Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 66,387 T→G 100% S518S (TCT→TCG flhA → flagellar biosynthesis protein FlhA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403466,3870T→G100.0% 690.6 187S518S (TCT→TCGflhAflagellar biosynthesis protein FlhA
Reads supporting (aligned to +/- strand):  new base (105/82):  ref base (0/0):  total (105/82)

Only 100 of 198 total aligned reads displayed.

GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484
                                                                                                  |                                                                                                 
gTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGaa                                                                                                 >  2:1060113/1‑101 (MQ=255)
gTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGaa                                                                                                 <  1:3996451/101‑1 (MQ=255)
  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGCAGTGTGCAACCTTTCCGATCACGCCCGCGCCGAGTCGAAcc                                                                                               >  2:2044842/1‑101 (MQ=255)
  gATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAcc                                                                                               >  2:345595/1‑101 (MQ=255)
    tCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGGCGAAccgc                                                                                             >  2:1666412/1‑101 (MQ=255)
      cGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAAccgccg                                                                                           >  2:1550116/1‑101 (MQ=255)
       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          >  1:820040/1‑101 (MQ=255)
       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          >  1:3848920/1‑101 (MQ=255)
       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          >  2:1412172/1‑101 (MQ=255)
       ggCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCACCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACcgccgc                                                                                          >  2:2379830/1‑101 (MQ=255)
        gcgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCgccgcc                                                                                         <  1:2716757/101‑1 (MQ=255)
        gcgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCgccgcc                                                                                         >  2:3198406/1‑101 (MQ=255)
        gcgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCgccgcc                                                                                         <  2:827536/101‑1 (MQ=255)
         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        <  2:1354069/101‑1 (MQ=255)
         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        >  2:4328697/1‑101 (MQ=255)
         cgcAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCt                                                                                        >  2:4196934/1‑101 (MQ=255)
           cAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGc                                                                                      >  1:244374/1‑101 (MQ=255)
             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    <  2:486813/101‑1 (MQ=255)
             aCTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCtt                                                                                    >  2:2757154/1‑101 (MQ=255)
              ctccGGCCGTTTGATGACGCTGCGGGCGCTGCGCCGACTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCTCCGAGTCGAACCGCCGCCTGCTTg                                                                                   <  1:2906750/98‑1 (MQ=255)
              cTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTg                                                                                   <  2:3690217/101‑1 (MQ=255)
               ttCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGa                                                                                  <  1:903439/101‑1 (MQ=255)
               ttCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGa                                                                                  >  1:2325288/1‑101 (MQ=255)
                tCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGAc                                                                                 <  1:1548913/101‑1 (MQ=255)
                 cGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGACGAGTCGAACCGCCGCCTGCTTGACg                                                                                >  1:2803546/1‑101 (MQ=255)
                    ccGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGc                                                                             <  2:4144093/101‑1 (MQ=255)
                          gatgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcc                                                                       >  1:3961003/1‑101 (MQ=255)
                           atgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcgg                                                                      <  2:4121224/101‑3 (MQ=255)
                           atgaCGCTGCGGCCGCTGCGCCGCCTGCTCGAGGGAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATccc                                                                      >  1:87758/1‑101 (MQ=255)
                           atgaCGCGGCGGGCGCTGCGCCGCCAGCTCGAGGAAGTGTGCCACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATccc                                                                      <  2:1617994/101‑1 (MQ=255)
                            tgaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATcccc                                                                     >  2:521101/1‑101 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    >  1:4061571/1‑101 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  1:1216213/101‑1 (MQ=255)
                             gaCGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCg                                                                    <  1:1049517/101‑1 (MQ=255)
                               cGCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGAc                                                                  <  1:4579758/101‑1 (MQ=255)
                                gCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACa                                                                 >  2:4446129/1‑101 (MQ=255)
                                gCTGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACa                                                                 <  2:1454775/101‑1 (MQ=255)
                                 gagCGGGAGCTGCGCCGACTGCTCGAGGAAGTGTGCAACCTATCGGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAACTGATCCCCGACaa                                                                <  2:4627393/99‑1 (MQ=255)
                                  tGCGGGCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAg                                                               >  1:2522530/1‑101 (MQ=255)
                                    cGGGCGCTGCGCCGCGTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGt                                                             >  1:3934805/1‑101 (MQ=255)
                                     gggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGtt                                                            <  1:4197222/101‑1 (MQ=255)
                                      ggCGCTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTc                                                           <  1:3434131/101‑1 (MQ=255)
                                       gcgcTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCTTTCCCGCCCGCGGCGACCCCAGCCACCTCCTGCTTGACCAGCTGATCCCCGCCAAGGTTcc                                                          <  2:3622187/101‑1 (MQ=255)
                                       gcgcTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTcc                                                          <  1:3976969/101‑1 (MQ=255)
                                       gcgcTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTcc                                                          <  2:3125743/101‑1 (MQ=255)
                                       gcgcGGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTcc                                                          <  2:244350/101‑1 (MQ=255)
                                          cTGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGt                                                       <  2:3514830/101‑1 (MQ=255)
                                           tGCGCCGCCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTc                                                      >  1:724109/1‑101 (MQ=255)
                                             cgccgcCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGa                                                    >  1:634453/1‑101 (MQ=255)
                                             cgccgcCTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGa                                                    >  2:4268475/1‑101 (MQ=255)
                                              gccgccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGAt                                                   <  1:4608990/101‑1 (MQ=255)
                                                 gccTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCTGACAAGGTTCCGGTCGATctg                                                >  1:1574489/1‑101 (MQ=255)
                                                   cTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              >  2:2542655/1‑101 (MQ=255)
                                                   cTGCTCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgct                                              >  1:1971244/1‑101 (MQ=255)
                                                      ctggAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgct                                           <  1:4599009/98‑1 (MQ=255)
                                                       tCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgctg                                          >  2:2584026/1‑101 (MQ=255)
                                                       tCGAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATctgctgctg                                          >  2:2134792/1‑101 (MQ=255)
                                                         ggggagttctgCACCCTTCCCGTTCCCGCCGGCGCCGAGTCCATCCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        <  2:1582708/92‑1 (MQ=255)
                                                         gAGGAAGTGTGCACCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        <  2:4598282/101‑1 (MQ=255)
                                                         gAGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGc                                        >  2:4245670/1‑101 (MQ=255)
                                                          gggAAGTGTGCAACCTTTCCGACCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       <  2:552522/100‑1 (MQ=255)
                                                          aGGAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCg                                       >  1:846306/1‑101 (MQ=255)
                                                           ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  2:1937880/1‑101 (MQ=255)
                                                           ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  2:1213958/1‑101 (MQ=255)
                                                           ggAAGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCgg                                      >  1:2091405/1‑101 (MQ=255)
                                                            gaagtgtGCATCCTTTCCGAGCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGt                                     <  2:2062309/98‑1 (MQ=255)
                                                            gaagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGt                                     <  1:1681639/98‑1 (MQ=255)
                                                             aagtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtg                                    <  1:3403181/99‑1 (MQ=255)
                                                               gtgtGCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGtgct                                  >  1:4466681/1‑101 (MQ=255)
                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              >  2:3305176/1‑101 (MQ=255)
                                                                   gCAACCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGt                              <  1:4113460/101‑1 (MQ=255)
                                                                     acccTTTCCGATCCCGCCCGGGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTct                            <  1:985844/99‑1 (MQ=255)
                                                                     aaCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTct                            >  2:2826893/1‑101 (MQ=255)
                                                                     aaCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTct                            >  1:2253641/1‑101 (MQ=255)
                                                                     aaCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTct                            >  2:3993167/1‑101 (MQ=255)
                                                                     aaCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTct                            <  2:724034/101‑1 (MQ=255)
                                                                      aCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctg                           <  1:2035205/101‑1 (MQ=255)
                                                                      aCCTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctg                           <  2:1029428/101‑1 (MQ=255)
                                                                       ccTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgc                          <  1:2584200/101‑1 (MQ=255)
                                                                        cTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgct                         >  1:1300629/1‑101 (MQ=255)
                                                                        cTTTCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgct                         >  1:522602/1‑101 (MQ=255)
                                                                         tttCCGATCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTctgctg                        >  1:1767044/1‑101 (MQ=255)
                                                                               aTCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaa                  >  2:4093496/1‑101 (MQ=255)
                                                                                tCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaag                 <  1:2134942/101‑1 (MQ=255)
                                                                                tCCCGCCCGCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaag                 >  2:3445591/1‑101 (MQ=255)
                                                                                 cccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaaga                <  1:4045682/101‑1 (MQ=255)
                                                                                 cccgcccgCGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaaga                <  1:1412267/101‑1 (MQ=255)
                                                                                  ccgcccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGgaagaa               >  1:3649114/1‑101 (MQ=255)
                                                                                      ccgcGCCGAGTCGACCCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACggg           <  2:4127087/101‑1 (MQ=255)
                                                                                      ccgcGCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACggg           >  1:2624237/1‑101 (MQ=255)
                                                                                       cgcgCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACgggg          >  2:2939897/1‑100 (MQ=255)
                                                                                       cgcgCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgt          >  2:4265182/1‑101 (MQ=255)
                                                                                       cgcgCCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgt          >  1:387345/1‑101 (MQ=255)
                                                                                        gctccGAGTCGACCCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtg         <  2:3934520/98‑1 (MQ=255)
                                                                                         cgcCGAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGgtgt        >  2:1823072/1‑101 (MQ=255)
                                                                                             gAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGAt    >  2:910552/1‑101 (MQ=255)
                                                                                             gAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGAt    >  2:2924192/1‑101 (MQ=255)
                                                                                             gAGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGAt    <  2:4484214/101‑1 (MQ=255)
                                                                                              aGTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATc   >  1:1083883/1‑101 (MQ=255)
                                                                                               gTCGAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATcc  <  1:4378592/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GTGATCCGGCGCAACTTCGGCCGTCTGATGACGCTGCGGGCGCTGCGCCGCCTGCTCGAGGACGTGTGCAACCTTTCCGATCCCGCCCGCGCCGAGTCTAACCGCCGCCTGCTTGACGAGCTGATCCCCGACAAGGTTCCGGTCGATCTGCTGCTGGCGGTGCTGCGTCTGCTGCTGGAAGAACGGGTGTCGATCC  >  NC_014034/66289‑66484

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG