Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,149 T→G 10.8% M820L (ATC→CTC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,1490T→G10.8% 25.1 ‑3.6 ‑0.0111M820L (ATC→CTC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (11/1):  ref base (57/42):  total (68/43)
Fisher's exact test strand distribution p-value = 2.69e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.91e-01

Only 100 of 118 total aligned reads displayed.

GCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCA  >  NC_014034/681053‑681249
                                                                                                |                                                                                                    
gCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcg                                                                                                  >  2:1208874/1‑100 (MQ=25)
 cTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGTCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgc                                                                                                 >  2:1793744/1‑99 (MQ=25)
 cTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgc                                                                                                 >  2:4287412/1‑99 (MQ=25)
  tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                                                >  1:2917857/1‑98 (MQ=25)
   gcgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcca                                                                                               >  2:1730515/1‑97 (MQ=25)
     gcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccact                                                                                             >  1:1237129/1‑95 (MQ=25)
     cccGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccact                                                                                             <  1:829920/100‑7 (MQ=21)
       cGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactgg                                                                                           >  1:4219663/1‑93 (MQ=21)
        gTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggc                                                                                          >  1:1619573/1‑92 (MQ=21)
         tGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggcc                                                                                         >  2:1414237/1‑91 (MQ=255)
         tGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggcc                                                                                         >  1:4254928/1‑91 (MQ=25)
                                                 ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGTCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                                                 >  1:3921526/11‑101 (MQ=37)
                                                 ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                                                 >  2:1736550/11‑101 (MQ=37)
                                                 ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                                                 >  2:2361205/11‑101 (MQ=37)
                                                  tgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgt                                                >  2:2230505/10‑101 (MQ=38)
                                                   gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  2:3294009/93‑1 (MQ=255)
                                                   gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  1:1556247/93‑1 (MQ=255)
                                                   gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCCAGCCCCTTGATCGCATTGGCACCCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  1:3987090/93‑1 (MQ=37)
                                                    cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg                                              >  1:1892995/8‑101 (MQ=255)
                                                    cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg                                              >  1:1154046/8‑101 (MQ=255)
                                                     gccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcgt                                             <  1:1613432/95‑1 (MQ=255)
                                                      ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                                            <  1:2105024/96‑1 (MQ=255)
                                                      ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCAATGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                                            >  2:4037655/6‑101 (MQ=38)
                                                       cgtcgttaGCGCCACGATGTCATCGGTGCCGATGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                                           <  2:2776385/94‑1 (MQ=38)
                                                       cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                                           <  1:3260145/97‑1 (MQ=255)
                                                        gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt                                          >  1:768814/4‑101 (MQ=255)
                                                         tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         <  2:3443084/99‑1 (MQ=255)
                                                         tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         >  1:2245014/3‑101 (MQ=255)
                                                         tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         <  1:2048137/99‑1 (MQ=255)
                                                          cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  1:2330521/100‑1 (MQ=255)
                                                          cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  1:3194457/100‑1 (MQ=255)
                                                           gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       >  1:1785196/1‑101 (MQ=255)
                                                           ctCAGCGCCACGATGTCATCGGTGCCGAGGGCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       <  2:3315582/100‑1 (MQ=255)
                                                            tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc                                      >  2:245392/1‑101 (MQ=255)
                                                            tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc                                      >  1:2285044/1‑101 (MQ=255)
                                                             cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                                     >  1:2296602/1‑101 (MQ=255)
                                                              aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    <  2:2748494/101‑1 (MQ=255)
                                                               gcgcCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGcgcg                                   <  2:3760670/101‑1 (MQ=255)
                                                                 gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc                                 <  2:657350/98‑1 (MQ=255)
                                                                 gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc                                 >  2:3595334/4‑101 (MQ=255)
                                                                  ccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGcc                                <  2:1206446/99‑1 (MQ=255)
                                                                   cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa                               >  1:2652255/2‑101 (MQ=255)
                                                                   cacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCa                               >  1:4408468/2‑101 (MQ=255)
                                                                    acgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCAt                              >  2:3529150/1‑101 (MQ=255)
                                                                    acgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGCGCTCAGCGCGGCCAt                              <  1:3932219/101‑1 (MQ=255)
                                                                      gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  2:2959579/1‑101 (MQ=255)
                                                                      gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  1:2125816/1‑101 (MQ=255)
                                                                      gaTGTCATCGGTGCCGAGGCCATTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  2:818859/1‑101 (MQ=255)
                                                                       aTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTg                           <  2:848748/101‑1 (MQ=255)
                                                                        tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          <  1:716051/101‑1 (MQ=255)
                                                                         gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  2:1790010/1‑101 (MQ=255)
                                                                         gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  1:4211027/1‑101 (MQ=255)
                                                                         gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  2:3662541/1‑101 (MQ=255)
                                                                         gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  2:4067765/1‑101 (MQ=255)
                                                                         gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  2:4419048/1‑101 (MQ=255)
                                                                           cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGcc                       >  2:3315253/1‑101 (MQ=255)
                                                                           cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGcc                       >  2:1094543/1‑101 (MQ=255)
                                                                            aTCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCg                      <  1:2410835/101‑1 (MQ=255)
                                                                              cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    >  1:1098011/1‑101 (MQ=255)
                                                                              cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    >  2:469006/1‑101 (MQ=255)
                                                                              cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    <  1:4445219/101‑1 (MQ=255)
                                                                              cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    <  2:4048565/101‑1 (MQ=255)
                                                                              cGGTGCCGACGCGCTTGATCGCATTTGCAATCAGCGCCATCACGCTGGCCGTATTCTGGGCCGCAAGCTGCGTCGTGCTCAGCGCGTCCATCTGCGCCGAg                    <  2:2722488/101‑1 (MQ=255)
                                                                               ggTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAgg                   >  2:3277211/1‑101 (MQ=255)
                                                                               ggTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAgg                   >  2:467740/1‑101 (MQ=255)
                                                                                gTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGt                  <  2:1519610/101‑1 (MQ=255)
                                                                                 tGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTc                 <  2:2527417/101‑1 (MQ=255)
                                                                                  gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  1:1406649/101‑1 (MQ=255)
                                                                                  gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  1:1096744/101‑1 (MQ=255)
                                                                                   ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               >  1:3161563/1‑101 (MQ=255)
                                                                                   ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               <  1:3021370/101‑1 (MQ=255)
                                                                                    cgccgCCGTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGc              >  2:2465845/5‑101 (MQ=255)
                                                                                    cGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGc              <  1:2043169/101‑1 (MQ=255)
                                                                                    cGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGc              >  1:2770649/1‑101 (MQ=255)
                                                                                     gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  2:4048230/1‑101 (MQ=255)
                                                                                     gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  2:3016846/1‑101 (MQ=255)
                                                                                     gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  1:4656373/1‑101 (MQ=255)
                                                                                      ccgCCGTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            <  1:2466011/99‑1 (MQ=255)
                                                                                      aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGTCCATCTGCGCCGAGGTCAGccc            <  2:4525252/101‑1 (MQ=255)
                                                                                      aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            >  2:1590980/1‑101 (MQ=255)
                                                                                      aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            >  1:238392/1‑101 (MQ=255)
                                                                                      aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            >  1:2062472/1‑101 (MQ=255)
                                                                                       ggCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGTCTGTATTCAGCGCGCCACGCCGCGGCGTGCTCAGCGCGCCCATCTGCGCCGAGGTCCGCCcg           <  2:798760/101‑1 (MQ=255)
                                                                                       ggCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcg           >  1:2820967/1‑101 (MQ=255)
                                                                                       ggCCCTAGATCGCACTGGCACTCAGCGCCGTCAGGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcg           <  1:4023744/101‑1 (MQ=255)
                                                                                        gCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgc          <  2:607421/101‑1 (MQ=255)
                                                                                        gCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgc          >  2:4577477/1‑101 (MQ=255)
                                                                                         cccTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgcg         >  2:3889437/1‑101 (MQ=255)
                                                                                         cccTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgcg         >  2:3669756/1‑101 (MQ=255)
                                                                                          ccTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCgcgc        >  2:2283112/1‑101 (MQ=255)
                                                                                            ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      >  2:1376202/1‑101 (MQ=255)
                                                                                            ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      <  1:4406203/101‑1 (MQ=255)
                                                                                            ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      >  1:1579084/1‑101 (MQ=255)
                                                                                            ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      <  1:2298392/101‑1 (MQ=255)
                                                                                             tGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAcc     >  2:3573376/1‑101 (MQ=255)
                                                                                              gATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCg    <  1:483303/101‑1 (MQ=255)
                                                                                              gATCGCATTGGCACTCACCGCCGTCACGCTGGCGCTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCg    <  1:4005358/101‑1 (MQ=255)
                                                                                               aTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGTGGTCAGCCCGCGCACCGc   >  2:3072901/1‑101 (MQ=255)
                                                                                               aTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGc   >  1:3476789/1‑101 (MQ=255)
                                                                                                tCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCCTCTGCGCCGAGGCCAGCCCGCGCACCGCa  >  1:1381251/1‑101 (MQ=255)
                                                                                                |                                                                                                    
GCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCA  >  NC_014034/681053‑681249

Base quality scores:  ATCG  < 3 ≤  ATCG  < 24 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG