Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 74,095 A→C 100% F196L (TTT→TTG trmE ← tRNA modification GTPase TrmE

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403474,0950A→C100.0% 965.0 252F196L (TTT→TTGtrmEtRNA modification GTPase TrmE
Reads supporting (aligned to +/- strand):  new base (123/129):  ref base (0/0):  total (123/129)

Only 100 of 265 total aligned reads displayed.

AGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGAAAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAGCG  >  NC_014034/73997‑74192
                                                                                                  |                                                                                                 
aGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGacaa                                                                                                 <  1:380540/101‑1 (MQ=255)
aGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGacaa                                                                                                 >  2:2978540/1‑101 (MQ=255)
aGAAGCGTCGATTTCCCGGCAGTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGacaa                                                                                                 >  1:2649218/1‑101 (MQ=255)
   aGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTg                                                                                              <  1:3315934/101‑1 (MQ=255)
   aGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTg                                                                                              >  2:3411694/1‑101 (MQ=255)
    gCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgc                                                                                             <  2:2297605/101‑1 (MQ=255)
    gCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgc                                                                                             <  1:2926144/101‑1 (MQ=255)
    gCGTCGATTTCCCGGCANTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgc                                                                                             <  2:1123103/101‑1 (MQ=255)
     cGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTgcg                                                                                            >  1:3007806/1‑101 (MQ=255)
           tttCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCaa                                                                                      >  1:1602696/1‑101 (MQ=255)
               ccGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTc                                                                                  <  2:3096287/101‑1 (MQ=255)
                cGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCg                                                                                 >  1:1967406/1‑101 (MQ=255)
                 ggCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCgg                                                                                >  1:2427137/1‑101 (MQ=255)
                   cATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAc                                                                              <  2:220200/101‑1 (MQ=255)
                    aTTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAca                                                                             <  1:3528606/101‑1 (MQ=255)
                    aTTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAca                                                                             <  2:2261022/101‑1 (MQ=255)
                     ttcgatcgccCGACGATCGCAACTTCAAAGCCTTCCCCCCCCCGTTCGGTCACCGCAATCCGGCCGATTTCCGCCGACAACTGCGACGCAACCTCGGaaca                                                                            >  2:919920/7‑98 (MQ=255)
                       cGGCGGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGACCGATTTCCGCCGACAACTGCGCCGCAACCTCGGAcagca                                                                          <  2:4067263/101‑1 (MQ=255)
                         gccgccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACagcagc                                                                        >  2:1260127/1‑101 (MQ=255)
                         gccgccCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACagcagc                                                                        >  2:4311072/1‑101 (MQ=255)
                             cccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaa                                                                    >  2:4520991/1‑101 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   <  1:363566/101‑1 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   <  2:339461/101‑1 (MQ=255)
                              ccGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaa                                                                   >  1:1392588/1‑101 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  1:689896/101‑1 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  1:115983/101‑1 (MQ=255)
                               cgacgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCaaaa                                                                  <  2:1437924/101‑1 (MQ=255)
                                 acgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGc                                                                >  1:2288309/1‑101 (MQ=255)
                                  cgaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGcc                                                               <  1:4431396/101‑1 (MQ=255)
                                   gaTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCt                                                              <  1:573664/101‑1 (MQ=255)
                                    aTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTc                                                             >  2:3594364/1‑101 (MQ=255)
                                    aTCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTc                                                             >  1:2811586/1‑101 (MQ=255)
                                     tCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTcc                                                            <  2:1538021/101‑1 (MQ=255)
                                      cGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCg                                                           <  2:3341922/101‑1 (MQ=255)
                                      cGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCg                                                           <  2:301622/101‑1 (MQ=255)
                                       gCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCgg                                                          <  2:1303301/101‑1 (MQ=255)
                                        cAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGc                                                         >  1:2579615/1‑101 (MQ=255)
                                            ttCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGtta                                                     >  1:1524346/1‑99 (MQ=255)
                                            ttCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTca                                                     >  2:1824867/1‑101 (MQ=255)
                                             tCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTcac                                                    >  1:1004285/1‑101 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  <  2:31287/101‑1 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  >  1:3680232/1‑101 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  >  1:4287461/1‑101 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  <  1:4506745/101‑1 (MQ=255)
                                               aaaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGAACAGCAGCGCAAAAGCCTCCGGCGTCACAt                                                  >  1:3856677/1‑101 (MQ=255)
                                                aaGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATc                                                 >  1:3409439/1‑101 (MQ=255)
                                                 aGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCa                                                <  2:2020217/101‑1 (MQ=255)
                                                  gCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCaa                                               >  2:3650551/1‑101 (MQ=255)
                                                   ccTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAc                                              >  2:2845966/1‑101 (MQ=255)
                                                    cTTCCCGCCCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAAcc                                             <  1:4455482/101‑1 (MQ=255)
                                                      gcccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCgg                                           <  2:603327/100‑1 (MQ=255)
                                                       cccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGc                                          <  2:3475688/101‑1 (MQ=255)
                                                        ccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCCCATCAACCGGCa                                         >  2:3228040/1‑101 (MQ=255)
                                                        ccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCa                                         >  2:785092/1‑101 (MQ=255)
                                                        ccGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCa                                         >  2:265779/1‑101 (MQ=255)
                                                         ccgaCCCGTTCGGTCACCGAAATCCGGCCCATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAg                                        <  2:141053/98‑1 (MQ=255)
                                                         cGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAg                                        >  2:4662366/1‑101 (MQ=255)
                                                         cGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAg                                        <  1:2960370/101‑1 (MQ=255)
                                                          gcccccGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAgg                                       <  1:484083/98‑1 (MQ=255)
                                                          gCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAgg                                       >  1:1794816/1‑101 (MQ=255)
                                                          cgatctgTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAgg                                       <  1:997098/95‑1 (MQ=255)
                                                            aCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTc                                     >  2:4235433/1‑101 (MQ=255)
                                                            aCCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTc                                     >  1:3424402/1‑101 (MQ=255)
                                                               cGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTc                                  <  1:4320192/101‑1 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  2:1420715/1‑101 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  1:4650697/1‑101 (MQ=255)
                                                                gTTCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCa                                 >  1:3234636/1‑101 (MQ=255)
                                                                 ttCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCAt                                <  2:1392493/101‑1 (MQ=255)
                                                                 ttCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCAt                                <  2:1602591/101‑1 (MQ=255)
                                                                 ttCGGTCACCAAAATCCGGCCGATTTCCGCCGACTACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCAt                                <  2:1083090/101‑1 (MQ=255)
                                                                  tCGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGGCTTAATc                               >  2:2946865/1‑101 (MQ=255)
                                                                   cGGTCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCg                              <  2:2080671/101‑1 (MQ=255)
                                                                      tCACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCg                           <  1:883420/101‑1 (MQ=255)
                                                                       cACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGa                          >  1:622650/1‑101 (MQ=255)
                                                                       cACCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGa                          >  2:3795458/1‑101 (MQ=255)
                                                                        aCCGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGaa                         <  2:2516325/101‑1 (MQ=255)
                                                                          cGAAATCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAAt                       <  2:1710915/101‑1 (MQ=255)
                                                                            aaaTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCg                     <  1:3260106/101‑1 (MQ=255)
                                                                             aaTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGCCTTCATCGGCGAAATCGa                    >  1:2279642/1‑101 (MQ=255)
                                                                              aTCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGAt                   >  2:1209575/1‑101 (MQ=255)
                                                                               tCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATg                  >  2:4204268/1‑101 (MQ=255)
                                                                               tCCGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATg                  <  1:3714345/101‑1 (MQ=255)
                                                                                ccGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGc                 <  2:4420733/101‑1 (MQ=255)
                                                                                 cGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCa                <  1:1260220/101‑1 (MQ=255)
                                                                                 cGGCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCa                >  1:2713618/1‑101 (MQ=255)
                                                                                  ggCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAg               <  2:957826/101‑1 (MQ=255)
                                                                                  ggCCGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAg               <  1:4531025/101‑1 (MQ=255)
                                                                                    ccGATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGc             <  1:3802106/101‑1 (MQ=255)
                                                                                      gATTTCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCt           <  1:176740/101‑1 (MQ=255)
                                                                                        tttCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTcc         <  2:443893/101‑1 (MQ=255)
                                                                                         ttCCGCCGACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCa        >  1:1132163/1‑101 (MQ=255)
                                                                                           ccgccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATc      >  1:4172101/1‑101 (MQ=255)
                                                                                           ccgccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATc      >  2:3958185/1‑101 (MQ=255)
                                                                                           ccgccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGCCTTCATCGGCGAAATCCATGCAGGCCTCCATc      >  2:4148966/1‑101 (MQ=255)
                                                                                            cgccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCa     <  2:283130/101‑1 (MQ=255)
                                                                                             gccgACAACTGCGCCGCAACCTCGGANAGCNGCGAAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAg    <  1:2773122/101‑1 (MQ=255)
                                                                                             gccgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAg    >  1:1673418/1‑101 (MQ=255)
                                                                                               cgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATGAgcc  >  2:1898731/1‑100 (MQ=255)
                                                                                               cgACAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAgcg  >  2:857662/1‑101 (MQ=255)
                                                                                               cgACAACTGCGCCGCAACCTAGGACAGCAGCGCAAAAACATCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAAACAATGCAGGCCCACATCacac  >  2:447612/1‑98 (MQ=255)
                                                                                                  |                                                                                                 
AGAAGCGTCGATTTCCCGGCATTCGGCCGCCCGACGATCGCAACTTCAAAGCCTTCCCGCACCCGTTCGGTCACCGAAATCCGGCCGATTTCCGCCGAAAACTGCGCCGCAACCTCGGACAGCAGCGCAAAAGCCTCCGGCGTCACATCAACCGGCAGGTCTTCATCGGCGAAATCGATGCAGGCCTCCATCAGCG  >  NC_014034/73997‑74192

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG