Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,476 T→A 3.8% intergenic (+79/+69) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4760T→A 3.8% 11.9 ‑2.8 ‑0.2186intergenic (+79/+69)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (84/94):  total (91/95)
Fisher's exact test strand distribution p-value = 5.92e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 6.82e-01

Only 100 of 206 total aligned reads displayed.

GCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCC  >  NC_014034/432377‑432576
                                                                                                     |                                                                                                     
ggggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                                       <  1:853348/99‑1 (MQ=255)
gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCt                                                                                                       >  1:1804970/1‑101 (MQ=255)
 cggcgggggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                                      <  1:2845501/101‑1 (MQ=255)
 cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                                      >  2:250936/1‑101 (MQ=255)
  ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                                     <  2:4309508/101‑1 (MQ=255)
   gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                                    <  2:2373731/101‑1 (MQ=255)
   gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                                    <  1:3270444/101‑1 (MQ=255)
    cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                                   >  2:2929000/1‑101 (MQ=255)
    cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                                   >  2:4520429/1‑101 (MQ=255)
    cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGt                                                                                                   <  1:3670427/101‑1 (MQ=255)
    cggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTATGGCAAGATGAAAGGCCATCCCGGCTTCGt                                                                                                   >  1:273292/1‑101 (MQ=14)
      gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                                 <  1:1638360/101‑1 (MQ=255)
       cggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                                <  2:3050214/101‑1 (MQ=255)
         ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                              <  2:1129175/101‑1 (MQ=255)
           ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                                                                           <  1:1954685/101‑1 (MQ=255)
            cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                                          <  1:3752183/101‑1 (MQ=255)
            cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                                          <  1:1474658/101‑1 (MQ=255)
             aCGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTg                                                                                         <  1:4666643/101‑1 (MQ=255)
              cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGc                                                                                        <  1:4167960/101‑1 (MQ=255)
                ccTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGccc                                                                                      >  2:3957674/1‑101 (MQ=255)
                  tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                                    >  2:1021115/1‑101 (MQ=255)
                  tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                                    <  1:3006872/101‑1 (MQ=255)
                  tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGACGGTCCGGGCTTCGTTTCATC.TTGCCCCa                                                                                    >  2:3248387/1‑101 (MQ=18)
                   gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                                                   >  1:161997/1‑101 (MQ=255)
                    aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTTGCCCCaa                                                                                   >  2:3207103/1‑101 (MQ=2)
                    aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                                  <  2:1736919/101‑1 (MQ=255)
                     ggCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                                 <  2:2621547/101‑1 (MQ=255)
                      gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                                <  1:4201899/101‑1 (MQ=255)
                      gCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                                >  2:616582/1‑101 (MQ=255)
                        cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATc                                                                              >  2:2813133/1‑101 (MQ=255)
                         gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                             >  1:3722448/1‑101 (MQ=255)
                         gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                             <  2:4264487/101‑1 (MQ=255)
                         gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                             <  1:2185617/101‑1 (MQ=255)
                         gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                                                             >  1:4312483/1‑101 (MQ=255)
                          ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATccc                                                                            <  2:351802/101‑1 (MQ=255)
                           gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                                >  1:3063551/4‑100 (MQ=2)
                           gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttg                                                                <  1:4617392/98‑2 (MQ=2)
                           gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCg                                                                           <  1:19822/101‑1 (MQ=255)
                            acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttgg                                                               >  1:2229861/3‑99 (MQ=2)
                             cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttggc                                                              >  2:4559270/2‑98 (MQ=2)
                             aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAATCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                                                                         >  2:4638799/1‑101 (MQ=255)
                              ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCgggg                                                                        >  1:4644551/1‑101 (MQ=255)
                                 gggCGCTGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                                     <  1:503068/101‑1 (MQ=255)
                                  ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGa                                                                    <  2:757808/101‑1 (MQ=25)
                                    ggcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                                                                  <  1:2929229/100‑1 (MQ=255)
                                     gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                                                                 <  2:2827217/101‑1 (MQ=255)
                                     gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAtt                                                                 >  1:750196/1‑101 (MQ=255)
                                      cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAttt                                                                >  2:1537481/1‑101 (MQ=255)
                                        gCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTg                                                              >  1:4387358/1‑101 (MQ=255)
                                          cccccGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGgc                                                            >  2:2009999/1‑101 (MQ=255)
                                              cgtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                                                        >  1:157086/5‑99 (MQ=255)
                                               gtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                       <  1:4540420/98‑1 (MQ=255)
                                                 ccTGCGGACTCCCCCCGGGAT.ATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGGATTTTGGCGCAGCCa                                                     >  2:1576406/2‑101 (MQ=255)
                                                 ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                     <  2:4066967/100‑1 (MQ=255)
                                                 ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                     <  1:4351667/100‑1 (MQ=255)
                                                 ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                     <  1:3360966/100‑1 (MQ=255)
                                                  cTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                    >  2:2972446/1‑101 (MQ=255)
                                                   tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                   <  2:1628959/101‑1 (MQ=255)
                                                      ggACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                                >  1:2011061/1‑101 (MQ=255)
                                                       gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                               <  1:2740740/101‑1 (MQ=255)
                                                       gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                               >  1:2293202/1‑101 (MQ=255)
                                                          tCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                            <  1:2418742/101‑1 (MQ=255)
                                                           ccccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                           >  2:1787468/1‑101 (MQ=255)
                                                             ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                         >  2:3830828/1‑101 (MQ=255)
                                                             ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                         <  2:846274/101‑1 (MQ=255)
                                                              cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                        >  2:783530/1‑101 (MQ=255)
                                                               ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                       <  1:899342/101‑1 (MQ=255)
                                                                cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                      >  2:2521322/1‑101 (MQ=255)
                                                                 gggAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                     >  2:2174067/1‑101 (MQ=255)
                                                                   gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                   >  2:442442/1‑101 (MQ=255)
                                                                    at.atTGGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                  <  2:273260/101‑1 (MQ=255)
                                                                       atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                <  2:419522/101‑1 (MQ=255)
                                                                       atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                >  1:3816961/1‑101 (MQ=255)
                                                                       atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                <  1:2813349/101‑1 (MQ=255)
                                                                       atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                <  1:1769233/101‑1 (MQ=255)
                                                                        tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                               >  1:1184163/1‑101 (MQ=255)
                                                                        tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                               >  1:1121381/1‑101 (MQ=255)
                                                                         ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                              >  2:168727/1‑101 (MQ=255)
                                                                          tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                             >  2:1285456/1‑101 (MQ=255)
                                                                            aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                           >  2:3631547/1‑101 (MQ=255)
                                                                             ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                          <  2:332713/101‑1 (MQ=255)
                                                                              gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                         <  2:4167242/101‑1 (MQ=255)
                                                                                aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                       >  1:1025979/1‑101 (MQ=255)
                                                                                      aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                 >  2:2052682/1‑101 (MQ=255)
                                                                                      aaaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg                 >  2:631590/1‑101 (MQ=255)
                                                                                       aaGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                >  1:1878708/1‑101 (MQ=255)
                                                                                        aGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg               <  2:3251377/101‑1 (MQ=255)
                                                                                        aGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg               >  2:1497541/1‑101 (MQ=255)
                                                                                         gCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAACATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcgg              <  1:3973305/101‑1 (MQ=255)
                                                                                             aTCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTgcggcgcggcggcgcg          >  1:1548959/1‑101 (MQ=255)
                                                                                               ccGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc        <  1:4064135/101‑1 (MQ=255)
                                                                                                cGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCACGCGccgcggcggcgcggcg       >  2:1398324/1‑101 (MQ=255)
                                                                                                 gggCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc      >  2:916799/1‑101 (MQ=255)
                                                                                                 gggCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc      >  1:3261309/1‑101 (MQ=255)
                                                                                                 gggCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc      >  2:375567/1‑101 (MQ=255)
                                                                                                   gCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg    <  1:2584067/101‑1 (MQ=255)
                                                                                                   gCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg    >  2:3436688/1‑101 (MQ=255)
                                                                                                    cTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGc   >  2:3890804/1‑101 (MQ=255)
                                                                                                     ttCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc  >  1:4479304/1‑101 (MQ=255)
                                                                                                     ttCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc  <  2:1863537/101‑1 (MQ=255)
                                                                                                     |                                                                                                     
GCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCC  >  NC_014034/432377‑432576

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG