Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,502 C→G 25.4% R702P (CGT→CCT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,5020C→G25.4% 140.8 ‑2.8 0.0220R702P (CGT→CCT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (21/35):  ref base (86/78):  total (107/113)
Fisher's exact test strand distribution p-value = 6.35e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

CAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGC  >  NC_014034/681402‑681602
                                                                                                     |                                                                                                    
cagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAc                                                                                                      >  2:345035/1‑101 (MQ=255)
  gcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAcgc                                                                                                    >  1:4426277/1‑101 (MQ=255)
     cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgcc                                                                                                 <  2:3065080/101‑1 (MQ=255)
      ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccg                                                                                                <  1:2775615/101‑1 (MQ=255)
      ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccg                                                                                                >  1:4200161/1‑101 (MQ=255)
       gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccgc                                                                                               <  2:2897994/101‑1 (MQ=255)
         gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCt                                                                                             <  2:2739261/101‑1 (MQ=255)
           gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCTCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCTGCCTgc                                                                                           <  2:2299833/101‑1 (MQ=255)
               cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                                       >  1:109252/1‑101 (MQ=255)
                    cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                                                  <  1:3797802/101‑1 (MQ=255)
                    cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                                                  >  1:4240731/1‑101 (MQ=255)
                     gcTGATCTGCGCCAGCTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                                                 <  1:4392501/101‑1 (MQ=255)
                      gtGACCTGCGCCAGCTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                                                <  2:4567994/100‑1 (MQ=255)
                      cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                                                <  2:3355794/101‑1 (MQ=255)
                      cTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                                                <  1:2243131/101‑1 (MQ=255)
                       tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                                               <  2:3964048/101‑1 (MQ=255)
                        gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAggg                                                                              >  1:2286080/1‑99 (MQ=255)
                         agccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgcgc                                                                             <  2:501424/94‑1 (MQ=2)
                         agccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgcgc                                                                             >  2:2410673/8‑101 (MQ=2)
                         aTCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcgc                                                                             >  2:1554628/1‑101 (MQ=255)
                          tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                                            <  1:3668210/101‑1 (MQ=255)
                          gccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                                            <  2:2861264/95‑1 (MQ=2)
                            cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                                          <  1:1572588/97‑1 (MQ=2)
                               accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                                       >  2:3554258/2‑101 (MQ=2)
                                  aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                                    >  1:3879822/1‑101 (MQ=255)
                                   gacccgccgCCCCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                                   <  1:1641566/98‑1 (MQ=255)
                                   gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                                   >  2:4289623/1‑101 (MQ=255)
                                       ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               >  2:3211112/11‑101 (MQ=9)
                                       ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               >  1:3315831/11‑101 (MQ=9)
                                       ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                               >  1:286960/11‑101 (MQ=9)
                                        gccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCTCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                                              >  2:2964995/1‑101 (MQ=255)
                                         cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                             <  1:2542221/93‑1 (MQ=9)
                                         ccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                             <  1:4330497/101‑1 (MQ=255)
                                          ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                                            >  2:4067314/8‑101 (MQ=9)
                                          ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                                            <  1:4306509/94‑1 (MQ=9)
                                          cgccTGGAGGGCCGCGATCTGCGTGCTGGTCAGCGCACCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                                            <  2:3758005/101‑1 (MQ=255)
                                           gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           >  2:773499/7‑101 (MQ=9)
                                           gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  1:262082/95‑1 (MQ=9)
                                           gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           >  2:3502189/1‑101 (MQ=255)
                                           gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           >  1:458677/1‑101 (MQ=255)
                                           gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                                           <  1:2391168/101‑1 (MQ=255)
                                            ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa                                                          <  1:2124375/101‑1 (MQ=255)
                                             ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCTCCACGATGTCATCGGTGccga                                                         <  2:219624/97‑3 (MQ=9)
                                             ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                                         >  1:2681679/5‑99 (MQ=2)
                                             ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                                         >  1:851601/5‑99 (MQ=9)
                                             cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                                                         >  1:2082330/1‑101 (MQ=255)
                                              tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCTAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                        <  1:3924149/101‑1 (MQ=255)
                                              tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                                        >  1:4064842/1‑101 (MQ=255)
                                               cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                       <  1:2158278/101‑1 (MQ=255)
                                               cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                       >  1:622181/1‑101 (MQ=255)
                                               cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg                                                       >  2:2554466/1‑101 (MQ=255)
                                                caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                                      >  1:1965656/2‑96 (MQ=2)
                                                caGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                                      >  1:1743236/2‑96 (MQ=9)
                                                 ggcgcCGCGACCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                                     <  2:215413/100‑1 (MQ=255)
                                                 aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                     >  2:2794383/1‑101 (MQ=2)
                                                 aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                                     >  1:1259292/1‑101 (MQ=2)
                                                  gcgcCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccc                                                    <  1:586247/101‑2 (MQ=9)
                                                   cgccgcAACCTGCGTGCGCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                                   <  2:867686/101‑3 (MQ=2)
                                                    gccgcGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                                                  >  1:1388195/1‑101 (MQ=255)
                                                    gccgcGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                                                  >  2:4420740/1‑101 (MQ=255)
                                                    gacgcgATCTGCGTGCTGGTCGGGGGGCCG.ATCTGCGCCGAGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                                                  <  2:4478699/99‑1 (MQ=21)
                                                      cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                                                >  1:3146235/1‑101 (MQ=255)
                                                      cgcgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGa                                                <  1:1554733/101‑1 (MQ=255)
                                                        cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                                              <  2:2591340/101‑1 (MQ=255)
                                                         gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                             >  1:814188/1‑101 (MQ=255)
                                                          aTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                                            >  2:2214444/1‑101 (MQ=255)
                                                           tCTGCTTGCTGTTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                                           <  2:1426763/101‑1 (MQ=255)
                                                           tCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                                           <  2:2681195/101‑1 (MQ=255)
                                                            cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                                          <  1:1115076/101‑1 (MQ=255)
                                                              gCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                                        <  2:2353201/101‑1 (MQ=255)
                                                               cGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGccgccg                                       >  2:418058/1‑101 (MQ=255)
                                                                  gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                                    <  1:3611068/101‑1 (MQ=255)
                                                                   cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                                   >  2:3307172/1‑101 (MQ=255)
                                                                    tGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                                  <  1:281598/101‑1 (MQ=255)
                                                                    tGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                                  >  1:914778/1‑101 (MQ=255)
                                                                     ggTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                                 >  2:1270008/1‑101 (MQ=255)
                                                                      gTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAg                                <  1:4550589/101‑1 (MQ=255)
                                                                           cccgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccg                           <  2:1032194/99‑1 (MQ=255)
                                                                              gcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                        >  2:162564/1‑101 (MQ=255)
                                                                              gcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                        >  1:2191635/1‑101 (MQ=255)
                                                                              gcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa                        >  1:29892/1‑101 (MQ=255)
                                                                                       tcgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGcc                <  2:1086922/100‑1 (MQ=255)
                                                                                       gcgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGcc                <  1:1492521/101‑1 (MQ=255)
                                                                                        cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg               >  2:1166015/1‑101 (MQ=255)
                                                                                        cgcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACAATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCg               <  2:4022997/101‑1 (MQ=255)
                                                                                         gcCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGa              >  2:382028/1‑101 (MQ=255)
                                                                                          ccGAGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGaa             <  2:2790489/101‑1 (MQ=255)
                                                                                          ccGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGaa             <  1:1078106/101‑1 (MQ=255)
                                                                                             ggCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGcc          <  1:345067/100‑1 (MQ=255)
                                                                                             aGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGTCACCGTCGCCGAAGcc          <  2:1190596/101‑1 (MQ=255)
                                                                                              gCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCa         >  1:4521414/1‑101 (MQ=255)
                                                                                              gCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAACGGTGCCAAAGCCGCGGATTGCTGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCa         >  1:3989073/1‑101 (MQ=255)
                                                                                               cccAGCCCCCCCCCCCGCGCCGTCGTCAGCCCCCCGATGTCATCGGTGCCAAACCCGCGGACCCCCCCCGTGCCCAGCCCCCCCCCCGTCGCCGAAGCCaa        <  2:3957075/101‑2 (MQ=255)
                                                                                                ccAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAgc       >  2:1883472/1‑101 (MQ=255)
                                                                                                   gccgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc    <  2:2623988/99‑1 (MQ=255)
                                                                                                   gccgccCCCCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc    <  2:1388100/99‑1 (MQ=255)
                                                                                                   gACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgcc    <  1:2827840/101‑1 (MQ=255)
                                                                                                    ccgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccg   <  2:424728/100‑1 (MQ=255)
                                                                                                    aCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccg   <  1:418095/101‑1 (MQ=255)
                                                                                                     cgcgCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGcgccgc  <  2:438659/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGC  >  NC_014034/681402‑681602

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG