Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 530,947 T→C 3.2% intergenic (+25/+116) valS → / ← RCAP_rcc00497 valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034530,9470G→C 3.2% 2.3 ‑0.9 ‑0.1133intergenic (+25/+116)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (3/2):  ref base (50/75):  total (53/80)
Fisher's exact test strand distribution p-value = 3.97e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.71e-01

Only 100 of 139 total aligned reads displayed.

GAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..C.CGCGCTTGTC.GGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530869‑531022
                                                                                 |                                                                            
gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATAtt                                                        <  2:4538467/101‑1 (MQ=25)
 aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATAttt                                                       >  1:3068607/1‑101 (MQ=25)
 aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATAttt                                                       >  1:1177493/1‑101 (MQ=25)
       cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:2387482/101‑1 (MQ=25)
       cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:436719/101‑1 (MQ=25)
        ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCaa                                                >  1:2894617/1‑101 (MQ=25)
        ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGCATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCaa                                                <  2:4624778/101‑1 (MQ=21)
         gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGGTC..T.GCCCCCCGCCTCGCTCCCCCCCGGGTATTTTCGGCCAg                                               >  2:2982352/1‑101 (MQ=255)
         gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAg                                               <  1:881726/101‑1 (MQ=25)
          cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  2:4085854/101‑1 (MQ=21)
          cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGa                                              >  2:3694727/1‑101 (MQ=21)
           gAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CCCTCCCCCCGTGATATTTTCGGCAAGAt                                             >  2:1229801/1‑101 (MQ=21)
            aaGATGGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  2:1727916/101‑1 (MQ=21)
            aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  1:4401618/1‑101 (MQ=21)
            aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  1:4083430/1‑101 (MQ=21)
            aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  1:3253972/1‑101 (MQ=21)
             aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           <  2:219589/101‑1 (MQ=21)
              gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  2:3627311/101‑1 (MQ=21)
              gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  2:1273167/101‑1 (MQ=21)
               aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGTATATTTTCGGCAAGATAAAg                                         <  2:309875/101‑1 (MQ=21)
               aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGCGACATTTTTGGCAAAAGAAAg                                         >  1:1653537/1‑101 (MQ=16)
                  ggcgcggcgcTGAAGCGTCTGGCCGACCTCGGATAAAGGAAGAGGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  1:1569852/101‑1 (MQ=21)
                  ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGGGATTTTCTGCCAGATAAAgggc                                      >  2:2658836/1‑99 (MQ=16)
                  ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  2:1107127/101‑1 (MQ=21)
                  ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      <  1:2641917/101‑1 (MQ=21)
                   gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  1:1158858/101‑1 (MQ=21)
                   gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  2:1884102/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:1975556/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:2543673/101‑1 (MQ=21)
                     gcggcgcTGACGCGTCTGGCCGAGCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  2:2256939/101‑1 (MQ=18)
                     gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  1:2714054/101‑1 (MQ=21)
                     gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   >  2:2789860/1‑101 (MQ=21)
                     gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  2:1317845/101‑1 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGGTCT.G.CCCCCCCCTT.CGCCCCCCCCCGGAAATTTTTGGCAAGATAAAAGCCCggg                                  >  1:604588/1‑100 (MQ=255)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  >  2:3779141/1‑101 (MQ=21)
                       ggctcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.C.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:1115098/101‑1 (MQ=25)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 >  1:3934545/1‑101 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 >  1:2412500/1‑101 (MQ=21)
                        gcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCGCT.G.CCCCCCGCTT.CGCTCCCCCCGGGAGATTTTCGGCAAGATAAAGGCCCGCAAg                                <  2:1197661/101‑1 (MQ=19)
                          gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:301012/1‑101 (MQ=21)
                          gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              <  1:65458/101‑1 (MQ=21)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             <  2:742322/101‑1 (MQ=21)
                            tGAAGGGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGCCTCT.C.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:1577216/101‑1 (MQ=21)
                            tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            >  2:2117503/1‑101 (MQ=21)
                            tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:2497324/101‑1 (MQ=21)
                            tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:2982581/101‑1 (MQ=21)
                            tGAAGCATCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            <  1:755867/101‑1 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAGGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  1:3217885/101‑1 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  2:1455197/1‑101 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  1:3417262/101‑1 (MQ=21)
                              aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:1642297/1‑101 (MQ=21)
                              aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:4214030/1‑101 (MQ=21)
                                 cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  2:1015609/101‑1 (MQ=21)
                                 cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  2:3001441/101‑1 (MQ=21)
                                  gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      >  2:2181526/1‑101 (MQ=21)
                                  gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      <  2:604527/101‑1 (MQ=21)
                                   tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     <  1:4508350/101‑1 (MQ=21)
                                   tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     >  1:3759843/1‑101 (MQ=21)
                                    cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    >  1:3676508/1‑101 (MQ=21)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  2:837614/101‑1 (MQ=21)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  1:4155317/101‑1 (MQ=21)
                                      ggCCGAACTCGGAAAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCtt                  <  1:2408635/101‑1 (MQ=21)
                                       gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCTCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  1:3847062/101‑1 (MQ=25)
                                       gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 >  2:2735595/1‑101 (MQ=21)
                                       gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  2:2531622/101‑1 (MQ=21)
                                       gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  1:4367396/101‑1 (MQ=21)
                                       gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  1:4486101/101‑1 (MQ=21)
                                       gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 <  2:666417/101‑1 (MQ=21)
                                        ccGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGc                >  2:2678673/1‑101 (MQ=21)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCACGATAAAGGCCCGGAANACTCCCTTTCTCttttc               >  2:1366197/1‑98 (MQ=16)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               <  1:614986/101‑1 (MQ=21)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               >  2:2297344/1‑101 (MQ=21)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTC..CGCCCCCCGCTT.CGCTCCCCCCGGGACATTTTCGGCAAGATAAAAGGCCGGAAGCCTCCCTTTATCTTgtc               >  2:2076000/1‑99 (MQ=19)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  1:4377828/1‑101 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  1:582375/1‑101 (MQ=21)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCTCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             <  1:239817/101‑1 (MQ=25)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  1:2743501/1‑101 (MQ=21)
                                            aCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            >  2:2421662/1‑101 (MQ=21)
                                            aCTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            <  1:2619214/101‑1 (MQ=21)
                                             cTCGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaa           >  1:1643138/1‑101 (MQ=21)
                                               gggATAAAGGAAGACGGGGGGCTCTGC.CCCCCGTTTC..GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  1:4027584/100‑1 (MQ=25)
                                               cGGATAAAGGAAGAGGGGGGGCTCT.G.CCCCCCCCTT.CGCT.CCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:4236658/101‑1 (MQ=19)
                                               cGGATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  1:180299/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.G.CTCCCCGCTT.CGCTCCCCCCTGGATATTTTCGGCAAGATAAAGGCCCGGAACCCTCCCTTTATCTTGCCGGAAata        <  1:3921073/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        >  1:3464907/1‑101 (MQ=21)
                                                 gATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:1747275/101‑1 (MQ=21)
                                                 gATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       >  2:1558132/1‑101 (MQ=21)
                                                 gATAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  2:3336751/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATTTc      <  1:2659021/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      >  2:4079216/1‑101 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:2289833/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      >  2:1571108/1‑101 (MQ=21)
                                                   tAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  2:2773846/101‑1 (MQ=21)
                                                   tAAAGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  1:1118151/101‑1 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  2:1500126/1‑101 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    <  1:1103256/101‑1 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:494592/101‑1 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   >  1:1129140/1‑101 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.G.CCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGCCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   >  1:3248470/1‑101 (MQ=21)
                                                      aGGAAGAAGGGGGGCTCTGC.CCCCCGCTTC..GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCaa  >  2:184643/1‑101 (MQ=21)
                                                                                 |                                                                            
GAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..C.CGCGCTTGTC.GGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530869‑531022

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG