Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,809 T→C 8.4% S1600G (AGC→GGC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,8090T→C 8.4% 36.5 ‑0.2 ‑0.4225S1600G (AGC→GGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (11/8):  ref base (107/98):  total (118/107)
Fisher's exact test strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.42e-01

Only 100 of 237 total aligned reads displayed.

GTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGC  >  NC_014034/678709‑678909
                                                                                                    |                                                                                                    
gTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCt                                                                                                      >  1:569263/1‑101 (MQ=255)
  cccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCa                                                                                                    >  2:3742495/1‑101 (MQ=255)
  cccGACCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCa                                                                                                    <  1:519112/101‑1 (MQ=255)
   ccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                                                                                   <  2:2197967/101‑1 (MQ=255)
   ccGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAg                                                                                                   >  2:3829685/1‑101 (MQ=255)
      gtCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCg                                                                                                <  1:2388166/100‑1 (MQ=255)
       tgcctgCCATGGCCGAGACCGAAAGCTGGGCGATAGCCGCGGTTTCCAGCGCCGCCATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGt                                                                                               <  2:530439/96‑1 (MQ=255)
       tCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGt                                                                                               <  1:1568126/101‑1 (MQ=255)
       tCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGt                                                                                               >  2:2821398/1‑101 (MQ=255)
          ccGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgcc                                                                                            >  1:3997205/1‑101 (MQ=255)
           cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                           <  2:1879220/101‑1 (MQ=255)
           cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                           <  2:158425/101‑1 (MQ=255)
           cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                           <  1:2892154/101‑1 (MQ=255)
           cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCAATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                           <  2:67512/101‑1 (MQ=255)
             ccATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgcc                                                                                         <  1:3313316/101‑1 (MQ=255)
              cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                        <  1:3519609/101‑1 (MQ=255)
                tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                                                      <  1:292847/101‑1 (MQ=255)
                tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                                                      <  2:2075514/101‑1 (MQ=255)
                tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTAGCGCTCAGCCGTGCCGCCTgc                                                                                      >  2:229863/1‑101 (MQ=255)
                 ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                                                                                     >  2:4064849/1‑101 (MQ=255)
                   ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGcc                                                                                   <  2:2538473/101‑1 (MQ=255)
                    cGAGCCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                  <  2:1067886/101‑1 (MQ=255)
                    cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                  <  1:3024160/101‑1 (MQ=255)
                      agaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTg                                                                                <  1:3233292/101‑1 (MQ=255)
                       gaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGAGCCGCGATCTGCGCCGTGCTCAGGGCCGCGATCTGCGTCGCGCTCAGGCGAGCCGCCTGCGCCGTgg                                                                               >  1:422044/1‑101 (MQ=38)
                         ccGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTc                                                                             <  1:2486466/101‑1 (MQ=255)
                          cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                                            >  2:2603951/1‑101 (MQ=255)
                          cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                                            <  1:3857900/101‑1 (MQ=255)
                          cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                                            <  1:3829970/101‑1 (MQ=255)
                           gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAg                                                                           >  1:4600027/1‑101 (MQ=255)
                           gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAg                                                                           <  1:3556659/101‑1 (MQ=255)
                            aaaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgc                                                                          >  2:3229676/1‑101 (MQ=255)
                             aaGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAgcg                                                                         >  1:4570082/1‑101 (MQ=255)
                              aGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGACAgcgc                                                                        >  2:618886/1‑101 (MQ=255)
                               gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc                                                                       >  1:2081092/1‑101 (MQ=255)
                                cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa                                                                      >  2:2710580/1‑101 (MQ=255)
                                 tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                                                     <  2:3711649/101‑1 (MQ=255)
                                  gggCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACg                                                                    >  1:1811023/1‑101 (MQ=255)
                                  gggCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACg                                                                    >  2:30580/1‑101 (MQ=255)
                                      gATATCCGCGGTTTCCAGCGCCGCGATCTGAGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt                                                                >  1:4152820/1‑101 (MQ=255)
                                      cagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                                >  2:3509874/4‑101 (MQ=2)
                                       atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc                                                               >  1:1932589/1‑101 (MQ=255)
                                       atatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTc                                                               <  1:2190938/101‑1 (MQ=255)
                                       agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                               <  2:3016308/99‑1 (MQ=2)
                                        tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa                                                              <  1:1731361/101‑1 (MQ=255)
                                        gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                              <  1:171829/100‑1 (MQ=2)
                                         atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt                                                             >  1:359249/1‑101 (MQ=255)
                                         atCCGCGGTTTCCAGCCGCGCGATCTGCGCCGTGCCCAGCGCCGCGATCTGCGGCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt                                                             <  1:2797362/101‑1 (MQ=25)
                                          tcggcgGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAtc                                                            <  1:742343/98‑2 (MQ=2)
                                           ccGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTc                                                           <  1:1670387/101‑1 (MQ=255)
                                             gcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGt                                                         <  1:3778355/101‑1 (MQ=255)
                                             ccgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGt                                                         <  2:4598606/100‑1 (MQ=255)
                                              cgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTg                                                        >  1:2593826/1‑101 (MQ=255)
                                              cgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTg                                                        <  1:861133/101‑1 (MQ=255)
                                               ggTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGc                                                       <  1:4534707/101‑1 (MQ=255)
                                               ggTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGc                                                       >  1:3843862/1‑101 (MQ=255)
                                                 tttCCAGCGCCGCGATCCGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                                                     <  2:4006800/101‑1 (MQ=255)
                                                  ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                                                    >  1:2339620/1‑101 (MQ=255)
                                                   tCCAGCTCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGAGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGGGCCCAg                                                   >  2:878795/1‑101 (MQ=255)
                                                   tCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAg                                                   >  1:3486816/1‑101 (MQ=255)
                                                      aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                <  2:2843889/101‑1 (MQ=255)
                                                      aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                >  2:3137656/1‑101 (MQ=255)
                                                        cgccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCt                                              <  1:2304064/101‑1 (MQ=255)
                                                        cgccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCt                                              >  1:3340056/1‑101 (MQ=255)
                                                         gccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCtt                                             >  1:1156926/1‑101 (MQ=255)
                                                          ccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTc                                            <  2:3375748/101‑1 (MQ=255)
                                                           cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                           >  2:3900663/1‑101 (MQ=255)
                                                           cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                           <  1:3301625/101‑1 (MQ=255)
                                                             cgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgc                                         >  2:200611/1‑101 (MQ=255)
                                                               aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc                                       <  2:1810897/101‑1 (MQ=255)
                                                                tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc                                      <  1:935035/101‑1 (MQ=255)
                                                                 cTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccg                                     <  1:1988874/101‑1 (MQ=255)
                                                                  tGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccgc                                    >  2:2697341/1‑101 (MQ=255)
                                                                  tGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccgc                                    <  1:2756284/101‑1 (MQ=255)
                                                                   gcgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCcgcg                                   >  1:987047/1‑101 (MQ=255)
                                                                        gTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCt                              >  1:3455365/1‑101 (MQ=255)
                                                                         tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc                             >  2:3673812/1‑101 (MQ=255)
                                                                          gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa                            >  1:489260/1‑101 (MQ=255)
                                                                             cAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcg                         >  2:441191/1‑101 (MQ=255)
                                                                              aGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcgc                        <  2:1932454/101‑1 (MQ=255)
                                                                                  ccgcGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCg                    <  1:635031/101‑1 (MQ=255)
                                                                                    gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                  <  1:200635/101‑1 (MQ=255)
                                                                                    gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                  <  2:3270995/101‑1 (MQ=255)
                                                                                     cgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATc                 >  1:1364038/1‑101 (MQ=255)
                                                                                     cgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATc                 <  1:2140455/101‑1 (MQ=255)
                                                                                      gATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATcc                >  1:3110010/1‑100 (MQ=255)
                                                                                        tcggCGTGGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcc              <  2:892483/98‑1 (MQ=255)
                                                                                         cTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGccc             >  1:4382384/1‑101 (MQ=255)
                                                                                          tGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcccc            >  2:3831729/1‑101 (MQ=255)
                                                                                          tGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcccc            >  1:3154908/1‑101 (MQ=255)
                                                                                           gCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCg           <  1:3674675/101‑1 (MQ=255)
                                                                                            cGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCgg          >  2:3606416/1‑101 (MQ=255)
                                                                                               cgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCg       >  2:1845906/1‑101 (MQ=255)
                                                                                               cgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCg       <  1:2740120/101‑1 (MQ=255)
                                                                                                  gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa    >  2:2946654/1‑101 (MQ=255)
                                                                                                  gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa    <  2:2376867/101‑1 (MQ=255)
                                                                                                  gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa    <  1:4268577/101‑1 (MQ=255)
                                                                                                   gtCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAg   <  1:1097167/100‑1 (MQ=255)
                                                                                                    tCAGCTGTGCCGCCTGCGCCGTGCTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGAGCTCAGCGCGCCGAGCGCCCCGGTCGACAgc  >  2:755809/1‑101 (MQ=25)
                                                                                                    tCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgc  <  2:1561288/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GTCCCGATCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGC  >  NC_014034/678709‑678909

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG