Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,503 G→A 3.7% intergenic (+106/+42) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,5030G→A 3.7% 9.6 ‑1.4 ‑0.0186intergenic (+106/+42)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/1):  ref base (107/72):  total (113/73)
Fisher's exact test strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.85e-01

Only 100 of 196 total aligned reads displayed.

GGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCG  >  NC_014034/432403‑432602
                                                                                                     |                                                                                                   
ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                                           >  1:4436668/5‑101 (MQ=2)
 gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttg                                                                                          >  1:3063551/4‑100 (MQ=2)
 gacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttg                                                                                          <  1:4617392/98‑2 (MQ=2)
 gCAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCg                                                                                                     <  1:19822/101‑1 (MQ=255)
  acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                                                         >  1:2229861/3‑99 (MQ=2)
   cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                                        >  2:4559270/2‑98 (MQ=2)
   aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAATCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                                                   >  2:4638799/1‑101 (MQ=255)
   aGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCggg                                                                                                   >  1:486387/1‑101 (MQ=255)
    ggggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCgggg                                                                                                  >  1:4644551/1‑101 (MQ=255)
      ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGt                                                                                                <  2:179948/101‑1 (MQ=255)
        ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                              >  1:1890163/1‑101 (MQ=255)
           gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                                           <  2:2827217/101‑1 (MQ=255)
           gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAAtt                                                                                           >  1:750196/1‑101 (MQ=255)
           ccTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                                           <  2:2867068/100‑1 (MQ=255)
            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                                          >  2:2511118/1‑101 (MQ=255)
            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAttt                                                                                          <  1:3269642/101‑1 (MQ=255)
            cTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGAAttt                                                                                          >  2:1537481/1‑101 (MQ=255)
             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                         >  2:4164595/1‑101 (MQ=255)
               ccccccGTCCTGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTgg                                                                                       <  2:4189086/101‑1 (MQ=255)
                    cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTTgcgccg                                                                                  >  1:157086/5‑99 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                 <  1:4540420/98‑1 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                 <  2:1144797/98‑1 (MQ=255)
                       ccTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGGGGATTTTGGCGCAGCCa                                                                               >  2:1576406/2‑101 (MQ=255)
                       ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                               >  1:1863663/2‑101 (MQ=255)
                         tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                                             <  2:1628959/101‑1 (MQ=255)
                         tGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaa                                                                             >  2:758115/1‑101 (MQ=255)
                          gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                                            <  1:4290940/101‑1 (MQ=255)
                            ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                                                          >  1:2011061/1‑101 (MQ=255)
                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                         <  1:2740740/101‑1 (MQ=255)
                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                         <  2:3018378/101‑1 (MQ=255)
                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                         >  1:2293202/1‑101 (MQ=255)
                              aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                                        >  2:3787760/1‑101 (MQ=255)
                              aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                                        >  1:1345510/1‑101 (MQ=255)
                                tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                                                      <  1:2418742/101‑1 (MQ=255)
                                  cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                                                    <  2:2743097/101‑1 (MQ=255)
                                     ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                                 <  1:899342/101‑1 (MQ=255)
                                      cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTGCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                                >  2:2377296/1‑101 (MQ=255)
                                       gggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcgc                                                               >  2:2174067/1‑101 (MQ=255)
                                         gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                             >  1:4047097/1‑101 (MQ=255)
                                         gATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccc                                                             <  1:4382486/101‑1 (MQ=255)
                                           tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                                                           >  1:2873087/1‑101 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                          >  1:3816961/1‑101 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                          <  1:2813349/101‑1 (MQ=255)
                                             tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                                         >  1:1121381/1‑101 (MQ=255)
                                              ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                                        >  2:168727/1‑101 (MQ=255)
                                              ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                                        >  2:4479727/1‑101 (MQ=255)
                                               tGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                                       >  2:1285456/1‑101 (MQ=255)
                                                  ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                                    <  2:332713/101‑1 (MQ=255)
                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                                   <  1:4324902/101‑1 (MQ=255)
                                                   gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                                   >  2:2731098/1‑101 (MQ=255)
                                                     aaGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg                                                 >  1:1025979/1‑101 (MQ=255)
                                                      aGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                                                >  1:855383/1‑101 (MQ=255)
                                                       gaggAAAGCCGATCCGGGCTTCGTTTCATCTTTCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                                               <  2:4400977/98‑1 (MQ=255)
                                                        aTGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg                                              >  1:1769544/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                            >  1:1659956/1‑101 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                           >  2:255172/1‑101 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                           >  2:2052682/1‑101 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                           <  2:1039238/101‑1 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                           >  1:4188969/1‑101 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggagcgg                                           >  2:631590/1‑101 (MQ=255)
                                                             aGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                         >  2:1497541/1‑101 (MQ=255)
                                                             aGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                         >  2:4251532/1‑101 (MQ=255)
                                                             aGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcg                                         <  2:3251377/101‑1 (MQ=255)
                                                               ccGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                                       >  2:3907041/1‑101 (MQ=255)
                                                                 gATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcgc                                     <  1:250960/101‑1 (MQ=255)
                                                                  aTCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCTgcggcgcggcggcgcg                                    >  1:1548959/1‑101 (MQ=255)
                                                                    ccGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc                                  <  1:4064135/101‑1 (MQ=255)
                                                                    ccGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc                                  <  2:801635/101‑1 (MQ=255)
                                                                     cGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg                                 <  2:1890037/101‑1 (MQ=255)
                                                                     cGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGgcgcggcggcgcggcg                                 >  1:2341555/1‑101 (MQ=255)
                                                                     cGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCACGCGccgcggcggcgcggcg                                 >  2:1398324/1‑101 (MQ=255)
                                                                      gggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCggcgcggcgc                                >  2:916799/1‑101 (MQ=255)
                                                                       ggCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCa                               <  2:3154129/101‑1 (MQ=255)
                                                                        gCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                              <  1:2584067/101‑1 (MQ=255)
                                                                        gCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                              >  2:3436688/1‑101 (MQ=255)
                                                                         cTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGc                             >  2:3606671/1‑101 (MQ=255)
                                                                         cTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGc                             >  2:3890804/1‑101 (MQ=255)
                                                                               ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg                       <  2:2010921/101‑1 (MQ=255)
                                                                                tCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGt                      >  1:3429736/1‑101 (MQ=255)
                                                                                tCATCTTGCCCCAAATATCCCGGGGGCGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCAGCGCGGCGGCGCGGCGCAGCCGGTCGt                      >  1:197052/1‑101 (MQ=255)
                                                                                 cATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt                     >  1:3980481/1‑101 (MQ=255)
                                                                                 cATCTTCCCCCAAATACCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt                     <  1:3187962/101‑1 (MQ=255)
                                                                                   tCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGa                   >  1:4634875/1‑101 (MQ=255)
                                                                                    cTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGAt                  <  1:3248632/101‑1 (MQ=255)
                                                                                    cTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGAt                  >  2:3666330/1‑101 (MQ=255)
                                                                                       gCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgc               <  1:660803/101‑1 (MQ=255)
                                                                                        ccccAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgac              >  2:2110855/1‑99 (MQ=255)
                                                                                          ccAAATATCCCGGGGGCGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcgcg            >  1:3698447/1‑101 (MQ=255)
                                                                                           cAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCTCGGCGGCGCGGCGCAGCCGGTCGTTGATCgcgagc           >  1:3875628/1‑101 (MQ=255)
                                                                                            aaaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGcc          >  2:1752271/1‑101 (MQ=255)
                                                                                            aaaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCCcc          >  1:3150999/1‑101 (MQ=255)
                                                                                            aaaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGGCGTTGATCGCGCGcc          >  1:4498815/1‑101 (MQ=255)
                                                                                             aaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGccc         >  2:2722613/1‑101 (MQ=255)
                                                                                              atatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCa        <  1:3716407/101‑1 (MQ=255)
                                                                                              atatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCa        <  1:2972675/101‑1 (MQ=255)
                                                                                              atatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCa        >  2:1338364/1‑101 (MQ=255)
                                                                                               tatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgcggcgcatc       >  2:2987215/1‑94 (MQ=255)
                                                                                               tatCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCaa       <  1:2142270/101‑1 (MQ=255)
                                                                                                  cccGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGcc    >  2:4044334/1‑101 (MQ=255)
                                                                                                   ccGGTGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGGGCGGCGCAGCCGGTCGCTGATCGCGCGCCCAAGccc   >  1:2085058/1‑101 (MQ=255)
                                                                                                     |                                                                                                   
GGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCG  >  NC_014034/432403‑432602

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG