                                                        gtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                              <  1:4540420/98‑1 (MQ=255)
                                                                         tccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                             >  2:3334519/3‑101 (MQ=255)
                                                                          ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                            >  1:1863663/2‑101 (MQ=255)
                                                                          ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                            <  1:4351667/100‑1 (MQ=255)
                                                                          ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                            <  1:3360966/100‑1 (MQ=255)
                                                                          ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                            <  2:4066967/100‑1 (MQ=255)
                                                                            tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                          <  2:2013252/101‑1 (MQ=255)
                                                                            tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                          <  2:1628959/101‑1 (MQ=255)
                                                                             gCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                         <  1:4290940/101‑1 (MQ=255)
                                                                               ggACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                       >  1:2011061/1‑101 (MQ=255)
                                                                                gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                      <  1:2740740/101‑1 (MQ=255)
                                                                                gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                      >  1:2293202/1‑101 (MQ=255)
                                                                                gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                      <  2:3018378/101‑1 (MQ=255)
                                                                                    ccccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                  >  2:1787468/1‑101 (MQ=255)
                                                                                     cccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                 <  2:2743097/101‑1 (MQ=255)
                                                                                      ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                >  2:3830828/1‑101 (MQ=255)
                                                                                       cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCTTTTCATA.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGTGGCAg               >  2:1509760/1‑101 (MQ=255)
                                                                                       cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg               >  2:783530/1‑101 (MQ=255)
                                                                                        ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGGTGCGTTTCATC.TTGCCCCAAATGTGCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc              <  1:155314/101‑1 (MQ=25)
                                                                                         cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTGCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg             >  2:2377296/1‑101 (MQ=255)
                                                                                            gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc          >  1:4047097/1‑101 (MQ=255)
                                                                                            gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc          <  1:4382486/101‑1 (MQ=255)
                                                                                             at.atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc         <  1:2973486/101‑1 (MQ=255)
                                                                                              t.atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc        >  1:2873087/1‑101 (MQ=255)
                                                                                                atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt       <  1:2813349/101‑1 (MQ=255)
                                                                                                atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt       <  1:1769233/101‑1 (MQ=255)
                                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa      >  1:1121381/1‑101 (MQ=255)
                                                                                                 tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa      >  1:1184163/1‑101 (MQ=255)
                                                                                                  ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt     >  2:168727/1‑101 (MQ=255)
                                                                                                     aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc  >  1:2836223/1‑101 (MQ=255)
                                                                                                     aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc  >  2:3631547/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCC  >  NC_014034/432352‑432551

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG