Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,816 T→C 8.4% A1597A (GCA→GGC RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,8160T→C 8.4% 35.7 ‑0.2 ‑0.3226A1597A (GCA→GCGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (11/8):  ref base (109/98):  total (120/106)
Fisher's exact test strand distribution p-value = 8.11e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.24e-01

Only 100 of 237 total aligned reads displayed.

TCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCCA  >  NC_014034/678716‑678916
                                                                                                    |                                                                                                    
tCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGt                                                                                                      >  2:2821398/1‑101 (MQ=255)
tCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGt                                                                                                      >  2:3252072/1‑101 (MQ=255)
    cGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCAATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccg                                                                                                  <  2:67512/101‑1 (MQ=255)
     gCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgc                                                                                                 >  2:3519351/1‑101 (MQ=255)
      ccATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTgccgcc                                                                                                <  1:3313316/101‑1 (MQ=255)
       cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                               <  1:3519609/101‑1 (MQ=255)
       cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                               <  1:4266357/101‑1 (MQ=255)
       cATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCt                                                                                               <  1:2067231/101‑1 (MQ=255)
         tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                                                             <  1:292847/101‑1 (MQ=255)
         tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgc                                                                                             <  2:2075514/101‑1 (MQ=255)
         tGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTAGCGCTCAGCCGTGCCGCCTgc                                                                                             >  2:229863/1‑101 (MQ=255)
          ggCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTgcg                                                                                            <  2:1198880/101‑1 (MQ=255)
            ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGcc                                                                                          <  2:2538473/101‑1 (MQ=255)
            ccGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGcc                                                                                          <  2:3633053/101‑1 (MQ=255)
             cGAGCCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                         <  2:1067886/101‑1 (MQ=255)
             cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                         >  1:1976969/1‑101 (MQ=255)
             cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                         >  1:282058/1‑101 (MQ=255)
             cGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCg                                                                                         <  1:3024160/101‑1 (MQ=255)
              gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                                                        <  1:2768329/101‑1 (MQ=255)
              gagaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGt                                                                                        <  1:4544469/101‑1 (MQ=255)
               agaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTg                                                                                       >  1:1907328/1‑101 (MQ=255)
               agaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTg                                                                                       <  1:3233292/101‑1 (MQ=255)
                gaCCGAAAGCTGGGCGATATCCGCGGTTTCCAGAGCCGCGATCTGCGCCGTGCTCAGGGCCGCGATCTGCGTCGCGCTCAGGCGAGCCGCCTGCGCCGTgg                                                                                      >  1:422044/1‑101 (MQ=38)
                 aCCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGt                                                                                     <  1:1201677/101‑1 (MQ=255)
                   cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                                                   <  1:3857900/101‑1 (MQ=255)
                   cGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCa                                                                                   >  2:2603951/1‑101 (MQ=255)
                    gAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAg                                                                                  >  1:4600027/1‑101 (MQ=255)
                       aGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGACAgcgc                                                                               >  2:618886/1‑101 (MQ=255)
                        gCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGcc                                                                              >  1:2081092/1‑101 (MQ=255)
                         cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCa                                                                             >  2:2710580/1‑101 (MQ=255)
                         cTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCACCa                                                                             >  1:333341/1‑101 (MQ=255)
                          tGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCAc                                                                            <  2:3711649/101‑1 (MQ=255)
                          cgcaccagatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                                            >  2:1619522/9‑101 (MQ=2)
                           gggCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACg                                                                           >  2:30580/1‑101 (MQ=255)
                             gCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGAt                                                                         >  2:3365931/1‑101 (MQ=255)
                               gATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGt                                                                       >  1:1738526/1‑101 (MQ=255)
                                agatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                      <  2:3016308/99‑1 (MQ=2)
                                 tatCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCa                                                                     <  1:2005976/101‑1 (MQ=255)
                                 gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                                     <  1:171829/100‑1 (MQ=2)
                                 gatCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACCATGTCa                                                                     >  1:667987/2‑101 (MQ=2)
                                  atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                                                    <  1:3501679/101‑1 (MQ=2)
                                  atCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                                                    >  1:848824/1‑101 (MQ=2)
                                  atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt                                                                    >  1:359249/1‑101 (MQ=255)
                                  atCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAt                                                                    <  1:1542886/101‑1 (MQ=255)
                                     cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg                                                                 >  1:240099/1‑101 (MQ=255)
                                     cgcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCg                                                                 <  2:1907193/101‑1 (MQ=255)
                                      gcgGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGt                                                                <  1:3778355/101‑1 (MQ=255)
                                        ggTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGc                                                              >  2:3809353/1‑101 (MQ=255)
                                         gTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGcc                                                             <  1:229887/101‑1 (MQ=255)
                                          tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                                                            >  1:2586946/1‑101 (MQ=255)
                                          tttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGccc                                                            <  1:4509094/101‑1 (MQ=255)
                                           ttCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCa                                                           >  1:2339620/1‑101 (MQ=255)
                                            tCCAGCTCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGAGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGGGCCCAg                                                          >  2:878795/1‑101 (MQ=255)
                                             ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGc                                                         <  1:625575/101‑1 (MQ=255)
                                               aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                       >  2:3137656/1‑101 (MQ=255)
                                               aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                       <  2:2843889/101‑1 (MQ=255)
                                               aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGccc                                                       <  1:4065135/101‑1 (MQ=255)
                                                  gccgcGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCtt                                                    >  1:1156926/1‑101 (MQ=255)
                                                    cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                                  >  2:3900663/1‑101 (MQ=255)
                                                    cgcgATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCa                                                  <  1:3301625/101‑1 (MQ=255)
                                                       gATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcg                                               <  1:2163121/101‑1 (MQ=255)
                                                        aTCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAgcgc                                              <  2:1810897/101‑1 (MQ=255)
                                                         tCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgcc                                             <  1:935035/101‑1 (MQ=255)
                                                           tGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGcgccgc                                           >  2:2697341/1‑101 (MQ=255)
                                                            gcgcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCcgcg                                          >  1:987047/1‑101 (MQ=255)
                                                              gcCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGt                                        <  2:348398/101‑1 (MQ=255)
                                                                 gTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCt                                     <  1:480/101‑1 (MQ=255)
                                                                 gTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCt                                     >  1:3455365/1‑101 (MQ=255)
                                                                  tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc                                    >  2:3673812/1‑101 (MQ=255)
                                                                  tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc                                    >  2:4096940/1‑101 (MQ=255)
                                                                  tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc                                    >  1:3812955/1‑101 (MQ=255)
                                                                  tGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCAACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTc                                    >  2:2037215/1‑101 (MQ=255)
                                                                   gCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCAATCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCa                                   >  1:3975718/1‑101 (MQ=255)
                                                                    cTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAg                                  <  2:3228184/101‑1 (MQ=255)
                                                                    cTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAg                                  <  1:3838004/101‑1 (MQ=255)
                                                                       aGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAgcgc                               <  2:1932454/101‑1 (MQ=255)
                                                                             gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                         <  1:3674087/101‑1 (MQ=255)
                                                                             gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                         <  2:4569783/101‑1 (MQ=255)
                                                                             gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                         <  1:2628924/101‑1 (MQ=255)
                                                                             gcgATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGAt                         >  2:4613336/1‑101 (MQ=255)
                                                                                 tcggCGTGGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcc                     <  2:892483/98‑1 (MQ=255)
                                                                                  cTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGccc                    >  1:1728213/1‑101 (MQ=255)
                                                                                  cTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGccc                    >  1:4382384/1‑101 (MQ=255)
                                                                                   tGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcccc                   >  1:3154908/1‑101 (MQ=255)
                                                                                   tGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGcccc                   >  2:3831729/1‑101 (MQ=255)
                                                                                        cgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCg              <  1:2740120/101‑1 (MQ=255)
                                                                                        cgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCg              >  2:1845906/1‑101 (MQ=255)
                                                                                        cgcgCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCg              <  2:4035769/101‑1 (MQ=255)
                                                                                         gcgcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGa             <  1:512661/101‑1 (MQ=255)
                                                                                           gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa           <  2:2376867/101‑1 (MQ=255)
                                                                                           gcTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACa           >  2:2946654/1‑101 (MQ=255)
                                                                                            gtCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAg          <  1:1097167/100‑1 (MQ=255)
                                                                                             tCAGCTGTGCCGCCTGCGCCGTGCTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGAGCTCAGCGCGCCGAGCGCCCCGGTCGACAgc         >  2:755809/1‑101 (MQ=25)
                                                                                             tCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgc         >  1:3093616/1‑101 (MQ=255)
                                                                                             tCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgc         <  2:1561288/101‑1 (MQ=255)
                                                                                               aGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAgcgc       >  1:2233394/1‑101 (MQ=255)
                                                                                                gCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgcc      <  1:280433/101‑1 (MQ=255)
                                                                                                gCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgcc      >  1:1666059/1‑101 (MQ=255)
                                                                                                  cGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGcgccgc    >  1:1607992/1‑101 (MQ=255)
                                                                                                    tGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCCa  >  2:546748/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
TCCCCGCCATGGCCGAGACCGAAAGCTGGGCGATATCCGCGGTTTCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCGATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTCATTCGTGCCCAGCCCCTTCAGCGCCGCGGTGCTCAGCGCGCCGATCGCCCCGGTCGACAGCGCCGCCA  >  NC_014034/678716‑678916

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG