Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 580,957 C→A 2.1% intergenic (+78/‑293) RCAP_rcc00541 → / → RCAP_rcc00542 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034580,9570C→A 2.1% 2.6 0.0 0.0203intergenic (+78/‑293)RCAP_rcc00541/RCAP_rcc00542hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (86/112):  total (88/115)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 214 total aligned reads displayed.

GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGC  >  NC_014034/580862‑581057
                                                                                                |                                                                                                    
  gtTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGgcgc                                                                                                <  1:3203183/101‑1 (MQ=255)
    tCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCt                                                                                              <  1:2444955/101‑1 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                             <  1:2817895/101‑1 (MQ=255)
     ccGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTa                                                                                             <  2:4382497/101‑1 (MQ=255)
      cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                            <  1:4304149/101‑1 (MQ=255)
      cGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTAt                                                                                            <  1:2372471/101‑1 (MQ=255)
       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGGAATTCACCCCCGGGGTATTTGAGGCAAGATAAACGGGCGGCTATc                                                                                           >  2:1347439/1‑101 (MQ=25)
       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGGTATTTTAGGCAAGATAAACGGGCGCCCATc                                                                                           >  1:4197468/1‑101 (MQ=25)
       gCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATc                                                                                           >  2:585349/1‑101 (MQ=255)
         cTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCAc                                                                                         <  1:691563/101‑1 (MQ=255)
          tCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCCAAATTCACCCCCCGGGTATTTTAGGCAAGATAAACGGGCGCCTATCAcc                                                                                        >  2:4223877/1‑101 (MQ=25)
            gATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCtt                                                                                      >  2:3463688/1‑101 (MQ=255)
             aTTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTg                                                                                     <  2:906563/101‑1 (MQ=255)
               tGAGGCGGCCCGGGCAGGGGGCGCTTCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAAAGGGCGCCTATCACCTTGGt                                                                                   >  1:3075514/1‑101 (MQ=21)
               tGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTggg                                                                                   >  2:4540024/1‑100 (MQ=255)
                   gcggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaa                                                                               <  2:145985/101‑1 (MQ=255)
                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCaaaa                                                                             >  2:3559852/1‑100 (MQ=255)
                     ggcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAat                                                                             <  2:1224572/101‑1 (MQ=255)
                      gcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCTTATCACCTTGGTGCAAata                                                                            <  1:3510833/101‑1 (MQ=255)
                      gcCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAata                                                                            <  1:344892/101‑1 (MQ=255)
                       cccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAatat                                                                           <  1:102150/101‑1 (MQ=255)
                        ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAGATTCACCCCCGGGATATTTGAGGCAAGATAAAAGGGAGCCTATCACCTTGGTGCAAATATc                                                                          >  1:1769656/1‑101 (MQ=21)
                        ccGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATc                                                                          <  1:922778/101‑1 (MQ=255)
                         cGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATcc                                                                         >  2:750181/1‑101 (MQ=255)
                         cGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATcc                                                                         <  2:3615290/101‑1 (MQ=255)
                          gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                        >  1:3348229/1‑101 (MQ=255)
                          gggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCg                                                                        >  2:3887777/1‑101 (MQ=255)
                           ggCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgg                                                                       <  2:3269465/101‑1 (MQ=255)
                            gCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggg                                                                      <  2:2252775/101‑1 (MQ=255)
                              aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                                    >  2:4496845/1‑101 (MQ=255)
                              aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCggggg                                                                    >  2:3731619/1‑101 (MQ=255)
                              aGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCAACCCCCGGATATTTGAGGCAAGATAAACGGGCGCCTATTACCTTGGGGCAAATATCCggggg                                                                    >  2:2024094/1‑101 (MQ=21)
                               gggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCgggggg                                                                   >  2:1158691/1‑101 (MQ=255)
                                ggggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGt                                                                  <  1:1083698/101‑1 (MQ=255)
                                  ggCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGa                                                                <  1:2604728/101‑1 (MQ=255)
                                   gcgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                               <  1:4574726/101‑1 (MQ=255)
                                   gcgcTGCCCCCTCTTTCGGCTGCGCCGAAAGTCTCCCCCGTGTTATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAg                                                               <  2:1836180/101‑1 (MQ=255)
                                    cgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAgg                                                              <  2:2891156/101‑1 (MQ=255)
                                    cgcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAgg                                                              >  1:3509792/1‑101 (MQ=255)
                                     gcTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGc                                                             <  1:4135966/101‑1 (MQ=255)
                                        gCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                          <  2:4093598/101‑1 (MQ=255)
                                        gCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGa                                                          <  1:2089028/101‑1 (MQ=255)
                                        gCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGa                                                          >  2:2703886/1‑101 (MQ=255)
                                          ccccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGaaa                                                        >  2:3037926/1‑101 (MQ=255)
                                            ccTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGc                                                      >  2:3515928/1‑101 (MQ=255)
                                             ctctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGcc                                                     <  2:308910/101‑1 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                    >  1:4649040/1‑101 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                    <  1:4500875/101‑1 (MQ=255)
                                              tctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCg                                                    >  2:1078434/1‑101 (MQ=255)
                                               ctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                   <  1:2673642/101‑1 (MQ=255)
                                               ctTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGa                                                   >  2:3421939/1‑101 (MQ=255)
                                                  tCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggg                                                >  2:2324136/1‑101 (MQ=255)
                                                  tCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATACGGGGGGTGAGGCCGAAAGCCGAggg                                                >  1:1184544/1‑101 (MQ=255)
                                                   cGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                               >  1:579750/1‑101 (MQ=255)
                                                   cGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                               <  1:1680534/101‑1 (MQ=255)
                                                   cGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAgggg                                               >  1:2925820/1‑101 (MQ=255)
                                                    ggCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAggggg                                              <  1:3314701/101‑1 (MQ=255)
                                                     gCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                             <  2:579689/101‑1 (MQ=255)
                                                     gCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGc                                             >  1:301584/1‑101 (MQ=255)
                                                      cTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                            <  2:3404567/101‑1 (MQ=255)
                                                      cTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCa                                            >  2:4471389/1‑101 (MQ=255)
                                                       tGCGCCGAAATTCCCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                           <  1:1069360/101‑1 (MQ=255)
                                                       tGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAg                                           >  2:4001377/1‑101 (MQ=255)
                                                         cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                         <  1:4335661/101‑1 (MQ=255)
                                                         cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                         <  1:2132291/101‑1 (MQ=255)
                                                         cgcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcg                                         <  2:2463333/101‑1 (MQ=255)
                                                          gcCGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAgcgc                                        >  2:2887734/1‑101 (MQ=255)
                                                            cGAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccc                                      >  1:4176173/1‑101 (MQ=255)
                                                             gAAATTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGcccc                                     >  2:138664/1‑101 (MQ=255)
                                                              aaaTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGccccc                                    <  2:3768287/101‑1 (MQ=255)
                                                                aTTCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtt                                  <  2:2767391/101‑1 (MQ=255)
                                                                 tgccggctcGGGTTATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAATCCGAGGGGGGAGTGCCCCCttt                                 <  2:2187836/93‑1 (MQ=37)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                >  2:2957743/1‑101 (MQ=255)
                                                                  tCACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCtttt                                <  2:2397483/101‑1 (MQ=255)
                                                                   cACCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTc                               >  1:2515998/1‑101 (MQ=255)
                                                                    aCCCCCGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTcc                              >  2:1272971/1‑101 (MQ=255)
                                                                         cGGGATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTTGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcg                         >  1:1517456/1‑101 (MQ=255)
                                                                          gggATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCcgtcgt                        >  1:6042/1‑101 (MQ=255)
                                                                            gATATTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCgtgt                      <  2:1769537/101‑1 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGGGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                    >  2:2985393/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                    >  1:3788591/1‑101 (MQ=255)
                                                                              tatTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTgg                    >  1:2167611/1‑101 (MQ=255)
                                                                               atTTGAGGCAAGATAAACGGGCGCCTATCCCCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                   <  2:1254820/101‑1 (MQ=255)
                                                                               atTTGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGa                   <  1:4490777/101‑1 (MQ=255)
                                                                                 ttGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCTGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                 >  2:874673/1‑101 (MQ=255)
                                                                                 ttGAGGCAAGATAAACGGGCGCCTATCACCGTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                 <  1:3847281/101‑1 (MQ=255)
                                                                                 ttGAGGCAAGATAAACGCGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATc                 <  2:4194542/101‑1 (MQ=255)
                                                                                  tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                <  2:945202/101‑1 (MQ=255)
                                                                                  tGAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcg                <  1:3649817/101‑1 (MQ=255)
                                                                                   gAGGCAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATcgc               <  1:96422/101‑1 (MQ=255)
                                                                                       cAAGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCaa           <  1:1602360/101‑1 (MQ=255)
                                                                                        aaGATAAACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAAc          <  2:3075270/101‑1 (MQ=255)
                                                                                           ataaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGGGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGCCGTGGGGACCGCGCCACGtt       >  1:4220084/4‑101 (MQ=25)
                                                                                            taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc      <  1:3560715/99‑1 (MQ=255)
                                                                                            taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc      <  1:2826325/99‑1 (MQ=255)
                                                                                            taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc      <  1:4399733/99‑1 (MQ=255)
                                                                                            taaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTc      >  2:2537661/3‑101 (MQ=255)
                                                                                             aaaCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCa     >  2:57247/2‑101 (MQ=255)
                                                                                               aCGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAgg   <  1:3937225/101‑1 (MQ=255)
                                                                                                cGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGc  >  1:4477216/1‑101 (MQ=255)
                                                                                                |                                                                                                    
GTGTTCCGCCTCGATTGAGGCGGCCCGGGCAGGGGGCGCTGCCCCCTCTTTCGGCTGCGCCGAAATTCACCCCCGGGATATTTGAGGCAAGAT.AACGGGCGCCTATCACCTTGGTGCAAATATCCGGGGGGTGAGGCCGAAAGCCGAGGGGGCAGCGCCCCCTTTTCCCGTCGTGTGGATCGCGCAACGTTCAGGC  >  NC_014034/580862‑581057

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG