Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,493 C→G 3.7% intergenic (+96/+52) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4930C→G 3.7% 7.7 ‑1.4 ‑0.0188intergenic (+96/+52)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (103/78):  total (109/79)
Fisher's exact test strand distribution p-value = 2.42e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.42e-01

Only 100 of 200 total aligned reads displayed.

TGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGC  >  NC_014034/432395‑432593
                                                                                                      |                                                                                                    
tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCa                                                                                                     <  1:3006872/101‑1 (MQ=255)
tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCa                                                                                                     >  2:1021115/1‑101 (MQ=255)
tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGACGGTCCGGGCTTCGTTTCATC.TT.GCCCCa                                                                                                     >  2:3248387/1‑101 (MQ=18)
 gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaa                                                                                                    >  1:161997/1‑101 (MQ=255)
  aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGGTAATTTAAGGAAAAATAAAACCGGACCGGGGCTTCGTTT.ATCTTT.GCCCCaa                                                                                                    >  2:3207103/1‑101 (MQ=2)
  aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaaa                                                                                                   <  2:1736919/101‑1 (MQ=255)
  aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCaaa                                                                                                   >  1:4534122/1‑101 (MQ=255)
      cGGGCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATc                                                                                               >  1:2735875/1‑101 (MQ=255)
       gggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAAATGAAAGCAGATCCGGGCTTCGTTTCATC.TT.TCCCCAAatatac                                                                                              >  1:1176229/1‑99 (MQ=18)
        ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAtt                                                                                  >  1:1996360/5‑101 (MQ=2)
        ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAtt                                                                                  >  1:4436668/5‑101 (MQ=2)
        ggCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATccc                                                                                             <  2:351802/101‑1 (MQ=255)
         gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttg                                                                                 >  1:3063551/4‑100 (MQ=2)
         gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGAT.ATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TT.GCCCGAAATATCCCAGGGGTGAAttg                                                                                 >  1:2388947/4‑100 (MQ=2)
          cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCgg                                                                                           >  2:4588357/1‑101 (MQ=255)
           aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCggg                                                                                          >  1:486387/1‑101 (MQ=255)
           aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCGTCGTTTCATC.TT.GCCCCAAATATCCCggg                                                                                          >  2:2142120/1‑101 (MQ=255)
            ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCgggg                                                                                         >  1:4644551/1‑101 (MQ=255)
                ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGa                                                                                     <  2:1673987/101‑1 (MQ=255)
                ggCGCCGCCCCCCGTCCTGCGGCCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGa                                                                                     <  2:757808/101‑1 (MQ=25)
                  ggcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAt                                                                                   <  1:2929229/100‑1 (MQ=255)
                  cgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAt                                                                                   <  2:2824239/101‑1 (MQ=255)
                   ccTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAtt                                                                                  <  2:2867068/100‑1 (MQ=255)
                    cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAttt                                                                                 <  2:2282586/101‑1 (MQ=255)
                    cTGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGGGAAttt                                                                                 >  2:1537481/1‑101 (MQ=255)
                     tGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAtttt                                                                                >  2:4164595/1‑101 (MQ=255)
                     tGCCCCCCGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAAtttt                                                                                >  1:2219773/1‑101 (MQ=255)
                       ccccccGTCCTGCGGACCCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTgg                                                                              <  2:4189086/101‑1 (MQ=255)
                        cccccGTCCTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGgc                                                                             >  2:2009999/1‑101 (MQ=255)
                         ccccGTCCTGCGGACTCCCCCCGGGAT.ATTTTAGGGAAGAAGAAAACCGATCCCGGGCTCGTTTCATC.TTGGCCCAAAAAATCCCGGGGGGGAGTTTTgcg                                                                             >  2:3973020/1‑99 (MQ=12)
                            cgtccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                                                                         >  1:157086/5‑99 (MQ=255)
                              tccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                                       <  2:161983/99‑1 (MQ=255)
                               ccTGCGGACTCCCCCCGGGAT.ATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGGGGATTTTGGCGCAGCCa                                                                      >  2:1576406/2‑101 (MQ=255)
                               ccTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                      <  1:3360966/100‑1 (MQ=255)
                                cTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                                     >  2:2972446/1‑101 (MQ=255)
                                 tGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                                    <  2:2013252/101‑1 (MQ=255)
                                     gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                >  2:4551371/1‑101 (MQ=255)
                                     gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                <  1:833995/101‑1 (MQ=255)
                                     gACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                <  2:3018378/101‑1 (MQ=255)
                                      aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                               <  2:389526/101‑1 (MQ=255)
                                      aCTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                               >  1:1345510/1‑101 (MQ=255)
                                        tCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                                             <  2:4289789/101‑1 (MQ=255)
                                         ccccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggggg                                                            >  2:1787468/1‑101 (MQ=255)
                                          cccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                                           <  2:2743097/101‑1 (MQ=255)
                                           ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                                          <  2:846274/101‑1 (MQ=255)
                                           ccccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                                          >  2:3830828/1‑101 (MQ=255)
                                            cccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAg                                                         >  2:783530/1‑101 (MQ=255)
                                             ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                        <  1:899342/101‑1 (MQ=255)
                                             ccGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                        <  1:197290/101‑1 (MQ=255)
                                              cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                       >  2:2521322/1‑101 (MQ=255)
                                              cGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                       >  1:2282133/1‑101 (MQ=255)
                                                ggAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgcg                                                     <  1:4588656/101‑2 (MQ=255)
                                                 gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                    <  1:4382486/101‑1 (MQ=255)
                                                 gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                    >  1:4047097/1‑101 (MQ=255)
                                                 gAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                    >  2:442442/1‑101 (MQ=255)
                                                  at.atTTGAGGCAAGATGAAAACCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                                   >  2:595535/1‑101 (MQ=255)
                                                     atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                 <  1:1769233/101‑1 (MQ=255)
                                                     atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                 >  1:3816961/1‑101 (MQ=255)
                                                         gAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcc                                             >  1:208632/1‑101 (MQ=255)
                                                          aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                                            >  2:3631547/1‑101 (MQ=255)
                                                          aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc                                            >  1:2836223/1‑101 (MQ=255)
                                                           ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                           <  2:332713/101‑1 (MQ=255)
                                                            gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                          >  2:2731098/1‑101 (MQ=255)
                                                            gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                          <  1:4324902/101‑1 (MQ=255)
                                                            gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                          <  2:943700/101‑1 (MQ=255)
                                                              aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                                        >  1:1025979/1‑101 (MQ=255)
                                                                    aaaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                  >  1:4188969/1‑101 (MQ=255)
                                                                    aaaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg                                  >  2:631590/1‑101 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                 >  2:1058500/1‑101 (MQ=255)
                                                                     aaGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                 >  1:1878708/1‑101 (MQ=255)
                                                                      aGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                >  2:1497541/1‑101 (MQ=255)
                                                                      aGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                >  2:4251532/1‑101 (MQ=255)
                                                                      aGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                <  2:3251377/101‑1 (MQ=255)
                                                                           aTCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTgcggcgcggcggcgcg                           >  1:1548959/1‑101 (MQ=255)
                                                                           aTCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCACCccgacgcggcggagcg                           >  1:3715506/1‑101 (MQ=21)
                                                                             ccGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc                         <  1:4064135/101‑1 (MQ=255)
                                                                              cGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCACGCGccgcggcggcgcggcg                        >  2:1398324/1‑101 (MQ=255)
                                                                               gggCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc                       >  1:3261309/1‑101 (MQ=255)
                                                                               gggCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc                       >  2:375567/1‑101 (MQ=255)
                                                                                 gCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                     >  2:3436688/1‑101 (MQ=255)
                                                                                 gCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                     <  1:2584067/101‑1 (MQ=255)
                                                                                 gCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                     <  2:2599241/101‑1 (MQ=255)
                                                                                   ttCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGcc                   <  2:1863537/101‑1 (MQ=255)
                                                                                       tttCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc               >  1:2486426/1‑101 (MQ=255)
                                                                                       tttCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc               >  2:2398769/1‑101 (MQ=255)
                                                                                       tttCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc               >  1:1777229/1‑101 (MQ=255)
                                                                                       tttCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc               >  2:1328443/1‑101 (MQ=255)
                                                                                         tCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGt             >  2:2105265/1‑101 (MQ=255)
                                                                                          cATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt            >  1:3980481/1‑101 (MQ=255)
                                                                                          cATC.TT.CCCCCAAATACCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt            <  1:3187962/101‑1 (MQ=255)
                                                                                           aTC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTg           >  1:4295704/1‑101 (MQ=255)
                                                                                           aTC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTg           >  2:1276534/1‑101 (MQ=255)
                                                                                            tC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGa          >  1:4634875/1‑101 (MQ=255)
                                                                                             c.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGAt         >  1:2809562/1‑101 (MQ=255)
                                                                                               tt.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATc        >  1:4290072/1‑101 (MQ=255)
                                                                                               tt.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATc        >  1:1896121/1‑101 (MQ=255)
                                                                                                  gCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgc      <  1:660803/101‑1 (MQ=255)
                                                                                                   ccccAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcg     >  1:2325809/1‑101 (MQ=255)
                                                                                                     ccAAATATCCCGGGGGCGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgcg   >  1:3698447/1‑101 (MQ=255)
                                                                                                      cAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCTCGGCGGCGCGGCGCAGCCGGTCGTTGATCgcgagc  >  1:3875628/1‑101 (MQ=255)
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TGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGAT.ATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TT.GCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGC  >  NC_014034/432395‑432593

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG