Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,665 A→C 11.5% S648A (TCC→GCC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,6650A→C11.5% 58.8 ‑2.5 ‑0.1201S648A (TCC→GCC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (16/7):  ref base (88/89):  total (105/96)
Fisher's exact test strand distribution p-value = 8.04e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.64e-01

Only 100 of 216 total aligned reads displayed.

GTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGC  >  NC_014034/681565‑681765
                                                                                                    |                                                                                                    
gTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCGGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGga                                                                                                      >  2:3735433/1‑101 (MQ=255)
gTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGga                                                                                                      >  1:42228/1‑101 (MQ=255)
 tGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgag                                                                                                     >  2:3697236/1‑101 (MQ=255)
  gCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGgaga                                                                                                    >  1:2273336/1‑101 (MQ=255)
   cccAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGagag                                                                                                   <  1:4324838/101‑1 (MQ=255)
     cAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcg                                                                                                 <  1:3649980/101‑1 (MQ=255)
      aGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcgc                                                                                                <  1:983521/101‑1 (MQ=255)
      aGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcgc                                                                                                >  1:1331225/1‑101 (MQ=255)
      aGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAgcgc                                                                                                >  2:115583/1‑101 (MQ=255)
         cccgccCCCGTCGCCGAGCCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                             <  2:2082190/100‑1 (MQ=255)
         cccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                             <  2:317753/100‑1 (MQ=255)
          ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                                                                                            >  2:1819719/1‑101 (MQ=255)
           cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGa                                                                                           <  1:1270101/101‑1 (MQ=255)
            gccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc                                                                                          >  1:2152962/1‑101 (MQ=255)
             ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGCcc                                                                                         >  1:3313278/1‑101 (MQ=255)
              cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt                                                                                        >  1:382138/1‑101 (MQ=255)
               cccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg                                                                                       <  2:4565953/100‑1 (MQ=255)
               aCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg                                                                                       >  1:510826/1‑101 (MQ=255)
               aCCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTg                                                                                       <  1:3768763/101‑1 (MQ=255)
                  gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc                                                                                    <  1:1023784/101‑1 (MQ=255)
                     gCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGt                                                                                 <  2:2239686/101‑1 (MQ=255)
                      ccaaaGCCAGCGCCGCGATCTGCGCCTTGCTCAGCTCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTg                                                                                <  1:2505074/98‑1 (MQ=255)
                       ggtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGc                                                                               >  1:235464/10‑101 (MQ=2)
                       ggtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGc                                                                               >  1:3694257/10‑101 (MQ=2)
                       cGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGc                                                                               >  1:2092394/1‑101 (MQ=255)
                       cGAACCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGc                                                                               <  1:4398100/101‑1 (MQ=255)
                         tggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGccc                                                                             >  2:570930/8‑99 (MQ=2)
                         aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc                                                                             >  2:4016245/1‑101 (MQ=255)
                         aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATAGGTGCTc                                                                             >  2:3130733/1‑101 (MQ=255)
                          ggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                            <  1:3282690/95‑1 (MQ=2)
                          ggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                            >  1:1752945/7‑101 (MQ=2)
                           gCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAg                                                                           <  2:42222/101‑1 (MQ=255)
                           gCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAg                                                                           <  1:1997451/101‑1 (MQ=255)
                            ccAGCGCCGCGATCGGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGc                                                                          <  2:3205324/101‑1 (MQ=255)
                            caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                          <  2:326761/97‑2 (MQ=2)
                             cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                         >  2:376650/1‑101 (MQ=255)
                             aacccccccgATCTGCCCCGCGCCCAGCCCCGCGATCTGCCCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                         <  2:3714353/93‑3 (MQ=255)
                              atcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgact                                                                        >  1:1116513/3‑98 (MQ=2)
                              agggccgcGATCTGCGCCGTGCTCAGCTCCGCCGCCTGCACCGAGCCAAGCCCGGCCCCCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCtt                                                                        <  1:2780262/98‑1 (MQ=255)
                               gcgcCGCGATCTGCGCCTTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                       <  1:42691/101‑1 (MQ=255)
                               gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                       <  1:87054/101‑1 (MQ=255)
                                 gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                     <  2:3755173/101‑1 (MQ=255)
                                  ccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCg                                                                    <  1:3735708/101‑1 (MQ=255)
                                   cgcgATCTGCGCCGCGCTCGGCGCCGCGATCGGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                   <  1:3520286/101‑9 (MQ=2)
                                   cgcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                   <  1:4575630/101‑9 (MQ=2)
                                     cgATCTGCGCCGTGCTCAGCGCGGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATc                                                                 <  2:2018014/101‑1 (MQ=255)
                                     cgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATc                                                                 >  1:3455011/1‑101 (MQ=255)
                                     cgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccagc                                                                 >  2:2785727/1‑91 (MQ=2)
                                     cgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccagc                                                                 >  2:2183914/1‑91 (MQ=2)
                                      gATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                <  2:2332850/101‑1 (MQ=255)
                                       aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                               >  1:4097415/1‑101 (MQ=255)
                                       aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                               >  2:361181/1‑101 (MQ=255)
                                       aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                               >  1:297540/1‑101 (MQ=255)
                                        tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                              >  1:4549406/1‑101 (MQ=255)
                                        tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                              >  1:1095313/1‑101 (MQ=255)
                                         ttGCGCCGTGCTCAGCGCCGCCGCTTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCg                                                             <  2:2563820/100‑1 (MQ=255)
                                         cTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGccaaatcgga                                                             <  2:183316/101‑9 (MQ=255)
                                         cTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCg                                                             <  2:899221/101‑1 (MQ=255)
                                          tGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGt                                                            <  1:1409185/101‑1 (MQ=255)
                                          tGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGt                                                            >  1:4456304/1‑101 (MQ=255)
                                           gcgcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTc                                                           <  2:3936244/101‑1 (MQ=255)
                                             gcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgc                                                         >  2:3395630/1‑101 (MQ=255)
                                              ccGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgcg                                                        <  2:2152812/101‑1 (MQ=255)
                                              ccGTGCTCAGCCCCCCCGCCTGCGCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCCCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgcg                                                        <  2:746154/101‑1 (MQ=255)
                                                gTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCt                                                      >  2:4404767/1‑101 (MQ=255)
                                                gTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCt                                                      >  1:802385/1‑101 (MQ=255)
                                                   cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                   >  2:3639841/1‑101 (MQ=255)
                                                    tCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAGACTGGCCAGCTGCATCGTGCCCAgc                                                  >  2:1077151/1‑100 (MQ=0)
                                                    tCAGCGCCGCCGCCTGCCCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAgg                                                  <  2:1434973/101‑1 (MQ=255)
                                                          ccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaa                                            >  1:3661384/1‑101 (MQ=255)
                                                           cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                           <  1:2583574/101‑1 (MQ=255)
                                                           cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                           >  1:39936/1‑101 (MQ=255)
                                                            gccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                          >  1:2687503/1‑101 (MQ=255)
                                                              cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccg                                        >  2:4274378/1‑101 (MQ=255)
                                                                  tGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACcgccgc                                    >  2:2469281/1‑101 (MQ=255)
                                                                  tGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACcgccgc                                    >  2:3813845/1‑101 (MQ=255)
                                                                   gCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACcgccgcc                                   >  2:3013414/1‑100 (MQ=255)
                                                                        gAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGCGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGcc                              >  2:416275/1‑101 (MQ=255)
                                                                         aGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGccc                             <  1:660370/101‑1 (MQ=255)
                                                                          gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                            <  1:1579349/101‑1 (MQ=255)
                                                                          gCCAACCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                            <  2:1491157/101‑1 (MQ=255)
                                                                            cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCAGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGc                          >  2:2616153/1‑101 (MQ=255)
                                                                             aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                         <  2:229745/101‑1 (MQ=255)
                                                                                cccGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcg                      >  1:902547/1‑101 (MQ=255)
                                                                                cccGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcg                      >  2:2983668/1‑101 (MQ=255)
                                                                                 ccGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgc                     <  1:1675628/101‑1 (MQ=255)
                                                                                  cGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgcg                    <  1:3130977/101‑1 (MQ=255)
                                                                                  cGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgcg                    <  2:3387628/101‑1 (MQ=255)
                                                                                   ggCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGa                   <  2:1331132/101‑1 (MQ=255)
                                                                                     ccACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATc                 <  2:3454757/101‑1 (MQ=255)
                                                                                       aCCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTg               <  1:3209063/101‑1 (MQ=255)
                                                                                           cAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGcc           >  2:4531378/1‑101 (MQ=255)
                                                                                           cAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGACCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGcc           >  2:2720714/1‑101 (MQ=255)
                                                                                            aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGGCCTTCGcct          >  2:1695416/1‑100 (MQ=255)
                                                                                            aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg          >  2:3509204/1‑101 (MQ=255)
                                                                                             aGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGt         >  2:870868/1‑101 (MQ=255)
                                                                                                 tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc     <  1:1526985/101‑1 (MQ=255)
                                                                                                 tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc     >  2:2877329/1‑101 (MQ=255)
                                                                                                   gagaGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAg   <  2:4097129/101‑1 (MQ=255)
                                                                                                    agagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGc  <  1:3236317/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GTGCCCAGCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGC  >  NC_014034/681565‑681765

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG